Plant Biotechnology Center

Plant, India

Plant Biotechnology Center

Plant, India

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Myint K.M.,Plant Biotechnology Center | Myint K.M.,Kasetsart University | Courtois B.,CIRAD - Agricultural Research for Development | Risterucci A.-M.,CIRAD - Agricultural Research for Development | And 5 more authors.
Rice | Year: 2012

Background: After observing peculiar rice varieties in Myanmar, in terms of classification in varietal groups and of grain quality, we focused on Myanmar varieties and analyzed variations at 19 microsatellite loci as well as sequences of the aroma gene BADH2. Results: Microsatellites were able to retrieve the well-established classification into Indica (isozyme group 1), Japonica (group 6, comprising temperate and tropical forms) and specific groups from the Himalayan foothills including some Aus varieties (group 2) and some aromatic varieties (group 5). They revealed a new cluster of accessions close to, but distinct from, non- Myanmar varieties in group 5. With reference to earlier terminology, we propose to distinguish a group "5A" including group 5 varieties from the Indian subcontinent (South and West Asia) and a group "5B" including most group 5 varieties from Myanmar. In Myanmar varieties, aroma was distributed in group 1 (Indica) and in group 5B. New BADH2 variants were found. Some accessions carried a 43 bp deletion in the 3' UTR that was not completely associated with aroma. Other accessions, all of group 5B, displayed a particular BADH2 allele with a 3 bp insertion and 100% association with aroma. Conclusion: With the new group and the new alleles found in Myanmar varieties, our study shows that the Himalayan foothills contain series of non-Indica and non-Japonica varietal types with novel variations for useful traits. © 2012 Myint et al.


Gupta S.,Rajasthan Agricultural University | Gupta N.K.,Rajasthan Agricultural University | Gupta N.K.,Plant Biotechnology Center
Indian Journal of Agricultural Sciences | Year: 2011

A field experiment was conducted during winter (rabi) season of 2005-06 and 2006-07 taking wheat (Triticum aestivum L. emend Fiori. & Paol.) genotype 'HD 2329' to investigate the efficacy of putrescine under water stress condition. Putrescine (0.01, 0.1 and 1.0 mM concentrations) was applied as seed treatment, one or two foliar sprays. For water stress treatment, the number of irrigations was reduced to three as compared to six in control. Putrescine application enhanced plant height, leaf area, grain number, grain weight, grain yield and biological yield under non-stress as well as under water stress conditions. Exogenous application of 0.1 mM putrescine was found the best in most of the observations. Among mode of applications, two foliar sprays of putrescine at the time of anthesis and post anthesis performed best over seed treatment and one foliar spray of putrescine.


Sawardekar S.V.,Plant Biotechnology Center | Mhatre N.K.,Plant Biotechnology Center | Sawant S.S.,Plant Biotechnology Center | Bhave S.G.,Plant Biotechnology Center | And 4 more authors.
Indian Journal of Genetics and Plant Breeding | Year: 2012

Pod borer is a serious pest of pigeonpea. It was observed that borer infected flower buds do not produce any seed but only pods. In the present investigation, successful transfer and expression of cryIIAa gene was made through Agrobacterium into a popular pigeonpea variety, Konkan Tur-1. of pigeonpea through -mediated gene transfer method. The ability to regenerate in vitro as well use of positive selection system may provide means for recovery of transgenic pigeonpea.


Oo K.S.,Yezin | Oo K.S.,National Science and Technology Development Agency | Oo K.S.,Kasetsart University | Khanthong S.,National Science and Technology Development Agency | And 10 more authors.
Rice Science | Year: 2015

Paw San Hmwe (PSM) rice has been cultivated in many areas of Myanmar for a long time. Strong aroma, good taste and its elongation during cooking are the key characteristics of PSM rice. Thirty-one PSM accessions were genotypically characterized, and their physical grain and cooking quality traits were studied. We used specific gene markers associated with aroma, apparent amylose content (AAC) and alkali spreading value to determine the alleles carried by different PSM accessions. The results revealed that six PSM accessions (PSM10, PSM12, PSM13, PSM21, PSM22 and PSM30) had a 3-bp insertion in Os2AP gene. Gel consistency (GC) allele was predominant among the PSM accessions for gelatinization temperature (GT), however, the phenotype observed was between low and intermediate GT because of the combination of the GC allele with the presence of low GT allele at heterozygous state from the other loci of the SSIIa gene. Intermediate to high AAC was observed among the PSM accessions corresponding to the haplotype identified for the single nucleotide polymorphism G/T and the (CT)n repeat in the Wx gene. The characterization and grouping data of PSM accessions posted benefits to Myanmar seed banks, and our results will help in maintaining the integrity of PSM rice variety. © 2015 China National Rice Research Institute. Hosting by Elsevier B.V.


Dell'Acqua M.,Sant'Anna School of Advanced Studies | Gatti D.M.,The Jackson Laboratory | Pea G.,Sant'Anna School of Advanced Studies | Pea G.,Thermo Fisher Scientific | And 18 more authors.
Genome Biology | Year: 2015

