Plant Pathology Research Institute

Faisalābād, Pakistan

Plant Pathology Research Institute

Faisalābād, Pakistan
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Azmat M.A.,Vegetable Research Institute | Nawab N.N.,Vegetable Research Institute | Niaz S.,Vegetable Research Institute | Rashid A.,Vegetable Research Institute | And 3 more authors.
International Journal of Vegetable Science | Year: 2010

Powdery mildew caused by Erysiphe polygoni DC causes severe reductions in the green pod yield of pea (Pisum sativum L). The objective of this study was to reveal the genetics of powdery mildew resistance in six Resistant × Susceptible crosses and their generations (F1, F2, BCs, and BCr) against three isolates of E. polygoni. Cautious selection of the parental material and evaluation of 873 individual F2 plants from six crosses gave a perfect 3 susceptible:1 resistant segregation and 1 resistant:1 susceptible in BCr. These results led to the conclusion that resistance was conferred by the single recessive gene (er). © Taylor and Francis Group, LLC.


Akhtar K.P.,Nuclear Institute for Agriculture and Biology NIAB | Saleem M.Y.,Nuclear Institute for Agriculture and Biology NIAB | Asghar M.,Nuclear Institute for Agriculture and Biology NIAB | Ali S.,Plant Pathology Research Institute | And 2 more authors.
Pakistan Journal of Botany | Year: 2012

The reaction of 82 tomato genotypes belonging to 8 Solanum and a Lycopersicon species against Phytophthora infestans causing late blight was determined using detached-leaf and whole-plant assays. None of the test genotypes was immune or highly resistant. Of the 82 commercial and wild genotypes only TMS-2 (male-sterile and characterized by indeterminate growth) belonging to Lycopersicon esculentum was resistant with severity index of 2.4 in the detached-leaf assay on 0-5 scale (where 5 was highly susceptible) and percent disease index (%DI) of 23.3% under the whole-plant assay. Among the remaining genotypes, 41 were susceptible and 40 were highly susceptible under the detached-leaf assay, while 18 were susceptible and 63 were highly susceptible under the whole-plant assay. However, there was a significant difference in %DI for genotypes under the whole-plant assay. The response of whole-plants to inoculation with P. infestans in the detached-leaf assay was similar in all cases. The overall screening results indicate that TMS-2 is a good source of resistance and it can be useful for the development of tomato hybrid cultivars resistant to late blight.


Yusuf M.J.,Fodder Research Institute | Nabi G.,Fodder Research Institute | Basit A.,Fodder Research Institute | Husnain S.K.,Plant Pathology Research Institute | Akhtar L.H.,Agricultural Research Station
Pakistan Journal of Agricultural Sciences | Year: 2012

Development of superior crop cultivars is ultimate goal of plant breeders. New variety of pearl millet "Sargodha Bajra-2011" was evolved at Fodder Research Institute Sargodha. After obtaining a uniform type of plants, the new variety was evaluated. On the basis of its high green fodder yield, the new variety was tested in Station Yield Trials (SYT) during 2003, 2004 and 2005. Simultaneously: it was also evaluated in different ecological zones throughout the Punjab during the years 2005. It was evaluated in Notational Uniform Fodder Yield Trials (NUFYT) during the years 2004, 2005 and 2006. In SYT, it produced fodder yield of 35.09-66.82 t ha-1 and out yielded the check i.e. 18-BY. In zonal testing, the data reflected that this variety out yielded all the lines/varieties and the standard check variety "18-BY" producing an average green fodder yield of 60.67 t ha-1 against 52.66 t ha-1 produced by the check variety 18- BY. On the basis of weighted average of three years, Sargodha Bajra-2011 yielded 43.7-51.3 t ha-1 green fodder yields against the check variety which yielded 39.9-47.8 t ha-1. Plant height of the new variety is 260 cm; number of leaves per main tiller are 14; leaf color is green; leaf area is 334.3 cm2 and stem thickness is 1.4 cm. It has an erect growth habit. It has better palatability and digestibility. The fodder contains 5.67% crude protein, 38.98% dry matter, 27.07 crude fibre and 3.3% ether extract. Its agronomic studies were done during 2005-09. Its DUS study was conducted in 2008-2009. This variety gives best yield when planted on 15th July at 30 cm apart rows supplied with 80-60 kg NP ha-1 and irrigation applied at 14 days interval.


