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Hussain M.,Maize Research Institute Zemun Polje | Shah K.N.,Pmas Arid Agriculture University | Ghafoor A.,Plant Genetic Resources Institute PGRI | Kiani T.T.,Maize Research Institute Zemun Polje | Mahmood T.,Pmas Arid Agriculture University
Journal of Animal and Plant Sciences | Year: 2014

A genetic analysis study was carried out for various morphological traits in a complete 8 × 8 diallel cross of maize inbred lines under normal irrigation and drought conditions. Estimation of genetic components of variation and graphical presentation deduced that most of the traits like days to pollen shed, anthesis-silking interval, ear height, kernel rows per ear, 100-kernel weight, shelling percentage, grain yield per plant showed over-dominance type of inheritance under both normal and drought conditions unlike leaf rolling which showed partial dominance under normal but over-dominance type of inheritance under drought conditions. It can be inferred that because of over-dominance nature of inheritance of most of the yield related traits, heterosis breeding can be pursued to exploit high yielding hybrids with considerable drought tolerance. Source


Qamar M.,University of | Qamar M.,Pakistan National Agricultural Research Center | Dilnawaz Ahmad S.,University of | Ashiq Rabbani M.,Plant Genetic Resources Institute PGRI | And 2 more authors.
Pakistan Journal of Botany | Year: 2014

Stripe and leaf rusts are the major constraints to bread wheat production in Pakistan. Molecular markers were used to investigate the presence of leaf rust and stripe rust resistance gene cluster Lr34/Yr18 and stem rust resistance gene Sr2 in 52 Pakistani bread wheat cultivars/lines. PCR amplification of DNA fragments using DNA marker csLV-34 showed that 13 of the studied cultivars/lines, namely '03FJ26', 'NR 337', 'NR 339' 'NR 347', 'NR 350', 'Manthar', 'Margalla 99', 'Iqbal 2000', 'Saleem 2000', 'Wafaq 2001', 'Marwat 2001', 'Pirsabak 2004' and 'Fareed 2006' carry leaf rust and stripe rust resistance genes Lr34/Yr18. Stem rust resistance gene Sr2 was observed in 36 Pakistani spring wheat cultivars/lines using stm560.3tgag marker. The slow rusting gene Sr2 needs to be combined with additional stem rust resistance genes to establish durable resistance against Ug99 in modern wheat cultivars. Low frequency of Lr34/Yr18 was found in Pakistani wheats. This gene cluster needs to be incorporated into Pakistani wheats for durable rust resistance. Source


Iqbal J.,Quaid-i-Azam University | Shinwari Z.K.,Quaid-i-Azam University | Rabbani M.A.,Plant Genetic Resources Institute PGRI
Pakistan Journal of Botany | Year: 2015

Broad genetic makeup is always less vulnerable to various biotic and abiotic stresses. To identify and maintain diverse genetic base, the screening of collections is carried out through different marker systems. In the current investigation a total of 153 maize genotypes including 150 accessions from China, Japan and Pakistan, and 3 check varieties were characterized for 34 agronomic and morphological traits. These traits were comprised of ten qualitative and twenty-four quantitative variables which were recorded using augmented design at Plant Genetic Resources Institute (PGRI), National Agricultural Research Center (NARC) Islamabad, Pakistan during spring-2012. Descriptive statistical analysis of the data reflected that maximum variance of 3394.8 was detected in grain yield per plant followed by 1243.3 noted in plant height. The multivariate analyses showed the maximum Euclidean distance of 13.9 was noted between 15329 and 14909, 15329 and 14959 and 24690 and 14959. Cluster analysis distributed the whole genotypes into 5 clusters indicating their broad genetic base. Principal component analysis revealed that 7 of the principal components with an Eigenvalue of more than 1 accounted for 70.56 percent of the overall variations. The identification of high level of genetic diversity during the current study could be implied for maize germplasm characterization, conservation and further improvement in maize breeding. © 2015, Pakistan Botanical Society. All rights Reserved. Source


Iqbal J.,Quaid-i-Azam University | Shinwari Z.K.,Quaid-i-Azam University | Rabbani M.A.,Plant Genetic Resources Institute PGRI | Ali Khan S.,Plant Genetic Resources Institute PGRI
Pakistan Journal of Botany | Year: 2015

Agro-morphological characterization of germplasm is equally vital for the accurate conservation and successful breeding programs. During the present investigation the magnitude of genetic divergence was elucidated among 150 collections of maize of Chinese, Japanese and Pakistani origin. Three commercial varieties named Agaiti-2002, Sadaf and Sahiwal-2002 were used as check for comparison. All genotypes were evaluated for 24 quantitative and 10 qualitative agro- morphological traits under open field conditions at Plant Genetic Resources Institute (PGRI), National Agricultural Research Center (NARC) Islamabad, Pakistan during spring-2011. Basic statistical analysis showed maximum variability in grain yield per plant, plant height, 1000 kernel weight, ear height, leaf length and days to harvest among the tested genotypes. Simple correlation coefficient portrayed that some functionally related variables were significantly correlated. Cluster analysis of the data reflected high level of genetic divergence for most of the agro-morphological traits among the studied accessions such as maximum Euclidean genetic distance of 17.8 was observed between the accession 15232 and 15014 followed by 17.7 between 15329 and 15232. Accessions collected from the province of Khyber Pakhtunkhwa were noted with the highest genetic diversity followed by the accessions from Punjab, Baluchistan and Northern Areas. Principal component analysis based on quantitative agro-morphological traits further strengthened these findings. The present investigation revealed valuable amount of genetic variability among the tested maize genotypes which could be cashed for the maximum by further utilization in maize breeding strategies as a source of broad genetic base. © 2015, Pakistan Botanical Society. All rights Reserved. Source

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