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We present a multiplex polymerase chain reaction method to differentiate between three Japanese internal apple-feeding pests: the oriental fruit moth, Grapholita molesta (Busck), Grapholita dimorpha Komai, and the peach fruit moth, Carposina sasakii Matsumura. A 1,342-bp fragment of mitochondrial cytochrome oxidase subunit I (COI) was sequenced in each species. The three species showed consistent and diagnostic differences in the region of the COI gene, from which three species-specific forward primers were designed. The forward primers along with one universal reverse primer were used to selectively amplify DNA from specimens of diverse geographic origin for each corresponding target species. This method enabled easy, immediate, and accurate identification of internal feeding Lepidoptera in apples and other fruits. © 2011 The Japanese Society of Applied Entomology and Zoology. Source


Amrit R.,National Electronics and Computer Technology Center | Sripumkhai W.,Thai Microelectronic Center | Porntheeraphat S.,National Electronics and Computer Technology Center | Jeamsaksiri W.,Thai Microelectronic Center | And 2 more authors.
Proceedings of SPIE - The International Society for Optical Engineering | Year: 2013

Faster and reliable testing technique to count and identify nematode species resided in plant roots is therefore essential for export control and certification. This work proposes utilizing a multichannel microfluidic chip with an integrated flow-through microfilter to retain the nematodes in a trapping chamber. When trapped, it is rather simple and convenient to capture images of the nematodes and later identify their species by a trained technician. Multiple samples can be tested in parallel using the proposed microfluidic chip therefore increasing number of samples tested per day. © 2013 SPIE. Source


Webster C.G.,U.S. Department of Agriculture | Frantz G.,Glades Crop Care Inc. | Reitz S.R.,Oregon State University | Funderburk J.E.,University of North Florida | And 8 more authors.
Phytopathology | Year: 2015

Groundnut ringspot virus (GRSV) and Tomato chlorotic spot virus (TCSV) are two emerging tospoviruses in Florida. In a survey of the southeastern United States, GRSV and TCSV were frequently detected in solanaceous crops and weeds with tospovirus-like symptoms in south Florida, and occurred sympatrically with Tomato spotted wilt virus (TSWV) in tomato and pepper in south Florida. TSWV was the only tospovirus detected in other survey locations, with the exceptions of GRSV from tomato (Solanum lycopersicum) in South Carolina and New York, both of which are first reports. Impatiens (Impatiens walleriana) and lettuce (Lactuca sativa) were the only non-solanaceous GRSV and/or TCSV hosts identified in experimental host range studies. Little genetic diversity was observed in GRSV and TCSV sequences, likely due to the recent introductions of both viruses. All GRSV isolates characterized were reassortants with the TCSV M RNA. In laboratory transmission studies, Frankliniella schultzei was a more efficient vector of GRSV than F. occidentalis. TCSV was acquired more efficiently than GRSV by F. occidentalis but upon acquisition, transmission frequencies were similar. Further spread of GRSV and TCSV in the United States is possible and detection of mixed infections highlights the opportunity for additional reassortment of tospovirus genomic RNAs. Source


Bhasabutra T.,Plant Pathology Research Group | Seemadua S.,Plant Pathology Research Group | Shivas R.G.,Plant Pathology Herbarium
Australasian Plant Disease Notes | Year: 2012

The rust Prospodium appendiculatum was collected on Tecoma stans, commonly called yellow bells or yellow trumpet bush, from northeastern Thailand. The identification was based on the morphology of uredinial and telial stages. This is the first report of this rust fungus in Thailand where T. stans is often cultivated as an ornamental. © 2012 Australasian Plant Pathology Society Inc. Source


Seemadua S.,Plant Pathology Research Group | Bhasabutra T.,Plant Pathology Research Group | Beasley D.R.,Plant Pathology Herbarium | Tan Y.P.,Plant Pathology Herbarium | Shivas R.G.,Plant Pathology Herbarium
Australasian Plant Disease Notes | Year: 2012

Leaves of Afzelia xylocarpa infected with rust were collected from the National Corn and Sorghum Research Centre, Rai Suwan, Nakhon Ratchasima, north-eastern Thailand in January 2011. The rust was identified as Phakopsora pachyrhizi based on morphological and DNA sequence data. Afzelia xylocarpa represents a new host genus and species for this rust fungus, which is commonly known as Asian soybean rust. © 2012 Australasian Plant Pathology Society Inc. Source

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