Hussain M.,University of Agriculture at Faisalabad | Khan M.A.,University of Agriculture at Faisalabad | Hussain M.,Plant Pathology Research Institute | Javed N.,University of Agriculture at Faisalabad | Khaliq I.,University of Agriculture at Faisalabad
Journal of Animal and Plant Sciences | Year: 2016

Leaf, stripe and stem rust are considered one of the most important diseases of wheat in Pakistan. The use of rust resistant varieties is the most economical and environment friendly way to reduce losses due to rust diseases. For this purpose a study was planned to combine genes for enhancing rust resistance and high yield potential in wheat. The experiments for identification of durable rust resistance genes i.e. Sr2/Yr30, Lr34/Yr18, Lr46/Yr29 on the basis of phenotypic markers like pseudo black chaff (Pbc) and leaf tip necrosis (Ltn1 & Ltn2) were conducted in augmented design during the years 2010-11 and 2011-12 at Wheat Research Institute, Faisalabad and Summar Agricultural Research Station, Kaghan. Total 750 head rows were selected from F6 generation and planted at SARS, Kaghan. The outstanding 345 lines were selected and planted for further evaluation and on the basis of rust reactions, phenotypic uniformity and high yield under the field conditions, 220 lines were selected for further testing. The results indicate that among 220 entries, 67 entries showed Lr34/Yr18, 23 entries showed Lr46/Yr29 and 62 entries showed Sr2/Yr30 genes combination. While in yield testing, among test entries, 99 entries were found to be high yielding ranging from 3973-4786 kg/ha. From the study, it is concluded that fourteen test entries i.e. V-11211,V-11212, V-11218, V-11227, V-11262, V-11288, V-11296, V-11304, V-11308, V-11319, V-11338, V-11353, V-11365 and V-11396 are the most prominent lines because these lines showed the combination of all three slow rusting genes as well as high yield potential. These selected genotypes will be very useful for the wheat breeders and pathologist of the country in planning of future hybridization program. © 2016, Pakistan Agricultural Scientists Forum. All rights reserved.


Rehman A.,University of Agriculture at Faisalabad | Imran M.,University of Agriculture at Faisalabad | Mehboob S.,Plant Pathology Research Institute | Khan N.A.,University of Agriculture at Faisalabad | And 2 more authors.
International Journal of Agriculture and Biology | Year: 2015

Collar rot induced by Macrophomina phaseolina, recently appeared as one of the most significant constraints to the production of Cockscomb (Celosia argentea L.), an ornamental plant. Symptoms include, wilting of the plant, chlorosis of lower leaves, shrinking of stem at collar region, which becomes black at later stage and affected plant become shredded, consequently a clear mycelial growth of the fungus can be seen if the stem of the affected plant is split open. In this study, the causal fungus was isolated from the diseased tissues (collar and stem regions, externally and internally). Then the pathogenicity test of isolated pathogens was confirmed through Koch's postulates. Considering the management trials, five fungicides (Carbendazim, Thiophenate-M, Ridomil Gold, Antracol and Score) and five different plant extracts (Azadirachta indicia L., Melia azedarach L., Eucalyptus cameldulensis L., Syzygium cumini L. and Moringa oleifera L.) were evaluated against the collar rot disease pathogen (Macrophomina phaseolina) by poison food technique at different concentrations viz., 100, 150, 200 and 250 μg/mL for fungicides and 10, 15 and 20% for plant extracts. Out of five fungicides used, Topsin M and Antracol were found to be most effective in reducing the mycelial growth of M. phaseolina by (83%) and (76%) followed by Ridomil Gold, Score and Carbendazim (46, 39 and 29% reduction). In case of plant extracts, A. indicia and E. cameldulensis extracts were more effective by reducing the mycelial growth of M. phaseolina by 77 and 74%, respectively, followed by M. oleifera, M. azedarach, and S. cumini (61, 45 and 18% reduction). © 2015 Friends Science Publishers.


