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Parnell, New Zealand

Caro Perez A.,Gennova Scientific S.L. Seville | Kumble S.,Pictor Ltd | Kumble K.D.,Pictor Ltd | Alonso Canizal M.C.,Gennova Scientific S.L. Seville | And 3 more authors.
Autoimmune Diseases | Year: 2014

The performance of immunoassays for the detection of autoantibodies is of critical importance in the diagnosis and assessment of patients with autoimmune connective tissue diseases (ACTD). Our objective was to compare the features of two multiplexed assays - INNO-LIA ANA and Gennova-PictArray ENA ELISA - for measurement of multiple autoantibodies and their utility as a clinical tool in ACTD diagnosis. The antigens included SS-A/Ro (60 and 52), SSB/La, Sm, Sm/RNP, CENP-B, Jo-1, and Scl-70. Stored sera from 85 ACTD patients and 80 controls consisting of patients with vasculitis, rheumatoid arthritis and infectious diseases, as well as healthy subjects were analyzed jointly with clinical and laboratory data. Agreement between the two methods varied between 58 and 99% (Cohen's kappa: 0.21-0.71) mostly for SSA and SSB. The frequency of specific autoantibodies measured using the two methods was more variable for SSA, SSB, and RNP/Sm. There were a higher number of ambiguous results when using INNO-LIA. The optimized cut-off values of the Gennova-PictArray resulted in over 99% specificities in samples obtained from the control group. Sensitivity patterns were more accurate in Gennova-PictArray than in INNO-LIA, as suggested in previously reported studies. A third method could be applied to determine which of the two methods is more accurate. © 2014 Alejandro Caro Pérez et al. Source

Pictor Ltd | Date: 2012-12-27

Imaging apparatus incorporating imaging software for reading biological assay results for scientific and research purposes. Medical imaging apparatus incorporating medical imaging software for reading biological assay results for diagnostic purposes.

Lopez-Muedano C.,University of Auckland | Lopez-Muedano C.,Pictor Ltd | Kirton R.S.,University of Auckland | Kumble K.D.,Pictor Ltd | Taberner A.J.,University of Auckland
Talanta | Year: 2012

The use of antibody-based diagnostic testing has increased significantly over the past decade, giving rise to a wide range of diagnostic devices. At one end of the cost-range are rapid inexpensive point-of-care tests based on immunochromatographic strips which provide a qualitative positive or negative test outcome. On the other hand, quantitative tests generally require the use of dedicated and expensive laboratory instruments. There remains a need for diagnostic instruments and tests that can provide quantitative assessment of disease markers at low cost. This paper describes the development of a novel low cost optical device for reading colorimetric and fluorescent immunodiagnostic test results. This portable instrument uses a webcam to capture test results from a specially designed 16-well slide containing a miniaturized array of test spots. Arrays are illuminated with either LEDs or lasers, while transmitted or emitted light is captured through a long-pass filter, allowing two different types of optical measurement to be performed within the same device. This device was used to read results from an array of antibodies conjugated with either an enzymatic or fluorescent tag resulting in a colored or fluorescent readout. © 2012 Elsevier B.V. Source

Pictor Ltd | Date: 2012-12-27

Medical diagnostic reagents and assays for testing of body fluids. Medical diagnostic instruments for the analysis of body fluids.

Kumble S.,Pictor Ltd | Choi L.,Pictor Ltd | Lopez-Muedano C.,Pictor Ltd | Kumble K.D.,Pictor Ltd
Journal of Clinical and Diagnostic Research | Year: 2012

Objective: This study was performed to demonstrate the use of an ELISA-based microarray technology which is termed as 'PictArrays', to identify autoantibody expression patterns in patients with symptoms of autoimmune connective tissue disease. Methods: Eight commonly tested antigens were simultaneously tested on specially designed 16-well slides for their autoantibody expression patterns. The assay specifcity, sensitivity and reproducibility for each of the antigens were measured. The results were analyzed by using specially developed algorithms to identify seropositive samples. Results: The multiplex assay could identify specifc antigen binding by autoimmune sera on the arrays. The PictArray sensitivity was similar to that which was obtained in established immunoassays, and the assay reproducibility was within limits which were acceptable for diagnostic uses. The software could correctly identify the positive antigen reactivity at concentrations as low as 2 units/ ml of the antibody. Conclusion: The data demonstrated the use of a multiplex platform to simultaneously measure multiple autoimmune antibodies. PictArrays offer signifcant advantages over other multiplex technologies, which include (i) the use of document scanners to read the test results (ii) ease of operation which requires no specialized technical training beyond that which is required for using the conventional ELISA kits (iii) reduction in errors through software-based data analysis, and (iv) inclusion of internal controls to monitor the assay performance of each sample. These features permit the use of PictArrays in resource-constrained laboratories using existing infrastructure without signifcant capital expenditure. Source

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