Pinnacle Biomedical Research Institute

Bhopal, India

Pinnacle Biomedical Research Institute

Bhopal, India
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Sachan R.,VNS Institute of Pharmacy | Khatri K.,VNS Institute of Pharmacy | Kasture S.B.,Pinnacle Biomedical Research Institute
International Journal of PharmTech Research | Year: 2010

Oral route is the easiest and most convenient route for drug administration. Oral drug delivery systems being the most cost-effective and leads the worldwide drug delivery market. The major problem in oral drug formulations is low and erratic bioavailability, which mainly results from poor aqueous solubility. This may lead to high inter- and intra subject variability, lack of dose proportionality and therapeutic failure. It is estimated that 40% of active substances are poorly water soluble (water insoluble in nature). For the improvement of bio-availability of drugs with such properties presents one of the greatest challenges in drug formulations. Various technological strategies are reported in the literature including solid dispersions, cyclodextrines complex formation, or micronization, and different technologies of drug delivery systems. Including these approaches self-emulsifying drug delivery system (SEDDS) has gained more attention for enhancement of oral bio-availability with reduction in dose. SEDDS are isotropic mixtures of oil, surfactants, solvents and co-solvents/surfactants. The principal characteristic of these systems is their ability to form fine oil-in-water (o/w) emulsions or micro-emulsions upon mild agitation following dilution by an aqueous phase. For lipophilic drugs, which have dissolution rate-limited absorption, SEDDS may be a promising strategy to improve the rate and extent of oral absorption. This review article explains how self-emulsifying drug delivery systems can increase the solubility and bioavailability of poorly soluble drug.


Waghulde H.,Mgvs Pharmacy College | Mohan M.,Mgvs Pharmacy College | Kasture S.,Pinnacle Biomedical Research Institute | Balaraman R.,M. S. University of Baroda
International Journal of PharmTech Research | Year: 2010

Acute subcutaneous administration of angiotensin-II (Ang II-150 μg/kg) causes a rise in blood pressure in normal Wistar rats. Administration of Punica granatum juice extract (PJ- 100 mg/kg and 300 mg/kg; p.o.) for 4 weeks in angiotensin-II treated rats significantly (P<0.05) reduced the mean arterial blood pressure and vascular reactivity changes to various catecholamines. PJ treatment significantly (P<0.05) decreased the serum levels of ACE and the levels of thiobarbituric acid reactive substances (TBARS); while enzyme activity of superoxide dismutase (SOD), catalase (CAT), glutathione reductase (GSH) in kidney tissue showed a significant elevation in PJ treated Ang II induced hypertensive rats. The cumulative concentration response curve (CCRC) of angiotensin-II was shifted towards right in rats treated with PJ using isolated strip of ascending colon. The results suggest that Punica granatum juice extract could prevent the development of high blood pressure induced by angiotensin-II probably by combating the oxidative stress and antagonizing the physiological actions of Ang II.


Ingale S.,Jawaharlal Nehru Technological University | Kasture S.,Pinnacle Biomedical Research Institute
International Journal of Green Pharmacy | Year: 2012

Passiflora incarnata also known as 'Passion flower' is used as an anxiolytic and sedative throughout the world from ancient time. The plant is used as an analgesic, antispasmodic, sedative- hypnotic and narcotic. It is also used in neuralgia, epilepsy, insomnia, ulcers, haemorrhoids and neurosis in many parts of the world. There was no report on analgesic activity of P. incarnata. Hence, the present study is designed to assess analgesic activity of leaves of P. incarnata using sodium chloride-induced eye wiping test and formalin test. In formalin test, n-butanol extract of leaves of P. incarnata (BEPI) in the doses of 150 and 300 mg/kg as well as BEPI-F1 showed significant reduction in duration of paw licking in neurogenic and inflammatory phase(P<0.001). Pretreatment with naloxone reversed the analgesia induced by BEPI, while atropine did not reverse the analgesia induced by BEPI significantly (P≤0.001). In eye wiping test, BEPI in the doses of 150 and 300 mg/kg, i.p. exerted significant reduction ( P≤0.001) in number of eye wipes compared to control group. Thus, the result concludes that BEPI and the fraction separated, BEPI-F1 has significant analgesic activity, which may be mediated through central mechanism by modulation of opioid receptors and nicotinic receptors.