Anwar-Ul-Haq M.,University of Agriculture at Faisalabad | Anwar S.A.,University of Agriculture at Faisalabad | Shahid M.,Plant Pathology Research Institute | Javed N.,University of Agriculture at Faisalabad | And 2 more authors.
Pakistan Journal of Zoology | Year: 2011

This study was planned to assess the efficacy of plant growth promoting rhizobacteria (PGPR) against Meloidogyne incognita infection on roots of tomato (Lycopersicon esculentum Mill.) in the green house at 30 + 4 o C. Fifteen-days-old seedlings of tomato cultivar "Money Maker" were planted singly in 15-cm-diam pots filled with sterilized sandy soil. Two days after transplanting, 20-ml of 5% sugar solution containing 107 CFU/ml each of Bacillus spp., Azotobacter spp., Pseudomonas putida and P. fluorescens, were pipetted into three 3-cm deep holes surrounding the root zone of each plant. Five days after the application of PGPR, freshly hatched 2000 J2 were applied at root zone. The experiment consisted of seven treatments; each with seven replicates and arranged in CRD. Pots with nematodes, without nematodes, and PGPR were kept as control for comparison. Sixty days after inoculations, data of plant growth parameters such as plant height, fresh and dry root and shoot weight and nematode reproduction in term of egg masses per root system, galls per root system, J2/one gm of root and females per root system were recorded. The plants treated with P. fluorescens significantly (P = 0.05) suppressed females per root system (40.52%), J2/one gm of root (39.80%), galls per root system (41.50%) and egg masses per root system (43.23%) resulting in improved growth over control plants. The treatments having P. putida, Bacillus spp. and combination of PGPR showed intermediary effects on both nematode reproduction and plant growth. Azobacter spp. was least effective in suppressing only 28.10%, 15.87%, 29.38%, 29.29% females per root system, J2 per gm of root, galls per root system and egg masses per root system, respectively. © 2011 Zoological Society of Pakistan.


Hussain M.,Wheat Research Institute | Khan M.A.,University of Agriculture at Faisalabad | Hussain M.,Plant Pathology Research Institute | Javed N.,University of Agriculture at Faisalabad | Khaliq I.,University of Agriculture at Faisalabad
Archives of Phytopathology and Plant Protection | Year: 2015

The idea of this study was to be vigilant with rust virulence pattern changes in the region and to organise wheat breeding and variety release programmes in the light of findings of rust virulence patterns. The survey was carried out during March–April (2010 and 2011) on the rust trap nurseries planted at different Agra-ecological condition in Pakistan. The rust trap nursery consisted of 40 Lr differentials and 24 Yr differentials and data were recorded following modified Cobb’s scale. The studies revealed that there were no virulence for Lr9, Lr19, Lr23+, Lr25, Lr28, Lr27+31 Lr32, Lr34, Lr36 and Lr37 and Yr3, Yr5, YR8, Yr10, Yr15, Yr18, and Yrsp observed during this period. Among the varieties Iqbal-2000, Uqab-2000, Lasani-2008, Faisalabad-2008, AARI-2011, Millat-2011 and Pb-2011 exhibited resistance path for LR and YR. Moderate YR was observed on Shafaq-2006 & Chenab-2000 ranging from 20–30 M RMS. In these studies the susceptible check variety Morocco was almost 80–100S for LR and YR. © 2014, © 2014 Taylor & Francis.


