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PubMed | Phytopharm plc
Type: | Journal: Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association | Year: 2012

Hoodia gordonii extract contains steroid glycosides, fatty acids, plant sterols and polar organic material. Certain steroid glycosides show appetite suppressant activities following oral ingestion. This study describes the validation of a bioanalytical method for the quantification of one of the steroid glycosides, H.g.-12 ( 10% (w/w) of the extract), in mouse, rat, rabbit and human plasma. The method utilises a liquid-liquid extraction with methyl-tert-butyl ether followed by chromatographic separation on a 2.1 50 mm C(18) Genesis high performance liquid chromatography (HPLC) column and detection on a triple quadrupole mass spectrometer. Detection of H.g.-12 and its stable isotope internal standards is performed using positive TurboIonspray ionisation in multiple reaction monitoring mode. The validation procedure demonstrated assay sensitivity, linearity, accuracy, precision and selectivity over the calibration range of 0.5-150 ng/mL in human plasma (500 L sample volume), 1.0-100 ng/mL in rat and rabbit plasma (150 L sample volume) and 1.0-250 ng/mL in mouse plasma (150 L sample volume) with good recoveries ( 77%). H.g.-12 was stable in plasma for 6 months at -20C, for up to 4h at ambient temperature (ca22C) and after 3 freeze-thaw cycles. Plasma extracts were stable for up to 24h at ambient temperature.


Trademark
Phytopharm Plc | Date: 2013-01-01

Chemicals used in industry and science; chemicals for use in the manufacture of medical and veterinary products; chemicals being artificial plant extracts for use in industry and science. Pharmaceutical and veterinary preparations for treatment in the field of neurological and neurodegenerative diseases, ophthalmology and central nervous system disorders; plant extracts for medical and veterinary use; nutritional supplements for medical and veterinary use; food supplements for medical and veterinary use.


PubMed | Phytopharm plc
Type: | Journal: Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association | Year: 2012

Hoodia gordonii (Masson) Sweet ex Decne., is a succulent shrub, indigenous to the arid regions of southern Africa. Indigenous people have historically utilised certain species of Hoodia, including H. gordonii, as a source of food and water. Studies by the Council for Scientific and Industrial Research (CSIR, South Africa) identified that extracts of H. gordonii had appetite suppressant activity associated with specific steroid glycosides. A programme to develop weight management products based around this discovery was implemented in 1998. An agronomy programme was established which demonstrated that it was possible to cultivate this novel crop on a commercial scale (in excess of 70 ha). In parallel, a food grade manufacturing process was developed consisting of four main steps: harvesting of H. gordonii plant stems, comminution, drying under controlled conditions and extraction using food grade solvents. Appropriate Quality Control (QC) procedures were developed. The extraction process is capable of delivering a consistent composition despite natural variations in the composition of the dried H. gordonii. Specifications were developed for the resulting extract. The intended use of the standardised H. gordonii extract was as a functional food ingredient for weight management products. Other development studies on characterisation, toxicology and pharmacology are reported separately.


Trademark
Phytopharm Plc | Date: 2011-10-13

Chemicals used in industry and science; chemicals for use in the manufacture of medical and veterinary products; chemicals being artificial plant extracts for use in industry and science. Pharmaceutical and veterinary preparations for treating neurodegenerative diseases, ophthalmological disorders and diseases, central nervous system disorders and diseases, and inflammatory disease; pharmaceutical preparations for the treatment of infectious diseases, and diseases and disorders in the field of, oncology, hepatology, nephrology, cardiovascular, vascular, respiratory, serology, immunology, hematology, metabolism, neurology and neurodegeneration, neuropathies, pain, gastrointestinal, dermatology, muscoskeletal, central nervous system, endocrinology, hormonal, psychiatric, dementia, urology, obstetrics and gynaecology, ophthalmology, inflammation, odontology, dentistry, cognition, genetic disorders, cerebrovascular, eating disorders, hearing, hypothalamic and pituitary diseases, and sleep disorders. Scientific and technological services and research and design relating thereto, namely, medical research, chemistry research, biochemistry research, pharmaceutical research; research and development of pharmaceuticals and veterinary preparations and products; biological, biochemical and chemistry research services; drug discovery services; medical and scientific research, namely, conducting clinical trials for others; preclinical services, namely, scientific research services in the nature of characterization, optimization, profiling of efficacy, toxicity and therapeutic effect of drugs and scientific research analysis, testing and monitoring of biological and chemical material in the area of identification and characterization of medical and veterinary conditions, illnesses and diseases; scientific research and consultancy services in the fields of preclinical and clinical trials for pharmaceuticals and veterinary preparations and products; medical and veterinary research services. Medical services; veterinary services; medical analysis for the diagnosis and treatment of persons; veterinary analysis for the diagnosis and treatment of animals and birds; medical and veterinary consulting services in the field of pharmaceutical and veterinary products; medical and veterinary consulting services in the field of plant extracts for pharmaceutical and veterinary use; clinical diagnostic services, namely, investigation in the nature of diagnosis, analysis, testing and monitoring of patients for the purpose of identification and characterization of patients before, during and after therapy or treatment.


Trademark
Phytopharm Plc | Date: 2013-01-01

Chemicals used in industry and science; chemicals for use in the manufacture of medical and veterinary products; chemicals being artificial plant extracts for use in industry and science. Pharmaceutical and veterinary preparations for treatment in the field of neurological and neurodegenerative diseases, ophthalmology and central nervous system disorders; plant extracts for medical and veterinary use; nutritional supplements for medical and veterinary use; food supplements for medical and veterinary use.

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