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Raghavan R.,Presidency College at Chennai | Elumalai S.,Presidency College at Chennai | Babu K.N.,Indian Institute of Spices Research | Jayaraman P.,Plant Anatomy Research Center
Biosciences Biotechnology Research Asia | Year: 2010

Foot rot caused by phytophthora capsici is the most serious diseases of black pepper. Though all varieties of black pepper are susceptible to this pathogen, variation do exist concering the degree of tolerance and mechanisms of defense. Anatomical features such as thicker epidermis, smaller no: of epidermal appendages, smaller cortical cells, smaller stele, thicker pericycle cells and smaller vascular bundles and compact arrangement of cells can be attributed to immunity of II -SR Shakthi to phytophthora capsici as compared to kalluvally. Observed anatomical differencess between the 3 genotypes can be used for selecting parents for disease resistant breeding.

Parthasarathy M.,SASTRA University | Pemaiah B.,SASTRA University | Natesan R.,SASTRA University | Padmavathy S.R.,SASTRA University | Pachiappan J.,Plant Anatomy Research Center
Bioelectrochemistry | Year: 2015

Salt glands are specialized organelles present in the leaf tissues of halophytes, which impart salt-tolerance capability to the plant species. These glands are usually identified only by their morphology using conventional staining procedures coupled with optical microscopy. In this work, we have employed scanning electrochemical microscopy to identify the salt glands not only by their morphology but also by their salt excretion behavior. Bermuda grass (Cynodon dactylon L.) species was chosen for the study as they are known to be salt-tolerant and contain salt glands on leaf surfaces. Scanning electrochemical microscopy performed in sodium chloride medium in the presence and absence of potassium ferrocyanide as redox mediator, reveals the identity of salt glands. More insight into the ion expulsion behavior of these glands was obtained by mapping lateral and vertical variations in ion concentrations using surface impedance measurements which indicated five times higher resistance over the salt glands compared to the surrounding tissues and bulk solution. The protocol could be used to understand the developmental processes in plants grown in different soil/water conditions in order to improve salt tolerance of food crops by genetic engineering and hence improve their agricultural productivity. © 2014 Elsevier B.V.

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