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Mouloud G.,Laboratory of Applied Microbiology | Mouloud G.,Laboratory of Plant Biotechnology | Daoud H.,Laboratory of Applied Microbiology | Bassem J.,Instituto Valenciano Of Investigaciones Agrarias | And 2 more authors.
Applied Biochemistry and Biotechnology | Year: 2013

A bacteriocin-producing strain (9,000 AU/ml) was isolated from the rhizosphere of Algerian healthy plants Ononis angustissima Lam. and identified as Bacillus clausii strain GM17. The bacteriocin, called Bac-GM17, was purified fromthe culture supernatant after heat treatment, ammonium sulfate precipitation, Sephadex G-50 chromatography andMono Q fast-performance liquid chromatography (FPLC). Based on matrix-assisted laser desorption ionization-time of flight mass spectrometry analysis, the purified Bac-GM17 is amonomer protein with a molecular mass of 5,158.11 Da. The N-terminal sequencing allowed for the straightforward identification of its first 20 residues, which were of pure bacteriocin. It also revealed that this bacteriocin contained a unique sequence, namely DWTCSKWSCLVCDDCSVELT, which suggests the identification of a novel compound. Bac-GM17 was extremely heat stable (20 min at 120 °C) and was stable within the pH range (3-9). It was found to be resistant to the proteolytic action of trypsin, pepsin, papain, pronase E, and proteinase K. It was also noted to display a bactericidal mode of action against Agrobacterium tumefaciens C58 and a fungistatic mode of action against Candida tropicalis R2 CIP203. © Springer Science+Business Media New York 2013.


Awatef R.,Laboratory of Plant Biotechnology | Hedia H.,Laboratory of Plant Biotechnology | Sonia H.,Laboratory of Plant Biotechnology | Mohamed B.,National Institute of Applied science and Technologies INSAT
African Journal of Ecology | Year: 2013

Capparis spinosa L. is a perennial shrub and drought-resistant plant and is well adapted to Mediterranean Ecosystem. Seventeen populations of caper, belonging to different bioclimatic zones, were assessed by morphological descriptors including nine parameters of seed (seed colour, major, medium and minor axial dimension, thousand seed mass, arithmetic mean diameter, geometric mean diameter, surface area and sphericity). The data underwent an analysis of variance, principal coordinate's analysis (PCO) and hierarchical classification analysis (HCA). Results showed that the dimensions of the major, medium and minor axes varied from 2.79 to 3.94 mm, 2.30 to 3.17 mm and 1.66 to 2.36 mm, respectively. The arithmetic and geometric mean diameters ranged, respectively, from 2.66 to 3.16 mm and from 2.19 to 3.05 mm. The sphericity and surface area ranged from 0.38 to 0.40 and from 1.20 to 1.24 mm2, respectively. The thousand seed mass varied from 4.71 to 12.05 g. The analysis of variance of studied descriptors showed significant differences according to the population. Both PCO and HCA generated from Gower general similarity coefficient among all populations showed two distinct clusters each corresponding to one subspecies. Grouping of populations did not reflect bioclimatic patterns, indicating specific adaptation of population to local environments. The divergence between the two caper subspecies (C. spinosa subsp. spinosa and C. spinosa subsp. rupestris), based on morphological traits, corroborates their taxonomic status. © 2012 Blackwell Publishing Ltd.

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