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Kamenikova M.,Comenius University | Fialova S.,Comenius University | azky A.,Comenius University | Cicova I.,Plant Production Research Institute
Acta Facultatis Pharmaceuticae Universitatis Comenianae | Year: 2015

The content of phenolic compounds (total phenolic compounds, tannins, flavonoids and anthocyanidins) of three species of thyme (Thymus pulegioides, Thymus pannonicus, Thymus praecox) of different origin (Bohemian-Moravian highlands, Křivoklat and Považsky Inovec) was determined using spectrophotometric methods of European Pharmacopoeia 8th edition. Furthermore, the determination of the content of essential oil and analysis of its constituents was realised. The amount of total phenolics was determined by a spectrophotometric method using the Folin-Ciocalteu reagent. Their content ranged from 3.87 to 8.86%. The content of tannins was established on a preliminary determination of the total phenolics, followed by adsorption of tannins on hide powder. The amount of tannins in our samples varied from 1.96 to 5.65%. The content of total flavonoids was determined by a spectrophotometric method using aluminium chloride. Quantitative analysis of flavonoids has shown that content in our samples ranged from 0.59 to 1.52% expressed as luteolin-7-O-glucoside (λ = 392 nm) and from 0.41 to 1.12% expressed as rutin (λ = 420 nm). Anthocyanins represent a small amount of total polyphenol content in Thymus species. In our samples, the content of anthocyanins expressed as cyanidin-3-O-glucoside varied from 0.02 to 0.1%. The determination of essential oil was realised by method of European Pharmacopoeia 8th edition. Its contents in our samples ranged from 0.2 to 0.75%. Gas chromatography-mass spectrometry was used for essential oil analysis. According to the presence of main monoterpene in Thymus essential oil, we can distinguish three chemotypes: thymol, carvacrol and linalool. Due to variations of chemical constituents of thyme essential oil, it was possible to observe a relationship between occurrence of certain chemotype and origin of sample. Differences in content of flavonoids have not shown a significant relationship to locality of origin. © by M. Kameníková 2015. Source

Pastircakova K.,Slovak Academy of Sciences | Pastircak M.,Plant Production Research Institute
Polish Botanical Journal | Year: 2010

The paper reports two fungal species of Pleomassariaceae new for the Slovak mycobiota: Pleomassaria siparia (Berk. & Broome) Sacc. and Splanchnonema argus (Berk. & Broome) Kuntze, found on dead twigs of Betula pendula Roth. The morphology of the fungi and their coelomycetous anamorphs is described, with photographic documentation. Source

Pastircak M.,Plant Production Research Institute | Fejer J.,University of Presov
Acta Horticulturae | Year: 2014

A survey of fungal populations in discolored tissues of opium poppy (Papaver somniferum) plants was conducted throughout Slovakia in 2007-2009. More than 20 fungal taxa were isolated from opium poppy stems, leaves, capsules and seeds periodically sampled during three growing seasons. Incidence of fungi varied annually due to, in part, differences in rainfall. Symptoms of stem rot, leaf and capsule spot were observed during field surveys in commercial opium poppy. Fungal assemblages present in necrotic tissues of P. somniferum were determined at three sites in Slovakia. Fungi were identified by examination of fruiting bodies or by isolation. Of the different plant parts, the highest number of fungi were isolated from stems followed by leaves and capsules. In diseased stem tissues Crivellia papaveracea, Brachycladium penicillatum, Fusarium sp., Colletotrichum sp., and Alternaria sp. were the most common. In diseased leaf tissues Peronospora arborescens, Erysiphe cruciferarum, Botrytis cinerea, and Alternaria sp. were the most common. The ability of fungi parasitic on P. somniferum capsules was studied, with the aim of determining total mycoflora. Dominant fungi on capsule lesions were C. papaveracea, B. cinerea, Fusarium sp., and Alternaria brassicae var. somniferi. A total number of 15 fungal species belonging to 12 genera were isolated from seeds. Based on the culture phenotype, these strains were identified as Alternaria sp., Arthobotrys sp., Aspergillus sp., Botrytis sp., Epicoccum sp., Fusarium sp. (F. equiseti, F. poae), Mucor sp., Penicillium sp., Phoma sp., Rhizoctonia sp., Rhizopus sp., and Stemphylium sp. Among them, Brachycladium papaveris and Alternaria alternata had higher isolating rate than others. Source

Sramkova Z.,Slovak University of Technology in Bratislava | Gregova E.,Plant Production Research Institute | Slikova S.,Plant Production Research Institute | Sturdik E.,University of Trnava
Plant Genetic Resources: Characterisation and Utilisation | Year: 2011

The objective of our study was to determine the composition of high-molecular weight-glutenin subunits (HMW-GS) in 120 cultivars of common wheat (Triticum aestivum L.). Fourteen alleles and 34 allelic compositions were detected using sodium dodecyl sulphate-polyacrylamide gel electrophoresis. The most frequent HMW-GS alleles at the Glu-A1, Glu-B1 and Glu-D1 loci were null (57.1%), 7+9 (43.3%) and 5+10 (61.9%), respectively. However, low-frequency HMW-GS alleles were also observed, such as 13+16, 20, 21, 7 and 18, encoded by the Glu-B1 locus, and 4+12, encoded by the Glu-D1 locus. The wheat-rye 1BL.1RS translocation was identified in 25 cultivars, using acid polyacrylamide gel electrophoresis. The Glu-score varied greatly, and some lines reached the maximum value of 10. © 2011 NIAB. Source

Pastircakova K.,Slovak Academy of Sciences | Pastircak M.,Plant Production Research Institute | Adamcikova K.,Slovak Academy of Sciences | Bouznad Z.,Algeria National School of Agronomy | And 4 more authors.
Cryptogamie, Mycologie | Year: 2014

A global survey of the spread of the Platanus powdery mildew, Erysiphe platani , has been carried out. E. platani teleomorph formation was recorded in countries where the fungus anamorph has been present for several years. The first findings of chasmothecia were recorded in Austria, Czech Republic, France, Italy and Slovakia. New records of E. platani (including the teleomorph) were found in Belgium, Croatia and Denmark. The occurrence of this fungus in Sweden and in two countries of North Africa (Algeria and Morocco) was confirmed. Descriptions of morphological features, illustrations, and worldwide distribution of E. platani are provided. Herbarium collections of powdery mildews on Platanus spp. were re-examined and revised. The occurrence of Phyllactinia guttata on Platanus is discussed and questioned. © 2014 Adac. Tous droits réservés. Source

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