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Islamabad, Pakistan

Shinwari Z.K.,Quaid-i-Azam University | Rehman H.,Quaid-i-Azam University | Rabbani M.A.,Plant Genetic Resources Institute
Pakistan Journal of Botany | Year: 2014

Safflower (Carthamus tinctorius L.) germplasm, comprising of 116 accessions was characterized using Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE) of seed storage proteins. The germplasm was acquired from different countries of the world. Total seed proteins were separated through electrophoresis polyacrylamide gels using standard protocols. Eighteen (60%) of the protein bands detected were polymorphic, the rest being monomorphic. Eight bands (14, 17, 18, 19, 20, 23, 24 and 25) were more than 80% common in all accessions. Similarity coefficients among the accessions ranged from 0.00 to 1.00. Accessions 16327 and 26752 were the most divergent genotypes having maximum dissimilarity with all the other accessions used. Unweighted Pair Group Method with Arithmetic averages (UPGMA) was used which is based on dissimilarity matrix. The dendrogram obtained separated all accessions into four main clusters (I, II, III and IV) and two independent individual genotypes. Four major clusters comprised of 23, 75, 8 and 8 accessions, respectively. This technique did not reveal genetic variability of significant value in safflower genotypes, hence advanced molecular and biochemical markers are recommended for further studies. This study will be helpful for the future breeding program of safflower accessions. Source

Saleem M.,Pakistan National Agricultural Research Center | Maqsood M.,University of Agriculture at Faisalabad | Javaid A.,Plant Genetic Resources Institute | Ul-Hassan M.,Nuclear Institute for Agriculture and Biology NIAB | Khaliq T.,University of Agriculture at Faisalabad
Pakistan Journal of Botany | Year: 2010

The objective was to find out the impact of different irrigation schedules and integrated plant nutrition levels on the seasonal crop growth rate (CGR) and net assimilation rate (NAR) of cotton. The experiments were conducted using RCBD with split plot arrangement. The treatments were four irrigation schedules (main plot) i.e. six irrigations (I1), three irrigations (I2), irrigation at 25 mm deficit (I3) and irrigation at 50 mm deficit (I4), and seven integrated nutrition levels (sub plot) viz. control (N0), 75-37.5-37.5 kg N-P2O5-K2O ha-1 (N1), 75-37.5-37.5 kg N-P2O5-K2O ha-1 + FYM @ 20 t ha-1 (N2), 75-37.5-37.5 kg N-P2O5-K2O ha-1 + wheat straw @ 5 t ha-1 (N3), 150-75-75 kg NP2O5- K2O ha-1 (N4), 150-75-75 kg N-P2O5-K2O ha-1 + FYM @ 20 t ha-1 (N5), 150-75-75 kg NP2O5- K2O ha-1 + wheat straw @ 5 t ha-1 (N6). Significantly higher seasonal CGR and NAR were attained at N5 with any of two irrigation schedules viz., I3 or I4. Interaction I3N5 increased CGR and NAR by 145% and 20% in 2003 and 146% and 18%, respectively in 2004, however, I4N5 performed at par with I3N5 in 2003. Linear relationships between CGR and seed cotton yield; and NAR and yield were drawn. Subsequently, I3N5 and I4N5 resulted in better performance and higher seed cotton yield. Source

Iqbal J.,Quaid-i-Azam University | Iqbal J.,Plant Genetic Resources Institute | Shinwari Z.K.,Quaid-i-Azam University | Rabbani M.A.,Plant Genetic Resources Institute
Pakistan Journal of Botany | Year: 2014

The assessment of genetic diversity among the members of a species is of vital importance for successful breeding and adaptability. In the present study 83 genotypes of maize of Pakistani and Japanese origin were evaluated for the total seed storage proteins using sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) through vertical slab unit. The total protein subunits were separated on 12% polyacrylamide gel using standard protocols. A total of 18 protein subunits were noted out of which 7 (39%) were monomorphic and 11 (61%) were polymorphic, with molecular weight ranging from 10 to 122 kDa. Coefficients of similarity among the accessions ranged between 0.89 and 1.00. The dendrogram obtained through UPGMA clustering method showed two main clusters: 1 and 2. First cluster comprised of 9 genotypes including Sahiwal-2002, while second cluster contained 74 genotypes including Aaiti-2002 and Sadaf. Over all a low level of polymorphism was observed in total seed storage protein patterns of maize genotypes from Pakistan as well as Japan. It is inferred from the present study that more genotypes of maize could be brought under study and more advanced biochemical techniques with more reliable results could be followed to bring assessment of genetic diversity of maize for planning breeding programs. Source

George S.,Plant Genetic Resources Institute | Ahmad Jatoi S.,Plant Genetic Resources Institute | Uddin Siddiqui S.,Plant Genetic Resources Institute
Pakistan Journal of Botany | Year: 2013

Current study describes the effect of artificial drought stress using PEG on different tomato varieties at seedling stage. The performance was assessed by germination, root and shoot length, and seedling biomass. The tomato varieties investigated have shown varying responses for different plant attributes against drought stress which remained significantly different for most of the parameters studied. For a given genotype, the effect of PEG was significant for root length and seedling biomass, whereas it was non-significant in case of germination and shoot length. The genotypic differences among tomato varieties investigated were found significant for all the plant parameters studied. Three different types of genotypic behaviors were observed in this study. The genotypes displayed either enhanced or declined growth in case of root, shoot and seedling biomass representing the two behavior types. Whereas, only in case of germination, in addition it also showed third type of behavior and remained indifferent to control and induced drought stress. Among others Walter, Punjab Chuhara & Kurihara have shown enhanced growth for all the parameters when subjected to PEG as compared to control. Money maker was the only genotype where a decline for all the attributes was recorded against drought stress, reflecting its lower tolerance. Source

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