Gödöllő, Hungary
Gödöllő, Hungary

Time filter

Source Type

Falagan N.,Postharvest and Refrigeration Group | Falagan N.,Institute of Plant Biotechnology | Artes F.,Postharvest and Refrigeration Group | Artes F.,Institute of Plant Biotechnology | And 6 more authors.
Journal of the Science of Food and Agriculture | Year: 2016

BACKGROUND: Biochemical and enzymatic responses to long-term regulated deficit irrigation (RDI) at harvest, during cold storage and after the retail sale period of 'Flordastar' early peaches were evaluated. Irrigation strategies were Control, and two RDI applied during post-harvest period (RDI1, severe; RDI2, moderate), based on different thresholds of maximum daily shrinkage signal intensity (RDI1, 1.4 to dry; RDI2, 1.3 to 1.6). RESULTS: Both RDI provoked stress in the plant. This meant higher antioxidant concentration [averaging 1.30±0.27g ascorbic acid equivalents (AAE) kg-1 fresh weight (FW) for control and 1.77±0.35 and 1.50±0.30g AAE kg-1 FW for RDI1 and RDI2, respectively]. Antioxidant levels decreased with storage by polyphenoloxydase action, which increased (from 0.04±0.01 U mg-1 protein to 0.32±0.08 U mg-1 protein). Vitamin C was initially higher in RDI samples (44.22±0.05g total vitamin C kg-1 FW for control vs. 46.77±0.02 and 46.27±0.03g total vitamin C kg-1 FW for RDI1 and RDI2, respectively). CONCLUSION: The way RDI was applied affected bioactive fruit composition, being catalase and dehydroascorbic acid good water stress indicators. RDI strategies can be used as field practice, allowing water savings while enhanced healthy compound content in early peaches. © 2015 Society of Chemical Industry.


PubMed | Institute of Plant Biotechnology, Postharvest and Refrigeration Group and CSIC - Center of Edafology and Applied Biology of the Segura
Type: Journal Article | Journal: Journal of the science of food and agriculture | Year: 2016

Biochemical and enzymatic responses to long-term regulated deficit irrigation (RDI) at harvest, during cold storage and after the retail sale period of Flordastar early peaches were evaluated. Irrigation strategies were Control, and two RDI applied during post-harvest period (RDI1 , severe; RDI2 , moderate), based on different thresholds of maximum daily shrinkage signal intensity (RDI1 , 1.4 to dry; RDI2 , 1.3 to 1.6).Both RDI provoked stress in the plant. This meant higher antioxidant concentration [averaging 1.300.27g ascorbic acid equivalents (AAE) kg(-1) fresh weight (FW) for control and 1.770.35 and 1.500.30g AAE kg(-1) FW for RDI1 and RDI2 , respectively]. Antioxidant levels decreased with storage by polyphenoloxydase action, which increased (from 0.040.01 U mg(-1) protein to 0.320.08 U mg(-1) protein). Vitamin C was initially higher in RDI samples (44.220.05g total vitamin C kg(-1) FW for control vs. 46.770.02 and 46.270.03g total vitamin C kg(-1) FW for RDI1 and RDI2 , respectively).The way RDI was applied affected bioactive fruit composition, being catalase and dehydroascorbic acid good water stress indicators. RDI strategies can be used as field practice, allowing water savings while enhanced healthy compound content in early peaches.


PubMed | Technical University of Cartagena, Institute of Plant Biotechnology and CSIC - Center of Edafology and Applied Biology of the Segura
Type: Journal Article | Journal: Food science and technology international = Ciencia y tecnologia de los alimentos internacional | Year: 2015

Due to the water scarcity in the Mediterranean countries, irrigation must be optimized while keeping fruit quality. The effect of deficit irrigation strategies on changes in quality parameters of the early Flordastar peaches was studied. The deficit irrigation was programmed according to signal intensity of the maximum daily trunk shrinkage; deficit irrigation plants were irrigated to maintain maximum daily trunk shrinkage signal intensity values close to 1.4 or 1.3 in the case of DI1 or DI2 plants, respectively. Results were compared to a control watered at 150% crop evapotranspiration. Fruits were stored up to 14 days at 0 and 95% Relative Humidity (RH) in air or in controlled atmosphere (controlled atmosphere; 3-4 kPa O2 and 12-14 kPa CO2), followed by a retail sale period of 4 days at 15 and 90-95% Relative Humidity in air. Weight losses were lower in controlled atmosphere stored peaches from deficit irrigation. Air-stored fruits developed a more intense red color due to a faster ripening, which was not affected by the type of watering. At harvest, deficit irrigation peaches showed higher soluble solids content, which provided a better sensory evaluation. The soluble phenolic content was initially higher (55.26 0.18 mg gallic acid equivalents/100 g fresh weight) and more stable throughout postharvest life in DI1 fruits than in those from the other irrigation treatments. Concerning vitamin C, control fruits at harvest showed higher ascorbic acid than dehydroascorbic acid content (5.43 versus 2.43 mg/100 g fresh weight, respectively), while water stressed peaches showed the opposite results. The combination of DI2 and controlled atmosphere storage allowed saving a significant amount of water and provided peaches with good overall quality, maintaining the bioactive compounds analyzed.


