Plant Pest Diagnostics Center

Hayward, CA, United States

Plant Pest Diagnostics Center

Hayward, CA, United States
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Garrison R.W.,Plant Pest Diagnostics Center | Von Ellenrieder N.,Plant Pest Diagnostics Center
Zootaxa | Year: 2017

Seven new species of Argia are described, five of which occur in Costa Rica: Argia calverti n. sp. (Holotype →, Costa Rica, Cartago Prov., Tapanti Reserve, 1,310 m, 6 vii 1963, F. G. Thompson leg., in FSCA); Argia carolus n. sp. (Holotype →, Costa Rica, San Jose Prov., El Rodeo Biological Reserve, 7 km W of Villa Colon, 9°54′ N, 84°16′ W, 561 m, 10-13 vii 1990, T. W. Donnelly leg., in FSCA); Argia elongata n. sp. (Holotype →, Costa Rica, Cartago Prov., Reventazon river, SE of Turrialba by highway 10, 9°52′56″ N, 83°38′49″ W, 561 m, 10 viii 1979, R. W. & J. A. Garrison leg., in CSCA); Argia haberi n. sp. (Holotype →, Costa Rica, San Jose Prov., Bosque del Tolomuco, km 118 on Pan American highway, in seeps and trickles through brushy pasture on forested hillside, 9°28′18″ N, 83°41′48″ W, 1,710 m, 27 iii 2006, F. Sibley leg., in FSCA); Argia schorri n. sp. (Holotype →, Costa Rica, Puntarenas Prov., 2.8 mi E of Golfito, 8°39′ N, 83°7′ W, 35 m, 4 vii 1967, O. S. Flint, Jr. & M. A. Ortiz B. leg., in USNM), and two which are so far only known from Mexico and Ecuador respectively: Argia rudolphi n. sp. (Holotype →, Mexico, Puebla State, Zihuateutla, Sierra de Huauchinango, La Union, in drainage area, 20°14′25″ N, 97°53′38″ W, 596 m, 21 v 1987, R. Novelo & A. Gomez leg., in CSCA) and Argia schneideri n. sp. (Holotype →, Ecuador, Napo Prov., Las Palmas, on Anzu river in Napo river watershed, 11 xii 1936, W. Clark-MacIntyre leg., in UMMZ). All the new species, as well as closely related species needed for diagnosis including A. anceps Garrison, A. cupraurea Calvert, A. cuprea (Hagen), A. extranea (Hagen), A. fissa Selys, A. fulgida Navas, A. oenea Hagen in Selys, A. popoluca Calvert, A. rhoadsi Calvert, and A. westfalli Garrison, are illustrated and diagnosed from their congeners and their known distribution areas are mapped. Copyright © 2017 Magnolia Press. All rights reserved.

Brachypeplus habecki Cline and Skelley, sp. nov. is described from southern Texas, and Brachypeplus glaber Le Conte is rediagnosed and discussed. Cyllodes thomasi Cline and Skelley, sp. nov. is described from southern Arizona, and Cyllodes biplagiatus is discussed. Habitus and genitalic photographs are provided as well as images of key diagnostic features for these species. An identification key is provided to distinguish the Brachypeplus and Cyllodes present in North America. Cryptarcha omisitoides Reitter, a previously unreported Central American nitidulid, is newly recorded from Arizona; and Carpophilus opthalmicus Murray, a previously unreported Caribbean species, is newly recorded from Florida. A discussion of Cryptarcha omisitoides and Carpophilus opthalmicus is also provided. Copyright © 2013 Magnolia Press.