Hussain M.,University of Agriculture at Faisalabad | Khan M.A.,University of Agriculture at Faisalabad | Hussain M.,Plant Pathology Research Institute | Javed N.,University of Agriculture at Faisalabad | Khaliq I.,University of Agriculture at Faisalabad
International Journal of Agriculture and Biology | Year: 2015

In order to combine genes for enhancing rust resistance and high yield potential in wheat, parent lines were selected for hybridization on the basis of slow rusting history and phenotypic characters for durable resistance. The hybridized germplasm was advanced in filial generations from F1 to F5. Total 750 head rows were planted in F6 from selected heads among F5 generation. From 750 single head rows planted in Kaghan, 345 lines were selected on the basis of agronomic traits and rust resistance; two hundred and twenty lines were selected for high yield and rust resistance performance and evaluated for the presence of durable rust resistant genes with molecular markers. It was confirmed that the lines showing durable rust resistance possessed Lr34/Yr18, Lr46/Yr29 and Sr2/Yr30 genes in combination or individuals from these lines. The results indicated that the most prominent lines i.e., V-11211, V-11212, V-11218, V-11227, V-11262, V-11288, V-11296, V-11304, V-11308, V-11319, V-11338, V-11353, V-11365 and V-11396 showed the combination of three designated slow rusting genes. These lines were high yielding with better resistance than all existing approved wheat varieties of the country. None of these lines had complete resistance, but were of slow rusting type and were suitable for commercial cultivation. These results will be useful for wheat breeders and pathologists of the country in planning of future hybridization program. © 2015 Friends Science Publishers.


Naqvi S.A.,University of Agriculture at Faisalabad | Khan M.M.,University of Agriculture at Faisalabad | Jaskani M.J.,University of Agriculture at Faisalabad | Mahmood K.,Plant Pathology Research Institute | And 3 more authors.
Pakistan Journal of Agricultural Sciences | Year: 2015

Studies were conducted to assess the role of mucilage in seed des iccation, germination, and its interaction with fungus in Rough lemon, Sachtion citrumelo and Yuma citrange rootstock seeds of citrus fruits. Water loss percentage was minimum (with or without mucilage) in Rough lemon seeds (20.28%) and (31.25%) at 25°C an d 35°C respectively, with high germination percentage (3.33%) while maximum water loss (29.50%) was observed in Sachtion cit rumelo at 25°C having low germination percentage (1.33%). However, Yuma citrange showed maximum water loss (34.39%) at 35°C with germination percentage (1.67%). The pathogenic fungus which infected the seeds was isolated, purified and identified as Aspergillus niger. Fungus growth on seeds of Rough lemon, Sachtion citrumelo and Yuma citrange was 14.08 mm, 10.65 mm and 9.36 mm, respectively on 6th day of inoculation. It is obvious from the results that mucilage played significant role in desiccation, germination as well as fungal habitation. © 2015, University of Agriculture. All right reserved.


Akhtar K.P.,Nuclear Institute for Agriculture and Biology | Sarwar N.,Nuclear Institute for Agriculture and Biology | Saleem K.,Nuclear Institute for Agriculture and Biology | Ali S.,Plant Pathology Research Institute
Australasian Plant Disease Notes | Year: 2013

Trianthema portulacastrum (horse purslane) is among the most noxious summer annual weeds in Pakistan. During June 2010-2012, a severe outbreak of leaf and stem blight was observed for the first time on T. portulacastrum in Pakistan. Symptoms on leaves and stems were examined as round to oval straw colored spots with maroon margins. As the disease progressed, affected leaves became chlorotic and dried up causing severe defoliation and withering of stems. The pathogen was identified as Gibbago trianthemae on the basis of morphology and cultural characteristics. A pathogenicity test was performed and Koch's postulates were fulfilled by re-isolation of the fungus G. trianthemae from diseased tissues of T. portulacastrum. This is the first report of G. trianthemae causing blight on T. portulacastrum in Pakistan. © 2013 Australasian Plant Pathology Society Inc.

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