Gaziantep, Turkey
Gaziantep, Turkey

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Bay Turkoglu S.,University of Gaziantep | Coban N.,Pistachio Research Station | Ozkan A.,University of Gaziantep | Atli H.S.,Siirt State University | Can C.,University of Gaziantep
Turkish Journal of Agriculture and Forestry | Year: 2015

Seven almond varieties, Ferragnes, Nemaguard, AB3 (Amygdalus orientalis Mill.), GN22, GF677, Myrobalan (Prunus cerasifera), and Pissardi nigra, and F1 crossbreed almonds, Ferragnes × Pissardi nigra, Ferragnes × Myrobalan, Ferragnes × GN22, Nemaguard × GF677, Myrobalan × AB3 (Prunus dulcis Mill.), Myrobalan × Ferragnes, Pissardi nigra × AB3, and GF677 Myrobalan, were used to determine resistance against root-knot nematode (RKN) (Meloidogyne spp.) using DNA markers. Parental lines and 316 F1 plants were tested with SSR (plgms8, plgms9, and plgms19) and STS (STS-OPS14a, STS-834b) markers. It was found that plgms19 exhibited 76%, 77.01%, 73.13%, and 86.6% inheritance of resistance in the Ferragnes × Pissardi nigra, Ferragnes × Myrobalan, Ferragnes × GN22, and Nemaguard × GF677 F1 populations, respectively. No resistance was detected in the Nemaguard × GF677 population with the plgms8 marker. Since the plgms19 and STS-834b primers were found to be effective at high ratios in determining resistance to RKN in the Nemaguard × GF677 F1 population, the use of these markers could be included in breeding studies. It was also detected that the STS-OPS14a primer is appropriate to use for determining the sensitivity to RKN in almond rootstocks. © TÜBİTAK.


Can C.,University of Gaziantep | Konukoglu F.,Pistachio Research Station | Ozgun E.,University of Gaziantep | Kusek M.,Kahramanmaras Sutcu Imam University | And 4 more authors.
Acta Horticulturae | Year: 2014

Pistacia vera L. is an important crop species in the Southeastern region of Turkey. Alternaria blight disease caused by Alternaria spp. has become epidemic for the last couple of years. Disease symptoms mainly develop on young fruits and infections continue until harvest. Repeated infections decrease fruit quantity and quality that in turn reduce the yield. Pistachio fruits with Alternaria blight symptoms were collected during 2010-2011 growing seasons. Infected fruits were sampled from Adiyaman, Gaziantep, Sanliurfa, Mardin, Kahramanmaras and Siirt provinces where large pistachio plantations are located. Sampling and pathogen isolations were started in April just after pollination and fruit set, and continued until October, which is the harvest time of pistachio in the Southeastern Anatolia region. Isolates obtained from tissues infected with Alternaria blight were characterized morphologically and analyzed with DNA sequences, the nuclear internal transcribed spacer region, ITS and allergen regions, ALT, for molecular confirmation. Twenty-three Alternaria spp. isolates were obtained and subjected to pathogenicity analysis on 'Ohadi' pistachios. All the Alternaria spp. isolates caused blight symptoms on mature pistachio fruits. Four isolates were found to be highly pathogenic and there was difference among isolates in virulence levels. One highly virulent isolate was used to test reactions of 'Uzun', 'Ohadi' and 'Siirt' cultivars under field conditions, and 'Uzun' was found to be the most tolerant cultivar followed by 'Siirt' and 'Ohadi'.


Topcu H.,Cukurova University | Ikhsan A.S.,Cukurova University | Sutyemez M.,Kahramanmaras Sutcu Imam University | Coban N.,Pistachio Research Station | And 2 more authors.
Scientia Horticulturae | Year: 2015

Juglans regia L. is the only commercially grown species for nut consumption within the Juglans genus. Simple sequence repeats (SSRs) are the markers of choice, especially when constructing genetic linkage maps for plants. There are only a limited number of simple sequence repeat (SSR) markers in the literature for J. regia that can be used to construct a SSR based genetic linkage map. Therefore, genomic libraries enriched with CA, GA, AAC, and AAG repeats were constructed using genomic DNA from J. regia cv. 'Maraş-18' to develop SSR markers for walnut. A total of 624 clones were sequenced; 516 had repeats and 94 were duplicates. Two-hundred and seventy-six primer pairs were designed, and 246 generated PCR products and 185 polymorphic loci were obtained by characterizing 15 walnut cultivars. The number of alleles ranged from two to ten among the 185 polymorphic loci with an average of 4.3. The GA-enriched library was the best among the four libraries in terms of number of alleles, polymorphism, productivity, and information content. A higher number and frequency of SSRs were obtained from the dinucleotide-enriched libraries than from the trinucleotide libraries. The SSR markers developed in this study may help genetic studies on J. regia and related species, especially when constructing and integrating maps. © 2015 Elsevier B.V..


Acar I.,Harran University | Yilmaz A.,Pistachio Research Station | Arpaci S.,Pistachio Research Station
Acta Horticulturae | Year: 2014

A new almond breeding program was started in Turkey in 2009. The objectives of this program were aimed to obtain late flowering and self-compatible almond genotypes, crossing local and foreign cultivars. 'Lauranne', 'Guara' and 'Moncayo' were crossed with 'Gulcan 1', 'Gulcan 2' and 'Nurlu' cultivars. 1,686 seedlings with the Sf allele were obtained from crosses, and they were planted in the Pistachio Research Station, at 2 × 1 m distances, in winter 2011. Trunk diameter and leafing time of the seedlings were recorded one year after planting, to estimate the vigour and flowering time. Important differences were found between the families.


Sarpkaya K.,Pistachio Research Station | Yilmaz A.,Pistachio Research Station
Acta Horticulturae | Year: 2014

The usage of kaolin, called "particle film technology", has increased among most of agricultural crops in recent years. The effect of kaolin to control Septoria leaf spot (SLS) disease was investigated in this study. Three different doses of kaolin (1,5, 3 and 6%) were applied and 3% of dose was found to control SLS of pistachio with 39,06% effect. Single and multiple applications of kaolin were used and single application was more effective. This study also tested the combined effect of kaolin with propiconazole + difenoconazole (PD). The combined effect of kaolin with fungicide was found to control SLS more than kaolin alone. It is concluded that kaolin is not effective to control SLS disease on pistachio when it is applied without any combination with fungicide.

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