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Quanzhou, China

Wang Y.,Health Management Center | Jiang X.,Health Management Center | Dong S.,PLA 180 Hospital | Shen J.,PLA 180 Hospital | And 7 more authors.
Cancer Biomarkers | Year: 2016

BACKGROUND: The World Health Organization (WHO) has estimated that the number of cancer patients will increase by about 70% during the next 25 years world-wide. To deal with this problem, WHO has suggested a focus on prevention of tumor incidence and health screening for early detection of people with tumors. OBJECTIVE: To investigate the use of thymidine kinase 1 (TK1), CEA and AFP in serum to discover people with malignant tumors through health cancer screening. METHODS: Of a cohort in 486,085 people of a routine health screening at the Health Centre, Fujun 180 Hospital, Quanzhou city, China, 56,286 people were investigated according to the presence of cancer during 2009-2014. The concentration of CEA and AFP were determined by an electrochemiluminescence immunoassay from Roche Diagnostics e601GmbH and STK1 by a commercial kit based on an enhanced chemiluminescent dot blot assay. RESULTS: The cancer incident rate increased from 0.048/100,000 to 0.220/100,000. The most common types of tumors were those of the liver, cervix and lung. STK1 correlated to tumor growth rate, was more sensitive than CEA and AFP for discovering people with malignant tumors and more sensitive among people who had diagnosis of malignant tumor. STK1 was also a prognostic biomarker for death at 10-40 months follow-up, while CEA and AFP were not. A combination of these markers increased the sensitivity by about 30%. CONCLUSION: STK1 is a reliable biomarker for discovering people with malignant tumors in cancer screening. © 2016 - IOS Press and the authors.

Du Z.,PLA 180 Hospital | Dong S.,PLA 180 Hospital | Lin P.,PLA 180 Hospital | Chen S.,PLA 180 Hospital | And 6 more authors.
International Journal of Clinical and Experimental Medicine | Year: 2015

Biliary complications cause significant morbidity and mortality in liver transplantation. Warm ischemia can induce biliary duct injury. This study aimed to investigate the effects of warm ischemia on the peribiliary vascular plexus in rat liver transplantation. A total of 102 Sprague-Dawley rats were divided into three groups: sham-operation group, non-ischemic group, and ischemic group. Liver transplantation was performed in both the non-ischemic group and the ischemic group. The animals were sacrificed on day 1, 3, 7, and 14 to collect the blood and liver samples. Serum levels of bile duct obstruction, viz, alkaline phosphatase and gamma-glutamyl transpeptidase, as well as direct and indirect bilirubin were measured. Liver biopsy samples were examined with hematoxylin-eosin staining and transmission electron microscopy. The levels of enzymes and bilirubin were significantly higher in the ischemic group than the non-ischemic group and sham-operated animals (P<0.05), with return to normal levels in the ischemic group after two weeks. Morphological examination showed microthrombi and endothelial damage in the bile ducts and the peribiliary vascular plexus of the ischemic group. Warm ischemia/reperfusion injury can damage the endothelium of the peribiliary vascular plexus, which might compromise the bile duct microcirculation and lead to ischemic cholangiopathy after liver transplantation. © 2015 Int J Clin Exp Med. All Rights Reserved.

Wang H.-T.,PLA 180 Hospital | Liu A.-G.,PLA 180 Hospital | Luo D.-S.,Fujian Medical University | Zhou Z.-N.,PLA 180 Hospital | And 4 more authors.
Asian Pacific Journal of Tropical Medicine | Year: 2014

Objective: To investigate the expression of miR-218 and its clinical significance in osteosarcoma tissues and explore its effect on proliferation and apoptosis in osteosarcoma cells. Methods: miR-218 expression was detected in 76 samples of surgically resected osteosarcoma and matched normal tumor-adjacent tissues using quantitative reverse transcription polymerase chain reaction (qRT-PCR). MiR-218 was over-expressed by exogenous miR-218 plasmids in Saos-2 cells, and then BrdU cell proliferation assay and flow cytometry were used to determine cell proliferation and apoptosis. Results: The expression of miR-218 in osteosarcoma tissues was significantly lower than those in normal tumor-adjacent tissues (t=8.735, P<0.001). MiR-218 expression in tumor tissues was significantly correlated with tumor size (χ2=5.380, P=0.020), clinical stage (χ2=6.692, P=0.010) and distant metastasis (χ2=4.180, P=0.041). MiR-218 was obviously over-expressed by exogenous miR-218 plasmids (t=19.42, P<0.001), and miR-218 overexpression significantly reduced cell proliferation (t=9.045, P<0.001) and induced apoptosis (t=12.38, P<0.001) in Saos-2 cells. Conclusions: The low-expression of miR-218 is correlated with the poor clinicopathological features in osteosarcoma. Moreover, miR-218 overexpression reduces cancer cell proliferation and induces apoptosis in Saos-2 cells, suggesting that miR-218 may play a key role in the progression of human osteosarcoma. © 2014 Hainan Medical College.

Chen S.,PLA 180 Hospital | Lin P.,PLA 180 Hospital | Du Z.,PLA 180 Hospital | Lan F.,PLA 180 Hospital | And 6 more authors.
International Journal of Clinical and Experimental Medicine | Year: 2014

Transpulmonary thermodilution measurement is a convenient method for hemodynamic monitoring. However, the previously reported indicator temperature was not consistent. This study aimed to compare the accuracy of Pulse index Continuous Cardiac Output (PiCCO) monitoring using indicators of different temperatures. A total of 104 critically ill patients received PiCCO monitoring using indicators of either 0°C or 8°C. The PiCCO measurements, including general ejection fraction, global end-diastolic index, and cardiac index, were compared between the two temperatures, and were also correlated with that of transesophageal echocardiography (TEE). The two indicator temperatures differed significantly in hemodynamic measurements (P<0.01). PiCCO measurements with either indicator temperatures showed positive correlation with TEE results (P<0.05). The 0°C indicator had universally higher correlation coefficients than the 8°C indicator. So, PiCCO monitoring with the 0°C indicator might have better accuracy than the 8°C indicator. © 2014, E-Century Publishing Corporation. All rights reserved.

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