Plant Molecular Biology Research Unit

Palayamkottai, India

Plant Molecular Biology Research Unit

Palayamkottai, India
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Haniffa M.A.,Center for Aquaculture Research and Extension | Sheela P.J.,Center for Aquaculture Research and Extension | Milton M.J.,Center for Aquaculture Research and Extension | De Britto J.,Plant Molecular Biology Research Unit
International Journal of Pharmacy and Pharmaceutical Sciences | Year: 2013

Murrels are often affected by the dreadful disease Epizootic Ulcerative Syndrome (EUS) and the primary causative agent of EUS is the fungus Aphanomyces invadans and the opportunistic bacterial pathogen is Aeromonas hydrophila. The antibacterial activity and antifungal activity of W. tinctoria were studied against selected pathogens and invivo antibiotic effect in EUS affected striped murrel Channa striatus was also examined. The leaves of a medicinal herb namely Wrightia tinctoria (Roxb.) R. Br. was evaluated against five fish pathogens. Phytochemical screening of the leaves revealed the presence of steroids, reducing sugars, alkaloids, phenolic compounds, flavonoids, saponins and tannins. The TLC chromatogram of the extracts revealed several coloured bands. Antibacterial activities of the extracts were determined by the agar disc diffusion method. Chlortetracycline (5mcg/disc) was used as positive control for comparison of the inhibition zones. The extract showed efficient antibacterial activity against Aeromonas hydrophila with maximum zone of inhibition. The Minimum Inhibitory Concentration of the extract against the pathogens was determined.Growth of Aphanomyces invadans was inhibited by 85-90%. The herbal paste applied topically on the lesions showed a positive effect by controlling and curing the lesions. Thus, the active extract of W. tinctoria could be taken to the next step of bioassay guided purification to characterize the novel antimicrobial agents.


John de Britto A.,Plant Molecular Biology Research Unit | Sebastian S.R.,Plant Molecular Biology Research Unit
International Journal of Pharmacy and Pharmaceutical Sciences | Year: 2013

Research on nanoparticles is currently an area of intense scientific research, due to a wide variety of potential applications in biomedical, optical, and electronic fields. Synthesis of nanoparticles can be carried out by using various chemical and physical methods. But use of such methods is harmful in one or the other way as the chemicals often used are toxic, flammable, not easily disposable due to environmental issues, having low production rate, etc. As a result, a great deal of effort has been put into the search for methods utilizing biological systems, such as the micro-organisms and plants, for the synthesis of metal nanoparticles. Such biologically synthesized silver nanoparticles from Apocynaceae members were evaluated against the human pathogens. The plant based silver nano particles synthesized from Rauvolfia tetraphylla was found to be most active against Shigella dysenteriae with the highest zone of inhibition.


Herin Sheeba Gracelin D.,Plant Molecular Biology Research Unit | John de Britto A.,Plant Molecular Biology Research Unit | Benjamin Jeya Rathna Kumar P.,Plant Molecular Biology Research Unit
International Journal of Pharmacy and Pharmaceutical Sciences | Year: 2013

Objectives: The objective of the present study was to find out the presence of phytochemicals in the methanol extracts of five ferns in Pteridaceae family such as Pteris argyreae T. Moore, Pteris confusa T.G. Walker, Pteris vittata L., Pteris biaurita L., and Pteris multiaurita Ag., by both qualitative and quantitative screening methods. Methods: In qualitative analysis, the phytochemical compounds such as steroids, reducing sugars, triterpenoids, sugars, alkaloids, phenolic compounds, flavonoids, saponins, tannins, anthroquinones and amino acids were screened in five ferns extracts by using standard methods. Results: The methanol extract of the fern Pteris biaurita showed positive results for 10 phytochemical tests. Pteris vittata extract exhibited positive results for 9 tests. In methanol extracts of the Pteris argyreae, Pteris confusa showed positive results for 7 tests and in the extract of Pteris multiaurita 5 phytochemical tests were positive. In quantitative analysis the important secondary metabolites such as alkaloids, phenolic compounds, flavonoids, saponins and tannins were tested in all the ferns extracts. The methanol extract of Pteris biaurita showed highest amount of phytochemicals when compared with other solvent extracts. Conclusions: More active compounds will be isolated from the selected ferns and they may be used for medicinal purposes in future.


