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Bayraktar H.,Ankara University | Oksal E.,Plant Protection Central Research Institute
Phytoparasitica | Year: 2011

Isolates of Pyrenochaeta lycopersici, the causal agent of corky root rot on tomato plants, were assessed for physiological and genetic characteristics using conventional and molecular techniques. All isolates were able to produce necrosis on tomato roots and classified into temperature group according to the optimal growth temperatures. Specific-PCR assays and DNA sequence analysis of the ribosomal DNA (rDNA) internal transcribed spacer region confirmed the existence of both types (Type 1 and Type 2) of the pathogen among the isolates tested. All isolates were identified as Type 2 except for isolate Pl-4, which was classified as Type 1. Restriction fragment length polymorphism (RFLP) analysis with six enzymes resulted in three distinct banding patterns among the isolates depending on the length and restriction profiles of the rDNA intergenic spacer region. Inter-simple sequence-repeat analysis revealed a high level of genetic diversity among the isolates in agreement with the data of RFLP analysis. These results indicated that there were three different intraspecific groups among Turkish isolates of P. lycopersici. The presented study is the first attempting to characterize Turkish isolates of P. lycopersici. The results obtained will be useful in screening of tomato seedlings for resistance to P. lycopersici. © 2011 Springer Science+Business Media BV.

Guler Y.,Plant Protection Central Research Institute | Dikmen F.,Hacettepe University
Journal of the Entomological Research Society | Year: 2013

Many varieties of sweet cherry are self-incompatible and the honey bee is the main pollinator used in the orchards. However, honey bee is not active in the weather temperature below 12°C or in rainy weather conditions. This study was initiated to determine the pollinator bee species of three sweet cherry orchards in Sultandaǧi reservoir (Turkey) during 2008 and 2009. Active bee species in the unfavourable weather conditions were also investigated. Malaise traps were used to collect a total of 1476 bee specimens. A total of sixty-three species belonging to Andrenidae (five species), Anthophoridae (seven species), Apidae (only one species), Halictidae (fourty-six species) and Megachilidae (four species) families were identified. It was observed that thirty-seven species were active in full bloom period of sweet cherry and thirteen of them could maintain their activities in the air temperatures below 12°C, in general. Additionally, five bee species were also active in the rainy days.

Kurbetli I.,Plant Protection Central Research Institute
Journal of Phytopathology | Year: 2013

Walnut decline caused by Phytophthora sp. occurred in an orchard in Sakarya province in Turkey. Affected young trees showed poor growth, leaf discolouration, root and crown rot and eventual death. A Phytophthora sp. isolated from necrotic taproots and crown tissues. The causal agent of the disease was identified as Phytophthora cinnamomi by morphological characteristics and comparing sequences of internal transcribed spacer (ITS) region. Upon conducting pathogenicity test, averaging 7.8-cm-long canker developed on basal stem within 2 weeks, while no cankers developed in the control plants. © 2012 Blackwell Verlag GmbH.

Ugurlu Karaagac S.,Plant Protection Central Research Institute
EPPO Bulletin | Year: 2012

Tomato is one of the most important vegetable crops in Turkey, with national production of over 10 million tonnes in 2010. Tuta absoluta (Meyrick) is an important pest of tomato, and was first recorded in Urla District of Izmir Province in the Aegean region of Turkey in August 2009. It has since spread rapidly to the other regions of Turkey and become the main pest of tomato. Since its dispersal, chemical control has been the main method of control. Intensive use of insecticides has led to the development of resistance in T. absoluta. In this study, the baseline toxicity (LC 50 values) of some insecticides was determined using a leaf-dip bioassay method in Antalya and Ankara populations of T. absoluta. © 2012 The Author. Journal compilation © 2012 OEPP/EPPO.

Some parasitic hymenopterans were reared from their host insects found on the stems and branches of Heracleum platytaenium Boiss., which contains furanocoumarins that are insect repellents and suppress growth in some species. Plant materials with insects were collected from the vicinity of Ki{dotless}zi{dotless}lcahamam in Ankara Province in July-September 2008. The parasitoids (Hymenoptera) and their hosts were Didyctium brunnea (Belizin) (Eucolidae) from puparia of Lasiambia albidipennis (Strobl) (Diptera: Chloropidae), Pronotalia carlinarum (Szelenyi & Erdos) (Eulophidae) and Homoporus febriculosus (Girault) (Pteromalidae) from puparia of Melanagromyza heracleana Zlobin (Diptera: Agromyzidae), and Baryscapus crassicornis (Erdos) (Eulophidae) from larvae, pupae, and adults of Lixus nordmanni Hochhuth (Coleoptera: Curculionidae). The hole(s) or cutting types done by the parasitoids while leaving their hosts were described. Development time from egg to adult (12 days and 19-20 days) and parasitization duration (60 min and 20-30 min) were also determined for P. carlinarum and B. crassicornis, respectively, and average parasitization rates in nature and the mating behaviors of these 2 parasitoids were defined. To our knowledge, the insects are new hosts for all parasitoids and the first parasitoid records for the flies. Chloropidae is a new record host family for D. brunnea aft er Phoridae. In addition, D. brunnea and H. febriculosus were recorded from Turkey for the first time. © TÜBİTAK.

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