PLA 323 Hospital

Xi’an, China

PLA 323 Hospital

Xi’an, China
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PubMed | PLA 323 Hospital, PLA Fourth Military Medical University and Peking University
Type: Journal Article | Journal: Oncogene | Year: 2016

Cellular plasticity has an important role in the progression of hepatocellular carcinoma (HCC). In this study, the involvement of a TGF-1-CD147 self-sustaining network in the regulation of the dedifferentiation progress was fully explored in HCC cell lines, hepatocyte-specific basigin/CD147-knockout mice and human HCC tissues. We demonstrated that TGF-1 stimulation upregulated CD147 expression and mediated the dedifferentiation of HCC cells, whereas all-trans-retinoic acid induced the downregulation of CD147 and promoted differentiation in HCC cells. Overexpression of CD147 induced the dedifferentiation and enhanced the malignancy of HCC cells, and increased the transcriptional expression of TGF-1 by activating -catenin. CD147-induced matrix metalloproteinase (MMP) production activated pro-TGF-1. The activated TGF-1 signaling subsequently repressed the HNF4 expression via Smad-Snail1 signaling and enhanced the dedifferentiation progress. Hepatocyte-specific basigin/CD147-knockout mice decreased the susceptibility to N-nitrosodiethylamine-induced tumorigenesis by suppressing TGF-1-CD147 signaling and inhibiting dedifferentiation in hepatocytes during tumor progression. CD147 was positively correlated with TGF-1 and negatively correlated with HNF4 in human HCC tissues. Positive CD147 staining and lower HNF4 levels in tumor tissues were significantly associated with poor survival of patients with HCC. The overexpression of HNF4 and Smad7 and the deletion of CD147 by lentiviral vectors jointly reprogrammed the expression profile of hepatocyte markers and attenuated malignant properties including proliferation, cell survival and tumor growth of HCC cells. Our results highlight the important role of the TGF-1-CD147 self-sustaining network in driving HCC development by regulating differentiation plasticity, which provides a strong basis for further investigations of the differentiation therapy of HCC targeting TGF-1 and CD147.

Wu Y.,PLA 323 Hospital | Li F.,PLA 323 Hospital | Huyan J.,PLA 323 Hospital | Zou X.,PLA 323 Hospital | Li Y.,Xi'an University of Science and Technology
Journal of Applied Polymer Science | Year: 2015

Hybrid fillers of mono-amine polyhedral oligomeric silsesquioxane/nanosized boron nitride (NH2-POSS/n-BN) were performed to fabricate NH2-POSS/n-BN/epoxy nanocomposites. Results revealed that the dielectric constant and dielectric loss values were decreased with the increasing addition of NH2-POSS obviously, but increased with the increasing addition of BN fillers. For a given loading of NH2-POSS (5 wt %), the thermal conductivities of NH2-POSS/n-BN/epoxy nanocomposites were improved with the increasing addition of n-BN fillers, and the thermal conductivity of the nanocomposites was 1.28 W/mK with 20 wt % n-BN fillers. Meantime, the thermal stability of the NH2-POSS/n-BN/epoxy nanocomposites was also increased with the increasing addition of n-BN fillers. © 2015 Wiley Periodicals, Inc.

Wu J.,PLA Fourth Military Medical University | Li Y.,PLA Fourth Military Medical University | Li Y.,PLA 323 Hospital | Dang Y.-Z.,PLA 323 Hospital | And 3 more authors.
Molecular Cancer Therapeutics | Year: 2015

Radiotherapy has played a limited role in the treatment of hepatocellular carcinoma (HCC) due to the risk of tumor radioresistance. A previous study in our laboratory confirmed that CD147 interacts with integrin β1 and plays an important role in modulating the malignant properties of HCC cells. In this study, we further evaluated the role of CD147 in the radioresistance of HCC and as a potential target for improving radiosensitivity. Upon irradiation, the colony formation, apoptosis, cell-cycle distribution, migration, and invasion of SMMC-7721, CD147-knockout SMMC-7721, HepG2, and CD147-knockdown HepG2 cells were determined. A nude mouse xenograft model and a metastatic model of HCC were used to detect the role of CD147 in radioresistance in vivo. Deletion of HAb18G/CD147 significantly enhanced the radiosensitivity of SMMC-7721 and HepG2 cells, and knocking out HAb18G/CD147 in SMMC-7721 cells attenuated irradiation-enhanced migration and invasion. The knockout and antibody blockade of CD147 decreased the tumor growth and metastatic potentials of HCC cells under irradiation. CD147-deleted SMMC-7721 cells showed diminished levels of calpain, cleaved talin, active integrin β1, and decreased p-FAK (Tyr397) and p-Akt (Ser473) levels. FAK and PI3K inhibitors, as well as integrin β1 antibodies, increased the radiation-induced apoptosis of SMMC-7721 cells. Our data provide evidence for CD147 as an important determinant of radioresistance via the regulation of integrin β1 signaling. Inhibition of the HAb18G/CD147 integrin interaction may improve the efficiency of radiosensitivity and provide a potential new approach for HCC therapy. © 2014 AACR.

