303 Hospital of PLA

Nanning, China

303 Hospital of PLA

Nanning, China
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Li Y.,The General Hospital of Jinan Military Command | Li Y.,Shanghai University | Gu X.,Shandong University | Yang C.,Shanghai University | And 2 more authors.
International Journal of Clinical and Experimental Pathology | Year: 2017

Axillary osmidrosis (AO) is a clinical condition in which apocrine glands cause strong unpleasant axillary odor. Although apolipoprotein D (ApoD) has been identified as an axillary odorant binding protein, its association with AO is largely unknown. In this study, we enrolled 78 AO patients who underwent surgical treatment in our hospital between 2012 and 2015. Sixteen skin repair male patients were used as controls. We analyzed the secretion status of apocrine glands in axillary tissues by H & E staining and compared ApoD protein expression by immunohistochemistry (IHC). When compared with controls, the apocrine glands in AO group were active with cells projecting towards the lumen, forming thicker walls and smaller chambers. Much more extensive cytoplasmic brown pigmentary granules were observed in the apocrine gland cells in AO group. The average optical density and integrated optical density (IOD) in AO group was significantly higher compared with controls (P < 0.05), suggesting a significantly higher ApoD expression in AO group. We further divided the AO patients into mild, middle and severe subgroups, and compared their expression of ApoD mRNA. We found that severe subgroup had higher mean ApoD mRNA expression (P < 0.05) and higher proportion of high ApoD expression when compared with the other subgroups (X2 = 12.06, P < 0.05), suggesting a correlation between ApoD expression and the severity of AO. Our data revealed that ApoD were highly expressed in the apocrine sweat glands in AO, which might play an important role in the pathogenesis of the disease.


Yang T.-M.,303 Hospital of PLA | Wei Q.-Z.,U.S. Food and Drug Administration | Lu W.-Z.,303 Hospital of PLA | Fan X.-L.,303 Hospital of PLA
Journal of the College of Physicians and Surgeons Pakistan | Year: 2016

Objective: To compare the ventilatory effects of the three-way laryngeal mask airway (TLMA) and tracheal tube (TT) on hemodynamics, respiratory function, and stress responses in a canine model during bronchoalveolar lavage (BAL). Study Design: Experimental study. Place and Duration of Study: The 303rd Hospital of the Chinese People's Liberation Army in May 2013. Methodology: Sixteen dogs were divided into two groups. MAP, SpO2 and HR were recorded before anesthesia (T0), immediately before intubation (T1), during intubation (T2), at 3 (T3) and 10 (T4) minutes after mechanical ventilation, at 10 (T5), 20 (T6), and 30 (T7) minutes during the course of BAL, during extubation (T8), and 3 minutes after extubation (T9). Tidal volume, peak inspiratory airway pressure, and expiratory CO2 pressure were recorded at time points T2, T5, T6, T7, and T8. Stress responses variables, including epinephrine and norepinephrine levels, were examined at time points T0, T2, T3, T5, T8, and T9. Results: BAL was successfully completed in all animals. In comparison to the TT, the TLMA was capable of maintaining hemodynamic stability and ventilation (p < 0.05), and producing less stress responses (p < 0.05). Conclusion: In a canine model, ventilation with the TLMA was better than the TT during BAL in terms of maintaining effective ventilation and stable hemodynamics, and producing less stress responses.


PubMed | U.S. Food and Drug Administration and 303 Hospital of PLA
Type: Journal Article | Journal: Journal of the College of Physicians and Surgeons--Pakistan : JCPSP | Year: 2016

To compare the ventilatory effects of the three-way laryngeal mask airway (TLMA) and tracheal tube (TT) on hemodynamics, respiratory function, and stress responses in a canine model during bronchoalveolar lavage (BAL).Experimental study.The 303rd Hospital of the Chinese People's Liberation Army in May 2013.Sixteen dogs were divided into two groups. MAP, SpO2 and HR were recorded before anesthesia (T0), immediately before intubation (T1), during intubation (T2), at 3 (T3) and 10 (T4) minutes after mechanical ventilation, at 10 (T5), 20 (T6), and 30 (T7) minutes during the course of BAL, during extubation (T8), and 3 minutes after extubation (T9). Tidal volume, peak inspiratory airway pressure, and expiratory CO2 pressure were recorded at time points T2, T5, T6, T7, and T8. Stress responses variables, including epinephrine and norepinephrine levels, were examined at time points T0, T2, T3, T5, T8, and T9.BAL was successfully completed in all animals. In comparison to the TT, the TLMA was capable of maintaining hemodynamic stability and ventilation (p < 0.05), and producing less stress responses (p < 0.05).In a canine model, ventilation with the TLMA was better than the TT during BAL in terms of maintaining effective ventilation and stable hemodynamics, and producing less stress responses.


