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John De Britto A.,Plant Molecular Biology Research Unit | Herin Sheeba Gracelin D.,Plant Molecular Biology Research Unit | Benjamin Jeya Rathna Kumar P.,Plant Molecular Biology Research Unit
International Journal of Pharma and Bio Sciences | Year: 2013

The objective of the present study was to find out the presence of phytochemicals in the petroleum ether, benzene, chloroform, methanol and aqueous extracts of Marsilea minuta Linn (Marsileaceae), a common aquatic medicinal fern by both qualitative and quantitative screening methods. In qualitative analysis, the phytochemical compounds such as steroids,reducing sugars, triterpenoids, sugars, alkaloids, phenolic compounds, flavonoids, saponins, tannins, anthroquinones andamino acids were screened in five solvent extracts. The methanol extract of the fern showed positive results for 10 phytochemical tests. The benzene extract exhibited positive results for 9 tests. In chloroform and petroleum ether extracts of the plant showed positive results for 7 tests and in aqueous extract of the fern 5 phytochemical tests were positive.In quantitative analysis the important secondary metabolites such as alkaloids, phenolic compounds, flavonoids, saponinsand tannins were tested in all the extracts of the fern. The methanol extract showed highest amount of phytochemicals when compared with other solvent extracts.


de Britto A.J.,Plant Molecular Biology Research Unit | Herin Sheeba Gracelin D.,Plant Molecular Biology Research Unit | Sebastian S.R.,Plant Molecular Biology Research Unit
Journal of Biopesticides | Year: 2011

The methanol and aqueous extracts of leaves of six different medicinal plants, Acalypha indica, Aerva lanata, Phyllanthus amarus, Phyllanthus emblica, Cassia auriculata and Caesalpinia pulcherrima, were used for the investigation of antibacterial studies. In antibacterial screening performed by disc diffusion method against two types of bacteria namely Xanthomonas campestris (plant pathogen) and Aeromonas hydrophila (human pathogen), it was found that the methanol extracts of all the plant samples showed significant activity against the two tested bacteria. The methanol extracts of Acalypha indica, Aerva lanata and Phyllanthus amarus exhibited clear zone of inhibition against the tested micro organisms. Among these three samples, the MIC value of Aerva lanata, determined by serial dilution technique, was found to be 32μg/ml and 64μg/ml against Xanthomonas campestris and Aeromonas hydrophila respectively.


De Britto A.J.,Plant Molecular Biology Research Unit | Gracelin D.H.S.,Plant Molecular Biology Research Unit | Kumar P.B.J.R.,Plant Molecular Biology Research Unit
Asian Pacific Journal of Tropical Biomedicine | Year: 2012

Objective: To analyze the phytochemical constituents of five medicinal ferns Pteris biaurita (P. biaurita) L., Lygodium flexiuosam (L. flexiuosam) (L.) Sw., Hemionitis arifolia (H. arifolia) (Burm.f.) T. Moore, Actinopteris radiate (A. radiata) (J. Koenig ex Sw.) Link and Adiantum latifolium (A. latifolium) Lam. Methods: The shade dried and powdered fronds (50 g) were extracted successively with 250 mL of petroleum ether, benzene, chloroform, methanol and distilled water using a Soxhlet extractor for 8 h at a temperature of 50-60°C (not exceeding the boiling point of the solvent). Phytochemical analysis of the extracts was carried out according to the standard procedures. Results: Among the twenty five tested extracts, twenty extracts showed the presence of flavonoids. Phenolic compounds are present in methanol extracts of all the ferns. Five extracts showed the presence of triterpinoids and catechins. Ten extracts showed the occurrence of sugars and reducing sugars. Fifteen extracts showed the presence of alkaloids. Tannins and saponins are present in eight extracts. Steroids occurred in all the twenty five extracts. Only three extracts showed the presence of anthroquinones and amino acids. Conclusions: From this investigation, it can be concluded that the selected five medicinal ferns contain more bioactive principles. These potential ferns could be used as bio control agnets. © 2012 Asian Pacific Tropical Biomedical Magazine.


John de Britto A.,Plant Molecular Biology Research Unit | Herin Sheeba Gracelin D.,Plant Molecular Biology Research Unit
Asian Journal of Pharmaceutical and Clinical Research | Year: 2011

The phytochemical and antibacterial studies of the fruit extracts of Jasminum grandiflorum Linn. have been investigated. Antibacterial activity was tested using Xanthomonas campestris and Aeromonas hydrophila. The benzene, chloroform and aqueous extract were completely inactive. Methanol extract showed significant inhibitory effect against X.campestris and A.hydrophila. The phytochemical analysis revealed the presence of steroids, sugars, reducing sugars, alkaloids, phenolic compounds and tannins in the methanol extract of fruits. This study reports the methanol extracts of the fruit of the plant J. grandiflorum for biocontrol purposes against bacterial infection in plants and animals.