Kumari S.,Czech Republic Crop Research Institute | Subbotin S.A.,Plant Pest Diagnostics Center
European Journal of Plant Pathology | Year: 2012

Longidorus helveticus was found at two out of 285 sampling sites for the first time in the Czech Republic. Females, males and juvenile stages were analyzed morphologically and morphometrically. The morphological identification of samples was verified by polymerase chain reaction using a species specific primer. Four markers of ribosomal DNA (18S, ITS1, ITS2, D2-D3 expansion segments of 28S rRNA) and two markers of mitochondrial DNA (cox1 and nad4) were sequenced and analyzed and compared with published gene sequences of other populations of L. helveticus. The partial mitochondrial cytochrome c-oxidase subunit 1 gene and partial nicotinamide dehydrogenase subunits 4 gene showed relatively high genetic variation within the species compared with ribosomal DNA markers. © 2012 KNPV.

Kumari S.,Czech Republic Crop Research Institute | Subbotin S.A.,Plant Pest Diagnostics Center | Subbotin S.A.,RAS Severtsov Institute of Ecology
Plant Pathology | Year: 2012

Plant parasitic nematodes of the family Trichodoridae cause substantial yield losses in many agricultural crops. Rapid and accurate identification of trichodorids to the species level is critical for selection of appropriate measures for control. This study analysed 99 sequences of the D2-D3 expansion segments of the 28S rRNA gene and 131 sequences of the 18S rRNA gene from the stubby nematodes belonging to the genera Nanidorus, Paratrichodorus and Trichodorus. Species delimiting was based on the integration of morphological identification, which is not provided in the present article, and molecular-based phylogenetic inference and sequence analysis. Twenty-two valid species and several species complexes were identified among nematodes included in the analysis. PCR-RFLPs of the partial 18S rDNA and the D2-D3 expansion segments of the 28S rDNA were tested and proposed for identification of these nematodes. Gel PCR-RFLP profiles and tables with restriction fragment lengths for several diagnostic enzymes are provided for identification. Some problems of taxonomy and phylogeny of nematodes of the family Trichodoridae are also discussed. © 2012 The Authors. Plant Pathology © 2012 BSPP.

Lonsdale O.,Plant Pest Diagnostics Center | Lonsdale O.,Agriculture and Agri Food Canada
Zootaxa | Year: 2011

The Californian species of Liriomyza Mik are revised, including descriptions, illustrations, photographs and a key to species. Sixty-three species are now known to occur in the state, 12 of which are described here as new: L. bispinula, L. conclavis, L. cunicularia, L. merga, L. miserabilis, L. nebulosa, L. parabella, L. phyllodes, L. projecta, L. salpingion, L. tricornis and L. trixivora. Liriomyza virginica Spencer is included as a junior synonym of L. helianthi Spencer, and L. similis Spencer is included as a synonym of L. artemisiae Spencer. Two species are newly recorded in the United States: L. equiseti Meijere, previously known from Canada and Europe, and L. montana Sehgal, previously known from Canada. A number of specimens of L. brassicae (Riley) have been identified as potential new host "races" or species. Morphological characters are provided to diagnose the sister species L. huidobrensis (Blanchard) and L. langei Frick, previously recognizable only on the basis of molecular data. Numerous new state, county and host records are also presented, and hosts are compared for five of the most common North American agricultural pests: L. brassicae, L. huidobrensis, L. langei, L. sativae Blanchard and L. trifolii (Burgess). California contains the highest diversity of Liriomyza known to occur in North America, containing approximately 70% of all described species known from the lower 48 states. Copyright © 2011. Magnolia Press.

