Polo Regional Centro Oeste

Bauru, Brazil

Polo Regional Centro Oeste

Bauru, Brazil
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De Arruda Palharini M.C.,Polo Regional Centro Oeste | De Julio Pereira Santos C.A.,Ministerio da Agricultura | De Souza Fileti M.,Polo Regional Centro Oeste | Simionato E.M.R.S.,Sacred Heart University of Brazil | Sasaki F.F.C.,Embrapa Mandioca e Fruticultura
Comunicata Scientiae | Year: 2017

The objective of this study was to evaluate the efficacy of sanitization with hydrogen peroxide in reducing the enzymatic browning and pathogens of fresh cut snap bean. Fresh cut snap beans were inoculated by immersion in an Escherichia coli and Salmonella enteritidis contaminant solution Then, the vegetable was sanitized with hydrogen peroxide (0; 5; 10 and 20 mL L-1) for 10 minutes. The pathogens were monitored immediately after sanitization and after 4 days of storage at 10°C. In other similar experiment, but without inoculation of pathogens, the snap bean were evaluated periodically. The surface color, pH, activity of phenylalanine ammonia-lyase, peroxidase and polyphenoloxidase enzymes, chlorophyll and carotenoids contents were evaluated. The sanitization with hydrogen peroxide reduced the count of E. coli and S. enteritidis, being similar for different hydrogen peroxide concentrations. The browning of the section surface of snap bean was controlled by hydrogen peroxide in the concentration of 20 mL L-1. The activity of the enzymes as well as the pigments content (chlorophyll and carotenoids) were not affected by treatments, except the polyphenoloxidase enzyme. The pH increased during storage and was greatest in snap bean sanitized with 20 mL L-1. The sanitization with hydrogen peroxide is effective in reducing population of E.coli and S. enteritidis and minimizing browning in fresh cut snap bean, but caused spots on the vegetable skin, precluding its use.


Dias D.C.,São Paulo State University | Furlaneto F.P.B.,Polo Regional Centro Oeste | Ayroza L.M.S.,Polo Regional Medio Paranapanema | Tachibana L.,Fishery Institute | And 2 more authors.
Acta Scientiarum - Animal Sciences | Year: 2012

This study aimed to analyze the economic feasibility of supplement probiotics Bacillus subtilis to "matrinxã" Brycon amazonicus, raised in cages. The experiment was conducted at the Polo Regional Vale do Ribeira, in Pariquera-açu municipality, São Paulo State, Brazil, from February to July 2009. A total of 960 juvenile matrinxã were stocked in twelve 2.7 m3-net cages (1.5 x 1.5 x 1.2 m), in ponds with a total area of 600 m2 and an average depth of 1.50 m. The tests were conducted with a control treatment (T1) and two probiotic doses (T2 = 5 g and T3 = 10 g kg-1 of diet) with four replicates. Results showed that T2 produced better economic performance for matrinxã at the juvenile stage in intensive rearing system.


De Arruda Palharini M.C.,Polo Regional Centro Oeste | Fischer I.H.,Polo Regional Centro Oeste | Da Cruz Vegian M.R.,São Paulo State University | De Souza Fileti M.,Polo Regional Centro Oeste | Montes S.M.N.M.,Agencia Paulista de Tecnologia dos Agronegocios
Pesquisa Agropecuaria Tropical | Year: 2015

Effect of storage temperature on blackberry postharvest conservation. Blackberries have a relatively short shelf life, due to their fragile structure, high metabolism and incidence of diseases, thus requiring a careful storage. This study aimed at evaluating the effect of different temperatures on blackberry (Brazos cultivar) conservation, characterizing its physical and chemical attributes and quantifying the pathogens responsible for rottenness. A completely randomized experimental design, in a factorial scheme (temperatures x orchards), with three replications of 10 fruits per treatment, was used. The temperatures of 2 ºC and 5 ºC were effective in delaying the color change and degradation of organic acids. There was a significant increase in rottenness incidence with increasing storage temperature. Incidences lower than 7% were observed at the end of storage at 2 ºC and 5 ºC, however, they exceeded 20% at temperatures from 15 ºC. The main pathogens detected were Cladosporium spp. and Colletotrichum spp. Refrigeration at 2 ºC and 5 ºC is an efficient alternative to maintain the quality of blackberries up to nine days, for delaying fruit ripening and rottenness development. © Universidade Federal De Goias (UFG). All rights reserved.


