Biloxi, MS, United States
Biloxi, MS, United States
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Xie Y.,China Pharmaceutical University | Xie Y.,Shanghai Key Laboratory for Pharmaceutical Metabolite Research | Xie Y.,Shanghai JiaoTong University | He H.,Pine Biotech | And 4 more authors.
Journal of Pharmaceutical and Biomedical Analysis | Year: 2012

A sensitive and specific fluorescein isothiocyanate (FITC) label coupled with size-exclusion high-performance liquid chromatography-fluorescence detection (SE-HPLC-FLD) method was developed and validated for the estimation of the pharmacokinetic profiles of porcine fibrinogen after intraperitoneal injection of a porcine-derived fibrin glue (FG) to SD rats and beagle dogs with three single doses. Porcine fibrinogen, the major composition of the FG, was labeled with FITC. The FG containing FITC-labeled porcine fibrinogen was intraperitoneally administered to SD rats at three single dosages (100, 200, 400mg/kg of porcine fibrinogen), and the collected plasma was then detected by SE-HPLC-FLD method. The present technique was compared to the previously introduced isotope-labeled assay method for the pharmacokinetic studies in SD rats. The pharmacokinetic studies in SD rats showed that the correlation coefficient between the FITC-labeled assay and 125I-labeled assay methods was r 2=0.989. Thus, this FITC-labeled assay method performed well and demonstrated high concordance with the previous 125I-labeled assay method, suggesting that FITC-labeled assay could substitute the 125I-labeled assay as a method of choice for quantification in beagle dogs. Then the plasma levels of porcine fibrinogen in beagle dogs were studied by the FITC-labeled assay method with three single doses (15, 30, 60mg/kg of porcine fibrinogen).The method validation showed that the FITC label coupled with SE-HPLC-FLD method was suitable for the quantification of porcine fibrinogen in plasma samples with satisfactory linear (r 2>0.999), precision (<12%), accuracy (95.5-104.9%) and recovery (>88%). The results showed linear disposition of porcine fibrinogen at the examined dosage range in SD rats or beagle dogs. © 2011 Elsevier B.V.


PubMed | Fudan University, Pine Biotech and Shanghai JiaoTong University
Type: Journal Article | Journal: Journal of biomaterials applications | Year: 2015

A novel superhydrophilic hybrid scaffold was created by electrospinning a mixture of poly(l-lactic acid-co--caprolactone) and formulated fibrinogen. The hybrid scaffolds possess the combined benefits of each individual component, such as moderate mechanical strength and excellent biocompatibility. In vitro studies also revealed that endothelial cells seeded on the hybrid scaffolds achieved a relatively high level of cell attachment after three days of culture and a significant increase in the proliferation rate after seven days of culture, compared with pure fibrinogen or poly(l-lactic acid-co--caprolactone) scaffolds. A comparative study of hybrid and pure poly(l-lactic acid-co--caprolactone) patches was performed in an abdominal wall defect model in rats. In both groups, implants degraded by six months, but muscle reconstruction was only observed in the hybrid patch group.


Xie Y.,China Pharmaceutical University | Xie Y.,Shanghai Key Laboratory for Pharmaceutical Metabolite Research | Zhong G.,Fudan University | He H.,Pine Biotech | And 4 more authors.
Journal of Pharmaceutical and Biomedical Analysis | Year: 2011

The aim of the present study was to characterize the preclinical pharmacokinetics, tissue distribution and excretion profiles of porcine fibrinogen in rats after intraperitoneal injection of a porcine-derived fibrin glue. A sensitive and rapid isotope-labeled assay method was developed and validated for quantitative analysis in biological analysis. Porcine fibrinogen, the major composition of the fibrin glue, was radioiodinated with Na125I using the Iodo-Gen method. Following the purification and identification of 125I-porcine fibrinogen, the fibrin glue containing 125I-porcine fibrinogen was intraperitoneally administered to rats at three single dosages (100, 200, 400mg/kg of porcine fibrinogen). The results showed that the 125I-labeled assay method was suitable for the quantification of porcine fibrinogen in plasma samples, tissue samples and excreta samples with satisfactory linear (r2>0.998), precision (<13%), accuracy (95.9-104.2%) and recovery (>85%). After three single administrations, plasma concentration profiles showed a slow absorption phase with the mean tmax of 1.83-5.67h and a slow elimination proceeding with the terminal elimination half-life (T1/2) of 84.5-96.3h. Porcine fibrinogen was widely distributed to most of the tissues examined after a single intraperitoneal administration at 200mg/kg to rats. The radioactive porcine fibrinogen showed substantial disposition in liver, kidneys, stomach and intestine. Approximately 79.3% and 17.2% of administered radioactivity were recovered in urine and feces within 528h post-dosing, which indicated the major elimination route was urinary excretion. © 2010 Elsevier B.V.


Pine Biotech | Entity website

T-BIOINFO: a Bioinformatics Big Data Analysis Platform BioInfoPLATFORM is a flexible and user-friendly bioinformatics distributed computational system. It is meant to provide an intuitive and user -friendly interface for building pipelines (chains) of bioinformatics algorithms for the analysis of big data: NG genomics and transcriptomics sequencing, mass-spectroscopy proteomics/metabolomics, as well as phenotypic visual information and knowledge databases ...


Pine Biotech | Entity website


Pine Biotech | Entity website

Genome-wide fitness calculations enabled by CirSeq, combined with structural information, can provide high-definition, bias-free insights into structure-function relationships, potentially revealing novel functions for viral proteins and RNA structures, as well as nuanced insights into a viral genomes phenotypic space. Such analyses have the power to reveal protein residues or domains that directly correspond to viral functional plasticity and may significantly inform our structural and mechanistic understanding of hostpathogen interactions ...


Pine Biotech | Entity website

Modern advances in Personalized Medicine rely on technology that confirms a patients fundamental biology, DNA, RNA, or protein, which ultimately leads to confirming disease. For example, personalized medicine techniques such as genome sequencing can reveal mutations in the DNA code that lead to diseases ranging from cystic fibrosis to cancer


Pine Biotech | Entity website

Big data can be integrated with Agrotech to solve some of the biggest global issues such as climate change, genome sequencing, and food security. The use of biotechnology will be the core of agricultural sustainability, as its estimated the world population will reach nine billion by year 2025 ...


Pine Biotech | Entity website

Big Data Analysis opens up new opportunities for the world of Agrotech and Biotech. Our company delivers these solutions to help industries face the future ...

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