Benedikova D.,Plant Production Research Center Piestany |
Giovannini D.,Agricultural Research Council
Acta Horticulturae | Year: 2013
The Prunus Working Group (WG) was established in 1983 as one of the six selected crop WGs developed within the European Cooperative Programme for Genetic Resources (ECPGR), a network developed to contribute to the more effective conservation and use of plant genetic resources in Europe. In preparation for the VIII Meeting of the WG, the Delegates of the member countries were asked to fill in a survey questionnaire, aimed to update information on the status of Prunus genetic resources in their countries. Answers were provided by a total of 64 European institutions (25 private, 36 public and 3 non-governmental) distributed in 20 countries. More than 22,700 Prunus accessions (mostly cultivars, varieties and landraces originated in Europe) are conserved in ex-situ collections. Most collections are coordinated and funded within the framework of a National Programme, although insufficient funds for high levels of characterization are rather common problems. There are even cases of extensive collections threatened of loss by the lack of financial support. Cherry, chosen by the WG as a model crop to develop the European Prunus DataBase, is represented by the highest number of accessions, followed by European plum, peach and apricot. Collected accessions are mostly used for breeding purposes, such as increasing fruit quality and resistance to biotic and abiotic stresses.
PubMed | Plant Production Research Center Piestany, Czech Republic Crop Research Institute and Agrotest fyto ltd.
Type: Journal Article | Journal: Fungal biology | Year: 2014
The population structure of the fungal pathogen Pyrenophora teres, collected mainly from different regions of the Czech and Slovak Republics, was examined using a microsatellite analyses (SSR). Among 305 P. teres f. teres (PTT) and 82 P. teres f. maculata (PTM) isolates that were collected, the overall gene diversity was similar ( = 0.12 and = 0.13, respectively). A high level of genetic differentiation (FST = 0.46; P < 0.001) indicated the existence of population structure. Nine clusters that were found using a Bayesian approach represent the genetic structure of the studied P. teres populations. Two clusters consisted of PTM populations; PTT populations formed another seven clusters. An exact test of population differentiation confirmed the results that were generated by Structure. There was no difference between naturally infected populations over time, and genetic distance did not correlate with geographical distance. The facts that all individuals had unique multilocus genotypes and that the hypothesis of random mating could not be rejected in several populations or subpopulations serve as evidence that a mixed mating system plays a role in the P. teres life cycle. Despite the fact that the genetic differentiation value between PTT and PTM (FST = 0.30; P < 0.001) is lower than it is between the populations within each form (FST = 0.40 (PTT); FST = 0.35 (PTM); P < 0.001) and that individuals with mixed PTT and PTM genomes were found, the two forms of P. teres form genetically separate populations. Therefore, it can be assumed that these populations have most likely undergone speciation.
Zofajova A.,Plant Production Research Center Piestany |
Psenakova I.,University of Trnava |
Psenakova I.,Constantine the Philosopher University |
Havrlentova M.,Plant Production Research Center Piestany |
Piliarova M.,University of Trnava
Agriculture | Year: 2012
In the recent years, for specific goals of utilization, winter wheat breeding has been aimed on increasing total anthocyanins concentration in winter wheat grains considering their high antioxidant activity. The aim of research was to evaluate grain colour development in four wheat genotypes (ANK 28A and 62/0 purple pericarp, UC 66049 blue aleurone and Ilona red pericarp) during grain filling period. Grain samples from two replications of field experiment, established in the vegetation 2010/11, were taken in five to six sampling times. Total anthocyanins concentration was determined by spectrophotometer. The genotypes responded differently to the dynamics of total anthocyanins accumulation during grain filling. The process was described by linear and also by polynomial regression on the number of days post anthesis. Genotypes with purple pericarp reached the highest total anthocyanins concentration on the 22nd day post anthesis with increasing and decreasing before and after this sampling time, respectively. At maturity the highest total anthocyanins had UC 66049 (193.38 mg/kg). Newly bred genotype 62/0 had similar concentration (34.50 mg/kg) as its parent ANK 28A (37.80 mg/kg). At maturity, registered cultivar Ilona was about 93.7% lower in total anthocyanins concentration compared to ANK 28A. Significant variability in total anthocyanins concentration indicated that breeding for their increasing is possible.
Maliar T.,University of Trnava |
Drobna J.,Plant Production Research Center Piestany |
Kraic J.,University of Trnava |
Kraic J.,Plant Production Research Center Piestany |
And 2 more authors.