de Arruda M.C.,Polo Regional Centro Oeste | Jacomino A.P.,Escola Superior de Agricultura Luiz de Queiroz | Trevisan M.J.,Escola Superior de Agricultura Luiz de Queiroz | Jeronimo E.M.,Polo Regional Centro Oeste | Moretti C.L.,Embrapa Hortalicas
Bragantia | Year: 2011

The present work aimed to determine the influence of modified atmosphere packaging on the quality maintenance of minimally processed 'Pera' oranges stored under refrigeration. Peeled oranges were placed on packaging materials and were submitted to the following treatments: (1) control - oranges placed in expanded polystyrene trays covered with PVC film; (2) passive - oranges wrapped in 32 μm-polypropylene film under passive modified atmosphere; (3) active - oranges wrapped in 32 μm-polypropylene film under active modified atmosphere (5% O2 + 10% CO2; balance N2). Minimally processed fruits were then stored at 6±1°C and 12±1°C. Chemical, sensorial and microbiological analyses were carried out periodically. Ethanol and acetaldehyde levels, as well as the peroxidase activity were determined. Chemical variables were not affected by treatments. However, it was observed an effect of storage time on the soluble solids content, which reduced throughout the storage period by 10%. Microbiological contamination levels were low and not affected by treatments. There was an accumulation of acetaldehyde and ethanol due to the modified atmosphere. However, it did not affect fruit flavor, which remained acceptable until the end of the storage period, as well as the appearance. Peroxidase activity was low and not influenced by treatments. PVC film was as effective as the polypropylene one under passive or active modified atmosphere within the period evaluated, with oranges stored at 6 °C and 12 °C showing shelf life of 12 and nine days, respectively.


Braga A.R.C.,Mineiro Institute of Agriculture IMA | Langoni H.,São Paulo State University | Lucheis S.B.,Polo Regional Centro Oeste
Journal of Venomous Animals and Toxins Including Tropical Diseases | Year: 2014

Background: This study aimed to evaluate the occurrence ofLeishmaniaspp. in dogs and cats from Botucatu, São Paulo state, and Campo Grande, Mato Grosso do Sul state, Brazil, by the association of three diagnostic tests: blood culture in liver infusion tryptose medium, immunofluorescent antibody test and polymerase chain reaction. Fifty blood samples of dogs and cats from the Center for Zoonosis Control in Campo Grande, an area endemic for canine visceral leishmaniasis, were collected randomly, as well as canine and feline blood samples from the Municipal Kennel and Animal Protection Association in Botucatu, currently considered a transmission-free, non-endemic area.Results: Of the 50 dog blood cultures from Botucatu, three (6%) were positive and of the 50 cats, two (4%) were positive. In Campo Grande, 29 dog blood cultures (58%) were positive and all (100%) cats negative by this test. Polymerase chain reaction detectedLeishmaniaspp. in 100% of dog and cat samples from Botucatu but found all the cats from Campo Grande to be negative. On the other hand, 36 dogs from Campo Grande were positive (72%) by the same technique. Immunofluorescent antibody test in Botucatu found 100% of dogs and cats non-reactive, while in Campo Grande, it detected positivity in 32 dogs (64%) and 15 cats (30%).Conclusions: The results show the importance of not only continuous epidemiological surveillance in areas not endemic for leishmaniasis, but also research for accurate diagnosis of this zoonosis. © 2014 Braga et al.


