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Dreuil-lès-Amiens, France

Renouard S.,CNRS Laboratory of Woody Plants and Crops Biology | Tribalatc M.-A.,Laboratoire BIOPI EA 3900 | Lamblin F.,CNRS Laboratory of Woody Plants and Crops Biology | Mongelard G.,University of Picardie Jules Verne | And 9 more authors.
Journal of Plant Physiology | Year: 2014

RNAi technology was applied to down regulate LuPLR1 gene expression in flax (Linum usitatissimum L.) seeds. This gene encodes a pinoresinol lariciresinol reductase responsible for the synthesis of (+)-secoisolariciresinol diglucoside (SDG), the major lignan accumulated in the seed coat. If flax lignans biological properties and health benefits are well documented their roles in planta remain unclear. This loss of function strategy was developed to better understand the implication of the PLR1 enzyme in the lignan biosynthetic pathway and to provide new insights on the functions of these compounds. RNAi plants generated exhibited LuPLR1 gene silencing as demonstrated by quantitative RT-PCR experiments and the failed to accumulate SDG. The accumulation of pinoresinol the substrate of the PLR1 enzyme under its diglucosylated form (PDG) was increased in transgenic seeds but did not compensate the overall loss of SDG. The monolignol flux was also deviated through the synthesis of 8-5' linked neolignans dehydrodiconiferyl alcohol glucoside (DCG) and dihydro-dehydrodiconiferyl alcohol glucoside (DDCG) which were observed for the first time in flax seeds. © 2014 Elsevier GmbH.

Fontaine J.-X.,EA 3900 BioPI | Fontaine J.-X.,Universite Ibn Tofail | Molinie R.,EA 3900 BioPI | Terce-Laforgue T.,French National Institute for Agricultural Research | And 4 more authors.
Comptes Rendus Chimie | Year: 2010

It is now well established that the GS/GOGAT cycle is the major route for ammonium assimilation in higher plants. However, it has often been argued that other enzymes, such as glutamate dehydrogenase, have the capacity to assimilate ammonium, leading to the hypothesis that alternative ammonium assimilatory pathways could operate under particular physiological conditions. The GDH enzyme is encoded by two distinct genes, GDH1 and GDH2. A third gene, GDH3, potentially encoding GDH has recently been identified by in silico studies performed on Arabidopsis thaliana. In order to precise its function, the metabolic profile of gdh3 knock out mutants were compared to wild type plants using the 1H-NMR technique. 1H-NMR spectra coupled with principal component analysis and partial least square-discriminant analysis were applied to identify changes of the metabolic profiles. These experiments were performed on roots, leaves and stems. In the gdh3 mutant, metabolic variations were observed for carboxylic acids, amino acids and carbohydrates content. © 2009 Académie des sciences.

Sarazin V.,French National Center for Scientific Research | Duclercq J.,French National Center for Scientific Research | Mendou B.,French National Center for Scientific Research | Aubanelle L.,French National Center for Scientific Research | And 6 more authors.
Plant Science | Year: 2015

During their life cycle, plants have to cope with fluctuating environmental conditions. The perception of the stressful environmental conditions induces a specific stress hormone signature specifying a proper response with an efficient fitness. By reverse genetics, we isolated and characterized a novel mutation in Arabidopsis, associated with environmental stress responses, that affects the At5g11250/BURNOUT1 (BNT1) gene which encode a Toll/Interleukin1 receptor-nucleotide binding site leucine-rich repeat (TIR-NBS-LRR) protein. The knock-out bnt1 mutants displayed, in the absence of stress conditions, a multitude of growth and development defects, suchas severe dwarfism, early senescence and flower sterility, similar to those observed in vitro in wild type plants upon different biotic and/or abiotic stresses. The disruption of BNT1 causes also a drastic increase of the jasmonic, salicylic and abscisic acids as well as ethylene levels. Which was consistent with the expression pattern observed in bnt1 showing an over representation of genes involved in the hormonal response to stress? Therefore, a defect in BNT1 forced the plant to engage in an exhausting general stress response, which produced frail, weakened and poorly adapted plants expressing "burnout" syndromes. Furthermore, by in vitro phenocopying experiments, physiological, chemical and molecular analyses, we propose that BNT1 could represent a molecular link between stress perception and specific hormonal signature. © 2015 Elsevier Ireland Ltd.

Fliniaux O.,University of Picardie Jules Verne | Gaillard G.,Biobanque de Picardie | Lion A.,University of Picardie Jules Verne | Cailleu D.,Plateforme analytique | And 2 more authors.
Journal of Biomolecular NMR | Year: 2011

A blood pre-centrifugation delay of 24 h at room temperature influenced the proton NMR spectroscopic profiles of human serum. A blood pre-centrifugation delay of 24 h at 4°C did not influence the spectroscopic profile as compared with 4 h delays at either room temperature or 4°C. Five or ten serum freeze-thaw cycles also influenced the proton NMR spectroscopic profiles. Certain common in vitro preanalytical variations occurring in biobanks may impact the metabolic profile of human serum. © 2011 Springer Science+Business Media B.V.

Broyart C.,EA 3900 BioPI Biologie des Plantes et Controle des Insectes Ravageurs | Broyart C.,Universite Ibn Tofail | Broyart C.,French National Institute for Agricultural Research | Fontaine J.-X.,EA 3900 BioPI Biologie des Plantes et Controle des Insectes Ravageurs | And 10 more authors.
Phytochemical Analysis | Year: 2010

Introduction - Maize mutants deficient for the expression of two genes encoding cytosolic glutamine syntehtase (GS) isoenzymes GS1.3 and GS1.4 displayed reduced kernel number and kernel size, respectively, the effect of the mutation being cumulative in the double mutant. However, at maturity, shoot biomass production was not modified in all the mutants, indicating that the reaction catalysed by the enzyme is specifically involved in the control of grain yield. Objective - To examine the physiological impact of the GS mutations on the leaf metabolic profile during the kernel filling period, during which nitrogen is remobilised from the shoots to be further exported to the kernels. Methodology - An 1H-NMR spectroscopy metabolomic was applied to the investigation of metabolic change of the gln1.3, gln1.4 and gln1.3/1.4 double mutant. Results - In the three GS mutants, an increase in the amount of several N-containing metabolites such as asparagine, alanine, threonine and phophatidylcholine was observed whatever the level of nitrogen fertilisation. In addition, we found an accumulation of phenylalanine and tyrosine, two metabolites involved the primary steps of the phenylpropanoid pathway. Conclusion - Changes in the metabolic profile of the GS mutants suggest that, when cytosolic GS activity is strongly reduced, either alternative metabolic pathways participate in the reassimilation of ammonium released during leaf protein remobilisation or that premature leaf senescence is induced when kernel set and kernel filling are affected. The accumulation of phenylalanine and tyrosine in the mutant plants indicates that lignin biosynthesis is altered, thus possibly affecting ear development. Copyright © 2009 John Wiley & Sons, Ltd.

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