Polo Universitario dellAnnunziata

Messina, Italy

Polo Universitario dellAnnunziata

Messina, Italy
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Morabito R.,Messina University | Remigante A.,Messina University | Cavallaro M.,Polo Universitario Dellannunziata | Taormina A.,Veterinary | And 2 more authors.
Pflugers Archiv European Journal of Physiology | Year: 2017

Band 3 protein efficiency in mediating Cl−/HCO3 − exchange through erythrocytes membrane is reduced by oxidative stress. The aim of the present study was to verify whether and how anion transport through band 3 protein may be useful in monitoring canine leishmaniasis (Leishmania infantum) development, a disease associated to membrane protein degradation and oxidative stress. To accomplish this aim, serological analysis to determine IFAT (immunofluorescence antibody test) titers against leishmaniasis has been performed and 1:160 and 1:540 titers, determined at diagnosis and after 6 months, were considered for experiments. Oxidative conditions have been assessed by estimating MDA (malondialdehyde) plasma levels, intracellular GSH (reduced glutathione) content, and membrane –SH groups. Band 3 protein anion exchange capability was evaluated by measuring the rate constant for SO4 = uptake, and its expression levels, along with those of P-Tyr (phosphorylated tyrosine), involved in pathways underlying band 3 protein function, have been also determined. Our results show that, in infected dogs with 1:160 IFAT titer, high MDA plasma levels and oxidation of –SH groups are associated to increased P-Tyr expression levels, leading to a reduction in anion exchange capability throughout 6 months of diagnosis. On the other hand, infected dogs with 1:540 IFAT titer, exhibited oxidative conditions associated to an impaired anion exchange capability at diagnosis, were ameliorated after 6 months. Such findings suggest that (1) band 3 protein-mediated anion transport is reduced by oxidative conditions associated to leishmaniasis, putatively via phosphorylative pathways; (2) band 3 protein efficiency may account for canine leishmaniasis development; and (3) the assessment of band 3 protein function may represent an additional tool for canine leishmaniasis diagnosis and monitoring of its development, with potential application to humans, either in case of leishmaniasis or other oxidative-related pathologies. © 2017 Springer-Verlag Berlin Heidelberg


Mugnaini L.,Prophylaxis and Food Hygiene | Papini R.,Prophylaxis and Food Hygiene | Gorini G.,Environmental and Occupational Epidemiology Unit | Passantino A.,Polo Universitario DellAnnunziata | And 2 more authors.
Revue de Medecine Veterinaire | Year: 2012

The pattern of endoparasite infections was examined in 273 cats (181 owned cats, 54 sheltered cats, and 28 stray cats from colonies) by combining results of different diagnostic techniques. Overall, 26.4% of cats harboured one (for 86.1% of positive cats) or more endoparasites including Toxocara cati (12.1%), Cystoisospora felis (2.9%), Aelurostrongylus abstrusus (2.2%), C. felis/T. cati and Cystoisospora rivolta (1.8% each), Ancylostoma tubaeforme (1.1%), C. rivolta/T. cati, Dipylidium caninum, Eucoleus aerophilus and Toxascaris leonina (0.7% each), A. abstrusus/D. caninum/T. cati, A. tubaeforme/ T. cati, C. felis/D. caninum, and Cryptosporidium felis (0.4% each). Tritrichomonas foetus was not detected. In univariate analysis, predictive factors of endoparasitism included being male, intact, less than 1-year-old, stray or owned-outdoor, untreated, and fed occasionally with homemade food. Multivariate analysis showed that males were 4.25 times more likely to be infected if they were less than 1-year-old and 3.21 times if they did not receive periodical antiparasite treatments while females were 10.43 more likely to be infected if showing intestinal or respiratory clinical signs. These data may be of importance for veterinary practitioners to evaluate the occurrence of endoparasitism in cats.


