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Novi Sad, Serbia

Background/Aim. Prolonged exposure to hyperbaric oxygen leads to changes of erythrocytes shape as a consequence of toxic effects of oxygen on the erythrocyte membrane. The aim of this study was to examine the association between occurance of pathological forms of erythrocytes at different time from the start of hyperbaric oxygenation and the moment of convulsions occurrence, an interrelationship of different pathological forms of erythrocytes during exposure to hyperbaric oxygenation, as well as the correlation between the presence of ruptured erythrocytes and function of central nervous system (CNS) after completion of hyper-baric treatment. Methods. Sixty laboratory mice, Mus mus-culus, were exposed to the wholly-oxygen pressure of 3.5 absolute atmospheres (ATA). Blood was collected at the 32nd, 34th, 36th, 38th and 40th minutes after the exposure to oxygen. Pathological forms of erythrocytes were examined by electron microscopy. A moment of convulsions occurrence was registered in all animals. After decompression neurological examinations of experimental animals were perfomed. The Pearson's coefficient of correlation, and linear regression equations for the parameters outlined in the aim of the study were calculated. Results. Hyperbaric oxy- Correspondence to: Branislava Belic ́, Skupština AP Vojvodine, Vladike Platona bb, 21 000 Novi Sad. Tel.: +381 21 4874 113, +381 63 66 91 25, Fax: +381 21 456 755. E-mail: bbelic@skupstinavojvodine.gov.rs gen caused damages of erythrocytes at the 34th minute after beginning of the treatment. Various forms of abnormal red blood cells occured, and immediately before the occurrence of irreversible changes (erythrocyte membrane rupture) echinocyte shape was dominated. A significant correlation between the number of damaged red blood cells at 34th minute and their number at the 36th, 38th and 40th minute was found. Convulsions were diagnosed significantly earlier in mice with a greater number of damaged red blood cells (p < 0.01). There was a negative correlation between the number of irreversiblly damaged red blood cells (ruptured) at the 40th minute and neurological score in the studied animals (p < 0.05). Conclusion. The analysis of altered erythrocytes during hyperbaric oxygenation could predict a moment of seizures occurrence, and therefore the duration of the therapy with hyperbaric oxygen. Ehinocytes indicate impending rupture of red blood cells and a possible occurrence of seizures. An increased number of ruptured red blood cells may also even indicate the potential burden of CNS after cessation of hyperbaric oxygenation. Source


Gantner V.,Poljoprivredni fakultet | Potocnik K.,University of Ljubljana | Kuterovac K.,Agrokor d.d. | Gantner R.,Poljoprivredni fakultet | Antunovic B.,Poljoprivredni fakultet
Mljekarstvo | Year: 2010

The aim of this research was to define methods for early prediction (based on I. milk control record) of lactation flow in Holstein heifers as well as to choose optimal one in terms of prediction fit and application simplicity. Total of 304,569 daily yield records automatically recorded on a 1,136 first lactation Holstein cows, from March 2003 till August 2008., were included in analysis. According to the test date, calving date, the age at first calving, lactation stage when I. milk control occurred and to the average milk yield in first 25th, T1 (and 25th-45th, T 2) lactation days, measuring month-calving month-age-production-time- period subgroups were formed. The parameters of analysed nonlinear and linear methods were estimated for each defined subgroup. As models evaluation measures, adjusted coefficient of determination, and average and standard deviation of error were used. Considering obtained results, in terms of total variance explanation (R2adj), the nonlinear Wood's method showed superiority above the linear ones (Wilmink's, Ali-Schaeffer's and Guo-Swalve's method) in both time-period subgroups (T1 - 97.5 % of explained variability; T2 - 98.1 % of explained vari-ability). Regarding the evaluation measures based on prediction error amount (eavg ±eSD), the lowest average error of daily milk yield prediction (less than 0.005 kg/day), as well as of lactation milk yield prediction (less than 50 kg/lactation (T1 time-period subgroup) and less than 30 kg/lactation (T2 time-period subgroup)); were determined when Wood's nonlinear prediction method were applied. Obtained results indicate that estimated Wood's regression parameters could be used in routine work for early prediction of Holstein heifer's lactation flow. Source


Belic B.,Poljoprivredni fakultet | Cincovic M.R.,Poljoprivredni fakultet | Popovic-Vranjes A.,Poljoprivredni fakultet | Pejanovic R.,Poljoprivredni fakultet | Krajinovic M.,Poljoprivredni fakultet
Mljekarstvo | Year: 2011

Heat stress is a major economic problem in dairy cattle because it leads to reduced milk production and quality. Reduced milk production and quality is the result of reduced feed intake and changes in post-absorptive metabolism of nutrients. The aim of this study was to investigate the post-absorptive use of glucose, non-esterified fatty acids (NEFA), betahydroxybutyrate (BHB) and urea in milk production by determination of postprandial concentration of metabolites and the degree of metabolites extraction in milk glands. The use of glucose for energy production was increased during heat stress, and a small amount of glucose was transported to the mammary gland. Therefore, it decreased concentration of lactose in milk. The uptake of NEFA and BHB in mammary gland was significantly greater during heat stress, due to adaptation to decreased supply of glucose. This adaptation has shown a negative impact on the percentage of milk fat and protein. Elevated concentration of urea is the result of heat stress; it easily passes through the mammary gland and shows a negative impact on milk proteins. All these changes show a negative effect on the amount of milk produced during heat stress. Reduced influx of glucose in the mammary gland, increased utilization of NEFA and BHB in milk production and increased concentrations of urea during heat stress directly affect the production and quality of milk. Source

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