Dwivedi J.,Banasthali University | Singh M.,Pinnacle Biomedical Research Institute | Sharma S.,Banasthali University
Journal of Herbs, Spices and Medicinal Plants | Year: 2017

The nephroprotective activity of hydro-alcoholic (HAEAM) and ethyl acetate (EAEAM) extracts of Aegle marmelos leaves were evaluated against nephrotoxicity induced by cisplatin (CP), a widely used chemotherapeutic agent in cancer therapy. Wistar rats were treated with CP (6 mg.kg– 1; i.p.). Treatment groups received the same dose of CP, along with HAEAM and EAEAM (200 and 400 mg.kg–1) orally for 5 d. Blood urea nitrogen (BUN), serum creatinine, and antioxidant enzymes were estimated in renal tissues. EAEAM exhibited minimum IC50 values 6.13 ± 1.05, 67.34 ± 1.7, 59.7 ± 3.9, and 49.17 ± 2.19 μg.mL– 1. EAEAM (400 mg.kg– 1) decreased the creatinine levels from 2.29 ± 0.387 to 0.96 ± 0.095 mg.dL– 1 and BUN from 92.06 ± 7.949 to 38.18 ± 5.686 mg.dL– 1 and restored the activities of renal antioxidant enzymes, decreased the lipid peroxidasde (LPO) levels from 158.70 ± 3.542 to 106.91 ± 5.876 nM.g– 1, and increased superoxide dismutase (SOD) levels from 12.59 ± 0.463 to 29.95 ± 5.222 U.g– 1, glutathione (GSH) from 0.24 ± 0.029 to 0.57 ± 0.048 μM.g– 1, and catalase (CAT) from 1.14 ± 0.067 to 3.27 ± 0.296 U.mg– 1). © 2017 Taylor & Francis Group, LLC


Kasture S.,Pinnacle Biomedical Research Institute | Kasture V.,Pinnacle Biomedical Research Institute
Oriental Pharmacy and Experimental Medicine | Year: 2013

Medicinal plants have been a rich source of medicines. Mucuna pruriens is extensively used in Ayurveda to treat kampavat (Parkinson's disease in modern medicine), a disease characterized by excess of Vata. Clinical and preclinical studies have substantiated claims on its efficacy and safety in PD and there are indications that it is more effective than the levodopa in reducing dyskinesias. Several constituents of Mucuna seeds such as genistein, gallic acid, unsaturated acids, nicotine, bufotenin, harmin alkaloids, lecithin, etc. have been isolated which possess neuroprotective activity and support the antiPD activity of levodopa. The review describes various constituents of Mucuna pruriens seeds in context to therapeutic utility in treating Parkinson's disease. Since the conventional treatment of PD using levodopa with other add-on drugs is very expensive and Mucuna pruriens seeds are easily available and economic, the use of standardized extract of Mucuna seeds may drastically reduce the cost of treatment and also reduce the progression of disease. The review emphasizes the importance of holistic approach of Ayurveda in using the Mucuna pruriens in treatment of PD. Further studies may provide an approach to understand the mechanisms involved in treating PD with lesser adverse effects. © 2013 Institute of Korean Medicine, Kyung Hee University.


Karchuli M.S.,Pinnacle Biomedical Research Institute
Asian Journal of Pharmaceutical and Clinical Research | Year: 2014

Present study was performed to evaluate the effect of Mentha arvensis on cisplatin-induced nephrotoxicity in Sprague-Dawley rats. The M. arvensis hydroalcoholic extract (MAHE) was administered orally at two dose levels (200 mg/kg and 400 mg/kg). The kidney function test (estimation of serum creatinine, total protein, blood urea nitrogen [BUN], and urea), oxidative stress study (estimation of superoxide dismutase [SOD] activity, glutathione content, and lipid peroxides [LPO]), and histological studies were also conducted. MAHE was found effective at both doses, although high dose (400 mg/kg) was found more effective, which was evidenced by decrease in serum creatinine, total protein, BUN, urea, and LPO and increased in SOD activity. Histopathological studies were also confirmed the nephroprotective action of MAHE. Present investigation revealed that M. arvensis showed nephroprotective effect on cisplatin nephrotoxicity in Sprague-Dawley rats which may be due to the presence of flavonoids and related compounds. © 2014 Asian Journal of Pharmaceutical and Clinical Research. All rights reserved.


Patil R.A.,MGVs Pharmacy College | Kasture S.B.,Pinnacle Biomedical Research Institute
Natural Product Research | Year: 2012

In this study, the neuroprotective potential and in vivo antioxidant status of extract of roots and rhizomes of Rubia cordifolia L (MERC) in reserpine-induced orofacial dyskinesia was studied. Reserpine (1 mg/kg, s.c.) on day 1, 3 and 5 was used to induce orofacial dyskinesia. At the end of treatment schedule, MERC significantly inhibited reserpine-induced vacuous chewing movements, tongue protrusions, orofacial bursts, catalepsy. MERC significantly increased locomotion and rearing in open field test. MERC exhibited significant elevation in the levels of superoxide dismutase, catalase, glutathione reductase and inhibition of lipid peroxidation in forebrain region, compared with the reserpine treated group. It significantly elevated dopamine levels in the forebrain region. GCMS revealed the presence of anthraquinones, having strong antioxidant activity. It is concluded that oxidative stress might play an important role in reserpine-induced abnormal oral movements and MERC significantly protected animals against reserpine-induced orofacial dyskinesia and has great potential in treatment of neuroleptic induced orofacial dyskinesia. © 2012 Taylor & Francis Group, LLC.