Li X.-W.,PLA Fourth Military Medical University | He H.,PLA 323 Hospital | Liu Y.-F.,PLA 451 Hospital | Gao F.,PLA Fourth Military Medical University | And 4 more authors.
European Review for Medical and Pharmacological Sciences | Year: 2012

BACKGOUND: Epilepsy is a recurrent chronic nervous system disease. A correct choice of antiepileptic drug is the key to control seizures and improve patient's life quality. AIM: To study the effectiveness and safety of lamotrigine monotherapy for treatment of epilepsy, systemic evaluation was carried out on published comparative trials between lamotrigine and carbamazepine. METHODS: The retrieval method referred to the search strategy developed by Cochrane Epilepsy Group and software Rev.Man 5 was used for META analysis and forest plots.The Odds ratio (OR) was selected as the effect size and funnel plot was used to analyze the publication bias. RESULTS: A total of 9 studies and 2269 cases of patients were included in the analysis. There was no significant difference between lamotrigine and carbamazepine for treatment of epilepsy as the OR was 1.17, 95% confidence interval (CI) [0.88, 1.54]. However, lamotrigine had advantages in the overall withdrawal rate and withdrawal rate due to side effects as the ORs were 0.57, 95% CI [0.47, 0.69]) and 0.41, 95% CI [0.32, 0.52]. CONCLUSIONS: Lamotrigine has certain advantages over carbamazepine for treatment of epilepsy as it has less side effects and higher tolerability. In addition, the quality of such clinical trials should be further improved to have a more comprehensive understanding.

PubMed | PLA 323 Hospital and PLA Fourth Military Medical University
Type: Journal Article | Journal: Acta biochimica et biophysica Sinica | Year: 2015

Excessive exposure to infrasound, a kind of low-frequency but high-intensity sound noise generated by heavy transportations and machineries, can cause vibroacoustic disease which is a progressive and systemic disease, and finally results in the dysfunction of central nervous system. Our previous studies have demonstrated that glial cell-mediated inflammation may contribute to infrasound-induced neuronal impairment, but the underlying mechanisms are not fully understood. Here, we show that cannabinoid (CB) receptors may be involved in infrasound-induced neuronal injury. After exposure to infrasound at 16 Hz and 130 dB for 1-14 days, the expression of CB receptors in rat hippocampi was gradually but significantly decreased. Their expression levels reached the minimum after 7- to 14-day exposure during which the maximum number of apoptotic cells was observed in the CA1. 2-Arachidonoylglycerol (2-AG), an endogenous agonist for CB receptors, reduced the number of infrasound-triggered apoptotic cells, which, however, could be further increased by CB receptor antagonist AM251. In animal behavior performance test, 2-AG ameliorated the infrasound-impaired learning and memory abilities of rats, whereas AM251 aggravated the infrasound-impaired learning and memory abilities of rats. Furthermore, the levels of proinflammatory cytokines tumor necrosis factor alpha and interleukin-1 in the CA1 were upregulated after infrasound exposure, which were attenuated by 2-AG but further increased by AM251. Thus, our results provide the first evidence that CB receptors may be involved in infrasound-induced neuronal impairment possibly by affecting the release of proinflammatory cytokines.

PubMed | PLA 323 Hospital and PLA Fourth Military Medical University
Type: Journal Article | Journal: Diabetologia | Year: 2016

Diabetic cardiomyopathy (DCM) is associated with suppressed autophagy and augmented apoptosis in the heart although the interplay between the two remains elusive. The ability of mammalian sterile 20-like kinase 1 to regulate both autophagy and apoptosis prompted us to investigate it as a possible candidate in the progression of DCM.Wild-type, Mst1 (also known as Stk4) transgenic and Mst1-knockout mice were challenged with streptozotocin to induce experimental diabetes. In addition, cultured neonatal mouse cardiomyocytes were subjected to simulated diabetes to probe mechanisms.Mst1 knockout alleviated while Mst1 overexpression aggravated cardiac dysfunction in diabetes. Diabetic Mst1 transgenic mice exhibited decreased LC3 expression and enhanced protein aggregation. In contrast, typical autophagosomes were observed in diabetic Mst1-knockout mice with increased LC3 expression and reduced protein aggregation. Mst1 downregulation promoted autophagic flux as demonstrated by increased LC3-II and decreased p62 expression in the presence of bafilomycin A1. Furthermore, Mst1 overexpression increased, while Mst1 knockout decreased, cardiomyocyte apoptosis both in vivo and in vitro. Co-immunoprecipitation assays showed that Mst1 overexpression promoted Beclin1 binding to B cell lymphoma 2 (Bcl-2) and induced dissociation of Bcl-2 from Bax in diabetic mice. Conversely, Mst1 knockout disrupted the Beclin1-Bcl-2 complex and enhanced the interaction between Bcl-2 and Bax.Mst1 knockout restores autophagy and protects against apoptosis in cardiomyocytes, en route to the rescue against DCM.

