New Philadelphia, PA, United States
New Philadelphia, PA, United States

Philadelphia University , founded in 1884, is a private university in the East Falls neighborhood of Philadelphia, Pennsylvania, United States. The student body has about 3,500 students. Academic programs are divided among the College of Architecture and the Built Environment; the Kanbar College of Design, Engineering and Commerce; and the College of Science, Health and the Liberal Arts; the School of Continuing and Professional Studies; coursed are also offered via PhilaU Online. Philadelphia University awards bachelor's degrees, master's degrees and doctoral degrees. Wikipedia.


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The primary aims of this work were to: 1) establish a calibrator surrogate matrix for quantification of amyloid-β (Aβ)42 in human cerebrospinal fluid (CSF) and preparation of quality control samples for LC-MS-MS methodology, 2) validate analytical performance of the assay, and 3) evaluate its diagnostic utility and compare it with the AlzBio3 immunoassay. The analytical methodology was based on a 2D-UPLC-MS-MS platform. Sample pretreatment used 5 M guanidine hydrochloride and extraction on μElution SPE columns as previously described. A column cleaning procedure involved gradual removal of aqueous solvents by acetonitrile assured consistent long-term chromatography performance. Receiver-operator characteristic (ROC) curve and correlation analyses evaluated the diagnostic utility of UPLC-MS-MS compared to AlzBio3 immunoassay for detection of Alzheimer's disease (AD). The surrogate matrix, artificial CSF containing 4 mg/mL of BSA, provides linear and reproducible calibration comparable to human pooled CSF as calibration matrix. Appropriate cleaning of the trapping and analytical columns provided every-day, trouble-free runs. Analyses of CSF Aβ42 showed that UPLC-MS-MS distinguished neuropathologically-diagnosed AD subjects from healthy controls with at least equivalent diagnostic utility to AlzBio3. Comparison of ROC curves for these two assays showed no statistically significant difference (p = 0.2229). Linear regression analysis of Aβ42 concentrations measured by this mass spectrometry-based method compared to the AlzBio3 immunoassay showed significantly higher but highly correlated results. In conclusion, the newly established surrogate matrix for 2D-UPLC-MS-MS measurement of Aβ42 provides selective, reproducible, and accurate results. The documented analytical performance and diagnostic performance for AD versus controls supports consideration as a candidate reference method.


Matkar S.,Philadelphia University | Thiel A.,Philadelphia University | Hua X.,Philadelphia University
Trends in Biochemical Sciences | Year: 2013

The protein menin is encoded by the MEN1 gene, which is mutated in patients with multiple endocrine neoplasia type 1 (MEN1) syndrome. Although menin acts as a tumor suppressor in endocrine organs, it is required for leukemic transformation in mouse models. Menin possesses these dichotomous functions probably because it can both positively and negatively regulate gene expression, as well as interact with a multitude of proteins with diverse functions. Here, we review the recent progress in understanding the molecular mechanisms by which menin functions. The crystal structures of menin with different binding partners reveal that menin is a key scaffold protein that functionally crosstalks with various partners to regulate gene transcription and interplay with multiple signaling pathways. © 2013 Elsevier Ltd.


Insomnia is a common health complaint world-wide. Insomnia is a risk factor in the development of other psychological and physiological disorders. Therefore understanding the mechanisms which predispose an individual to developing insomnia has great transdiagnostic value. However, whilst it is largely accepted that a vulnerable phenotype exists there is a lack of research which aims to systematically assess the make-up of this phenotype. This review outlines the research to-date, considering familial aggregation and the genetics and psychology of stress-reactivity. A model will be presented in which negative affect (neuroticism) and genetics (5HTTLPR) are argued to lead to disrupted sleep via an increase in stress-reactivity, and further that the interaction of these variables leads to an increase in learned negative associations, which further increase the likelihood of poor sleep and the development of insomnia. Copyright © 2013 Elsevier Ltd. All rights reserved.


Soufi A.,Philadelphia University
Current Opinion in Genetics and Development | Year: 2014

During development, the genome adopts specific chromatin states to establish and maintain functionally distinct cell types in a well-controlled environment. A select group of transcription factors have the ability to drive the transition of the genome from a pluripotent to a more specialized chromatin state. The same set of factors can be used as reprogramming factors to reset the already established chromatin state back to pluripotency or directly to an alternative cell type. However, under the suboptimal reprogramming conditions, these factors fall short in guiding the majority of cells to their new fate. In this review, we visit the recent findings addressing the manipulation of chromatin structure to enhance the performance of transcription factors in reprogramming. The main emphasis is on the mechanisms underlying the conversion of somatic cells to pluripotency using OSKM. This review is intended to highlight the windows of opportunities for developing mechanistically based approaches to replace the phenotypically guided methods currently employed in reprogramming, in an attempt to move the field of cell conversion towards using next generation technologies. © 2014 Elsevier Ltd.


Walsh J.,Philadelphia University
Environment and Planning D: Society and Space | Year: 2013

This paper analyzes the politics of mapping along the US-Mexico border to explore how humanitarian activists wield geospatial technologies in challenging border securitization. In addition to 'bounding' societies within delimited territorial zones, mapping technologies have recently been elevated to encompass observing, locating, and tracking mobile bodies-outcomes that, in the context of immigration control, have heightened the risk of clandestine border crossings and produced a sharp rise in migrant fatalities. As geospatial tools have been integrated into official gatekeeping strategies, activists have deployed them to protect migrants, promote mobility rights, and disrupt borders and the spatial, legal, and technical orders underpinning their materialization and enforcement. This case study considers the political implications of these interventions, and highlights how technologies traditionally aligned with state security and surveillance have been reappropriated in the service of transnational solidarity, recognition, and hospitality.


Patent
Philadelphia University | Date: 2013-02-15

A method for removing airborne bacteria and airborne mycetes from indoor air comprises imparting microbiocidal and microbiostatic properties to fabric by treating the fabric with one or more naturally occurring biocides, positioning the treated fabric in a sheet-like orientation, and blowing indoor air through the fabric.


Patent
Philadelphia University | Date: 2014-03-17

A method for inhibiting the spread of nosocomial infections in institutional health care settings comprises treating outer garments, worn indoors by employed staff of the institution, to impart antimicrobial properties to those garments by immersing the garments in a solution of glyxol, eugenol and water, squeezing the solution out of the garments, curing the wetted garments under heat, and drying the cured garments; and thereafter requiring employed staff to wear the treated garments while working at the institution; laundering the garments after being worn by the staff, for further wear by the staff, and requiring employed staff to wear the treated garments after the garments have been laundered for so long as the garments retain their antimicrobial properties.


Patent
Philadelphia University | Date: 2016-01-07

A method for removing airborne bacteria and airborne mycetes from indoor air comprises imparting microbiocidal and microbiostatic properties to fabric by treating the fabric with one or more naturally occurring biocides, positioning the treated fabric in a sheet-like orientation, and blowing indoor air through the fabric.


Patent
Philadelphia University | Date: 2016-01-04

Methods and apparatus for combating sick building syndrome include a plenum that is at least partially bounded by fabric comprising at least one naturally occurring botanically based, antimicrobial, morbidity-inducing fabric and preferably a fan for introducing air into the plenum for passage outwardly through the fabric thereby to cleanse the air of microbes contributing to sick building syndrome.


Patent
Philadelphia University | Date: 2014-03-17

A method for making an antibacterial fabric having resistance to laundering while maintaining its antibacterial properties.

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