John de Britto A.,Plant Molecular Biology Research Unit | Sebastian S.R.,Plant Molecular Biology Research Unit | Mary Sujin R.,Plant Molecular Biology Research Unit
International Journal of Pharmacy and Pharmaceutical Sciences | Year: 2011

The antibacterial activity of the methanol extracts of seven selected plant species belonging to the family Apocynaceae, collected from different localities of Tirunelveli district of Tamilnadu were screened against ten infectious pathogens. Antibacterial activities of the extracts were determined by the agar disc diffusion method. MIC of the plant extracts that showed some efficacy against pathogens at the minimum concentration was noted. Ciprofloxacin (5 mcg/disc) was used as positive control for comparison of the inhibition zones. The methanolic extracts of A. cathartica and P. alba showed active inhibition against most of the tested human pathogens.


Herin Sheeba Gracelin D.,Plant Molecular Biology Research Unit | John de Britto A.,Plant Molecular Biology Research Unit | Benjamin Jeya Rathna Kumar P.,Plant Molecular Biology Research Unit
International Journal of Pharmacy and Pharmaceutical Sciences | Year: 2012

Xanthomonas campestris pv. centellae (X.c.pv.c) is a harmful phytopathogen which causes leaf spot disease on Centella plants (Centella asiatica). To control this harmful pathogen in biocontrol method, methanol and aqueous extracts of twenty south Indian flowers were evaluated for their antibacterial activity against the harmful pathogen which was isolated from the infected Centella plant in disc diffusion method in five different concentrations (5μg/ml, 10μg/ml, 20μg/ml, 40μg/ml and 80μg/ml). Among these, the methanol and aqueous extracts of Punica granatum, Carica papaya, Thevetia peruviana, Catharanthus roseus and Calotropis gigantea showed significant inhibitory effect (p<0.05). Based on the MIC values, C. papaya (8μg/ml) and C. roseus (16μg/ml) were selected for biocontrol agents against X.c.pv.c.


Britto A.J.A.,Plant Molecular Biology Research Unit | Mary Sujin R.,Plant Molecular Biology Research Unit
International Journal of Pharmacy and Pharmaceutical Sciences | Year: 2012

High Performance Liquid chromatography was done for quantification of vitexin and for examining the fingerprint of Vitex negundo methanol leaf extract. The analysis was carried out using Lichrosorb C-18 column at 25°C, with methanol: water as mobile phase. The flow rate was 1.0 ml/min and the detection was at 210 nm. The amount of vitexin present in V. negundo collected from five different locations of Western Ghats ranged from 22.91 to 83.69 μg/ml. Although the amount of the marker flavonoid (vitexin) was different, HPLC fingerprints of all samples were well conserved. The highest amount of vitexin was found in Karaiyar accession and was considered as superior genotype. The compiled data will be useful for taxonomy, to improve the quality control and authenticity testing of phytopharmaceuticals containing V. negundo extracts.


John De Britto A.,Plant Molecular Biology Research Unit | Herin Sheeba Gracelin D.,Plant Molecular Biology Research Unit | Benjamin Jeya Rathna Kumar P.,Plant Molecular Biology Research Unit
International Journal of Pharma and Bio Sciences | Year: 2013

The objective of the present study was to find out the presence of phytochemicals in the petroleum ether, benzene, chloroform, methanol and aqueous extracts of Marsilea minuta Linn (Marsileaceae), a common aquatic medicinal fern by both qualitative and quantitative screening methods. In qualitative analysis, the phytochemical compounds such as steroids,reducing sugars, triterpenoids, sugars, alkaloids, phenolic compounds, flavonoids, saponins, tannins, anthroquinones andamino acids were screened in five solvent extracts. The methanol extract of the fern showed positive results for 10 phytochemical tests. The benzene extract exhibited positive results for 9 tests. In chloroform and petroleum ether extracts of the plant showed positive results for 7 tests and in aqueous extract of the fern 5 phytochemical tests were positive.In quantitative analysis the important secondary metabolites such as alkaloids, phenolic compounds, flavonoids, saponinsand tannins were tested in all the extracts of the fern. The methanol extract showed highest amount of phytochemicals when compared with other solvent extracts.


