Torres J.M.,Plant Biotechnology Unit UNALMED CIB Corporacion Para Investigaciones Biologicas CIB |
Calderon H.,Plant Biotechnology Unit UNALMED CIB Corporacion Para Investigaciones Biologicas CIB |
Rodriguez-Arango E.,Plant Biotechnology Unit UNALMED CIB Corporacion Para Investigaciones Biologicas CIB |
Morales J.G.,National University of Colombia |
And 2 more authors.
European Journal of Plant Pathology | Year: 2012
Black leaf streak or "black Sigatoka" is one of the most important diseases affecting bananas and plantains worldwide. Very few studies have been published on the host-pathogen interaction of this pathosystem, particularly at the molecular level. The aim of this work was to analyze, under controlled conditions, the enzyme activity of peroxidase (POX), phenylalanine ammonia lyase (PAL), β-1, 3-glucanase (GLU) and chitinase (CHI) as well as the production of H 2O 2 in banana plants infected with Mycosphaerella fijiensis. Defence responses were examined and compared in a resistant (Calcutta 4) and a susceptible (Williams) cultivar. Plants were inoculated and tested for enzyme activity at 0, 6, 12, 18, 24, 48 and 72 h after infection (HAI) and 6, 9, 12, 15 and 18 days after inoculation (DAI). A rapid induction of PAL, POX and GLU was observed in the resistant cultivar at 6-18 HAI as well as H 2O 2 production at 72 HAI. In contrast, in the susceptible cultivar, induction of these enzymes was only observed from 6 DAI. These results suggest that the first 72 HAI are important in determining the response of the host to the disease. Further studies characterizing banana responses to M. fijiensis at the early stages of the infection are necessary in order to better understand this host-pathogen interaction. © 2012 KNPV.