Chiofalo B.,Polo Universitario dellAnnunziata | Riolo E.B.,Polo Universitario dellAnnunziata | Fasciana G.,C.da Zotta Caldaia | Liotta L.,Polo Universitario dellAnnunziata | Chiofalo V.,Polo Universitario dellAnnunziata
Veterinary Research Communications | Year: 2010

The increasing demand for animals from organic breeding systems has increased interest in certain natural substances, called nutraceuticals, to stimulate the organic defenses of the animals. The aim of this trial was to study the effects of dietary rosemary extract in 36 ewes, from 57 to 154 days of lactation, divided into three groups: CTR (basal diet), ROXLD (600 mg extract/head/day) and ROXHD (1,200 mg extract/head/day). A significantly higher quantity of milk and quantitative daily production of protein, casein, fat, and lactose were observed in the milk of animals in the ROXHD group compared with milk from animals in the CTR and ROXLD groups. No significant differences were observed for somatic cell counts, considering that treated groups showed lower values compared with controls. A significant decrease in clotting time (r) and increase in curd firmness (a30) were observed in milk of both treated groups (ROXLD and ROXHD) compared with the CTR group. These results could be related to the significantly higher acidity values, pH and SH°, observed in the milk of animals from the treated groups. Dietary rosemary extract in dairy sheep enhanced milk yield, quality, and renneting properties due to its "natural, functional ingredients." © Springer Science+Business Media B.V. 2010.


Lanteri G.,Polo Universitario dellAnnunziata | Marino F.,Polo Universitario dellAnnunziata | Liotta L.,Messina University | Stefano C.,Polo Universitario dellAnnunziata | MacRi B.,Polo Universitario dellAnnunziata
Acta Veterinaria Hungarica | Year: 2011

In this study, an experimental challenge was carried out by feeding Nero Siciliano pigs with acorn to evoke melanin-like pigmentation and support the hypothesis that it is caused by ingested material. Twelve pigs were studied, 6 fed with acorns and 6 fed commercial feed. At slaughter, all the animals fed on acorns showed black discolouration of almost all lymph nodes. The lymph nodes were normal in size and shape. Histochemical tests performed on tissues allowed us to identify and differentiate the pigment. Immunohistochemical staining for macrophage markers showed macrophages containing a variable amount of melanin-like granules. Our data, and the well-known steps of melanin formation, confirm that swine enzymes could act on phenolic substances found in acorns.


Lanteri G.,Polo Universitario dellAnnunziata | Sfacteria A.,Polo Universitario dellAnnunziata | MacRi D.,Polo Universitario dellAnnunziata | Reale S.,Instituto Zooprofilattico Sperimentale Della Sicilia A Mirri | Marino F.,Instituto Zooprofilattico Sperimentale Della Sicilia A Mirri
Journal of Zoo and Wildlife Medicine | Year: 2011

Penicillium mycosis, or penicilliosis, is rare in animals. This report describes the clinical signs and pathological, immunohistochemical, and biomolecular findings in a case of avian penicilliosis in a Congo African grey parrot. On gross necropsy, pyogranulomatous changes were identified in the lung, liver, and kidney. Histopathology demonstrated periodic acid Schiff-(PAS) and Grocott-positive hyphae in these tissues. Immunohistochemistry confirmed the genus of the fungus and polymerase chain reaction (PCR) confirmed a high homology with Penicillium chrysogenum. Copyright 2011 by American Association of Zoo Veterinarians.


Moravec F.,Academy of Sciences of the Czech Republic | Gaglio G.,Polo Universitario dellAnnunziata | Giannetto S.,Polo Universitario dellAnnunziata | Marino F.,Polo Universitario dellAnnunziata
Parasitology Research | Year: 2010

A new nematode species, Philometra spicarae sp. n. (Philometridae), is described from a gravid female found in the abdominal cavity of spicarel, Spicara smaris (Linnaeus) (Centracanthidae, Perciformes), from the Ionian Sea off Sicily, Italy. The new species differs from most other Philometra spp. parasitic in the abdominal cavity of marine fishes by the oesophagus without an anterior inflation, resembling thus only Philometra cheilopogoni Mordvinova, 1986 and Philometra justinei Moravec, Ternengo and Levron, 2006; these two species can be differentiated from P. spicarae by the relative length of the oesophagus to body length (0.6-0.8% vs. 6%) and some other features. From the gonad-infecting species Philometra filiformis (Stossich, 1896), a parasite of Pagellus erythrinus (Linnaeus), P. spicarae differs mainly in having a conspicuously long (1.4 mm) intestinal ligament. It is the first nominal philometrid species described from the fish of the family Centracanthidae. © 2010 Springer-Verlag.