Galli J.A.,Polo Regional Centro Norte | Fischer I.H.,Polo Regional Centro Oeste | Palharini M.C.A.,Polo Regional Centro Oeste | Michelotto M.D.,Polo Regional Centro Norte
Pesquisa Agropecuaria Tropical | Year: 2015

Among the postharvest diseases that occur in guava, anthracnose, black spot and stem-end rot are the most common. The incidence and diversity of these diseases depend on the host genotype. This study aimed to identify and quantify the postharvest diseases of guava accessions cultivated in organic system. For the characterization of diseases, 30 fruits from 48 accessions were evaluated in a completely randomized design. Anthracnose and black spot were the most frequent postharvest diseases in guava. The Vermelha Redonda (Shimoda), L5P21, L3P12, EEF-3, IAC-4 - Unesp and Monte Alto - Comum 1 accessions stood out with the lowest incidence of anthracnose and black spot, while L4P13, L1P2 and Creme Arredondada (Unesp) showed the highest incidence of anthracnose, black spot and stem-end rot. The Fusicoccum sp., Phomopsis sp., Phoma sp., Dothiorella sp. and Lasiodiplodia sp. pathogens were identified as the main responsible for causing stem-end rot symptoms. © 2015, Universidade Federal De Goias (UFG). All rights reserved.


Firmino A.C.,São Paulo State University | Fischer I.H.,Polo Regional Centro Oeste | Tozze Junior H.J.,Coordenadoria de Defesa Agropecuaria | Rosa D.D.,São Paulo State University | Furtado E.L.,São Paulo State University
Summa Phytopathologica | Year: 2016

The Fusicoccum genus of fungi are known to cause stem-end rot in various fruit plants, such as mango, guava, peach and avocado. Several species of this fungus are reported attacking avocado (Persea americana) in several countries. Based on this information, the present study aimed to identify species of Fusicoccum associated with rot in avocado fruits in the State of São Paulo. Samples were collected (fruits with rot symptoms) from regions of Bauru, Bernadino de Campos and Piraju. All isolates obtained had its pathogenicity confirmed by inoculation of healthy avocado fruits. After confirming its pathogenicity, these isolates had their DNA extracted and the ITS-5.8S rDNA region was amplified. After editing, these sequences were used to search for similar sequences in the NCBI. Eleven samples were identified as Neofusicoccum parvum and others were identified as Botryosphaeria dothidea (F. aesculi). Both species were found in all regions of collection. © 2016, Universidade Estadual Paulista (UNESP). All Rights Reserved.


Palharini M.C.A.,Polo Regional Centro Oeste | Fischer I.H.,Biologa | Alves A.R.O.F.,Polo Regional Centro Oeste | Fileti M.S.,University of Sao Paulo
Revista Brasileira de Fruticultura | Year: 2016

This study aimed to evaluate the effect of alternative products and thermotherapy, singly and in combination, on the physicochemical quality and controlling decay of ‘Pedro Sato’ guavas. Initially the treatments with calcium chloride, potassium phosphite, 1-methylcyclopropene (1-MCP), cassava starch, ethanol followed by chlorine (ethanol+chlorine) and thermotherapy were evaluated for eight days of fruits storage at 22°C. In a second step, four treatments were selected (cassava starch, 1-MCP, thermotherapy and ethanol+chlorine) and evaluated the association of two, applied sequentially, during eight days of fruits storage at 25 °C. The 1-MCP treatment and cassava starch were the most effective in keeping the physicochemical quality of ‘Pedro Sato’ guavas, slowing the weight loss, change of skin color, and contributing to firmness retention. Greater reduction in fruit rot was found in thermotherapy, cassava starch and 1-MCP treatments. The associations of 1-MCP/cassava starch and thermotherapy/cassava starch were the most effective treatments to maintain the quality of ‘Pedro Sato’ guavas, slowing the weight loss, change of skin color, and loss of firmness. Association treatments reduced incidence of decay, especially the ethanol+chlorine/ thermotherapy and thermotherapy/cassava starch that were more efficient until the fourth day of storage. The rots incidence correlated with parameters of skin color and firmness for most association treatments. In general, it is recommended the thermotherapy/cassava starch for guavas stored at 22-25ºC as a way of maintaining the physicochemical quality and delaying at least two days the symptoms of decay. © 2016, Sociedade Brasileira de Fruticultura. All rights reserved.


