Pleinlaan

Brussels, Belgium

Pleinlaan

Brussels, Belgium
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Rousseau S.,Parenting and Special Education Research Unit | Grietens H.,Center for Special Needs Education and Youth Care | Vanderfaeillie J.,Pleinlaan | Hoppenbrouwers K.,Center for Youth Health Care | Van Leeuwen K.,Parenting and Special Education Research Unit
Journal of Psychosomatic Research | Year: 2014

Objective: The impact of somatisation in adolescence is substantial. Knowledge on (predictors of) individual-level development of somatisation is necessary to develop tailored treatment. The current study assessed individual-level development of somatisation by means of latent mixed modelling. Parenting stress was included as a predictor of somatisation trajectory membership and within-trajectory variation. Methods: A total of 1499 adolescents and one of their parents (mostly the mother) agreed to participate. Questionnaires were administered when the adolescents were respectively 12-13 (T1), 13-14 (T2), and 14-15 (T3) years old. Adolescents reported on their somatisation, parents on their parenting stress. Results: Four individual somatisation trajectories were found: increased, long-term low, long-term high, and decreased. Higher early parenting stress (T1) significantly predicted less favourable trajectory membership (increased and long-term high). The relation between later parenting stress (T2 and T3) and somatisation depended on trajectory membership. For adolescents in the long-term high and decreased somatisation trajectories, lower T2 and T3 parenting stress was related to higher somatisation, while for adolescents in the long-term low and increased trajectories, higher T2 and T3 parenting stress was related to higher somatisation. Conclusions: The results support a general recommendation to prevent the onset of high levels of parenting stress. In addition, for families in which high levels of parenting stress already exist, clinicians should be aware of natural fluctuations in parenting stress, its associated features (e.g., aspects of overall care, like looking for professional help) and of the consequences this might have for the adolescent. © 2014 Elsevier Inc.


PubMed | Center for Special Needs Education and Youth Care, Pleinlaan, Parenting and Special Education Research Unit and Center for Youth Health Care
Type: Journal Article | Journal: Journal of psychosomatic research | Year: 2014

The impact of somatisation in adolescence is substantial. Knowledge on (predictors of) individual-level development of somatisation is necessary to develop tailored treatment. The current study assessed individual-level development of somatisation by means of latent mixed modelling. Parenting stress was included as a predictor of somatisation trajectory membership and within-trajectory variation.A total of 1499 adolescents and one of their parents (mostly the mother) agreed to participate. Questionnaires were administered when the adolescents were respectively 12-13 (T1), 13-14 (T2), and 14-15 (T3) years old. Adolescents reported on their somatisation, parents on their parenting stress.Four individual somatisation trajectories were found: increased, long-term low, long-term high, and decreased. Higher early parenting stress (T1) significantly predicted less favourable trajectory membership (increased and long-term high). The relation between later parenting stress (T2 and T3) and somatisation depended on trajectory membership. For adolescents in the long-term high and decreased somatisation trajectories, lower T2 and T3 parenting stress was related to higher somatisation, while for adolescents in the long-term low and increased trajectories, higher T2 and T3 parenting stress was related to higher somatisation.The results support a general recommendation to prevent the onset of high levels of parenting stress. In addition, for families in which high levels of parenting stress already exist, clinicians should be aware of natural fluctuations in parenting stress, its associated features (e.g., aspects of overall care, like looking for professional help) and of the consequences this might have for the adolescent.


Sohier J.S.,University of Liège | Laurent C.,University of Liège | Chevigne A.,CRP Sante | Pardon E.,Pleinlaan | And 8 more authors.
Biochemical Journal | Year: 2013

