PIK Becej'

Bečej, Serbia

PIK Becej'

Bečej, Serbia
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Vidovic V.,Trg Dositeja Obradovica | Nemes Z.,PIK Becej | Popovic-Vranjes A.,Trg Dositeja Obradovica | Lukac D.,Trg Dositeja Obradovica | And 3 more authors.
Mljekarstvo | Year: 2013

In the current study, among 420 Holstein cows, the following ratios of κ-casein genotypes were found: 0.25 were AA, 0.52 were AB and 0.23 were BB. Frequencies of alleles A and B were 0.51 and 0.49, respectively. Cows with κ-casein AA and AB genotypes produced 322.72 and 464.73 kg more milk per year compared to cows of casein BB genotype (P<0.05), respectively. There were no significant differences in the amount of milk produced by cows of AA and AB casein genotypes. There were no statistically significant differences in overall yields (kilograms per year) of milk fat and milk protein between κ-casein genotypes of cows. In contrast, milk from genotype BB cows contained a significantly (P<0.05) higher percentage of milk fat compared to the milks of casein genotypes AA and AB. In spite of the different κ-casein genotypes having a significant influence on milk yield and fat content, heritability estimations showed that all three genotypes were of medium heritability, and heritability did not differ significantly between them. Variability values (of examined traits) were stable and large enough to ensure selection gains in future generations.


effects (recombination and heterosis) of cows with different proportions of Holstein-Friesian genes, obtained from the Serbian Fleckvieh (SF) and the Holstein-Friesian (HF) crossbreeding program in Vojvodina. Upgrading of local breeds with the Holstein-Friesian breed in Vojvodina started in 1971 and continued 2008. Six genotypes of cows (F1, R1, R2, R3, R4, R5) were obtained with increasing percentage of Holstein genes, in order to attain purebred Holstein cows. Of all obtained genotypes, cows of genotype R4 with a proportion of Holstein genes from 96.87 % had the highest lifetime milk production (20000 kg), followed by cows R3 with 19950 kg (93.75 % HF genes) and cows R5 with 19850 kg (98.44 % HF genes). Finally the process of upgrading resulted in pure Holsteins with 19780 kg of milk. The total lifetime production of milk fat did not show statistically significant difference (P>0.05) among the genotypes R1 - R5 which ranged from 675 to 690 kg. The pure Holstein obtained after sixth intermediate generations had the average lifetime milk fat production of 690 kg. With the increase in the proportion of Holstein-Friesian genes percentage of milk fat was decreased, so that the cows of genotypes R3, R4, R5 and pure Holsteins, had less than 3.5 % milk fat. In relation to the total milk yield, the highest realized heterosis effect was observed in the cows of F1 generation (hR F1=594 kg), while the lowest was observed in generation R2 (hR R2=72 kg), where negative effect of recombination was also found (rI R2=-77 kg). Positive values of the actual and relative of heterosis effect of the milk fat yield was observed in all genotypes, whereas the negative heterosis effect of the milk fat percentage was observed also in all genotypes, with the exception of R1 and R2 cows, in which the typical consequence of the positive recombination in the early crossed Holstein-generations was manifested. © 2014, Hrvatska Mljekarska Udruga. All rights reserved.


Vidovic V.,Trg Dositeja Obradovica | Lukac D.,Trg Dositeja Obradovica | Nemes Z.,PIK Becej | Trivunovic S.,Trg Dositeja Obradovica
Animal Science Papers and Reports | Year: 2014

In 765 Holstein-Friesian cows, the following ratios of β-lactoglobulin genotypes were found: 0.23 AA, 0.58 AB and 0.19 BB. The cows of AA genotype yielded more milk and milk fat than cows of genotype AB and BB. Cows with β-lactoglobulin AA and AB genotypes yielded respectively 224 and 190 kg more milk per lactation when compared to cows of BB genotype but the differences were not significant. There were significant (P<0.01) differences in the yield of milk protein produced by cows of the AB, BB and AA genotypes. Milk from cows of genotype AA and BB contained a slightly higher percentage of milk fat when compared to milk of cows with lactoglobulin genotype AB. Percentage of milk protein was higher in the milk of cows of genotype BB and AB. β-lactoglobulin genotype had a significant impact on the yield of milk protein (P<0.01), but had no influence on the other observed properties of milk.


Lukac D.,University of Novi Sad | Vidovic V.,University of Novi Sad | Zolt N.,PIK Becej | Zsolnai A.,Research Institute for Animal Breeding and Nutrition | And 2 more authors.
Mljekarstvo | Year: 2014

The aim of this study was to identify genotypes of transferrin, evaluate their frequency in population of Holstein-Friesian cows, and to study association of transferrin genotypes with productivity of cows. Blood samples were collected from 249 healthy adult Holstein cows, daughters of 18 sires, reared at farm in Vojvodina. The nine transferrin (Tf) genotypes were identified: three (transferrin AA, D1D1 and D2D2) of these were homozygous and the remaining six (transferrin AD1, AD2, AE, D1D2, D1E and D2E) heterozygous. The frequencies of genotypes AD2, D1D2, D2D2 and AD1 were 0.29, 0.20, 0.17, and 0.11, respectively, while the other genotypes had frequencies below 0.11. The frequency of alleles A, D1, D2 and E, which was derived from the frequency of genotypes, was 0.30 for allele A, 0.19 for allele D1, 0.45 for allele D2 and 0.06 for allele E. In the analyzed population, cows Tf genotype D2E, AD2, D2D2, and D1D2 had the highest average of milk and milk fat yield in the first three standard lactation, while cows genotype AA, AD1 and D1E had the least amount. Analysis of variance showed that Tf genotype had a non-significant effect in the case of milk fat. However, cows which showed the highest performance for milk fat yield in three standard lactations were phenovarients Tf D2E, AD2, D2D2 and D1D2 in the analyzed population. Based on these results, the general conclusion can be made that the cows with Tf genotype AD2 recorded best results in all observed traits, and that cows which were heterozygous for Tf gene had higher milk yield and milk fat than the cows homozygous for Tf gene.