Patil R.A.,MGVs Pharmacy College | Hiray Y.A.,MGVs Pharmacy College | Kasture S.B.,Pinnacle Biomedical Research Institute
Indian Journal of Pharmacology | Year: 2012

Context: Reserpine-induced orofacial dyskinesia is an animal model of tardive dyskinesia which may be associated with neurodegeneration and free radical damage. Aim: The aim was to assess the neuroprotective potential and in vivo antioxidant status of alcoholic extract of roots and rhizomes of Nardostachys jatamansi (ANJ) and its triterpenes (TNJ) in reserpine-induced orofacial dyskinesia. Materials and Methods: In the present study, repeated treatment with reserpine (1.0 mg/kg) on each other day for a period of 5 days (days 1, 3, and 5) significantly induced vacuous chewing movements (VCMs) and tongue protrusions (TPs) in rats. The effect on reserpine-induced catalepsy was also studied. The effect of ANJ and TNJ on levels of superoxide dismutase (SOD), catalase (CAT), and glutathione reductase (GSH) and inhibition of lipid peroxidation (LPO) in the forebrain region was assessed. Statistical Analysis: All observations were expressed as mean ± SEM. Statistical analysis was performed by the one-way ANOVA followed by Dunnett′s test. P<0.05 was regarded as statistically significant. Results: At the end of the treatment schedule, ANJ and TNJ significantly inhibited reserpine-induced VCM, TP, and catalepsy, and significantly increased the locomotion and rearing in the open-field test. Treatment with ANJ and TNJ exhibited significant elevation in the levels of superoxide dismutase (SOD), catalase (CAT), and glutathione reductase (GSH) and inhibition of lipid peroxidation (LPO) in forebrain region compared to the reserpine treated group. Conclusions: The study concludes that ANJ and TNJ significantly protected animals against reserpine-induced orofacial dyskinesia as well as catalepsy suggesting its potential value in the treatment of neuroleptic-induced orofacial dyskinesia and Parkinson′s disease.


Mohan M.,Mgvs Pharmacy College | Kamble S.,Mgvs Pharmacy College | Gadhi P.,Computerised Laboratory | Kasture S.,Pinnacle Biomedical Research Institute
Food and Chemical Toxicology | Year: 2010

Nephrotoxicity is one of the important side effects of anthracycline antibiotics. The aim of the study was to determine the protective effect of Solanum torvum on doxorubicin-induced nephrotoxicity in rats using biochemical and histopathological approaches. Oxidative stress is the main factor in doxorubicin (DOX) induced nephrotoxicity. Wistar rats received either DOX (67.75 mg/kg, i.v, 2 days before sacrifice) or S. torvum (100 mg/kg and 300 mg/kg, p.o.) prior to DOX treatment or S. torvum (100 mg/kg and 300 mg/kg, p.o.) extract alone for 4 weeks. Nephrotoxicity was assessed by measuring the abnormal levels of serum creatinine and blood urea nitrogen (BUN). The anti-oxidant defence enzymes superoxide dismutase (SOD) and catalase (CAT) of kidney tissue were also measured at the end of the treatment schedule. Treatment with S. torvum (100 mg/kg and 300 mg/kg) significantly (p < 0.05) decreased the levels of creatinine and BUN and significantly (p < 0.05) increased the anti-oxidant defence enzyme levels of SOD and CAT. Histopathological changes showed that DOX caused significant structural damages to kidneys like tubular necrosis, renal lesions and glomerular congestion which was reversed with S. torvum. The results suggest that S. torvum has the potential in preventing the nephrotoxicity induced by doxorubicin. © 2009 Elsevier Ltd. All rights reserved.


Mohan M.,JKK Nataraja Dental College and Hospital | Waghulde H.,JKK Nataraja Dental College and Hospital | Kasture S.,Pinnacle Biomedical Research Institute
Phytotherapy Research | Year: 2010

Acute subcutaneous administration of Angiotensin II (Ang II) causes a rise in blood pressure in diabetic Wistar rats. Diabetes was induced using streptozotocin (70 mg/kg, i.v.). Chronic administration of pomegranate juice (PJ) extract (100 mg/kg and 300 mg/kg; p.o. for 4 weeks) obtained from Punica granatum (punicaceae) fruits reduced the mean arterial blood pressure and vascular reactivity changes to various catecholamines and also reversed the biochemical changes induced by diabetes and Ang II. PJ treatment also caused a significant decrease in levels of thiobarbituric acid reactive substances (TBARS) in kidney and pancreas while activities of enzymes superoxide dismutase (SOD), catalase (CAT), and glutathione reductase (GSH) showed significant elevation. The cumulative concentration response curve (CCRC) of Ang II was shifted towards right in rats treated with PJ using isolated strip of ascending colon. In histopathological examination, PJ treatment prevented the tubular degenerative changes induced by diabetes. The results suggest that the PJ extract could prevent the development of high blood pressure induced by Ang II in diabetic rats probably by combating the oxidative stress induced by diabetes and Ang II and by inhibiting ACE activity. In conclusion, PJ has antihypertensive action in Ang II diabetic model. Copyright © 2009 John Wiley & Sons, Ltd. 10.1002/ptr.3090.

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