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Āzamgarh, India

Hossain E.,Pharmacy College | Chandra G.,University of Burdwan | Nandy A.P.,University of Burdwan | Mandal S.C.,Jadavpur University | Gupta J.K.,Jadavpur University
Parasitology Research | Year: 2012

Bombax malabaricum (family Bombacaceae) is used as anthelmintic in traditional system of medicine in Southern Punjab of Pakistan. The objective of this study was to evaluate the anthelmintic activity of the methanol extract of B. malabaricum leaves (MEBM). Live parasites (trematode: Paramphistomum explanatum) were collected from buffalo in 0.9% phosphate-buffered saline. It was incubated in Petri dishes at 37±1°C in media containing either no extract (control) or MEBM, the test drug at 10, 25, 50, and 100 mg/ml dose level or albendazole, the standard drug at 10 mg/ml. The efficacy of the extract or albendazole was measured on the basis of the loss of spontaneous movement and/or death of the trematodes. Paralysis was considered when there is no movement unless shaken vigorously. Death was confirmed when the trematodes completely lost their motility, even when vigorously shaken or dipped in warm water (50°C), followed by fading away of their body color. The trematodes, both drug treated and others, were further processed for SEM study using the standard method. All trematodes died with all the abovementioned doses of MEBM within a short period of time (less than 45 min) which was statistically highly significant (p<0.001). MEBM at 100 mg/ml showed maximum efficacy. It paralyzed and killed trematodes in 18.50±0.62 and 22.17±0.48 min, respectively. SEM study showed that MEBM-treated trematodes were stretched. The study established the anthelmintic activity of MEBM. © Springer-Verlag 2011.

Jiladia M.A.,Veerayatan Institute of Pharmacy | Pandya S.S.,Pharmacy College | Jiladia A.G.,Jiladia Hospital
International Journal of Pharma and Bio Sciences | Year: 2010

A simple and sensitive, HPLC method has been developed and validated for the quantitative estimation of repaglinide in its single component tablet formulations (2 mg). Determination was performed using a Thermo BDS C18 column with mobile phase phosphate buffer: acetonitrile (pH 6.0; 45:55, v/v), and ultraviolet (UV) detection at 288 nm. The method was validated in terms of linearity (10-60 μg/ml), precision (intra-day variation below 0.6, inter-day variation below 0.2), accuracy (97.9 to 100.1%), ruggedness (within 0.5%) and specificity. The limit of detection and limit of quantification for repaglinide were found to be 1 μg/ml and 3 μg/ml, respectively. The developed method was successfully used for the assay of repaglinide tablet formulations. The method is simple, sensitive and precise; it can be used for the routine quality control testing of marketed formulations.

Samanta K.,Pharmacy College
Research Journal of Pharmaceutical, Biological and Chemical Sciences | Year: 2012

Hygrophila difformis Blume (Family-Acanthaceae) and has great medicinal importance like An antibacterial activity, high insecticide activity and Tribal's are also used as anticoagulants. It is very useful in tropical fresh water aquarium plant and Environmental importance Ornamentals plants. In present study deals with the characterization of morphological features, determination of physical constant such as the total ash value, acid insoluble ash value and water soluble ash value were 1.97%, 0.59%, 1.37% respectively. Loss of weight drying was 4.5%, the percent yield for ethanol 9.12% and aqueous 5.45%.

Hossain E.,Pharmacy College | Rawani A.,University of Burdwan | Chandra G.,University of Burdwan | Mandal S.C.,Jadavpur University | Gupta J.K.,Jadavpur University
Asian Pacific Journal of Tropical Medicine | Year: 2011

Objective: To analyze the larvicidal activity of two plant leaf powder and leaf-extracts, Dregea volubilis and Bombax malabaricum against Culex quinquefasciatus. Methods: The larvicidal bioassay was done for powdered leaves of Dregea volubilis and Bombax malabaricum individually and their methanol extracts against first-, second-, third- and fourth-instar larval forms of Culex quinquefasciatus. Mortality rate was recorded after 24, 48 and 72 h of post-exposure. LC 50 and LC 90 values were calculated at different time intervals for third instar larvae. Results: All the graded concentration (0.1%, 0.2%, 0.3%, 0.4%, 0.5%) of powdered leaves showed significant (P <0.05) larval mortality. The mortality rate was higher in 50 ppm doses of methanolic extracts of both the plants against Culex quinquefaciatus. The corresponding LC 50 values were 56.97 ppm and 48.85 ppm, respectively after 24 h of exposure. There is no mortality of non-target organism such as Chironomous circumdatus, Oreochromis niloticus niloticus and Diplonichus annulatum within 72 h of post exposure to 0.1%, 0.2%, 0.3% crude powdered leaves and methanolic extracts of both the two individual plants under the laboratory condition. The results of preliminary qualitative phytochemical analysis of both the plants revealed the presence of many bioactive principles such as steroids, tannins, flavonoids, triterpenoids, saponins, etc. that may be responsible for their bio-control potentiality. Conclusions: The results have shown potential and eco-friendly use of both plant extracts against larva of Culex quinquefasciatus for the first time. © 2011 Hainan Medical College.

Lei G.M.,Pharmacy College
Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials | Year: 2011

To improve the quality standard of stewed Rhizoma Polygonati with yellow wine. On the basis of Chinese Pharmacopoeia 2010, some items were added, which included assay of reducing sugars, water-soluble extracts, acid insoluble ash content and 5-hydroxymethylfurfural. Reducing sugars was no less than 34.0%, water-soluble extracts no less than 19.0%, acid insoluble ash content no more than 0.2%, 5-hydroxymethylfurfural no more than 0.02%. The quality standard of stewed Rhizoma Polygonati with yellow wine is improved on the basis of Chinese Pharmacopoeia.

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