John De Britto A.,Plant Molecular Biology Research Unit | Sebastian S.R.,Plant Molecular Biology Research Unit | Mary Sujin R.,Plant Molecular Biology Research Unit
Indian Journal of Natural Products and Resources | Year: 2012

Eight selected plant species belonging to the family Lamiaceae were collected from different localities of Tirunelveli district of Tamil Nadu, dried and extracted by crude methanol extraction method. The antimicrobial activity and Minimum Inhibitory Concentration (MIC) of the extracts were evaluated against ten human pathogens. Antimicrobial activities of the extracts were determined by the agar disc diffusion method. MIC was determined in the plant extracts that showed some efficacy against pathogens. Ciprofloxacin (5 mcg/disc) was used as positive control for comparison of the inhibition zones. The microbial activity of the methanolic extract of Coleus aromaticus Benth. was found efficient against all the tested pathogens. In Lamiaceae, C. aromaticus, Mentha arvensis Linn. and Leucas aspera Willd. had lower MIC values of 12.5 mg/ml with inhibition zones of 10, 10 and 8 mm, respectively.


Herin Sheeba Gracelin D.,Plant Molecular Biology Research Unit | John De Britto A.,Plant Molecular Biology Research Unit | Benjamin Jeya Rathna Kumar P.,Plant Molecular Biology Research Unit
Asian Journal of Pharmaceutical and Clinical Research | Year: 2012

A bacterial leaf spot disease has been the major problem in cultivation of C. asiatica in Tirunelveli districts (Tamilnadu) in India. Hence laboratory experiments were conducted to detect and identify the causative pathogen on leaves of Centella asiatica collected from naturally contaminated commercial plants. Two modified semi-selective media namely mTBM and mMD5A were used to detect the bacterium from the infected leaves. Identification of the strain was proved by using morphology, physiology and biochemical test. The results showed the presence of strain i.e. Xanthomonas campestris pv. centellae (X.c.pv.c) in all the assayed plants.


Herin Sheeba Gracelin D.,Plant Molecular Biology Research Unit | de Britto A.J.,Plant Molecular Biology Research Unit | Benjamin Jeya Rathna Kumar P.,Plant Molecular Biology Research Unit
Journal of Biopesticides | Year: 2012

The objective of the present study is to evaluate the antimicrobial potency of five solvents (petroleum ether, benzene, chloroform, methanol and water) extracts of five medicinal ferns Pteris biaurita L., Lygodium flexiuosam (L.) Sw., Hemionitis arifolia (Burm.F.) T. Moore, Actinopteris radiata (J. Koenig ex Sw.) Link and Adiantum latifolium Lam. against the gram negative plant pathogenic bacteria Xanthomonas campestris. Antimicrobial activity, Minimum Inhibitory Concentration (MIC) and Relative Percentage Inhibition (RPI) value of the extracts against X. campestris were screened according to the standard procedures. Among the twenty five tested extracts, methanol and benzene extracts of P. biaurita showed significant inhibition (p<0.05) against the tested bacteria. Based on the results of MIC (8μg/mL) and RPI (199.36%) values, it could be concluded that P. biaurita can be used as potential plant for the management of X. campestris which is known to cause leaf spot disease on many vegetables and cash crops particularly Centella asiatica. ©JBiopest.


De Britto A.J.,Plant Molecular Biology Research Unit | Gracelin D.H.S.,Plant Molecular Biology Research Unit | Benjamin P.,Plant Molecular Biology Research Unit | Kumar J.R.,Plant Molecular Biology Research Unit
Indian Journal of Natural Products and Resources | Year: 2012

Multi-antibiotic resistant strain of Xanthomonas campestris affects large numbers of vegetable crops and causes severe damage. To control this harmful bacterium some south Indian spices were used herbal treatment in biocontrol method. The phyto-pathogen was tested against fifteen spices: Cardamom, Turmeric, Ginger, Cumin, Fennel, Clove, Poppy seed, Mustard, Garlic, Pepper, Fenugreek, Cinnamon, Mint, Asafoetida and Coriander. The antibacterial activities of the extracts, individually and in combination with them were determined using well diffusion method and the minimum inhibitory concentration (MIC) carried out by serial dilution technique. Synergistic activity of crude aqueous and crude methanolic extracts of the plant parts, in various combinations of two to six against the test organism ranged from 10-30 mm zone of growth inhibition. Relative Percentage of Inhibition (RPI) was also calculated using standard formula. The antibacterial efficacy of the mixture of extracts from spices increased considerably when compared to the low activities recorded with the extract of individual plant parts (P<0.05). Aqueous extracts of each plant material and mixture produced greater antimicrobial activity than the methanolic extracts. The plant materials possessed antimicrobial activity with greater efficacy when used synergistically on the test organism.

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