Background: Maize (Zea mays) is a globally produced crop with broad genetic and phenotypic variation. New tools that improve our understanding of the genetic basis of quantitative traits are needed to guide predictive crop breeding. We have produced the first balanced multi-parental population in maize, a tool that provides high diversity and dense recombination events to allow routine quantitative trait loci (QTL) mapping in maize. Results: We produced 1,636 MAGIC maize recombinant inbred lines derived from eight genetically diverse founder lines. The characterization of 529 MAGIC maize lines shows that the population is a balanced, evenly differentiated mosaic of the eight founders, with mapping power and resolution strengthened by high minor allele frequencies and a fast decay of linkage disequilibrium. We show how MAGIC maize may find strong candidate genes by incorporating genome sequencing and transcriptomics data. We discuss three QTL for grain yield and three for flowering time, reporting candidate genes. Power simulations show that subsets of MAGIC maize might achieve high-power and high-definition QTL mapping. Conclusions: We demonstrate MAGIC maize's value in identifying the genetic bases of complex traits of agronomic relevance. The design of MAGIC maize allows the accumulation of sequencing and transcriptomics layers to guide the identification of candidate genes for a number of maize traits at different developmental stages. The characterization of the full MAGIC maize population will lead to higher power and definition in QTL mapping, and lay the basis for improved understanding of maize phenotypes, heterosis included. MAGIC maize is available to researchers. © 2015 Dell'Acqua et al.


Sharma N.,Indian Agricultural Research Institute | Singh G.,Plant Biotechnology Center | Mishra M.,Central Institute of Subtropical Horticulture
Biopesticides International | Year: 2014

In this study, 32 bacteria were isolated from pearl millet, Pennisetum glaucum (L.) R. Br. rhizosphere and associated nonrhizospheric areas. Pearl millet rhizosphere and nonrhizosphere areas were dominated by gram negative bacterial population with rod shaped creamy colonies. Salinity and temperature tolerance study of bacterial isolates showed that most of the bacterial isolates (rhizospheric as well as non-rhizospheric) were less halophilic. Seed inoculation with pearl millet rhizospheric bacteria significantly enhanced seed germination and seedling vigor of pearl millet. The overall findings indicated that the most effective bacterial isolate with maximum PGPR activity is PM-14 that was confirmed by 16S rDNA gene sequencing method and BLAST analysis of the sequence revealed that the predominant microorganism is the Acetinobacter sp. The GenBank accession number of PM-14 is BankIt1541513 Seq1 JX124708. © 2014 (KRF).


PubMed | Yezin, Kasetsart University, CIRAD - Agricultural Research for Development and Plant Biotechnology Center
Type: Journal Article | Journal: Rice (New York, N.Y.) | Year: 2016

After observing peculiar rice varieties in Myanmar, in terms of classification in varietal groups and of grain quality, we focused on Myanmar varieties and analyzed variations at 19 microsatellite loci as well as sequences of the aroma gene BADH2.Microsatellites were able to retrieve the well-established classification into Indica (isozyme group 1), Japonica (group 6, comprising temperate and tropical forms) and specific groups from the Himalayan foothills including some Aus varieties (group 2) and some aromatic varieties (group 5). They revealed a new cluster of accessions close to, but distinct from, non-Myanmar varieties in group 5. With reference to earlier terminology, we propose to distinguish a group 5A including group 5 varieties from the Indian subcontinent (South and West Asia) and a group 5B including most group 5 varieties from Myanmar. In Myanmar varieties, aroma was distributed in group 1 (Indica) and in group 5B. New BADH2 variants were found. Some accessions carried a 43bp deletion in the 3 UTR that was not completely associated with aroma. Other accessions, all of group 5B, displayed a particular BADH2 allele with a 3bp insertion and 100% association with aroma.With the new group and the new alleles found in Myanmar varieties, our study shows that the Himalayan foothills contain series of non-Indica and non-Japonica varietal types with novel variations for useful traits.


Calingacion M.,International Rice Research Institute | Calingacion M.,Wageningen University | Laborte A.,International Rice Research Institute | Nelson A.,International Rice Research Institute | And 48 more authors.
PLoS ONE | Year: 2014

With the ever-increasing global demand for high quality rice in both local production regions and with Western consumers, we have a strong desire to understand better the importance of the different traits that make up the quality of the rice grain and obtain a full picture of rice quality demographics. Rice is by no means a 'one size fits all' crop. Regional preferences are not only striking, they drive the market and hence are of major economic importance in any rice breeding / improvement strategy. In this analysis, we have engaged local experts across the world to perform a full assessment of all the major rice quality trait characteristics and importantly, to determine how these are combined in the most preferred varieties for each of their regions. Physical as well as biochemical characteristics have been monitored and this has resulted in the identification of no less than 18 quality trait combinations. This complexity immediately reveals the extent of the specificity of consumer preference. Nevertheless, further assessment of these combinations at the variety level reveals that several groups still comprise varieties which consumers can readily identify as being different. This emphasises the shortcomings in the current tools we have available to assess rice quality and raises the issue of how we might correct for this in the future. Only with additional tools and research will we be able to define directed strategies for rice breeding which are able to combine important agronomic features with the demands of local consumers for specific quality attributes and hence, design new, improved crop varieties which will be awarded success in the global market.


Narayan S.,Plant Biotechnology Center | Saxena S.N.,Plant Biotechnology Center | Jakhar M.L.,Plant Biotechnology Center | Sharma R.,Plant Biotechnology Center
Legume Research | Year: 2015

An experiment was carried out to assess effect of gamma irradiation on morphogenetic potential of callus derived from primary leaves of seedlings of moth bean seeds. Germination percentage was decreased as the dose of gamma irradiation increased from 200 to 800 Gy. Regeneration potential of callus derived from primary leaves of the treated seeds was increased with lower doses of gamma irradiations (200 Gy and 400 Gy). Exposure to 400 Gy resulted in marked increase in number of shoot bud primordial and number of shoots per explant. Further, the plantlets regenerated from such explants produced more number of flowers under in vitro conditions. Genotype Jadia was found highly responsive to in vitro mutagenesis. © 2015, Agricultural Research Communication Centre. All Rights Reserved.

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