Police of the Czech Republic

dubna, Czech Republic

Police of the Czech Republic

dubna, Czech Republic
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Stana I.,Police of the Czech Republic | Tokar S.,Brno University of Technology | Bucsuhazy K.,Brno University of Technology | Bilik M.,Brno University of Technology
Procedia Engineering | Year: 2017

Documentation of the traffic accidents scene is one of the main assumptions and input facts for subsequent accident process analysis. This contribution points out major deficiencies (especially lower accuracy and efficiency) of currently used conventional methods (triangular method, rectangular measurement) and means (surveying wheel, steel tapeline) for the documentation of serious accidents scenes. The article presents an advanced method of localization and traffic accidents scene documentation using geodetic total station (or GNSS) and photographic documentation of traces with selected points for subsequent rectification, possibly using an image from UAV. Indisputable advantages (particularly significant time savings), significantly more accurate traffic accidents scenes documentation results for police in the Czech Republic are evident from performed comparison of these methods at the real road accidents. © 2017 The Authors.


Vaisocherova-Lisalova H.,Academy of Sciences of the Czech Republic | Surman F.,Czech Institute of Macromolecular Chemical | Visova I.,Academy of Sciences of the Czech Republic | Vala M.,Academy of Sciences of the Czech Republic | And 9 more authors.
Analytical Chemistry | Year: 2016

Functional polymer coatings that combine the ability to resist nonspecific fouling from complex media with high biorecognition element (BRE) immobilization capacity represent an emerging class of new functional materials for a number of bioanalytical and biosensor technologies for medical diagnostics, security, and food safety. Here, we report on a random copolymer brush surface - poly(CBMAA-ran-HPMAA) - providing high BRE immobilization capacity while simultaneously exhibiting ultralow-fouling behavior in complex food media. We demonstrate that both the functionalization and fouling resistance capabilities of such copolymer brushes can be tuned by changing the surface contents of the two monomer units: nonionic N-(2-hydroxypropyl) methacrylamide (HPMAA) and carboxy-functional zwitterionic carboxybetaine methacrylamide (CBMAA). It is demonstrated that the resistance to fouling decreases with the surface content of CBMAA; poly(CBMAA-ran-HPMAA) brushes with CBMAA molar content up to 15 mol % maintain excellent resistance to fouling from a variety of homogenized foods (hamburger, cucumber, milk, and lettuce) even after covalent attachment of BREs to carboxy groups of CBMAA. The poly(CBMAA 15 mol %-ran-HPMAA) brushes functionalized with antibodies are demonstrated to exhibit fouling resistance from food samples by up to 3 orders of magnitude better when compared with the widely used low-fouling carboxy-functional oligo(ethylene glycol) (OEG)-based alkanethiolate self-assembled monolayers (AT SAMs) and, furthermore, by up to 2 orders of magnitude better when compared with the most successful ultralow-fouling biorecognition coatings - poly(carboxybetaine acrylamide), poly(CBAA). When model SPR detections of food-borne bacterial pathogens in homogenized foods are used, it is also demonstrated that the antibody-functionalized poly(CBMAA 15 mol %-ran-HPMAA) brush exhibits superior biorecognition properties over the poly(CBAA). © 2016 American Chemical Society.


Kucera J.,Research Center Rez Ltd | Kofronova K.,Police of the Czech Republic
Journal of Radioanalytical and Nuclear Chemistry | Year: 2011

Autopsy of 29-year old woman suspicious of committing suicide by the ingestion of As2O3 yielded contradictory findings. All pathological findings as well as clinical symptoms suggested acute poisoning, while a highly elevated As level of 26.4 μg g-1 in her hair collected at the autopsy, which was determined with inductively coupled plasma mass spectrometry indicated chronic poisoning. To elucidate this discrepancy, instrumental neutron activation analysis (INAA) with proven accuracy was performed of another set of sectioned hair samples. Levels of As found by INAA in the range of 0.16-0.26 μg g-1 excluded chronic poisoning, because the person died after approximately 14 h after the As2O 3 ingestion. Two reasons for the discordant As results obtained by ICP-MS and INAA are considered: (1) accidental, non-removed contamination of hair on the As2O3 ingestion; (2) erroneous performance of ICP-MS. © 2010 Akadémiai Kiadó, Budapest, Hungary.


Michalek L.,VSB - Technical University of Ostrava | Grega M.,AGH University of Science and Technology | Leszczuk M.,AGH University of Science and Technology | Bryk D.,AGH University of Science and Technology | And 3 more authors.
Communications in Computer and Information Science | Year: 2014

The INACT tool is designed to be used by police forces in cases of prosecution for the production, distribution, and possession of child pornography (CP). The INACT tool is being developed under the INDECT research project. We performed a statistical assessment of results in order to determine success and performance in the process of searching. We focused on the size of the confidence interval, which is based on the values of first hit times. The aim of the experiments was to confirm that the INACT application is functional and performs well enough to be used by police forces in searching for CP. The paper also describes progress in the development of the INACT application with respect to the indexing and searching of movies. © Springer International Publishing Switzerland 2014.


