Li Y.,China Agricultural University |
Li Y.,Plant Protection Institute of Hebei Academy of Agricultural and Forestry science |
Qin Y.,China Agricultural University |
Gao Z.,Plant Protection Institute of Hebei Academy of Agricultural and Forestry science |
And 3 more authors.
African Journal of Biotechnology | Year: 2012
Chemosensory proteins (CSPs) are thought to play an important role in olfactory mediating perception, identification, transportation, and transduction of semio-chemicals. These proteins are also associated with the regulation of circadian rhythms and maturation of certain tissues or appendages. In this study, a cDNA from Bemisia tabaci encoding a CSP (GU250808), denoted BtabCSP was cloned by RT-PCR and rapid amplification of cDNA ends (RACE) technique. The genomic DNA sequence comparisons revealed a 1490 bp intron flanking the full length cDNA. Sequencing and structural analyses of the full length cDNA indicated that BtabCSP is 381 bp in length, encoding 126 amino acid residues of which a 22 amino acid residue coded for a signal peptide. The predicted molecular weight of BtabCSP is 14.17 kDa. The BtabCSP amino acid residues deduced from the respective full-length cDNA shares four conserved cysteine motifs with known CSPs from other insects. Homology modelling indicated a very good fit between the structural conformation of BtabCSP and a moth CSP molecule. The results of phylogenetic analyses indicates that the CSPs gene of Hemipteran insects have two more sub-families. The recombinant BtabCSP was successfully expressed in Escherichia coli cells. This is the first report on the existence of chemosensory protein-coding gene in whiteflies. It will help us to elucidate the molecular basis of whitefly behaviour, and explore new approach for the management of this major pest. © 2012 Academic Journals.
Yan X.,Ghent University |
Yan X.,Guangdong Entomological Institute |
De Clercq P.,Ghent University |
Han R.,Guangdong Entomological Institute |
And 4 more authors.
Nematology | Year: 2011
A solution consisting of a mixture of glycerol and fortified artificial seawater was used to induce partial anhydrobiosis at 15°C in different strains of Steinernema carpocapsae. All the strains tested were tolerant to the osmotic solution and the survival and infectivity of the nematodes were not influenced. The osmotic treatment greatly improved heat tolerance of all the tested strains but the heat tolerance of S. carpocapsae MR7 was significantly poorer than that of the other strains. This method could be used to induce S. carpocapsae into partial anhydrobiosis and thereby improve storage of the nematodes. Expression patterns of stress-related genes after osmotic treatment were compared in a heat tolerant strain (All) and a more heat sensitive strain (MR7) after induction of anhydrobiosis. Differences in gene expression after induction of anhydrobiosis between strain MR7 and All were observed that may be related to differences in subsequent heat tolerance. © 2011 Koninklijke Brill NV, Leiden.