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Gupta S.,Rajasthan Agricultural University | Gupta N.K.,Rajasthan Agricultural University | Gupta N.K.,Plant Biotechnology Center
Indian Journal of Agricultural Sciences | Year: 2011

A field experiment was conducted during winter (rabi) season of 2005-06 and 2006-07 taking wheat (Triticum aestivum L. emend Fiori. & Paol.) genotype 'HD 2329' to investigate the efficacy of putrescine under water stress condition. Putrescine (0.01, 0.1 and 1.0 mM concentrations) was applied as seed treatment, one or two foliar sprays. For water stress treatment, the number of irrigations was reduced to three as compared to six in control. Putrescine application enhanced plant height, leaf area, grain number, grain weight, grain yield and biological yield under non-stress as well as under water stress conditions. Exogenous application of 0.1 mM putrescine was found the best in most of the observations. Among mode of applications, two foliar sprays of putrescine at the time of anthesis and post anthesis performed best over seed treatment and one foliar spray of putrescine. Source

Calingacion M.,International Rice Research Institute | Calingacion M.,Wageningen University | Calingacion M.,A+ Network | Laborte A.,International Rice Research Institute | And 92 more authors.
PLoS ONE | Year: 2014

With the ever-increasing global demand for high quality rice in both local production regions and with Western consumers, we have a strong desire to understand better the importance of the different traits that make up the quality of the rice grain and obtain a full picture of rice quality demographics. Rice is by no means a 'one size fits all' crop. Regional preferences are not only striking, they drive the market and hence are of major economic importance in any rice breeding / improvement strategy. In this analysis, we have engaged local experts across the world to perform a full assessment of all the major rice quality trait characteristics and importantly, to determine how these are combined in the most preferred varieties for each of their regions. Physical as well as biochemical characteristics have been monitored and this has resulted in the identification of no less than 18 quality trait combinations. This complexity immediately reveals the extent of the specificity of consumer preference. Nevertheless, further assessment of these combinations at the variety level reveals that several groups still comprise varieties which consumers can readily identify as being different. This emphasises the shortcomings in the current tools we have available to assess rice quality and raises the issue of how we might correct for this in the future. Only with additional tools and research will we be able to define directed strategies for rice breeding which are able to combine important agronomic features with the demands of local consumers for specific quality attributes and hence, design new, improved crop varieties which will be awarded success in the global market. Source

Dell'Acqua M.,SantAnna School of Advanced Studies | Gatti D.M.,The Jackson Laboratory | Pea G.,SantAnna School of Advanced Studies | Pea G.,Thermo Fisher Scientific | And 18 more authors.
Genome Biology | Year: 2015

Background: Maize (Zea mays) is a globally produced crop with broad genetic and phenotypic variation. New tools that improve our understanding of the genetic basis of quantitative traits are needed to guide predictive crop breeding. We have produced the first balanced multi-parental population in maize, a tool that provides high diversity and dense recombination events to allow routine quantitative trait loci (QTL) mapping in maize. Results: We produced 1,636 MAGIC maize recombinant inbred lines derived from eight genetically diverse founder lines. The characterization of 529 MAGIC maize lines shows that the population is a balanced, evenly differentiated mosaic of the eight founders, with mapping power and resolution strengthened by high minor allele frequencies and a fast decay of linkage disequilibrium. We show how MAGIC maize may find strong candidate genes by incorporating genome sequencing and transcriptomics data. We discuss three QTL for grain yield and three for flowering time, reporting candidate genes. Power simulations show that subsets of MAGIC maize might achieve high-power and high-definition QTL mapping. Conclusions: We demonstrate MAGIC maize's value in identifying the genetic bases of complex traits of agronomic relevance. The design of MAGIC maize allows the accumulation of sequencing and transcriptomics layers to guide the identification of candidate genes for a number of maize traits at different developmental stages. The characterization of the full MAGIC maize population will lead to higher power and definition in QTL mapping, and lay the basis for improved understanding of maize phenotypes, heterosis included. MAGIC maize is available to researchers. © 2015 Dell'Acqua et al. Source

Oo K.S.,Yezin | Oo K.S.,National Science and Technology Development Agency | Oo K.S.,Kasetsart University | Khanthong S.,National Science and Technology Development Agency | And 10 more authors.
Rice Science | Year: 2015

Paw San Hmwe (PSM) rice has been cultivated in many areas of Myanmar for a long time. Strong aroma, good taste and its elongation during cooking are the key characteristics of PSM rice. Thirty-one PSM accessions were genotypically characterized, and their physical grain and cooking quality traits were studied. We used specific gene markers associated with aroma, apparent amylose content (AAC) and alkali spreading value to determine the alleles carried by different PSM accessions. The results revealed that six PSM accessions (PSM10, PSM12, PSM13, PSM21, PSM22 and PSM30) had a 3-bp insertion in Os2AP gene. Gel consistency (GC) allele was predominant among the PSM accessions for gelatinization temperature (GT), however, the phenotype observed was between low and intermediate GT because of the combination of the GC allele with the presence of low GT allele at heterozygous state from the other loci of the SSIIa gene. Intermediate to high AAC was observed among the PSM accessions corresponding to the haplotype identified for the single nucleotide polymorphism G/T and the (CT)n repeat in the Wx gene. The characterization and grouping data of PSM accessions posted benefits to Myanmar seed banks, and our results will help in maintaining the integrity of PSM rice variety. © 2015 China National Rice Research Institute. Hosting by Elsevier B.V. Source

Sharma N.,Indian Agricultural Research Institute | Singh G.,Plant Biotechnology Center | Mishra M.,Central Institute of Subtropical Horticulture
Biopesticides International | Year: 2014

In this study, 32 bacteria were isolated from pearl millet, Pennisetum glaucum (L.) R. Br. rhizosphere and associated nonrhizospheric areas. Pearl millet rhizosphere and nonrhizosphere areas were dominated by gram negative bacterial population with rod shaped creamy colonies. Salinity and temperature tolerance study of bacterial isolates showed that most of the bacterial isolates (rhizospheric as well as non-rhizospheric) were less halophilic. Seed inoculation with pearl millet rhizospheric bacteria significantly enhanced seed germination and seedling vigor of pearl millet. The overall findings indicated that the most effective bacterial isolate with maximum PGPR activity is PM-14 that was confirmed by 16S rDNA gene sequencing method and BLAST analysis of the sequence revealed that the predominant microorganism is the Acetinobacter sp. The GenBank accession number of PM-14 is BankIt1541513 Seq1 JX124708. © 2014 (KRF). Source

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