Baksa I.,Institute of Plant Biotechnology | Nagy T.,Institute of Plant Biotechnology | Barta E.,Institute of Plant Biotechnology | Havelda Z.,Institute of Plant Biotechnology | And 4 more authors.
BMC Genomics | Year: 2015

Background: Nicotiana benthamiana is a widely used model plant species for research on plant-pathogen interactions as well as other areas of plant science. It can be easily transformed or agroinfiltrated, therefore it is commonly used in studies requiring protein localization, interaction, or plant-based systems for protein expression and purification. To discover and characterize the miRNAs and their cleaved target mRNAs in N. benthamiana, we sequenced small RNA transcriptomes and degradomes of two N. benthamiana accessions and validated them by Northern blots. Results: We used a comprehensive molecular approach to detect and to experimentally validate N. benthamiana miRNAs and their target mRNAs from various tissues. We identified 40 conserved miRNA families and 18 novel microRNA candidates and validated their target mRNAs with a genomic scale approach. The accumulation of thirteen novel miRNAs was confirmed by Northern blot analysis. The conserved and novel miRNA targets were found to be involved in various biological processes including transcription, RNA binding, DNA modification, signal transduction, stress response and metabolic process. Among the novel miRNA targets we found the mRNA of REPRESSOR OF SILENCING (ROS1). Regulation of ROS1 by a miRNA provides a new regulatory layer to reinforce transcriptional gene silencing by a post-transcriptional repression of ROS1 activity. Conclusions: The identified conserved and novel miRNAs along with their target mRNAs also provides a tissue specific atlas of known and new miRNA expression and their cleaved target mRNAs of N. benthamiana. Thus this study will serve as a valuable resource to the plant research community that will be beneficial well into the future. © 2015 Baksa et al.


Dos-Santos N.,Technical University of Cartagena | Dos-Santos N.,Institute of Plant Biotechnology | Jimenez-Araujo A.,CSIC - Instituto de la Grasa | Rodriguez-Arcos R.,CSIC - Instituto de la Grasa | And 2 more authors.
Journal of Agricultural and Food Chemistry | Year: 2011

We characterized differences in cell wall material and polysaccharide structures, due to the quantitative trait loci associated with higher flesh firmness in a nonclimacteric near-isogenic line (NIL) SC7-2, and with the climacteric behavior of the NIL SC3-5-1, using their nonclimacteric inbred parentals, "Piel de Sapo" (PS) and PI 161375 (SC). PS was firmer and had a higher ripening index and greater hemicellulosic content than SC, with its lower wall material yield, and uronic acid, neutral sugar, cellulose and free sugar content and higher pectic content. SC3-5-1 showed lower uronic acid values, a higher soluble solid content, and similar flesh firmness to PS. SC3-5-1 yielded mainly high molecular weight polysaccharides in the imidazole-soluble fraction than PS. SC7-2 showed greater flesh firmness, a higher neutral sugar (especially galactose and mannose) and uronic acid content, together with a larger cellulose and α-cellulose residue than PS. SC7-2 also contained more polysaccharides of low molecular weight in the first pectic fraction and shifted toward higher molecular weights in the main peak of the 4 M potassium-soluble fraction compared with PS. © 2011 American Chemical Society.


Martinez-Hernandez G.B.,Technical University of Cartagena | Gomez P.A.,Institute of Plant Biotechnology | Pradas I.,IFAPA Centro del Llano | Artes F.,Technical University of Cartagena | And 3 more authors.
Postharvest Biology and Technology | Year: 2011

The effects of several UV-C pre-treatments (1.5, 4.5, 9.0 and 15kJm-2) on changes in physiological, sensory and microbial quality and health promoting bioactive compounds over 19 days at 5 and 10°C of fresh-cut Bimi® broccoli were studied. Non-irradiated samples were used as controls. Bimi® broccoli (Brassica oleracea Italica Group×Alboglabra Group) is characterised by a long stem with a small floret with a mild and sweeter flavor than conventional varieties well adapted for fresh-cut purposes. Low and moderate UV-C doses (1.5 and 4.5kJm-2) had inhibitory effects on natural microflora growth. In relation to sensory quality, all treatments resulted in a shelf-life of 19 and 13 days at 5 and 10°C respectively with the exception of 15kJ UV-Cm-2 treated samples which resulted in a shorter shelf-life. These doses immediately increased total polyphenols contents up to 25% after 19 days at 5°C compared to the initial value. All the hydroxycinnamoyl acid derivates were immediately increased after UV-C treatments, with values 4.8- and 4.5-fold higher for 4.5 and 9.0kJ UV-Cm-2 treated samples respectively over the control. Changes in phenolic compounds were highly influenced by the storage temperature throughout shelf-life. Total antioxidant activity generally followed the same pattern: the higher the UV-C doses, the higher total antioxidant capacity values. Generally, UV-C slightly reduced initial total chlorophyll content but delayed its degradation throughout shelf-life. It is concluded that a pre-treatment of 4.5kJ UV-Cm-2 is useful as a technique to improve epiphytic microbial quality and health promoting bioactive compounds of fresh-cut Bimi® broccoli. © 2011 Elsevier B.V.