de Britto A.J.,Plant Molecular Biology Research Unit | Herin Sheeba Gracelin D.,Plant Molecular Biology Research Unit | Sebastian S.R.,Plant Molecular Biology Research Unit
Journal of Biopesticides | Year: 2011

The methanol and aqueous extracts of leaves of six different medicinal plants, Acalypha indica, Aerva lanata, Phyllanthus amarus, Phyllanthus emblica, Cassia auriculata and Caesalpinia pulcherrima, were used for the investigation of antibacterial studies. In antibacterial screening performed by disc diffusion method against two types of bacteria namely Xanthomonas campestris (plant pathogen) and Aeromonas hydrophila (human pathogen), it was found that the methanol extracts of all the plant samples showed significant activity against the two tested bacteria. The methanol extracts of Acalypha indica, Aerva lanata and Phyllanthus amarus exhibited clear zone of inhibition against the tested micro organisms. Among these three samples, the MIC value of Aerva lanata, determined by serial dilution technique, was found to be 32μg/ml and 64μg/ml against Xanthomonas campestris and Aeromonas hydrophila respectively.


De Britto A.J.,Plant Molecular Biology Research Unit | Gracelin D.H.S.,Plant Molecular Biology Research Unit | Kumar P.B.J.R.,Plant Molecular Biology Research Unit
Asian Pacific Journal of Tropical Biomedicine | Year: 2012

Objective: To analyze the phytochemical constituents of five medicinal ferns Pteris biaurita (P. biaurita) L., Lygodium flexiuosam (L. flexiuosam) (L.) Sw., Hemionitis arifolia (H. arifolia) (Burm.f.) T. Moore, Actinopteris radiate (A. radiata) (J. Koenig ex Sw.) Link and Adiantum latifolium (A. latifolium) Lam. Methods: The shade dried and powdered fronds (50 g) were extracted successively with 250 mL of petroleum ether, benzene, chloroform, methanol and distilled water using a Soxhlet extractor for 8 h at a temperature of 50-60°C (not exceeding the boiling point of the solvent). Phytochemical analysis of the extracts was carried out according to the standard procedures. Results: Among the twenty five tested extracts, twenty extracts showed the presence of flavonoids. Phenolic compounds are present in methanol extracts of all the ferns. Five extracts showed the presence of triterpinoids and catechins. Ten extracts showed the occurrence of sugars and reducing sugars. Fifteen extracts showed the presence of alkaloids. Tannins and saponins are present in eight extracts. Steroids occurred in all the twenty five extracts. Only three extracts showed the presence of anthroquinones and amino acids. Conclusions: From this investigation, it can be concluded that the selected five medicinal ferns contain more bioactive principles. These potential ferns could be used as bio control agnets. © 2012 Asian Pacific Tropical Biomedical Magazine.


John de Britto A.,Plant Molecular Biology Research Unit | Herin Sheeba Gracelin D.,Plant Molecular Biology Research Unit
Asian Journal of Pharmaceutical and Clinical Research | Year: 2011

The phytochemical and antibacterial studies of the fruit extracts of Jasminum grandiflorum Linn. have been investigated. Antibacterial activity was tested using Xanthomonas campestris and Aeromonas hydrophila. The benzene, chloroform and aqueous extract were completely inactive. Methanol extract showed significant inhibitory effect against X.campestris and A.hydrophila. The phytochemical analysis revealed the presence of steroids, sugars, reducing sugars, alkaloids, phenolic compounds and tannins in the methanol extract of fruits. This study reports the methanol extracts of the fruit of the plant J. grandiflorum for biocontrol purposes against bacterial infection in plants and animals.

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