Ning X.,Northwestern Polytechnical University | Ning X.,University of Calgary | Wu Y.,PLA 323 Hospital | Yuan J.,Northwestern Polytechnical University | And 2 more authors.
Polymer Composites | Year: 2014

Increasingly complex space operations require novel advanced structural composites for reconfigurable spacecrafts to have excellent flexural strength in reconfiguration processing and orbital maneuvering. Functionalized multiwalled carbon nanotubes (MWCNTs) are performed to fabricate the MWCNTs/carbon fiber/N, N′-4, 40-bimaleimide diphenyl methane/diallylbisphenol A (MWCNTs/CF/BMI/DBA) composites. ANSYS analysis reveals that the requiring lowest flexural strength of the composites is 1,150 MPa to meet actual requirements. The flexural strength of the MWCNTs/CF/BMI/DBA composites with 1 wt% functionalized MWCNTs can fully meet the actual use for reconfigurable spacecrafts. And the thermal stabilities of the MWCNTs/CF/BMI/DBA composites are increased with the increasing addition of MWCNTs. © 2014 Society of Plastics Engineers.

Wang J.,Northwestern Polytechnical University | Tang L.,Northwestern Polytechnical University | Gu J.,Northwestern Polytechnical University | Yang X.,Northwestern Polytechnical University | And 2 more authors.
Optoelectronics and Advanced Materials, Rapid Communications | Year: 2016

Carboxyl powdery nitrile rubbers (CPNBRs) areblended with bisphenol A epoxy resin (E-51) to produce the corresponding CPNBRs/E-51 composites via casting method. The effects of the percentage mass of the CPNBRs on the impact strength, thermal stabilities and dielectric properties of the CPNBRs/E-51 composites are discussed and investigated. The impact strength of the resulting CPNBRs/E-51 composites is found to be optimal with 5 wt% addition of CPNBRs, enhanced from 14.2 kJ/m2to 20.8 kJ/m2, increased by 46.5%. In addition, the dielectric constant and dielectric loss values of the resulting CPNBRs/E-51 composites are increased slightly, but the thermal stabilities are decreased with the increasing addition of CPNBRs. © 2016, National Institute of Optoelectronics. All rights reserved.

Gu J.,Northwestern Polytechnical University | Lv Z.,Northwestern Polytechnical University | Wu Y.,PLA 323 Hospital | Zhao R.,Northwestern Polytechnical University | And 2 more authors.
Composites Part A: Applied Science and Manufacturing | Year: 2015

Silicon carbide particle/polystyrene (SiCp/PS) electrospun mats are firstly prepared by electrospinning technology, then to be fabricated the corresponding thermally conductive SiCp/PS composites by the method of "laminating-hot press". The mass fraction of SiCp and laminating mode of SiCp/PS electrospun mats affecting on the thermal conductivities, dielectric and thermal properties of the composites are investigated. The addition of 32.8 vol% SiCp improves the thermally conductive coefficient λ of pure PS from 0.182 to 0.566 W/m K and thermal diffusivity of pure PS from 0.169 to 0.376 mm2/s, whereas the dielectric constant values still remain at relatively low levels. The thermal stabilities of the SiCp/PS composites are increased with the increasing addition of SiCp. For a given SiCp loading, the SiCp/PS composites from warp-weft arrangement of SiCp/PS electrospun mats possess relative higher thermally conductive coefficient λ and dielectric constant values than those of SiCp/PS composites from warp-warp arrangement of SiCp/PS electrospun mats. © 2015 Elsevier Ltd.

Ma A.,Xi'an Technological University | Wu Y.,Xi'an Technological University | Wu Y.,PLA 323 Hospital | Chen W.,Xi'an Technological University | Wu X.,Xi'an Technological University
Polymer Composites | Year: 2014

Multi-walled carbon nanotubes/carbon fiber (MWCNTs/CF) hybrid fillers are employed to prepare MWCNTs/CF/epoxy composites. Results reveal that a great improvement of the thermal conductivities of the epoxy composites with the addition of MWCNTs/CF hybrid fillers, and the thermal conductivity of the MWCNTs/CF/epoxy composites is 1.426 W/mK with 8 vol% treated MWCNTs/CF hybrid fillers (5 vol% MWCNTs + 3 vol% CF). Both the flexural and impact strength of the MWCNTs/CF/epoxy composites are increased firstly, but decreased with the excessive addition of MWCNTs. The flexural and impact strength of the MWCNTs/epoxy composites are optimal with 2 vol% MWCNTs. For a given MWCNTs/CF hybrid fillers loading, the surface treatment of MWCNTs/CF hybrid fillers can further increase the thermal conductivities and mechanical properties of the MWCNTs/CF/epoxy composites. © 2014 Society of Plastics Engineers.

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