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RANCO VA, Italy

Grant
Agency: Cordis | Branch: FP7 | Program: CP-FP | Phase: HEALTH.2011.1.1-1 | Award Amount: 6.38M | Year: 2012

Proteins are extremely malleable building blocks of life involved in all aspects of biology. Many diseases are caused by proteins aberrations, and proteins are frequent targets of intervention. Mapping all proteins and their functions are expected to yield pervasive medical and biotechnological benefits. However, even the most comprehensive and high-throughput proteins discovery technologies are seriously challenged by the extreme diversity and low abundance of many proteome components; a problem, compounded by the lack of affinity reagents and validated probes for sample preparation and identification. Our concept is that shorter protein fragments, or peptides, may offer solutions to many of these problems as peptides may represent or mimic proteins. Using in situ solid-phase peptide synthesis, computerized photolithography and novel photochemistry, we have recently generated peptide microarrays of up to 2 million addressable peptides. This unprecedented high-density and high-content peptide microarray technology could make inroads into the kind of high-throughput analysis needed to address the entire human proteome. Here, we aim to exploit this potential by using and improving three different, yet complementary, label-free detection technologies allowing sensitive, high-resolution determinations of the identity, quality and/or modification of individual members of a peptide microarray, and real-time monitoring of any interacting molecular receptor. We will also develop peptides as rapid, specific, and renewable affinity reagents for complex sample preparation, and develop peptides as probes and complex biosensors. Three SMEs constitute the backbone of this collaboration, receiving 50% of the budget, and enjoying significant opportunities from the booming protein/peptide microarray market. Furthermore, solutions to these unmet needs of proteomics are believed to have incalculable benefits for European health, innovativeness and competiveness.


Grant
Agency: Cordis | Branch: FP7 | Program: MC-IAPP | Phase: FP7-PEOPLE-2011-IAPP | Award Amount: 589.91K | Year: 2011

The objective of the IMPRESS project is to develop an affordable, portable, multiplexing and flexible Surface Plasmon Resonance (SPR) biosensor device (the IMPRESSOR), based on Plasmores nanotechnology expertise, to obtain a fast impression of the quality and safety of food. Affordable: the system will be affordable for any small or medium enterprise or even private user, which is producing or distributing or consuming food in any country or region of the world. Portable: the system will be easy-to-use in any environment and must work outside any specialized laboratory. Multiplexed: the system will be able to measure the concentration of many contaminants in a single sample of food and within a single measurement. Flexible: the system is designed to develop assays according to the wishes of the future users (customized). Fast: measurements are done in minutes and the sample-to-result time will be around 30 minutes. This will allow the real time monitoring of the quality of food. This system will be constituted by two fundamental elements: 1) A disposable biochip customized for the detection of a set of parameters of the quality of the food (e.g. the detection of a set of allergens, toxins or antibiotics) and 2) an electronic reading system enabling the dispensing and the analytical screening of the food sample and the electronic evaluation, storage and communication of the results. Such a system will have a huge impact in the food monitoring protocols and will significantly contribute to the spreading of alternative, fast and reliable analytical methods for food safety control. This will help: - industries and distributors to provide safer and healthier food products, - public regulatory bodies to improve the quality of the food control tests while reducing the assay costs, - end-users and small distributors to verify the quality of their everyday food products

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