PubMed | Technical University of Cartagena and Institute of Plant Biotechnology
Type: Journal Article | Journal: Journal of food science and technology | Year: 2016

Thermal processing extends the shelf life of fruit and vegetables products by inactivating microorganisms and enzymes. The effect of a pasteurization (P) treatment, 902C for 35s, provided by continuous semi-industrial microwave (MW) under different conditions (high power/short time and low power/long time) or conventional pasteurization (CP) on orange-colored smoothies and their changes throughout 45days of storage at 5C were investigated. Results indicated that vitamin C and antioxidant capacity (FRAP) in CP decreased dramatically in comparison with the unheated and MWP smoothies. On the contrary, all heating treatments increased the contents of total phenolic compounds and carotenoids. Based on the sensory quality and microbial counts, the shelf life of all those heated smoothies reached 45days. No


PubMed | Institute of Plant Biotechnology
Type: | Journal: BMC genomics | Year: 2015

Nicotiana benthamiana is a widely used model plant species for research on plant-pathogen interactions as well as other areas of plant science. It can be easily transformed or agroinfiltrated, therefore it is commonly used in studies requiring protein localization, interaction, or plant-based systems for protein expression and purification. To discover and characterize the miRNAs and their cleaved target mRNAs in N. benthamiana, we sequenced small RNA transcriptomes and degradomes of two N. benthamiana accessions and validated them by Northern blots.We used a comprehensive molecular approach to detect and to experimentally validate N. benthamiana miRNAs and their target mRNAs from various tissues. We identified 40 conserved miRNA families and 18 novel microRNA candidates and validated their target mRNAs with a genomic scale approach. The accumulation of thirteen novel miRNAs was confirmed by Northern blot analysis. The conserved and novel miRNA targets were found to be involved in various biological processes including transcription, RNA binding, DNA modification, signal transduction, stress response and metabolic process. Among the novel miRNA targets we found the mRNA of REPRESSOR OF SILENCING (ROS1). Regulation of ROS1 by a miRNA provides a new regulatory layer to reinforce transcriptional gene silencing by a post-transcriptional repression of ROS1 activity.The identified conserved and novel miRNAs along with their target mRNAs also provides a tissue specific atlas of known and new miRNA expression and their cleaved target mRNAs of N. benthamiana. Thus this study will serve as a valuable resource to the plant research community that will be beneficial well into the future.


PubMed | Technical University of Cartagena, Institute of Plant Biotechnology and University of Tehran
Type: | Journal: Journal of the science of food and agriculture | Year: 2016

Thermal processing causes a number of undesirable changes in physicochemical and bioactive properties of tomato products. Microwave (MW) technology is an emergent thermal industrial process that offers a rapid and uniform heating, high energy efficiency and high overall quality of the final product. The main quality changes of tomato puree after pasteurization at 96 2 C for 35 s, provided by a semi-industrial continuous microwave oven (MWP) under different doses (low power/long time to high power/short time) or by conventional method (CP) were studied.All heat treatments reduced colour quality, total antioxidant capacity and vitamin C, with a greater reduction in CP than in MWP. On the other hand, use of an MWP, in particular high power/short time (1900 W/180 s, 2700 W/160 s and 3150 W/150 s) enhanced the viscosity and lycopene extraction and decreased the enzyme residual activity better than with CP samples. For tomato puree, polygalacturonase was the more thermo-resistant enzyme, and could be used as an indicator of pasteurization efficiency.MWP was an excellent pasteurization technique that provided tomato puree with improved nutritional quality, reducing process times compared to the standard pasteurization process. 2016 Society of Chemical Industry.


PubMed | Technical University of Cartagena, Institute of Plant Biotechnology and University of Tehran
Type: Journal Article | Journal: Food science and technology international = Ciencia y tecnologia de los alimentos internacional | Year: 2016

The effect of a pasteurization treatment at 902 for 35s provided by continuous microwave under different doses (low power/long time and high power/short time) or conventional pasteurization on the quality of orange-colored smoothies and their changes throughout 45 days of storage at 5 was investigated. A better color retention of the microwave pasteurization- treated smoothie using high power/short time than in conventionally processed sample was evidenced by the stability of the hue angle. The continuous microwave heating increased the viscosity of the smoothie more than the conventional pasteurization in comparison with non-treated samples. Lower residual enzyme activities from peroxidase, pectin methylesterase and polygalacturonase were obtained under microwave heating, specifically due to the use of higher power/shorter time. For this kind of smoothie, polygalacturonase was the more thermo-resistant enzyme and could be used as an indicator of pasteurization efficiency. The use of a continuous semi-industrial microwave using higher power and shorter time, such as 1600W/206s and 3600W/93s, resulted in better quality smoothies and greater enzyme reduction than conventional thermal treatment.

Loading Institute of Plant Biotechnology collaborators
Loading Institute of Plant Biotechnology collaborators