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Neri G.,Messina University | Micale N.,Messina University | Scala A.,Messina University | Fazio E.,Physical science and Earth science MIFT | And 9 more authors.
FlatChem | Year: 2017

Graphene and its multifunctional derivatives may offer passive targeting toward tumor sites and could be designed as drug delivery systems. Surface modifications of graphene materials convey specific biological activity to themselves and improve their biocompatibility. Herein we report the conjugation of Silibinin, a flavonoid employed mainly as hepatoprotective and anticancer agent, to a graphene modified nanoplatform. The cytotoxicity of the new platform has been evaluated on human mesenchymal stem cells and the anticancer effects have been studied on a human osteosarcoma cell line. Our graphene nanoplatform did not show any cytotoxicity even at high concentration (1000 μg/ml) and Silibinin grafted onto graphene maintained its antiproliferative activity. © 2016 Elsevier B.V.


Scibilia S.,Physical science and Earth science MIFT | Lentini G.,Messina University | Fazio E.,Physical science and Earth science MIFT | Franco D.,Messina University | And 3 more authors.
Sensing and Bio-Sensing Research | Year: 2016

Biohybrid nanostructured materials, composed of both inorganic nanoparticles and biomolecules, offer prospects for many new applications in extremely diverse fields such as chemistry, physics, engineering, medicine and nanobiotechnology. In the recent years, Phage display technique has been extensively used to generate phage clones displaying surface peptides with functionality towards organic materials. Screening and selection of phage displayed material binding peptides has attracted great interest because of their use for development of hybrid materials with multiple functionalities. Here, we present a self-assembly approach for the construction of hybrid nanostructured networks consisting of M13 P9b phage clone, specific for Pseudomonas aeruginosa, selected by Phage display technology, directly assembled with silver nanoparticles (AgNPs), previously prepared by pulsed laser ablation. These networks are characterized by UV-vis optical spectroscopy, scanning/transmission electron microscopies and Raman spectroscopy. We investigated the influence of different ions and medium pH on self-assembly by evaluating different phage suspension buffers. The assembly of these networks is controlled by electrostatic interactions between the phage pVIII major capsid proteins and the AgNPs. The formation of the AgNPs-phage networks was obtained only in two types of tested buffers at a pH value near the isoelectric point of each pVIII proteins displayed on the surface of the clone. This systematic study allowed to optimize the synthesis procedure to assembly AgNPs and bacteriophage. Such networks find application in the biomedical field of advanced biosensing and targeted gene and drug delivery. © 2016.

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