The levis and ferruginea groups of Orthemis are redefined. Six new species of Orthemis are described: O. aciculata sp. nov. (♂ holotype: Surinam, Para Dist., road near forest, Zanderij I (5°32'N, 55°10'W), 17 January 1957, leg. J. Belle [RMNH]), O. celata sp. nov. (♂ holotype: Brazil, Pará State, Rio Gurupí, Canindé (0°30′57″S, 51°14′00″W), 27-28 February 1966, leg. B. Malkin [RMNH]), O. faaseni sp. nov. (♂ holotype: Brazil, Rondônia State, Porto Velho (8°46′S, 63°54′W), 28 February 1922, leg. J.H.Williamson &J.W. Strohm [UMMZ]), O. garrisoni sp. nov. (♂holotype: Panama, Panamá Prov., 7 km NWof Gamboa, Pipeline Road, trail near palm forest, 30 July 1979, leg. RWG&J.A. Garrison [FSCA]), O. paulsoni sp. nov. (♂holotype: Ecuador, Sucumbíos Prov., forest trail ca. 3 km Wof Shushufindi (0°09′59″N, 76°41′30″W), 14 October 2009, leg. K.J. Tennessen [FSCA]), and O. teres sp. nov. (♂ holotype: Bolivia, Cochabamba Dept., Chapare, Cristal Mayo (17°00′57″N, 65°38′09″W), October/November 1994, leg. R. Andreas [FSCA]). Orthemis plaumanni Buchholz is found to be a junior synonym of O. ambinigra Calvert. A lectotype is designated for O. concolor Ris. All species of the levis group and the new species of the ferruginea group described here are diagnosed, illustrated, mapped, and keyed. © 2012 Worldwide Dragonfly Association.

Borkent C.J.,Plant Pest Diagnostics Center | Wheeler T.A.,McGill University
Systematic Entomology | Year: 2013

The monophyly and phylogenetic relationships within the species rich Sciophilini (Diptera: Mycetophilidae) were analysed, based on 96 adult morphological characters. The cladistic analysis included 80 Sciophilini exemplar species (representing all but 1 of the 36 genera placed previously in the Sciophilini) and 11 outgroup taxa of other mycetophilid tribes. The monophyly of Sciophilini was supported in the parsimony analysis by four synapomorphies. The tribe now contains 34 genera: Acnemia Winnertz, Acomoptera Vockeroth, Adicroneura Vockeroth, Afrocnemia Matile, Allocotocera Mik, Anaclileia Meunier, Aneura Marshall, Austrosciophila Tonnoir, Azana Walker, Baeopterogyna Vockeroth, Cluzobra Edwards, Drepanocercus Vockeroth, Duretophragma Borkent gen.n., Eudicrana Loew, Leptomorphus Curtis, Loicia Vockeroth, Megalopelma Enderlein, Monoclona Mik, Morganiella Tonnoir & Edwards, Neoallocotocera Tonnoir, Neoaphelomera Miller, Neotrizygia Tonnoir & Edwards, Neuratelia Rondani, Paramorganiella Tonnoir, Paratinia Mik, Paratrizygia Tonnoir, Parvicellula Marshall, Phthinia Winnertz, Polylepta Winnertz, Sciophila Meigen, Stenophragma Skuse, Tasmanina Tonnoir, Taxicnemis Tonnoir & Edwards, and Trizygia Skuse. Four genera placed previously in Sciophilini (Coelophthinia Edwards, Impleta Plassmann, Speolepta Edwards and Syntemna Winnertz) are transferred to the Gnoristini. Neoneurotelia Shinji and Neoparatinia Shinji are considered nomina dubia. Diagnoses are given for all genera in the tribe. Duretophragmagen.n. is described for the following species (all of which are comb.n.): Duretophragma andina (Duret), Duretophragma argentina (Duret), Duretophragma glabanum (Johannsen), Duretophragma fusca (Edwards), Duretophragma humeralis (Edwards), Duretophragma intermedia (Edwards), Duretophragma longifurcata (Freeman) (type species), Duretophragma morigenea (Edwards), Duretophragma naumanni (Duret), Duretophragma nigricauda (Edwards), Duretophragma obscura (Duret), Duretophragma ochracea (Freeman), Duretophragma pleuralis (Edwards) and Duretophragma similis (Johannsen). Other new generic combinations include: Trizygia albidens (Oliveira & Amorim) comb.n., Trizygia alvesi (Oliveira & Amorim) comb.n., Trizygia balbi (Oliveira & Amorim) comb.n., Trizygia camargoi (Oliveira & Amorim) comb.n. and Afrocnemia stellamicans (Chandler) comb.n. © 2013 The Royal Entomological Society.

