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San Raffaele Cimena, Italy

dos Santos L.C.,State University of Mato Grosso do Sul | Costa E.,UEMS | Leal P.A.M.,University of Campinas | Nardelli E.M.V.,PIBIC | de Souza G.S.A.,UEMS
Engenharia Agricola

The Brazilian Cerrado has numerous fruit species with economic potential for exploitation in commercial cultivation. In this context an experiment was developed with jatobá-do-cerrado seedlings at the State University of Mato Grosso do Sul / Aquidauana-MS, from October 29th, 2009 to February 25th, 2010, in a greenhouse covered with polyethylene film of 150μm, light diffuser, with thermal reflective screen of 50% of shading under film, front and side with monofilament screen of 50% of shading and the nursery with black monofilament screen of 50% of shading and closing of 45 degrees. In these environments the plants were grown in plastic pots of 5.0 L filled with 100% of soil, 100% of organic compound and levels of 10, 20, 30, 40, 50, 60, 70, 80 and 90 of organic compound added to the soil. The experiment was carried out in a completely randomized split-plot scheme with five replications. In both environments, plants have emerged more rapidly, higher growth and more biomass accumulation in the substrate without addition of organic compound. In this substrate the plants developed better in the greenhouse. The seedlings did not show positive response at the organic compost levels. The organic compound used increased the pH of the substrates, showed excess of certain nutrients and did not contribute to an effective seedling growth. Source

de Oliveira L.M.,Cx. Postal 354 | Bruno R.L.A.,PPGA DFCA CCA UFPB | da Silva K.R.G.,Federal University of Paraiba | Alves E.U.,PPGA DFCA CCA UFPB | And 2 more authors.
Revista Brasileira de Sementes

The catingueira (Caesalpinia pyramidalis Tul.) is a shrubby species of widespread occurrence in semiarid northeastern and endemic savanna biome. The irregularity and poor distribution of rainfall in the region to undertake seed production, to the point that most fruit has seeds badly formed or non-viable for germination. Thus, this study investigated the behavior of Caesalpinia pyramidalis Tul. seeds put into different packaging and storage ambient. The fruits were collected in São João do Carriri city - PB and taken to the Seed Analysis Laboratory, Universidade Federal da Paraíba where they were processed and the seeds stored in plastic bottles and glass, and kept under the following conditions: non controlled laboratory (27 ± 3°C and 64 ± 20% RH), freezer (-18°C) and dry chamber (19 ± 5°C and 74 ± 20% RH) for 225 days. Initially and each 45 days the seeds were submitted to determination of moisture content and the following evaluates: germination first and second count, germination speed index, length and seedling dry mass. It was observed that the viability and seed moisture content is maintained in all ambient and packaging studied in this paper, seed germination when analyzed by the first count test is best preserved when its are wrapped in plastic in a dry chamber and freezer ambient until the period of 135 days. Source

Bogo A.,Santa Catarina State University | Casa R.T.,Santa Catarina State University | Sangoi L.,Santa Catarina State University | Bianchet P.,UDESC | Saldanha A.,PIBIC
Summa Phytopathologica

The irrigation management system affects disease incidence and grain yield of rice. This work was carried out aiming to study the effect of soil drainage periods during tillering on the progress of leaf and panicle blast (Pyricularia grisea) and grain yield of paddy rice grown with pre-germinated seeds. Four periods of water drainage were tested: no drainage, soil drainage at tillering and reflooding at 7, 14 and 21 days after soil drainage. Two cultivars were evaluated for each irrigation system: Epagri 106 (short season) and Epagri 109 (late season). The cultivar's disease reaction was evaluated during the growing seasons of 2004/05 and 2005/06, in Pouso Redondo, SC, Brazil. Blast severity on leaves and panicles was determined in seven samples, performed from 40 to 90 and 100 to 140 days after rice sowing, respectively. Data were used to estimate the area under disease progress curve (AUDPC) of each cultivar. The water management system at tillering did not affect the area under disease progress curve (AUDPC) and grain yield. Smaller AUDPC values were presented by Epagri 106 for leaf and panicle bast. Grain yield ranged from 7.833 to 9.239 kg ha-1 (2004/2005) and from 3.984 to 9.040 kg ha-1 (2005/2006). High pluvial precipitation during the drainage periods prevented drought. This probably mitigated the effects of water management system on blast progress and rice grain yield. Source

do Vale Baracho N.C.,Pharmacology and Biochemistry | de Castro L.P.,PIBIC | da Cunha Borges N.,PIBIC | Laira P.B.,PIBIC
Acta Cirurgica Brasileira

Purpose: To evaluate the renal and hepatic function, through biochemical analysis after 14 days of creatine supplementation in physically inactive rats. Methods: Twenty four male, adult, Wistar rats were used which were kept in individual metabolic cages and were distributed into four groups, and received the following treatments by gavage:1) Control: distilled water; 2)Creatine 0.5g/Kg/day; 3) Creatine 1g/Kg/day; 4) Creatine 2g/Kg/day. Their urinary outputs as well as food and water intake were daily measured. At the end of the experiment, the animals were euthanized and serum samples were stored for biochemical analysis. Results: Creatine supplementation at the doses given produced no significant changes in plasma levels of aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, total protein, albumin, total cholesterol, HDL cholesterol, LDL cholesterol, VLDL cholesterol, triglycerides, glucose, creatinine, urea, and creatinine clearance, compared to control group (p> 0.05) Similarly, water and food intake, as well as urinary output, did not show significant changes among the four groups studied. Conclusion: At the doses used, oral creatine supplementation did not result in renal and/or hepatic toxicity. © 2015, Sociedade Brasileira para o Desenvolvimento de Pesquisa em Cirurgia. All rights reserved. Source

Rigamonti N.,PIBIC | Rigamonti N.,Vita-Salute San Raffaele University | Capuano G.,PIBIC | Ricupito A.,PIBIC | And 8 more authors.
Clinical Cancer Research

Purpose: Chronic inflammation, recruitment of myeloid-derived cells, and perturbation of the arginine metabolism have been all proposed as mechanisms favoring prostate carcinogenesis and tumor immunoescape. Objective of this study was to evaluate whether accumulation of CD11b+Gr1+ cells, also defined myeloid-derived suppressor cells, occur in mice affected by transplantable or spontaneous prostate cancer (PC). We also investigated whether N(G) nitro-L-arginine methyl ester (L-NAME) and sildenafil, both modulators of the arginine metabolism, restrain tumor growth and restore tumor-specific immunity. Experimental Design: Wild-type C57BL/6 mice bearing TRAMP-C1 PC and transgenic adenocarcinoma of the mouse prostate (TRAMP) mice were treated with vehicle, L-NAME or sildenafil, and evaluated for CD11b+ cells accumulation in the blood, several organs, and the tumor mass and for disease progression. Results: CD11b+Gr1high, CD11b +Gr1int, and CD11b+Gr1- cells differently accumulated in different organs and especially in the tumor of the two mouse models. L-NAME and sildenafil impaired the immunosuppressive function of CD11b+ cells in both models and restrained TRAMP-C1 growth, but they neither break tumor-specific immune tolerance nor limit tumor progression in TRAMP mice. Conclusions: Collectively, our results emphasize substantial differences in tumor-induced alteration of myelopoiesis and sensitivity to modulators of the arginine metabolism between a transplantable and a spontaneous model of PC. They also suggest that perturbation of the arginine metabolism is dispensable for PC progression and the associated T-cell tolerance. ©2011 AACR. Source

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