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Botana-Rial M.,Pneumology Service Of Complexo Hospitalario Universitario Of Vigo | Leiro-Fernandez V.,Pneumology Service Of Complexo Hospitalario Universitario Of Vigo | Represas-Represas C.,Pneumology Service Of Complexo Hospitalario Universitario Of Vigo | Del Campo-Perez V.,Complexo Hospitalario Universitario Of Vigo | Fernandez-Villar A.,Pneumology Service Of Complexo Hospitalario Universitario Of Vigo
Cancer Biology and Therapy | Year: 2012

Lung adenocarcinoma is one of the most frequent causes of malignant pleural effusions (MPE ). The presence of MPE bears a poor prognosis. Although epigenetic changes are commonly related to human neoplasia, scarce date is available on patients with MPE . We aimed to estimate the prognostic value of DNA methylation of tumor suppressor genes from pleural fluid. Thirty patients with MPE due to lung adenocarcinoma were prospectively included. Methylation-specific (MS) PCR was used to study the methylation status of the promoter region of tumor suppressor genes p16/INK4a, MGMT, BRCA1 and RARβ in pleural fluid. Clinical data and survival were collected. Survival analysis was performed using Kaplan-Meier plots and Cox regression. Hypermethylation in at least one gene was detected in 25 patients (83.3%). On multivariate analysis factors significantly associated with shorter survival were the lack of hypermethylation in any of the studied genes (hazard ratio = 9.3; p = 0.001), Charlson index ≥ 3 (hazard ratio = 9.6, p = 0.002) and no oncological treatment (hazard ratio = 11.1; p < 0.001). Analysis of aberrant promoter hypermethylation of tumor suppressor genes may be useful in predicting prognosis, but further studies are needed to validate our findings. © 2012 Landes Bioscience.

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