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Patent
Dh Technologies Development Pte. Ltd. and Phytronix Technologies Inc. | Date: 2012-10-26

Methods and systems for quantifying analytes in a biological sample are provided comprising preparing a biological sample for mass spectrometric analysis, utilizing an ionization source to ionize at least a portion of the prepared biological sample to generate an ionized analyte flow, introducing the ionized analyte flow into a differential mobility spectrometer set at a compensation voltage selected to extract ionized analyte molecules from the ionized analyte flow, introducing an output analyte flow of the differential mobility spectrometer into a mass spectrometer to detect and quantify analyte ions in the output analyte flow.


Segura P.A.,University of Montreal | Tremblay P.,Phytronix Technologies Inc. | Picard P.,Phytronix Technologies Inc. | Gagnon C.,Environment Canada | Sauve S.,University of Montreal
Journal of Agricultural and Food Chemistry | Year: 2010

Sulfonamides are antibiotic compounds widely used in the dairy industry. Their presence in diary milk poses a risk to public health and may also contribute to the spread of antibiotic resistance in bacteria. Sulfonamide residues in dairy milk were quantified by tandem mass spectrometry (MS/MS) using a novel ionization source based on laser diode thermal desorption-negative mode atmospheric pressure chemical ionization (LDTD-APCI(-)). Seven sulfonamides spiked in milk were extracted with acetonitrile, which yielded high recoveries (77.5-101.5%). Calibration curves in the matrix showed good linearity (0.9977 ≥R 2≥ 0.9658) over the dynamic range (1.6-500 μg L -1), and limits of quantitation were between 2 and 14 [μg L -1, lower than or of the same magnitude as maximum residue criteria set by several regulatory agencies (10-100 ng L -1). In addition, the run time using the LDTD-MS/MS system was 30 s per sample, as compared to actual methods running from 7 to 84 min for the same sulfonamide residue compounds, which gave the method the high screening throughput capacity necessary for monitoring milk production. © 2010 American Chemical Society. Source


Blachon G.,Phytronix Technologies Inc. | Picard P.,Phytronix Technologies Inc. | Tremblay P.,Phytronix Technologies Inc. | Demers S.,Laval University | And 3 more authors.
Journal of AOAC International | Year: 2013

A high-throughput, rapid, and reliable method based on laser diode thermal desorption (LDTD) and atmospheric pressure chemical ionization coupled to tandem MS (APCI-MS/MS) was used to identify and quantify chloramphenicol (CAP) residues in honey. Sample pretreatment consisted of a liquid-liquid extraction of diluted honey in water with ethyl acetate containing stearic acid. After extraction, a 2 μL aliquot of the organic phase was deposited into a 96-well plate prior to detection by LDTD-APCI-MS/MS. The total analysis time was less than 6 s compared to several minutes for traditional chromatographic methods used for CAP detection, since no chromatographic separation was necessary. The extraction and analysis were made with honey samples of different color grading ranging from extra white to dark amber. The method showed good linearity (R 2 = 0.99995) within a concentration range of 0.1 to 500 ng/g for CAP with D5-CAP as an internal standard. The RSD varied between 8 and 24% over the calibration range (n = 4). The method LOD for CAP in honey was 0.19 ng/g. This work demonstrates that LDTD-APCI-MS/MS could be used for fast and effective quantification of CAP in honey samples. Source


Badjagbo K.,University of Montreal | Badjagbo K.,Phytronix Technologies Inc. | Loranger S.,QSAR Risk Assessment Service | Moore S.,Center dExpertise en Analyse Environnementale du | And 2 more authors.
Human and Ecological Risk Assessment | Year: 2010

We measured the concentrations of benzene, toluene, ethylbenzene, and xylenes (BTEX) in the ambient air of automobile repair garages in Montreal, Canada, using the direct atmospheric pressure chemical ionization-tandem mass spectrometry (APCI-MS/MS) method. Among all the air samples analyzed, toluene was the most abundant BTEX-species (127-1101 μg/m3) followed by xylenes (50-323 μg/m3), ethylbenzene (11-65 μg/m3), and benzene (9.2-23 μg/m3). BTEX levels where ventilation was controlled simultaneously by both mechanical and natural systems were significantly less than levels at garages where only natural ventilation was used. Results suggest that multiple sources contribute to the occupational exposure of automobile mechanics and painters to the BTEX. Owing to the toxic effects of these chemicals, both chronic non-cancer hazard and integrated lifetime cancer risk due to the exposure of this occupational group were assessed. The levels of the BTEX measured at all the garages were less than the established limits for occupational exposure; still, benzene levels pose a potential cancer risk for the workers. At the prevailing levels of BTEX, they may not cause any chronic non-cancer problems for the workers. © Taylor & Francis Group, LLC. Source


Heudi O.,Novartis | Barteau S.,Novartis | Picard P.,Phytronix Technologies Inc. | Tremblay P.,Phytronix Technologies Inc. | And 2 more authors.
Journal of Pharmaceutical and Biomedical Analysis | Year: 2011

An ultra-fast, reliable and sensitive analytical method enabling high-throughput quantitative analysis of pharmaceutical compounds in human plasma is described. The quantitative work was performed on one of our compound currently under clinical trial by employing a deuterated internal standard (IS). Plasma samples were treated on solid phase micro-extraction (SPME) plates prior their analysis by laser diode thermal desorption and atmospheric pressure chemical ionization coupled to tandem mass spectrometry (LDTD/APCI-MS/MS) in positive mode. The sample analysis run time was 10s as compared to the 7min obtained for the validated LC-MS/MS method. The limit of quantification (LOQ) of the method was estimated at 1ng/mL. The calibration graphs were linear with a regression coefficient R2>0.997. The data of the partial validation show that LDTD/APCI-MS/MS assay was highly reproducible and selective. In addition, the deviations for intra and inter assay accuracy and precision data were within 15% at all quality control levels. The LDTD/APCI-MS/MS method was successfully applied to the analysis of clinical samples and the data obtained were consistent with those found with a validated LC-MS/MS assay. This work demonstrates that LDTD/APCI-MS/MS could be used for the ultra-fast and reliable quantitative analysis of pharmaceutical compounds in human plasma without using the separation step commonly associated with the LC-MS/MS assay. © 2010 Elsevier B.V. Source

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