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Mendelova A.,Slovak University of Agriculture | Mendel L.,Plant Production Research Center Piestany | Czako P.,Slovak University of Agriculture | Marecek J.,Slovak University of Agriculture
Potravinarstvo | Year: 2016

Due to the content of biologically active substances, sea buckthorn (Hippophae rhamnoides L.) has become the object of great interest of both, experts and the general public. It is appreciated particularly for the high content of vitamins and other biologically active substances, not only in berries but also in leaves and bark. The aim of the study was to evaluate the nutritional quality of sea buckthorn juice prepared from different varieties of sea buckthorn based on the content of total carotenoids, polyphenols and antioxidant activity. In this study we used varieties Hergo, Tytti, Vitaminaja, Raisa, Askola, Dorana, Slovan, Leikora, Bojan, Terhi and Maslicnaja. Content of different components was quantified using spectrophotometry. The total carotenoids content expressed as β-carotene content in juice ranged from 50.63 mg.100 g-1 DM to 93.63 mg.100 g-1 DM, the highest content was in variety Askola and the lowest one in Terhi. Total polyphenols content determined by Folin-Ciocalteu method ranged from 13.03 mg GAE. dm-3 DM to 25.35 mg GAE. dm-3 DM. The highest content was identified in juice of variety Dorana and the lowest one in Raisa. The antioxidant activity quantified by the FOMO method ranges from 45.11 g AA. dm-3 DM to 108.77 g AA. dm-3 DM. The highest antioxidant activity was determined in juice of Dorana and the lowest in variety Bojan. © 2016 Potravinarstvo.

Benedikova D.,Plant Production Research Center Piestany | Giovannini D.,Agricultural Research Council
Acta Horticulturae | Year: 2013

The Prunus Working Group (WG) was established in 1983 as one of the six selected crop WGs developed within the European Cooperative Programme for Genetic Resources (ECPGR), a network developed to contribute to the more effective conservation and use of plant genetic resources in Europe. In preparation for the VIII Meeting of the WG, the Delegates of the member countries were asked to fill in a survey questionnaire, aimed to update information on the status of Prunus genetic resources in their countries. Answers were provided by a total of 64 European institutions (25 private, 36 public and 3 non-governmental) distributed in 20 countries. More than 22,700 Prunus accessions (mostly cultivars, varieties and landraces originated in Europe) are conserved in ex-situ collections. Most collections are coordinated and funded within the framework of a National Programme, although insufficient funds for high levels of characterization are rather common problems. There are even cases of extensive collections threatened of loss by the lack of financial support. Cherry, chosen by the WG as a model crop to develop the European Prunus DataBase, is represented by the highest number of accessions, followed by European plum, peach and apricot. Collected accessions are mostly used for breeding purposes, such as increasing fruit quality and resistance to biotic and abiotic stresses.

Mendelova A.,Slovak University of Agriculture | Fikselova M.,Slovak University of Agriculture | Mendel L.,Plant Production Research Center Piestany
Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis | Year: 2013

Important component of the tomato are carotenoid dyes, especially lycopene. The importance of lycopene in the diet of people in recent years has grown mainly for its pharmacological effects due to its ability to reduce the risk of carcinoma diseases and prevention of cardiovascular diseases. The aim of this work was to analyze the content of total carotenoids and lycopene in 8 varieties of tomato and to monitor dynamic changes after their different treatments (heating, drying). The experiment included following tomato varieties: Bambino F1, Darina F1, Diana F1, Denár, Milica F1, Orange F1, Paulína F1, Šejk F1. We found that processing of tomato fruits into juices and dried slices positively affected the presence of carotenoids and lycopene. Processing leads to an increase in the content of carotenoids that can be attributed to better availability of these components in the human body.

Matusinsky P.,Agrotest Fyto Ltd. | Leisova-Svobodova L.,Czech Republic Crop Research Institute | Gubis J.,Agrotest Fyto Ltd. | Hudcovicova M.,Plant Production Research Center Piestany | And 5 more authors.
Journal of Plant Pathology | Year: 2011

Ramularia collo-cygni (Rcc) infects spring and winter barley causing Ramularia leaf spot (RLS). During 2009 and 2010, contrasting years for the natural occurrence of RLS in the Czech Republic, infestation intensity was assessed with field experiments at two locations in relation to Ramularia contamination of seeds. A real time PCR assay was designed to quantify the pathogen in barley tissues. PCR primers and a TaqMan probe were designed to target Rcc-specific DNA sequence. The method was optimized using pure fungal DNA and plasmid standard dilutions. After washing, kernels were dissected into lemma, pericarp, testa, endosperm and embryo which were individually tested by real time PCR for quantifying Rcc, whereas the presence of other potential seed-borne pathogens was checked by standard PCR. The level of seed contamination was not the main factor influencing symptom expression. In 2009, seeds with low Rcc contamination were planted in two locations in both of which severe RLS infection had occured. In 2010, seeds with higher Rcc contamination were planted, but the extent of RLS symptoms on the leaves was much lower. Rcc occurred at higher rates in kernels from the 2009 crop, when RLS was severe. Ramularia DNA was highest in the lemma, and occurred in lower amounts in the pericarp and embryo. It was also found in the water used for washing the kernels. The results showed that Rcc does not penetrate through the testa into the endosperm.

Zofajova A.,Plant Production Research Center Piestany | Psenakova I.,University of Trnava | Psenakova I.,Constantine the Philosopher University | Havrlentova M.,Plant Production Research Center Piestany | Piliarova M.,University of Trnava
Agriculture | Year: 2012

In the recent years, for specific goals of utilization, winter wheat breeding has been aimed on increasing total anthocyanins concentration in winter wheat grains considering their high antioxidant activity. The aim of research was to evaluate grain colour development in four wheat genotypes (ANK 28A and 62/0 purple pericarp, UC 66049 blue aleurone and Ilona red pericarp) during grain filling period. Grain samples from two replications of field experiment, established in the vegetation 2010/11, were taken in five to six sampling times. Total anthocyanins concentration was determined by spectrophotometer. The genotypes responded differently to the dynamics of total anthocyanins accumulation during grain filling. The process was described by linear and also by polynomial regression on the number of days post anthesis. Genotypes with purple pericarp reached the highest total anthocyanins concentration on the 22nd day post anthesis with increasing and decreasing before and after this sampling time, respectively. At maturity the highest total anthocyanins had UC 66049 (193.38 mg/kg). Newly bred genotype 62/0 had similar concentration (34.50 mg/kg) as its parent ANK 28A (37.80 mg/kg). At maturity, registered cultivar Ilona was about 93.7% lower in total anthocyanins concentration compared to ANK 28A. Significant variability in total anthocyanins concentration indicated that breeding for their increasing is possible.

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