Wang H.,Henan Agricultural University |
Yang H.,Henan Agricultural University |
Su F.,Henan Agricultural University |
Su F.,Kunming Dongchuan Tobacco Company |
And 4 more authors.
Acta Tabacaria Sinica | Year: 2014
Expression of key genes involved in sugar, nitrogen, starch and terpenoid metabolism pathways were tested by regulated deficit nitrogen nutrition to study effects of different nitrogen applications on carbon and nitrogen metabolism in flue-cured tobacco. Results indicated that in sugar metabolism pathway Inv and SuS genes' expression increased when tobacco leaf became more mature while not affected by early nitrogen level. Expression of SPS gene decreased with decline of nitrogen supply at early maturing stage. GBSSI gene in starch metabolism pathway was not affected by either maturity or early nitrogen supply. GS gene expressed at lower level in maturing stage and affected by early nitrogen supply while NR gene expression was not affected. HMGR gene expression in terpenoid metabolism pathway decreased when leaf became more mature and decreased with decline of early nitrogen supply. The assimilation of nitrogen and terpenoid metabolism were significantly affected when regulated deficit of nitrogen was applied at early growing stage. Sugar and starch metabolism were affected mostly at leaf maturing stage. ©, 2014, State Tobacco Monopoly Bureau and China Tobacco Society. All right reserved.