Palharini M.C.A.,Polo Regional Centro Oeste | Santos C.A.J.P.,Polo Regional Centro Oeste | Simionato E.M.R.S.,Sacred Heart University of Brazil | Kodawara R.K.,ESALQ | Kluge R.A.,ESALQ
Horticultura Brasileira | Year: 2015

The sanitization of processed vegetable should be carried out in order to obtain a microbiologically safe food. Among sanitizers, chlorine, in its various forms, is the most widely used in food. The use of chlorine as chlorine dioxide is interesting since it does not react with the organic material, avoiding formation of trihalomethanes, which have been identified as carcinogens. Also chlorine dioxide is effective against pathogenic organisms and can minimize browning reactions.The objective of this study was to evaluate the efficacy of sanitization with chlorine dioxide to reduce the enzymatic browning and the microorganisms in minimally processed snap bean. Minimally processed snap bean samples were inoculated by immersion in contaminant solution of E. coli and Salmonella. Afterwards, the product was sanitized with chlorine dioxide (0; 50; 100 and 200 mg/L) for 10 minutes. The pathogens were monitored immediately after sanitization during four days of storage at 10ºC. In other experiment, minimally processed snap bean samples were sanitized with chlorine dioxide (0; 50; 100 and 200 mg/L) for 10 minutes and stored at 10ºC and evaluated periodically during seven days of storage. The surface color, pH, activity of the enzymes phenylalanine ammonia-lyase (PAL), peroxidase (POD) and polyphenol oxidase (PPO), chlorophyll and carotenoids content, and microbiological aspects were evaluated. The sanitization with chlorine dioxide reduced the counting of E. coli and Salmonella. This reduction was proportional to the increase of chlorine dioxide concentration. Reduction in psychrotrophic bacteria was also noticed in snap bean sanitized with 100 and 200 mg/L. Counting of total coliforms decreased with increasing of the sanitizing solution. The thermotolerant coliform counting was low in all treatments, reaching a maximum of 2.4x10-1 NMP/g and Salmonella was not detected in the samples. The browning of the section surface of snap bean was not controlled by chlorine dioxide, being similar in all treatments, as well as activity of the enzymes related to browning (PAL, POD and PPO) and content of pigments (chlorophyll and carotenoids). Chlorine dioxide was not effective in minimizing browning in minimally processed snap bean, but is effective for reducing microorganisms. Sanitizing with chlorine dioxide at a concentration of 200 mg/L during 10 minutes is indicated to ensure a safe product. © 2015 Sociedade de Olericultura do Brasil. All rights reserved.


PubMed | Polo Regional Centro Oeste
Type: Journal Article | Journal: The journal of venomous animals and toxins including tropical diseases | Year: 2014

This study aimed to evaluate the occurrence of Leishmania spp. in dogs and cats from Botucatu, So Paulo state, and Campo Grande, Mato Grosso do Sul state, Brazil, by the association of three diagnostic tests: blood culture in liver infusion tryptose medium, immunofluorescent antibody test and polymerase chain reaction. Fifty blood samples of dogs and cats from the Center for Zoonosis Control in Campo Grande, an area endemic for canine visceral leishmaniasis, were collected randomly, as well as canine and feline blood samples from the Municipal Kennel and Animal Protection Association in Botucatu, currently considered a transmission-free, non-endemic area.Of the 50 dog blood cultures from Botucatu, three (6%) were positive and of the 50 cats, two (4%) were positive. In Campo Grande, 29 dog blood cultures (58%) were positive and all (100%) cats negative by this test. Polymerase chain reaction detected Leishmania spp. in 100% of dog and cat samples from Botucatu but found all the cats from Campo Grande to be negative. On the other hand, 36 dogs from Campo Grande were positive (72%) by the same technique. Immunofluorescent antibody test in Botucatu found 100% of dogs and cats non-reactive, while in Campo Grande, it detected positivity in 32 dogs (64%) and 15 cats (30%).The results show the importance of not only continuous epidemiological surveillance in areas not endemic for leishmaniasis, but also research for accurate diagnosis of this zoonosis.

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