MβL (metallo-β-lactamase) enzymes are usually produced by multi-resistant Gram-negative bacterial strains and have spread worldwide. An approach on the basis of phage display was used to select single-domain antibody fragments (VHHs, also called nanobodies) that would inhibit the clinically relevant VIM (Verona integron-encoded MβL)-4 MβL. Out of more than 50 selected nanobodies, only the NbVIM-38 nanobody inhibited VIM-4. The paratope, inhibition mechanism and epitope of the NbVIM-38 nanobody were then characterized. An alanine scan of the NbVIM-38 paratope showed that its binding was driven by hydrophobic amino acids. The inhibitory concentration was in the micromolar range for all β-lactams tested. In addition, the inhibition was found to follow a mixed hyperbolic profile with a predominantly uncompetitive component. Moreover, substrate inhibition was recorded only after nanobody binding. These kinetic data are indicative of a binding site that is distant from the active site. This finding was confirmed by epitope mapping analysis that was performed using peptides, and which identified two stretches of amino acids in the L6 loop and at the end of the α2 helix. Because this binding site is distant from the active site and alters both the substrate binding and catalytic properties of VIM-4, this nanobody can be considered as an allosteric inhibitor. © The Authors.


Fislage M.,Pleinlaan | Fislage M.,Vrije Universiteit Brussel | Roovers M.,Institute Of Recherches Microbiologiques Wiame | Munnich S.,Pleinlaan | And 4 more authors.
Acta Crystallographica Section F: Structural Biology and Crystallization Communications | Year: 2011

Methyltransferases form a major class of tRNA-modifying enzymes that are needed for the proper functioning of tRNA. Here, the expression, purification and crystallization of two related putative tRNA methyltransferases from two kingdoms of life are reported. The protein encoded by the gene pf1002 from the archaeon Pyrococcus furiosus was crystallized in the monoclinic space group P21. A complete data set was collected to 2.2 Å resolution. The protein encoded by the gene ttc1157 from the eubacterium Thermus thermophilus was crystallized in the trigonal space group P3221. A complete data set was collected to 2.05 Å resolution. © 2011 International Union of Crystallography. All rights reserved.


Thienpont H.,Pleinlaan | Van Erps J.,Pleinlaan
Optics InfoBase Conference Papers | Year: 2014

We present our polymer micro-optics technology supply chain and its key constituents. We show how it is a key-enabler for frontier applied research and demonstrate how it paves the way towards efficient technology take-up and effective industrial innovation. © 2014 OSA.


Cornelis B.,Pleinlaan
Electronic Letters on Computer Vision and Image Analysis | Year: 2015

Computer processing of digital images of paintings has become a fast growing and challenging field of research during the last few years. Our contribution to this research field consists of a set of tools that are based on di- mensionality reduction, sparse representations and dictionary learning. These tools are used to assist in art related matters such as restoration, conservation, art history, material and structure characterization, authenti- cation, dating and even style analysis. Since paintings are complex structures the analysis of all pictorial layers and their support requires a multimodal set of high-resolution image acquisitions.


Barbe K.,Pleinlaan | Van Moer W.,Pleinlaan
MeMeA 2012 - 2012 IEEE Symposium on Medical Measurements and Applications, Proceedings | Year: 2012

Oscillometric blood pressure devices are popular and are considered part of the family's medical chest at home. The popularity of these devices for private use is not shared by physicians mainly due to the fact that the blood pressures are computed instead of measured. The classical way to compute the systolic and diastolic blood pressure is based on the envelope of the oscillometric waveform. The algorithm to compute the blood pressures from the waveform is firm dependent, often patented and lacks scientific foundation. In this paper, we propose a totally new approach. Instead of the envelope of the oscillometric waveform, we use a statistical test to pin-point the time instances where the systolic and diastolic blood pressures are measured in the cuff. This technique has the advantage of being mathematically well-posed instead of the ill-posed problem of envelope fitting. Hence, in order to calibrate the oscillometric blood pressure monitor it is sufficient to make the statistical test unbiased. © 2012 IEEE.


PubMed | Pleinlaan
Type: Journal Article | Journal: Acta crystallographica. Section F, Structural biology and crystallization communications | Year: 2011

Methyltransferases form a major class of tRNA-modifying enzymes that are needed for the proper functioning of tRNA. Here, the expression, purification and crystallization of two related putative tRNA methyltransferases from two kingdoms of life are reported. The protein encoded by the gene pf1002 from the archaeon Pyrococcus furiosus was crystallized in the monoclinic space group P2(1). A complete data set was collected to 2.2 resolution. The protein encoded by the gene ttc1157 from the eubacterium Thermus thermophilus was crystallized in the trigonal space group P3(2)21. A complete data set was collected to 2.05 resolution.

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