Gaglio G.,Polo Universitario dellAnnunziata | Gaglio G.,University of Bristol | Allen S.,Gower Bird Hospital | Bowden L.,University of Bristol | And 2 more authors.
European Journal of Wildlife Research | Year: 2010

Seventy-four European hedgehogs (Erinaceus europaeus) that had died in wildlife rehabilitation centres were dissected and their parasite burdens documented. Overall parasite prevalence was 91%, and a total of six helminth species were isolated: five nematodes (Crenosoma striatum, Eucoleus aerophilus, Capillaria erinacei, Capillaria ovoreticulata and Capillaria spp.), one trematode (Brachylaemus erinacei) and one acanthocephalan (Oliganthorhynchus erinacei). The tick Ixodes hexagonus and flea Archeopsylla erinacei were also collected. The effect of parasite infection on body condition was assessed by correlation of burdens with the residuals of weight-skeletal length regression. Tick presence was positively related to body condition; for other parasites, no significant relationship was found. Faecal egg or larval count was closely correlated with adult parasite burden for C. striatum and Capillaria/Eucoleus spp., but not for other species. Coprological analysis should therefore be useful for in vivo studies of nematode parasite infection in hedgehogs. The epidemiology of parasites in hedgehogs and their possible role in recent population declines are discussed. © 2010 Springer-Verlag.


Conte F.,Polo Universitario dellAnnunziata | Scarantino S.,Polo Universitario dellAnnunziata
International Food Research Journal | Year: 2013

Colostrum, as a bioactive substance for humans, could be a functional food ingredient; today colostrum products are the object of health claims, even on line, especially from some foreign Countries. The aim of the present paper is to assess the quality of bovine colostrum samples taken in some farms in Sicily and in Calabria, in view of lack of information on physical and chemical and safety criteria on this product for human consumption. Comparing the overall picture of parameters under study, a relationship between these criteria was not easy, due to several factors, as for example animals breed, diversified farms management and other intrinsic and extrinsic additional factors relating to colostrum sampling. The aspects observed in the present study would suggest a great accuracy by competent authority during the qualitative controls of raw colostrum.


PubMed | Polo Universitario dellAnnunziata
Type: Case Reports | Journal: Journal of zoo and wildlife medicine : official publication of the American Association of Zoo Veterinarians | Year: 2012

Penicillium mycosis, or penicilliosis, is rare in animals. This report describes the clinical signs and pathological, immunohistochemical, and biomolecular findings in a case of avian penicilliosis in a Congo African grey parrot. On gross necropsy, pyogranulomatous changes were identified in the lung, liver, and kidney. Histopathology demonstrated periodic acid Schiff-(PAS) and Grocott-positive hyphae in these tissues. Immunohistochemistry confirmed the genus of the fungus and polymerase chain reaction (PCR) confirmed a high homology with Penicillium chrysogenum.


PubMed | Polo Universitario dellAnnunziata
Type: Case Reports | Journal: Diseases of aquatic organisms | Year: 2016

A wild adult male conger Conger conger, captured by a SCUBA diver in the waters of coastal Italy, was sent for laboratory analysis due to the presence of multiple productive ulcerous skin lesions localized in the dorso-lateral body area, caudally to the gill operculum under the dorsal fin. The main mass was sessile, ulcerated and hemorrhaging in appearance and was surrounded by several smaller masses, which originated peripherally from the same mass or were isolated, always with a tendency to ulceration. Histology confirmed that the newly formed tissue originated from derma invading the closer tissues. The tumour consisted of spindle cells, each with an ovoidal nucleus and rarely with evident nucleoli, which were arranged in parallel or storiform patterns and were often surrounding blood-filled spaces discontinuously lined by endothelial cells. Tumour tissue was richly vascularized and no mitoses were seen. The overlying epidermis was ulcerated. Massons trichrome technique indicated the presence of a small amount of perivascular connective tissue. No excessive glycogen storage, bacteria, virus or fungi were detected by periodic acid-Schiff (PAS)-reaction. Immunohistochemistry showed dot-like or diffuse cytoplasmic positivity against smooth muscle actin and the monoclonal antibody D2-40. CD34 exhibited relevant immunoreactivity at plasma membranes. Growth fraction evaluated using MIB-1 was <1%. Immunoreactions for wide spectrum CK, CK5/6, CK8, CK18, EMA, desmin, myoglobin, S-100, CD20, CD68, GFAP, and NSE were negative. Histopathological and immunohistochemical results supported a diagnosis of angioleiomyoma, a benign tumour of the muscular cellular component of the blood vessels. To our knowledge, this is the first report of such neoplasms in fishes in which monoclonal antibodies work on fish tissues, facilitating a useful immunohistochemical approach for differential diagnosis.

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