Pharmacognosy and Ethnopharmacology Division

India

Pharmacognosy and Ethnopharmacology Division

India
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Kumar B.,Pharmacognosy and Ethnopharmacology Division | Shukla P.K.,Pharmacognosy and Ethnopharmacology Division | Niranjan A.,Pharmacognosy and Ethnopharmacology Division | Misra A.,CSIR - Central Electrochemical Research Institute | And 2 more authors.
Indian Journal of Traditional Knowledge | Year: 2017

Biophytum sensitivum (L.) DC. (Oxalidaceae) is a widely used medicinal plant in traditional medicinal systems across the globe. The present study deals with the RP-HPLC quantification of five phenolic acids, along with the in vitro antioxidant and antidiabetic activity of the aerial parts of the plant as a scientific investigation of traditional claims of its use as an antioxidant and antidiabetic agent. Anatomy of the stem was done to aid in exploring identification parameters for this plant. Among the five identified phenolic markers, caffeic acid exhibits the highest concentration (352.47 μg/gm), followed by ferulic acid (242.28 μg/gm), gallic acid (233.55 μg/gm), chlorogenic acid (192.06 μg/gm), and rutin (64.67 μg/gm). The total phenolic and flavonoid contents in the methanolic extract were found to be 87.0 ± 0.404 mg/gm GAE and 14.268 ± 0.055 mg/gm QE, respectively. The IC50 value for the in vitro DPPH method was 0.164 ± 0.411 mg/ml. In vitro antidiabetic activity was analyzed by the starch-iodine assay and the 3,5-DNS method which displayed IC50 values of 0.636 ± 0.05 mg/ml and 1.214 ± 0.04 mg/ml, respectively. The in vitro study results suggest promising antioxidant and antidiabetic activity of the plant which supports its use in the traditional systems of medicine. © 2016, All Rights Reserved.


Misra A.,Pharmacognosy and Ethnopharmacology Division | Srivastava S.,Pharmacognosy and Ethnopharmacology Division | Srivastava P.,CSIR - Central Electrochemical Research Institute | Shukla P.,Pharmacognosy and Ethnopharmacology Division | And 2 more authors.
Industrial Crops and Products | Year: 2016

Phytogeographical variation has an impact on up/down regulation of secondary metabolites in medicinal plants. In the same context, present study was planned to evaluate the variation of forskolin content in Coleus forskohlii from Vidarbha region (Maharashtra), India having different topographical conditions. The chemotypic variation was further correlated with existing soil profile. The data revealed the similarity in morphological, macroscopic and microscopic characters of collected germplasms but there is significant (p < 0.05) variation in the content of chemical marker, forskolin. Physicochemical and phytochemical parameters are within the limits of API and serve as standard for commercial utility of samples. NBC-29 (Narkhed) and NBC-31 (Katol) are the first two high forskolin yielding samples, which are clustered together on the basis of forskolin content with similar soil profiles. Furthermore our study demonstrated that there is significant variation in available NPK and micronutrient content within the soil samples and may be reasoned for existing chemotypic variability in samples. Among the micronutrients, manganese and copper exhibit the positive correlation with forskolin.Thus, elite chemotype of C. forskohlii were identified among the samples collected from Vidarbha region and express the relevance of phyto-geographical conditions on the quantity of industrially viable metabolites, forskolin. This aids in site specific exploration of high metabolite yielding accession/samples to meet out the commercial demands and, supply and to promote the agriculture practices of this industrial crop for societal upliftment in area having similar phytogeographical locations. © 2016 Elsevier B.V.

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