Biologia | Year: 2011
The hypothesis of the possible therapeutic potential of selected species of forage crops is discussed. Extracts from genotypes of Anthyllis sp., Astragalus sp., Coronilla sp., Lotus sp., Medicago sp., Melilotus sp., Onobrychis sp. and Trifolium sp. were prepared and tested for proteinase inhibition and antioxidant activities. We found that Trifolium pratense accession POLKIE99-3 expressed the highest relative trypsin inhibition activity (80.0%) compared to standards. The highest thrombin inhibition activity (81.4%) was detected in the Medicago sativa old cultivar Hodoninka, whereas the highest relative urokinase inhibition activity (62.5%) was expressed by the local population Nitranka. Relatively high antioxidant properties of Trifolium sp. accessions, Trifolium pratense genetic resources SVKZAH98-40, were of interest. Results of this study confirmed that there are significant differences in proteinase inhibition and antioxidant activity among important selected agricultural crops. The present paper may also be the starting point of the research aimed for development of new functional food and nutraceuticals, and/or of the research focused on new secondary metabolites with potential as bioactive compounds. © 2011 Versita Warsaw and Springer-Verlag Wien.
Mendelova A.,Slovak University of Agriculture |
Mendel L.,Plant Production Research Center Piestany |
Czako P.,Slovak University of Agriculture |
Marecek J.,Slovak University of Agriculture
Potravinarstvo | Year: 2016
Due to the content of biologically active substances, sea buckthorn (Hippophae rhamnoides L.) has become the object of great interest of both, experts and the general public. It is appreciated particularly for the high content of vitamins and other biologically active substances, not only in berries but also in leaves and bark. The aim of the study was to evaluate the nutritional quality of sea buckthorn juice prepared from different varieties of sea buckthorn based on the content of total carotenoids, polyphenols and antioxidant activity. In this study we used varieties Hergo, Tytti, Vitaminaja, Raisa, Askola, Dorana, Slovan, Leikora, Bojan, Terhi and Maslicnaja. Content of different components was quantified using spectrophotometry. The total carotenoids content expressed as β-carotene content in juice ranged from 50.63 mg.100 g-1 DM to 93.63 mg.100 g-1 DM, the highest content was in variety Askola and the lowest one in Terhi. Total polyphenols content determined by Folin-Ciocalteu method ranged from 13.03 mg GAE. dm-3 DM to 25.35 mg GAE. dm-3 DM. The highest content was identified in juice of variety Dorana and the lowest one in Raisa. The antioxidant activity quantified by the FOMO method ranges from 45.11 g AA. dm-3 DM to 108.77 g AA. dm-3 DM. The highest antioxidant activity was determined in juice of Dorana and the lowest in variety Bojan. © 2016 Potravinarstvo.
Slikova S.,Plant Production Research Center Piestany |
Gavurnikova S.,Plant Production Research Center Piestany |
Sudyova V.,Plant Production Research Center Piestany |
Gregova E.,Plant Production Research Center Piestany
Toxins | Year: 2013
In this study, a total of 299 grain samples of wheat were collected from four production regions: the maize, sugar beet, potato and feed sectors of Slovakia. The samples were analyzed for deoxynivalenol (DON) content by using an enzyme-linked immunosorbent assay Ridascreen® Fast DON. Analysis of variance revealed a significant difference between years in DON contents (p < 0.027). The occurrence of samples with DON was 82.2% in 2010, with maximum DON content of 7.88 mg kg-1, and 70.7% in 2011, with maximum DON content of 2.12 mg·kg-1. The total mean DON content was 0.62 mg·kg-1; in the feed region 0.22 mg·kg-1; 0.63 mg·kg-1 in the maize region; 0.78 mg·kg-1 in the sugar beet region; 0.45 mg·kg-1 the potato region. The limit of 1.25 mg·kg-1 imposed by the European Union (EU) for DON content was exceeded in 13.7% of the studied samples. The average monthly rainfall for May to June played a critical role in DON content of wheat grains for maize and sugar beet producing regions. The present results indicate that DON content was at a high level in grains from wheat grown during 2010. © 2013 by the authors; licensee MDPI, Basel, Switzerland.