Yang Z.-S.,Hubei University of Medicine | Tang X.-J.,Hubei University of Medicine | Guo X.-R.,Hubei University of Medicine | Zou D.-D.,Hubei University of Medicine | And 6 more authors.
OncoTargets and Therapy | Year: 2014

Background: Mesenchymal stem cells (MSCs) have been considered to hold great potential as ideal carriers for the delivery of anticancer agents since the discovery of their tumor tropism. This study was performed to demonstrate the effects of phosphatase and tensin homolog (PTEN) engineering on MSCs' capacity for cancer cell-oriented migration. Methods: MSCs were engineered with a PTEN-bearing plasmid and the expression was confirmed with Western blotting. A human glioma cell line (DBTRG) was used as the target cell; DBTRG cell-oriented migration of MSCs was monitored with a micro speed photographic system. Results: The expression of transfected PTEN in MSCs was identified by immunoblotting analysis and confirmed with cell viability assessment of target cells. The DBTRG cell-oriented migration of PTEN-engineered MSCs was demonstrated by a real-time dynamic monitoring system, and a phagocytosis-like action of MSCs was also observed. Conclusion: MSCs maintained their capacity for cancer cell-directed migration after they were engineered with anticancer genes. This study provides the first direct evidence of MSCs' tropism post-anticancer gene engineering. © 2014 Yang et al.


PubMed | Hubei University of Medicine, University of British Columbia and 303 Hospital of PLA
Type: Journal Article | Journal: Oncotarget | Year: 2016

Since the tumor-oriented homing capacity of mesenchymal stem cells (MSCs) was discovered, MSCs have attracted great interest in the research field of cancer therapy mainly focused on their use as carries for anticancer agents. Differing from DNA-based vectors, the use of mRNA-based antituor gene delivery benefits from readily transfection and mutagenesis-free. However, it is essential to verify if mRNA transfection interferes with MSCs tropism and their antitumor properties. TRAIL- and PTEN-mRNAs were synthesized and studied in an in vitro model of MSC-mediated indirect co-culture with DBTRG human glioma cells. The expression of TRAIL and PTEN in transfected MSCs was verified by immunoblotting analysis, and the migration ability of MSCs after anticancer gene transfection was demonstrated using transwell co-cultures. The viability of DBTRG cells was determined with bioluminescence, live/dead staining and real time cell analyzer. An in vivo model of DBTRG cell-derived xenografted tumors was used to verify the antitumor effects of TRAIL- and PTEN-engineered MSCs. With regard to the effect of mRNA transfection on MSCs migration toward glioma cells, an enhanced migration rate was observed with MSCs transfected with all tested mRNAs compared to non-transfected MSCs (p<0.05). TRAIL- and PTEN-mRNA-induced cytotoxicity of DBTRG glioma cells was proportionally correlated with the ratio of conditioned medium from transfected MSCs. A synergistic action of TRAIL and PTEN was demonstrated in the current co-culture model. The immunoblotting analysis revealed the apoptotic nature of the cells death in the present study. The growth of the xenografted tumors was significantly inhibited by the application of MSCPTEN or MSCTRAIL/PTEN on day 14 and MSCTRAIL on day 28 (p<0.05). The results suggested that anticancer gene-bearing mRNAs synthesized in vitro are capable of being applied for MSC-mediated anticancer modality. This study provides an experimental base for further clinical anticancer studies using synthesized mRNAs.


Tian F.,303 Hospital of PLA
Nan fang yi ke da xue xue bao = Journal of Southern Medical University | Year: 2012

To evaluate the value of CT common rail technique for application in intensity-modulated radiotherapy for nasopharyngeal carcinoma (NPC). Twenty-seven NPC patients underwent Somatom CT scans using the Siemens CTVision system prior to the commencement of the radiotherapy sessions. The acquired CT images were registered with the planning CT images using the matching function of the system to obtain the linear set-up errors of 3 directions, namely X (left to right), Y (superior to inferior), and Z (anterior to posterior). The errors were then corrected online on the moving couch. The 27 NPC patients underwent a total of 110 CT scans and the displacement deviations of the X, Y and Z directions were -0.16∓1.68 mm, 0.25∓1.66 mm, and 0.33∓1.09 mm, respectively. CT common rail technique can accurately and rapidly measure the space error between the posture and the target area to improve the set-up precision of intensity-modulated radiotherapy for NPC.


