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Wageningen, Netherlands

Burema B.S.,Center for Crop Systems Analysis | Buck-Sorlin G.H.,Center for Crop Systems Analysis | Damen T.,Center for Crop Systems Analysis | Vos J.,Center for Crop Systems Analysis | And 2 more authors.
Acta Horticulturae | Year: 2010

Growing in lower planting density, rose plants produce more assimilates, which can be used to produce more and/or heavier flowering shoots. The effect of planting density was investigated during a period including the first five flowering flushes of a young crop. In a heated greenhouse two cut-rose cultivars were grown under bent canopy management. Akito on own-roots and Ilios on Natal Briar rootstock were planted with densities of 8 and 4 plants per m2. Starting at the end of June 2007, flowering shoots were harvested over a time span of eight months. Based on flowering flushes, times of high harvest rate, the harvesting time span could be divided into five consecutive periods, each including one flush. The cultivars showed contrasting responses to planting density. In the first three periods the response in Ilios was extraordinary, because at low density plants did not produce more flowering shoots, as would be expected. However, the response in shoot fresh weight was larger for Ilios than for Akito, 35% compared to 21% over the entire study period. The results imply that there was a genetic difference in the effect of assimilate availability and/or local light environment. During the first three periods, these factors can not have influenced shoot number in Ilios, while they did in Akito. It is suggested that decreases of assimilate availability in winter caused the shoot number response to emerge for Ilios later on.

Gaquerel E.,Max Planck Institute for Chemical Ecology | Kotkar H.,Max Planck Institute for Chemical Ecology | Kotkar H.,CSIR - National Chemical Laboratory | Onkokesung N.,Max Planck Institute for Chemical Ecology | And 4 more authors.
PLoS ONE | Year: 2013

In a transcriptomic screen of Manduca sexta-induced N-acyltransferases in leaves of Nicotiana attenuata, we identified an N-acyltransferase gene sharing a high similarity with the tobacco lignin-biosynthetic hydroxycinnamoyl-CoA:shikimate/quinate hydroxycinnamoyl transferase (HCT) gene whose expression is controlled by MYB8, a transcription factor that regulates the production of phenylpropanoid polyamine conjugates (phenolamides, PAs). To evaluate the involvement of this HCT-like gene in lignin production as well as the resulting crosstalk with PA metabolism during insect herbivory, we transiently silenced (by VIGs) the expression of this gene and performed non-targeted (UHPLC-ESI/TOF-MS) metabolomics analyses. In agreement with a conserved function of N. attenuata HCT-like in lignin biogenesis, HCT-silenced plants developed weak, soft stems with greatly reduced lignin contents. Metabolic profiling demonstrated large shifts (up to 12% deregulation in total extracted ions in insect-attacked leaves) due to a large diversion of activated coumaric acid units into the production of developmentally and herbivory-induced coumaroyl-containing PAs (N′,N′′-dicoumaroylspermidine, N′,N′′-coumaroylputrescine, etc) and to minor increases in the most abundant free phenolics (chlorogenic and cryptochlorogenic acids), all without altering the production of well characterized herbivory-responsive caffeoyl- and feruloyl-based putrescine and spermidine PAs. These data are consistent with a strong metabolic tension, exacerbated during herbivory, over the allocation of coumaroyl-CoA units among lignin and unusual coumaroyl-containing PAs, and rule out a role for HCT-LIKE in tuning the herbivory-induced accumulation of other PAs. Additionally, these results are consistent with a role for lignification as an induced anti-herbivore defense. © 2013 Gaquerel et al.

van Dijk A.D.J.,Applied Bioinformatics | van Dijk A.D.J.,Plant science Group | van Mourik S.,Plant science Group | van Ham R.C.H.J.,Applied Bioinformatics | van Ham R.C.H.J.,Keygene NV
PLoS ONE | Year: 2012

Mutational robustness of gene regulatory networks refers to their ability to generate constant biological output upon mutations that change network structure. Such networks contain regulatory interactions (transcription factor - target gene interactions) but often also protein-protein interactions between transcription factors. Using computational modeling, we study factors that influence robustness and we infer several network properties governing it. These include the type of mutation, i.e. whether a regulatory interaction or a protein-protein interaction is mutated, and in the case of mutation of a regulatory interaction, the sign of the interaction (activating vs. repressive). In addition, we analyze the effect of combinations of mutations and we compare networks containing monomeric with those containing dimeric transcription factors. Our results are consistent with available data on biological networks, for example based on evolutionary conservation of network features. As a novel and remarkable property, we predict that networks are more robust against mutations in monomer than in dimer transcription factors, a prediction for which analysis of conservation of DNA binding residues in monomeric vs. dimeric transcription factors provides indirect evidence. © 2012 van Dijk et al.

van der Fels-Klerx H.J.,RIKILT Institute of Food Safety | Burgers S.L.G.E.,RIKILT Institute of Food Safety | Burgers S.L.G.E.,Plant science Group | Booij C.J.H.,Plant science Group
Food Additives and Contaminants - Part A Chemistry, Analysis, Control, Exposure and Risk Assessment | Year: 2010

Predictions of deoxynivalenol (DON) content in wheat at harvest can be useful for decision-making by stakeholders of the wheat feed and food supply chain. The objective of the current research was to develop quantitative predictive models for DON in mature winter wheat in the Netherlands for two specific groups of end-users. One model was developed for use by farmers in underpinning Fusarium spp. disease management, specifically the application of fungicides around wheat flowering (model A). The second model was developed for industry and food safety authorities, and considered the entire wheat cultivation period (model B). Model development was based on observational data collected from 425 fields throughout the Netherlands between 2001 and 2008. For each field, agronomical information, climatic data and DON levels in mature wheat were collected. Using multiple regression analyses, the set of biological relevant variables that provided the highest statistical performance was selected. The two final models include the following variables: region, wheat resistance level, spraying, flowering date, several climatic variables in the different stages of wheat growing, and length of the period between flowering and harvesting (model B only). The percentages of variance accounted for were 64.4% and 65.6% for models A and B, respectively. Model validation showed high correlation between the predicted and observed DON levels. The two models may be applied by various groups of end-users to reduce DON contamination in wheat-derived feed and food products and, ultimately, reduce animal and consumer health risks. © 2010 Taylor & Francis.

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