Alvarez-Ortega S.,University of Jaén | Alvarez-Ortega S.,Plant Pest Diagnostics Center | Subbotin S.A.,Plant Pest Diagnostics Center | Pena-Santiago R.,University of Jaén
Nematology | Year: 2013

This contribution presents a study of Iberian and Californian populations of Aporcelaimellus simplex, including morphological, morphometric and molecular data. The species is characterised and distinguished by its lip region offset by an expansion, pars refringens vaginae absent, and conical tail with a small but distinct hyaline terminal portion. No significant difference exists between American and European populations. Molecular data and the derived evolutionary tree show a topology in which this species forms a well-supported group with members of Discolaiminae, far from other representatives of Aporcelaimellus. Putting special emphasis in the absence of pars refringens vaginae, A. simplex is transferred to the genus Aporcella. The taxonomy of this genus is revised in depth, with the proposal of an emended diagnosis, the provision of a list of 13 valid species (mostly new combinations from Aporcelaimellus) as well as a key to their identification and a compendium of their main morphometrics. Aporcella debruinae sp. n. is proposed for Aporcelaimellus papillatus apud de Bruin & Heyns, 1992. Copyright © Koninklijke Brill NV, Leiden, 2013.

Winterton S.L.,Plant Pest Diagnostics Center
ZooKeys | Year: 2011

A new species of Prepseudatrichia Kelsey, 1969 (Prepseudatrichia tiger sp. n.) is described from Thailand, the first record of the genus from the Oriental region. A key to world species of Prepseudatrichia is given. © Shaun L. Winterton.

Borkent C.J.,Plant Pest Diagnostics Center | Wheeler T.A.,McGill University
Zootaxa | Year: 2012

The world fauna of the genus Leptomorphus Curtis, 1831 is revised and a phylogeny of species relationships, based on morphological characters, is presented. An updated genus diagnosis and description are given. Species descriptions, diagnoses, illustrations of general habitus, wings, male genitalia and distributions are provided for 37 valid species, along with a key to adults. Twelve new species are described; L. amorimi Borkent, n. sp., L. brandiae Borkent, n. sp., L. crassipilus Borkent, n. sp., L. eberhardi Borkent, n. sp., and L. waodani Borkent, n. sp., from the Neotropical realm, L. furcatus Borkent, n. sp.,and L. perplexus Borkent, n. sp., from the Nearctic realm, L. mandelai Borkent, n. sp., and L. stigmatus Borkent, n. sp., from the Afrotropical realm, and L. tabatius Borkent, n. sp., L. tagbanua Borkent, n. sp., and L. titiwangsensis Borkent, n. sp., from the Oriental realm. Type specimens were studied for all but three species (L. ornatus, L. subforcipatus and L. talyshensis). Leptomorphus elegans Matile and L. lepidus Matile are considered junior synonyms of L. gracilis Matile, n. syns., and L. ypsilon Johannsen is a junior synonym of L. hyalinus Coquillett, n. syn. Lectotypes are designated for L. magnificus (Johannsen), L. neivai Edwards, and L. walkeri Curtis and a neotype is selected for L. bifasciatus (Say). This study brings the total number of extant Leptomorphus species to 45, including eight, unique (based on figures and descriptions), recently described Oriental and northwestern Australasian species (Papp & Ševcík 2011), for which material was unavailable for this study. The phylogenetic analysis in this study supports the monophyly of Leptomorphus. The western Nearctic species, L. perplexus, is the sister group to the remaining species, which fell into four major monophyletic species groups (L. ornatus Brunetti group, L. grjebinei Matile group, L. walkeri group, L. furcatus group). The monophyletic relationships within each group are also discussed. The clades found in this study do not support the arrangement of species in to the Leptomorphus subgenera recognized by previous classifications. © 2012 Magnolia Press.

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