Moniri M.R.,University of British Columbia | Sun X.-Y.,303 Hospital of PLA | Rayat J.,University of British Columbia | Dai D.,University of British Columbia | And 5 more authors.
Cancer Gene Therapy | Year: 2012

Mesenchymal stem cells (MSCs) have attracted great interest in cancer therapy owing to their tumor-oriented homing capacity and the feasibility of autologous transplantation. Currently, pancreatic cancer patients face a very poor prognosis, primarily due to the lack of therapeutic strategies with an effective degree of specificity. Anticancer gene-engineered MSCs specifically target tumor sites and can produce anticancer agents locally and constantly. This study was performed to characterize pancreas-derived MSCs and investigate the effects of tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)-engineered MSCs on pancreatic cancer cells under different culture conditions. Pancreas-derived MSCs exhibited positive expression on CD44, CD73, CD95, CD105, negative on CD34 and differentiated into adipogenic and osteogenic cells. TRAIL expression was assessed by both enzyme-linked immunosorbent assay and western blot analysis. Different patterns of TRAIL receptor expression were observed on the pancreatic cancer cell lines, including PANC1, HP62, ASPC1, TRM6 and BXPC3. Cell viability was assessed using a real-time monitoring system. Pancreatic cancer cell death was proportionally related to conditioned media from MSC nsTRAIL and MSC stTRAIL. The results suggest that MSCs exhibit intrinsic inhibition of pancreatic cancer cells and that this effect can be potentiated by TRAIL-transfection on death receptor-bearing cell types. © 2012 Nature America, Inc. All rights reserved.


Sun X.-Y.,303 Hospital of PLA | Nong J.,303 Hospital of PLA | Ke Q.,303 Hospital of PLA | Lu H.,University of British Columbia | And 3 more authors.
Anticancer Research | Year: 2011

Background: Mesenchymal stem cells (MSCs) have attracted great interest in cancer therapy since the discovery of their tumor tropism. This study was performed to investigate the effects of TNF-related apoptosis-inducing ligand (TRAIL)-engineered MSCs on hepatocellular carcinoma (HCC) cells (HepG2) under different culture conditions. Materials and Methods: MSCs engineered with non-secreting TRAIL (MSC TRAIL-GFP) (GFP, green fluorescence protein) and secreting TRAIL (MSC stTRAIL) were used for the direct co-cultures, and conditioned media (CM) from corresponding cultures were applied to HepG2 as indirect co-cultures. Immunoblotting, ELISA and FACS analysis were used to detect the expression of TRAIL and TRAIL receptors. Cell death was assessed using live/dead assay. Results: Death receptor (DR) 5 was identified on the HepG2 cells. The expression of TRAIL was confirmed in the cell lysates (MSC TRAIL-GFP >MSC stTRAIL) and the conditioned media (MSC stTRAIL >MSC TRAIL-GFP). Higher cell death was observed in high MSCIHepG2 ratio cocultures. HepG2 cell death was proportionally related to CM from MSC TRAIL-GFP and MSC stTRAIL. Conclusion: MSCs exhibit intrinsic inhibition of HepG2 which is potentiated by TRAIL-transfection.


Tang Z.,303 Hospital of PLA
Lin chuang er bi yan hou tou jing wai ke za zhi = Journal of clinical otorhinolaryngology, head, and neck surgery | Year: 2013

To investigate the clinical features and treatment options of ossifying fibroma of paranasal sinuses. A retrospective evaluation of twenty-three patients with ossifying fibroma of paranasal sinuses was presented. The choice of surgical operations on ossifying fibroma of paranasal sinuses was mainly decided by the location and area of ossifying fibroma. Radical operations were performed in twenty-one patients, ten of them through a lateral rhinotomy approach, eight through nasal endoscopic approach, four through Caldwell-Luc approach, one through coronal approach. Two patients were performed partial resection by nasal endoscopic surgery. Diagnoses of all cases were confirmed by pathology. All patients outcomes were successful, no serious complication from the surgical technique occurred. Twenty cases were followed-up for six months to nineteen years. Two patients recurred. Earlier diagnosis, CT scan, proper surgery, and radical resection are the keys to the treatment of ossifying fibroma of paranasal sinuses.


PubMed | 303 Hospital of PLA
Type: Journal Article | Journal: Anticancer research | Year: 2011

Mesenchymal stem cells (MSCs) have attracted great interest in cancer therapy since the discovery of their tumor tropism. This study was performed to investigate the effects of TNF-related apoptosis-inducing ligand (TRAIL)-engineered MSCs on hepatocellular carcinoma (HCC) cells (HepG2) under different culture conditions.MSCs engineered with non-secreting TRAIL (MSC(TRAIL-GFP)) (GFP, green fluorescence protein) and secreting TRAIL (MSC(stTRAIL)) were used for the direct co-cultures, and conditioned media (CM) from corresponding cultures were applied to HepG2 as indirect co-cultures. Immunoblotting, ELISA and FACS analysis were used to detect the expression of TRAIL and TRAIL receptors. Cell death was assessed using live/dead assay.Death receptor (DR) 5 was identified on the HepG2 cells. The expression of TRAIL was confirmed in the cell lysates (MSC(TRAIL-GFP) >MSC(stTRAIL)) and the conditioned media (MSC(stTRAIL) >MSC(TRAIL-GFP)). Higher cell death was observed in high MSC/HepG2 ratio co-cultures. HepG2 cell death was proportionally related to CM from MSC(TRAIL-GFP) and MSC(stTRAIL).MSCs exhibit intrinsic inhibition of HepG2 which is potentiated by TRAIL-transfection.

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