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Utrilla M.P.,University of Granada | Peinado M.J.,Physiology and Biochemistry of Animal Nutrition EEZ | Ruiz R.,Physiology and Biochemistry of Animal Nutrition EEZ | Rodriguez-Nogales A.,University of Granada | And 5 more authors.
Molecular Nutrition and Food Research | Year: 2015

Scope: This study investigates the preventive effects of two pea (Pisum sativum) seed albumin extracts, either in the presence (pea seed extract [PSE]) or absence (albumin fraction from PSE [AF-PSE]) of soluble polysaccharides, in the dextran sodium sulfate (DSS) induced colitis in mice. Methods and results: Male C57BL/6J mice were assigned to five groups: one noncolitic and four colitic. Colitis was induced by incorporating DSS (3.5%) in the drinking water for 4 days, after which DSS was removed. Treated groups received orally PSE (15 g/kg{dot operator}day), or AF-PSE (1.5 g/kg{dot operator}day), or pure soy Bowman-Birk inhibitor (BBI; 50 mg/kg{dot operator}day), starting 2 wk before colitis induction, and maintained for 9 days after. All treated groups showed intestinal anti-inflammatory effect, evidenced by reduced microscopic histological damage in comparison with untreated colitic mice. The treatments ameliorated the colonic mRNA expression of different proinflammatory markers: cytokines, inducible enzymes, metalloproteinases, adhesion molecules, and toll-like receptors, as well as proteins involved in maintaining the epithelial barrier function. Furthermore, the administration of PSE, AF-PSE, or soy BBI restored bacterial counts, partially or totally, to values in healthy mice. Conclusion: PSE and AF-PSE ameliorated DSS-induced damage to mice, their effects being due, at least partially, to the presence of active BBI. © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. Source


Rubio L.A.,Physiology and Biochemistry of Animal Nutrition EEZ | Perez A.,Physiology and Biochemistry of Animal Nutrition EEZ | Ruiz R.,Physiology and Biochemistry of Animal Nutrition EEZ | Guzman M.A.,Physiology and Biochemistry of Animal Nutrition EEZ | And 2 more authors.
Journal of the Science of Food and Agriculture | Year: 2014

BACKGROUND: Legume seed proteins have to be chemically characterized in order to properly link their nutritional effects with their chemical structure. RESULTS: Vicilin and albumin fractions devoid of cross-contamination, as assessed by mass peptide fingerprinting analysis, were obtained from defatted pea (Pisum sativum cv. Bilbo) meal. The extracted protein fractions contained 56.7-67.7 g non-starch polysaccharides kg-1. The vicilin fraction was higher than legumins in arginine, isoleucine, leucine, phenylalanine and lysine. The most abundant amino acids in the albumin fraction were aspartic acid, glutamic acid, lysine and arginine, and the amounts of methionine were more than double than those in legumins and vicilins. The pea albumin fraction showed a clear enrichment of protease inhibitory activity when compared with the seed meal. In vitro digestibility values for pea proteins were 0.63± 0.04, 0.88± 0.04 and 0.41± 0.23 for legumins, vicilins and albumins respectively. CONCLUSION: Vicilin and albumin fractions devoid of cross-contamination with other proteins were obtained from pea seed meal. The vicilin fraction also contained low amounts of soluble non-starch polysaccharides and was enriched in isoleucine, leucine, phenylalanine and lysine. In vitro digestibility values for pea proteins were similar or even numerically higher than those for control proteins. © 2013 Society of Chemical Industry. Source

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