Lukac D.,University of Novi Sad | Jovanovac S.,Josip Juraj Strossmayer University of Osijek | Nemes Z.,PIK Becej' | Vidovic V.,University of Novi Sad | And 3 more authors.
Mljekarstvo | Year: 2015

The aim of this study was to identify frequencies of alleles and genotypes, to evaluate their frequency in population of Holstein-Friesian cows, and to investigate association of κ-casein genotypes with longevity and lifetime production of cows. Blood samples were collected from 192 Holstein cows which have finished their production. Cows were reared at one farm in Vojvodina in Serbia. In the current study the following ratios of κ-casein genotypes were found: 0.50 were AA, 0.40 were AB and 0.10 were BB. Frequencies of alleles A and B were 0.70 and 0.30, respectively. Based on these results, it can be concluded that cows with heterozygous genotype AB had the longest life (2809 days) with the highest number of productive days (2062), while the cows which were homozygous recessive of genotype BB had the shortest life expectancy (2627 days) and the lowest number productive days (1878). The same trend was observed with regard to the production results of cows of these genotypes, where statistically significant differences have been observed (P<0.05) in the most traits between cows of heterozygous and homozygous genotypes, while between cows of dominant and recessive homozygous genotype statistically significant differences were not observed (P>0.05). The effect of dominant genes in observed traits was greater than the additive effect of genes. From the ratio of the additive and dominant gene effects, it can be seen that the average degree of dominance in observed traits was positive. © 2015, Hrvatska Mljekarska Udruga. All rights reserved.


Lukac D.,University of Novi Sad | Vidovic V.,University of Novi Sad | Nemes Z.,PIK Becej | Stupar M.,University of Novi Sad | Popovic-Vranjes A.,University of Novi Sad
Mljekarstvo | Year: 2013

The study included 765 Holstein cow for determining of P-lactoglobulin genotypes, 420 cows for determining K-casein genotypes and 252 cows for determining transferrin genotypes, daughters of 18 sires. Among 765 cows, 172 were of the P-LG AA genotype, 448 were of genotype AB, and 145 were of BB genotype. The frequencies of genotypes AA, AB and BB were 0.23, 0.58 and 0.19, respectively. The frequency of alleles A and B, which was derived from the frequency of genotypes, was 0.52 for allele A and 0.48 for allele B. Among 420 cows, 105 were of the K-casein AA genotype, 219 were of genotype AB, and 96 were of BB genotype. The frequencies of genotypes AA, AB and BB were 0.25, 0.52 and 0.23, respectively. The frequency of alleles A and B, which derived from the frequency of genotypes, was 0.51 for allele A and 0.49 for allele B. Among 249 cows, was identified 9 different genotypes of transferrin, 72 were of the Tf AD2 genotype, 50 were of genotype D1D2, 42 were of genotype D2D2, 28 were of genotype AD1, 20 were of genotype AA, 17 were of genotype D2E, 10 were of genotype AE and less than ten were of genotype D1D2 and D1E.The frequency of alleles A, D1, D2 and E, which was derived from the frequency of genotypes, was 0.30 for allele A, 0.19 for allele D1, 0.45 for allele D2 and 0.06 for allele E. In the studied population of Holstein Frie-sian dairy cattle in Serbia, a significant number of heterozygous individual and population variability were found. The large variability gives us the opportunity for further selection, favoring the genotype cows depending on the desired properties of milk(milk yield, content of milk fat and proteins) for further technological processing of milk. According to previous studies, it is obvious, that heterozygous cow tended to have a better production performance than the homozygous cows. This genetic information of polymorphic gene could be useful in marker assisted selection to improve production performance.


Zolt N.J.,Pik Becej | Vidovic V.S.,University of Novi Sad | Strbac L.J.,University of Novi Sad | Lukac D.R.,University of Novi Sad | Stupar M.R.,University of Novi Sad
CEFood 2012 - Proceedings of 6th Central European Congress on Food | Year: 2012

Capa casein genotyping was performed on 36 cows, bull dams, and based on the analysis of DNA from blood using PCR - REFL techniques. Served for the identification of allelesand genotypes capa casein. Using the identified allels (A and B) and genotypes (AA, AB and BB) capa casein evaluated their frequency in the examined population of cows. Frequency capa casein genotype were: 0,450 AA +0,494 AB + 0,056 BB = 1,00. Furthermore, the frequency of allels A and B which is derived from frequencies of genotypes was 0,66 for allele A and 0,34 for allele B. This reletionship expresses the preliminary information about presence of different genotypes capa casein in Holstein. The contribution capa casein and total casein genotype on milk coagulation rate, determined by LS method, model 1, the coagulation of milk was highly significant. Using model 2, the coagulation of milk, the amount of calcium present in milk had a high impact significantly and lactation order significant impact on the speed of milk coagulation. Physical and chemical composition of fresh milk casein genotypes of different hats, meets the current criteria for the quality of fresh milk. Acceptable rennet - coagulation properties of k-BB groups are reflected in a shortetime of coagulation of milk with rennet. In particular it is a significant relatioship that manifests increased content of casein and total protein in milk. The analysis showed that reflect the time making chesse milk k-BB group was almost three times faster than k-AA milk about 2 times faster than k-AB milk.

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