Vaisocherova-Lisalova H.,Academy of Sciences of the Czech Republic | Visova I.,Academy of Sciences of the Czech Republic | Ermini M.L.,Academy of Sciences of the Czech Republic | Springer T.,Academy of Sciences of the Czech Republic | And 6 more authors.
Biosensors and Bioelectronics | Year: 2016

Recent outbreaks of foodborne illnesses have shown that foodborne bacterial pathogens present a significant threat to public health, resulting in an increased need for technologies capable of fast and reliable screening of food commodities. The optimal method of pathogen detection in foods should: (i) be rapid, specific, and sensitive; (ii) require minimum sample preparation; and (iii) be robust and cost-effective, thus enabling use in the field. Here we report the use of a SPR biosensor based on ultra-low fouling and functionalizable poly(carboxybetaine acrylamide) (pCBAA) brushes for the rapid and sensitive detection of bacterial pathogens in crude food samples utilizing a three-step detection assay. We studied both the surface resistance to fouling and the functional capabilities of these brushes with respect to each step of the assay, namely: (I) incubation of the sensor with crude food samples, resulting in the capture of bacteria by antibodies immobilized to the pCBAA coating, (II) binding of secondary biotinylated antibody (Ab2) to previously captured bacteria, and (III) binding of streptavidin-coated gold nanoparticles to the biotinylated Ab2 in order to enhance the sensor response. We also investigated the effects of the brush thickness on the biorecognition capabilities of the gold-grafted functionalized pCBAA coatings. We demonstrate that pCBAA-compared to standard low-fouling OEG-based alkanethiolate self-assemabled monolayers-exhibits superior surface resistance regarding both fouling from complex food samples as well as the non-specific binding of S-AuNPs. We further demonstrate that a SPR biosensor based on a pCBAA brush with a thickness as low as 20nm was capable of detecting E. coli O157:H7 and Salmonella sp. in complex hamburger and cucumber samples with extraordinary sensitivity and specificity. The limits of detection for the two bacteria in cucumber and hamburger extracts were determined to be 57CFU/mL and 17CFU/mL for E. coli and 7.4×103CFU/mL and 11.7×103CFU/mL for Salmonella sp., respectively. In addition, we demonstrate the simultaneous detection of E. coli and Salmonella sp. in hamburger sample using a multichannel SPR biosensor having appropriate functional coatings. © 2016 Elsevier B.V.


PubMed | Academy of Sciences of the Czech Republic and Police of the Czech Republic
Type: | Journal: Biosensors & bioelectronics | Year: 2016

Recent outbreaks of foodborne illnesses have shown that foodborne bacterial pathogens present a significant threat to public health, resulting in an increased need for technologies capable of fast and reliable screening of food commodities. The optimal method of pathogen detection in foods should: (i) be rapid, specific, and sensitive; (ii) require minimum sample preparation; and (iii) be robust and cost-effective, thus enabling use in the field. Here we report the use of a SPR biosensor based on ultra-low fouling and functionalizable poly(carboxybetaine acrylamide) (pCBAA) brushes for the rapid and sensitive detection of bacterial pathogens in crude food samples utilizing a three-step detection assay. We studied both the surface resistance to fouling and the functional capabilities of these brushes with respect to each step of the assay, namely: (I) incubation of the sensor with crude food samples, resulting in the capture of bacteria by antibodies immobilized to the pCBAA coating, (II) binding of secondary biotinylated antibody (Ab2) to previously captured bacteria, and (III) binding of streptavidin-coated gold nanoparticles to the biotinylated Ab2 in order to enhance the sensor response. We also investigated the effects of the brush thickness on the biorecognition capabilities of the gold-grafted functionalized pCBAA coatings. We demonstrate that pCBAA-compared to standard low-fouling OEG-based alkanethiolate self-assemabled monolayers-exhibits superior surface resistance regarding both fouling from complex food samples as well as the non-specific binding of S-AuNPs. We further demonstrate that a SPR biosensor based on a pCBAA brush with a thickness as low as 20 nm was capable of detecting E. coli O157:H7 and Salmonella sp. in complex hamburger and cucumber samples with extraordinary sensitivity and specificity. The limits of detection for the two bacteria in cucumber and hamburger extracts were determined to be 57 CFU/mL and 17 CFU/mL for E. coli and 7.4 10(3) CFU/mL and 11.7 10(3)CFU/mL for Salmonella sp., respectively. In addition, we demonstrate the simultaneous detection of E. coli and Salmonella sp. in hamburger sample using a multichannel SPR biosensor having appropriate functional coatings.

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