Mendelova A.,Slovak University of Agriculture |
Fikselova M.,Slovak University of Agriculture |
Mendel L.,Plant Production Research Center Piestany
Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis | Year: 2013
Important component of the tomato are carotenoid dyes, especially lycopene. The importance of lycopene in the diet of people in recent years has grown mainly for its pharmacological effects due to its ability to reduce the risk of carcinoma diseases and prevention of cardiovascular diseases. The aim of this work was to analyze the content of total carotenoids and lycopene in 8 varieties of tomato and to monitor dynamic changes after their different treatments (heating, drying). The experiment included following tomato varieties: Bambino F1, Darina F1, Diana F1, Denár, Milica F1, Orange F1, Paulína F1, Šejk F1. We found that processing of tomato fruits into juices and dried slices positively affected the presence of carotenoids and lycopene. Processing leads to an increase in the content of carotenoids that can be attributed to better availability of these components in the human body.
Gubisova M.,Plant Production Research Center Piestany |
Gubisova M.,Constantine the Philosopher University |
Gubis J.,Plant Production Research Center Piestany |
Zofajova A.,Plant Production Research Center Piestany |
And 3 more authors.
Industrial Crops and Products | Year: 2013
Two approaches for Miscanthus× giganteus in vitro propagation - direct and indirect, were improved as effective alternatives to asexual propagation from rhizomes. The direct multiplication using stem segments containing axillary buds combined with in vitro tillering phase turns out to be fifty-sixty times more effective than the conventional ex vitro rhizome-based approach. On average, 64 regenerants per one immature inflorescence were produced using the indirect system through callus growth and shoots regeneration and inclusion of in vitro tillering increased multiplication by coefficient 2-2.5. Both in vitro systems were improved and could be integrated into effective production of M.× giganteus plantlets. © 2012 Elsevier B.V.
Hudcovicova M.,Plant Production Research Center piestAny |
Slikova S.,Plant Production Research Center piestAny |
Sudyova V.,Plant Production Research Center piestAny |
Hauptvogel P.,Plant Production Research Center piestAny
Agriculture | Year: 2012
Slovak winter wheat cultivars were evaluated for a level of spike and kernel infection, the content of mycotoxin deoxynivalenol (DON) and the amount of Fusarium culmorum DNA in kernels after artificial inoculation with the fungus F. culmorum (W. G. Sm.) Sacc. The tests included 14 old and 12 modern wheat Slovak cultivars. The cultivars were sown in October 2008 in field conditions of Piešt'any and inoculated with pathogen in May 2009. At anthesis, twenty-five spikes from each cultivar were sprayed (block 1) with F. culmorum and spikes were covered for 24 hours with a plastic bag. Old Slovak cultivars had lower area under the disease progress curve (AUDPC), Fusarium damaged kernels, DON content and lower amount of F. culmorum DNA than modern Slovak cultivars. An average kernel contamination with DON in the tested cultivars was 24.9 mg/kg and an average amount of F. culmorum DNA was 10,528 ng/g. The old cultivars accumulated 35.9% less DON and 51% less F. culmorum DNA than modern cultivars. The positive correlation coefficients were significant with AUDPC and DON content, and with the amount of F. culmorum DNA and DON content and AUDPC (P < 0.01). Correlation coefficients were higher when we used quantification of F. culmorum DNA expressed in infection per cent. Our study has confirmed that real-time polymerase chain reaction is very suitable method for evaluation of wheat cultivars for F. culmorum infection and presents less time-consuming, more sensitive and more specific assay than conventional assays.
PubMed | Plant Production Research Center Piestany
Type: Journal Article | Journal: Toxins | Year: 2013
In this study, a total of 299 grain samples of wheat were collected from four production regions: the maize, sugar beet, potato and feed sectors of Slovakia. The samples were analyzed for deoxynivalenol (DON) content by using an enzyme-linked immunosorbent assay Ridascreen Fast DON. Analysis of variance revealed a significant difference between years in DON contents (p < 0.027). The occurrence of samples with DON was 82.2% in 2010, with maximum DON content of 7.88 mg kg, and 70.7% in 2011, with maximum DON content of 2.12 mgkg. The total mean DON content was 0.62 mgkg; in the feed region 0.22 mgkg; 0.63 mgkg in the maize region; 0.78 mgkg in the sugar beet region; 0.45 mgkg the potato region. The limit of 1.25 mgkg imposed by the European Union (EU) for DON content was exceeded in 13.7% of the studied samples. The average monthly rainfall for May to June played a critical role in DON content of wheat grains for maize and sugar beet producing regions. The present results indicate that DON content was at a high level in grains from wheat grown during 2010.