Kumari S.,Czech Republic Crop Research Institute |
Subbotin S.A.,Plant Pest Diagnostics Center |
Subbotin S.A.,RAS Severtsov Institute of Ecology
Plant Pathology | Year: 2012
Plant parasitic nematodes of the family Trichodoridae cause substantial yield losses in many agricultural crops. Rapid and accurate identification of trichodorids to the species level is critical for selection of appropriate measures for control. This study analysed 99 sequences of the D2-D3 expansion segments of the 28S rRNA gene and 131 sequences of the 18S rRNA gene from the stubby nematodes belonging to the genera Nanidorus, Paratrichodorus and Trichodorus. Species delimiting was based on the integration of morphological identification, which is not provided in the present article, and molecular-based phylogenetic inference and sequence analysis. Twenty-two valid species and several species complexes were identified among nematodes included in the analysis. PCR-RFLPs of the partial 18S rDNA and the D2-D3 expansion segments of the 28S rDNA were tested and proposed for identification of these nematodes. Gel PCR-RFLP profiles and tables with restriction fragment lengths for several diagnostic enzymes are provided for identification. Some problems of taxonomy and phylogeny of nematodes of the family Trichodoridae are also discussed. © 2012 The Authors. Plant Pathology © 2012 BSPP.
Lonsdale O.,Plant Pest Diagnostics Center |
Lonsdale O.,Agriculture and Agri Food Canada
Zootaxa | Year: 2011
The Californian species of Liriomyza Mik are revised, including descriptions, illustrations, photographs and a key to species. Sixty-three species are now known to occur in the state, 12 of which are described here as new: L. bispinula, L. conclavis, L. cunicularia, L. merga, L. miserabilis, L. nebulosa, L. parabella, L. phyllodes, L. projecta, L. salpingion, L. tricornis and L. trixivora. Liriomyza virginica Spencer is included as a junior synonym of L. helianthi Spencer, and L. similis Spencer is included as a synonym of L. artemisiae Spencer. Two species are newly recorded in the United States: L. equiseti Meijere, previously known from Canada and Europe, and L. montana Sehgal, previously known from Canada. A number of specimens of L. brassicae (Riley) have been identified as potential new host "races" or species. Morphological characters are provided to diagnose the sister species L. huidobrensis (Blanchard) and L. langei Frick, previously recognizable only on the basis of molecular data. Numerous new state, county and host records are also presented, and hosts are compared for five of the most common North American agricultural pests: L. brassicae, L. huidobrensis, L. langei, L. sativae Blanchard and L. trifolii (Burgess). California contains the highest diversity of Liriomyza known to occur in North America, containing approximately 70% of all described species known from the lower 48 states. Copyright © 2011. Magnolia Press.
von Ellenrieder N.,Plant Pest Diagnostics Center
International Journal of Odonatology | Year: 2012
The levis and ferruginea groups of Orthemis are redefined. Six new species of Orthemis are described: O. aciculata sp. nov. (♂ holotype: Surinam, Para Dist., road near forest, Zanderij I (5°32'N, 55°10'W), 17 January 1957, leg. J. Belle [RMNH]), O. celata sp. nov. (♂ holotype: Brazil, Pará State, Rio Gurupí, Canindé (0°30′57″S, 51°14′00″W), 27-28 February 1966, leg. B. Malkin [RMNH]), O. faaseni sp. nov. (♂ holotype: Brazil, Rondônia State, Porto Velho (8°46′S, 63°54′W), 28 February 1922, leg. J.H.Williamson &J.W. Strohm [UMMZ]), O. garrisoni sp. nov. (♂holotype: Panama, Panamá Prov., 7 km NWof Gamboa, Pipeline Road, trail near palm forest, 30 July 1979, leg. RWG&J.A. Garrison [FSCA]), O. paulsoni sp. nov. (♂holotype: Ecuador, Sucumbíos Prov., forest trail ca. 3 km Wof Shushufindi (0°09′59″N, 76°41′30″W), 14 October 2009, leg. K.J. Tennessen [FSCA]), and O. teres sp. nov. (♂ holotype: Bolivia, Cochabamba Dept., Chapare, Cristal Mayo (17°00′57″N, 65°38′09″W), October/November 1994, leg. R. Andreas [FSCA]). Orthemis plaumanni Buchholz is found to be a junior synonym of O. ambinigra Calvert. A lectotype is designated for O. concolor Ris. All species of the levis group and the new species of the ferruginea group described here are diagnosed, illustrated, mapped, and keyed. © 2012 Worldwide Dragonfly Association.
Kumari S.,Czech Republic Crop Research Institute |
Subbotin S.A.,Plant Pest Diagnostics Center
European Journal of Plant Pathology | Year: 2012
Longidorus helveticus was found at two out of 285 sampling sites for the first time in the Czech Republic. Females, males and juvenile stages were analyzed morphologically and morphometrically. The morphological identification of samples was verified by polymerase chain reaction using a species specific primer. Four markers of ribosomal DNA (18S, ITS1, ITS2, D2-D3 expansion segments of 28S rRNA) and two markers of mitochondrial DNA (cox1 and nad4) were sequenced and analyzed and compared with published gene sequences of other populations of L. helveticus. The partial mitochondrial cytochrome c-oxidase subunit 1 gene and partial nicotinamide dehydrogenase subunits 4 gene showed relatively high genetic variation within the species compared with ribosomal DNA markers. © 2012 KNPV.
Winterton S.L.,Plant Pest Diagnostics Center
ZooKeys | Year: 2011
A new species of Prepseudatrichia Kelsey, 1969 (Prepseudatrichia tiger sp. n.) is described from Thailand, the first record of the genus from the Oriental region. A key to world species of Prepseudatrichia is given. © Shaun L. Winterton.