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Sadraeian M.,Pharmaceutical science Research Center | Mansoorkhani M.J.K.,Shiraz University of Medical Sciences | Mohkam M.,Pharmaceutical science Research Center | Ghasemi Y.,Shiraz University of Medical Sciences
International Journal of Gynecological Cancer | Year: 2013

Objective: In immunotherapy of HPV-16Yassociated cervical cancers, the E7 protein is considered as a prime candidate. However, it is a poor inducer of a cytotoxic T-cell response when used as a singular antigen in protein vaccination. Therefore, to design effective cancer vaccines, the best tumor antigens should be combined with the most effective immunogens or drug delivery tools to achieve positive clinical results. In this study, we fused HPV-16 E7 with the lectin subunit of ricin toxin (RTB) from castor plant as a vaccine adjuvant/carrier. Materials and Methods: After reaching the soluble form of the recombinant protein, we designed 2 preventive and inhibition tumor models for investigation of the prevention and rejection of TC-1 cell growth in female C57BL/6 mice, respectively. In each model, mice were immunized with the recombinant protein of E7-RTB or E7 without any adjuvant. Results: We demonstrated that prophylactic immunization of E7-RTB protected mice against challenge from TC-1 cells. Also in the therapeutic model, E7-RTB could inhibit TC-1 tumor growth in the lung. The results were significant compared with the immunization of E7 singularly. Conclusions: We concluded that immunization with E7-RTB protein without any adjuvant could generate antitumor effects in mice challenged with TC-1 cells. This research verifies the clinical applications and the future prospects for development of HPV-16 E7 therapeutic vaccines fused to immunoadjuvants. © 2013 by IGCS and ESGO.


Almasirad A.,Islamic Azad University at Tehran | Samiee-Sadr S.,Islamic Azad University at Tehran | Shafiee A.,Pharmaceutical science Research Center
Iranian Journal of Pharmaceutical Research | Year: 2011

A series of new 2-(phenylthio) benzoylaryl hydrazones were synthesized by acid-catalyzed condensation of hydrazide 3 with corresponding aldehydes. The chemical structures of the compounds were elucidated by FT-IR, 1H-NMR and Mass spectra. All newly synthesized compounds were evaluated for their antimycobacterial activities against Mycobacterium tuberculosis H37Rv using the microplate alamar blue assay (MABA). Compounds 4f (5-Nitro-2-furyl analogue) and 4g (5-Nitro-2-thienyl analogue) showed antimycobacterial activity with IC 90, 7.57 and 2.96 μg/mL, respectively. © 2011 by School of Pharmacy.


Norouzi P.,University of Tehran | Norouzi P.,Tehran University of Medical Sciences | Gupta V.K.,Indian Institute of Technology Roorkee | Gupta V.K.,King Fahd University of Petroleum and Minerals | And 6 more authors.
Analytical Chemistry | Year: 2011

In this work, a highly sensitive carcinoembryonic antigen fast Fourier transform admittance biosensor is introduced. The proposed biosensor is based on bilayer films of ZnO/Au nanoparticles as an immobilization matrix. These layers are prepared by self-assembly and deposition method on a gold electrode surface, respectively. Carcinoembryonic antibody (anti-CEA) was immobilized on gold nanoparticles and positively charged horseradish peroxidase (HRP) was used to block sites against nonspecific binding. The admittance biosensor was developed based on fast Fourier transform continuous square wave voltammetry, which produces a sensitive, fast (less than 20 s) and reliable response for determination of carcinoembryonic antigen. The technique was applied as a detector in a flow injection system. The admittances reduction current of the biosensor decreases linearly in two concentrations ranges of CEA from 0.1 to 70 ng/mL and from 70 to 200 ng/mL with a detection limit of 0.01 ng/mL in presence of 0.5 mM H2O2 as an eluent solution. © 2011 American Chemical Society.


Faridbod F.,Tehran University of Medical Sciences | Zamani H.A.,Islamic Azad University at Qūchān | Hosseini M.,University of Tehran | Pirali-Hamedani M.,Tehran University of Medical Sciences | And 3 more authors.
International Journal of Electrochemical Science | Year: 2011

Using multi-walled carbon nanotube (MWCNT) and ionic liquid (IL) in the composition of carbon paste electrodes cause improvement in their characterizations. Previous solution studies showed a selective interaction between N,N-bis(a-methylsalicylidene)diethylenetriamine (BMT) and Pr3+ ions respect to other lanthanide ions. To have an electrode with enhanced mechanical resistant in analysis, BMT incorporation of MWCNTs and ionic liquid in carbon paste was used in construction of a Pr3+ potentiometric electrode. The nano-composite electrode showed better sensitivity, selectivity, response time, response stability and lifetime in comparison with typical carbon paste electrodes. The best performance for nano-composite sensor was obtained with electrode composition of 25% BMT, 25% IL, 45% graphite powder, and 5% MWCNT. The new electrode exhibited a Nernstian response (20.1±0.4 mV per decade) toward Pr3+ ions in the range of 1.0×10-6-1.0×10-2 mol L-1. © 2011 by ESG.


Norouzi P.,University of Tehran | Pirali-Hamedani M.,Tehran University of Medical Sciences | Pirali-Hamedani M.,Pharmaceutical science Research Center | Ranaei-Siadat S.O.,Tehran University of Medical Sciences | Ganjali M.R.,University of Tehran
International Journal of Electrochemical Science | Year: 2011

It was found that N-[(Z)-1-(2-thienyl)methylidene]-N-[4-(4-{[(Z)-1-(2-thienyl)methylidene] amino}benzyl)phenyl] amine (TBPA) can form a selective complex with lutetium ions respect to other lanthanide ions based on our previous study. To have a long-term stable electrode for potentiometric uses, TBPA was applied as an ionophore in preparation of a nanocomposite carbon paste electrode. The pastes were prepared using functionalized multi-walled carbon nanotube (MWCNT-NH2), nanosilica (NS), graphite, and room temperature ionic liquid (RTIL). The carbon paste electrode composed of 5% MWCNT-NH2, 1%NS, 25% TBPA, 20% RTIL, and 49% graphite powder showed the best response. Nernstian response of (19.8±0.2 mV decade-1) in the concentration range of 1.0× 10-6-1.0×10-2 M with a detection limit of 9.5×10-7 M was achieved. The response of the electrode was pH independent in the range of 3.5-9.0. The proposed Lu(III) nano-composite carbon paste sensor displayed good selectivity, fast response time and long lifetime. © 2011 by ESG.


Ganjali M.R.,University of Tehran | Hosseini M.,University of Tehran | Pirali-Hamedani M.,Tehran University of Medical Sciences | Pirali-Hamedani M.,Pharmaceutical science Research Center | Zamani H.A.,Islamic Azad University at Qūchān
International Journal of Electrochemical Science | Year: 2011

Given the results of our previous studies indicating a relatively strong interaction between 2-{[(6-aminopyridin-2-yl) imino] methyl}-phenol (APIMP) and Nd(III) ions, the ligand was incorporated, as the sensing material, into a nano-composite carbon paste electrodes which were prepared using functionalized multi-walled carbon nanotube (MWCNT-NH2), nanosilica (NS), graphite, and an room temperature ionic liquid namely 1-n-butyl-3-methylimidazolium tetrafluoroborate [bmim]BF4 (RTIL). The electrodes composed of 2%NS, 5% MWCNT-NH2, 20% APIMP, 20% RTIL, and 53% graphite powder were found to show the optimum behavior. The sensor shows a Nernstian response of (19.8±0.12 mV decade-1) in the concentration window of 1.0×10-6-1.0×10-2 M with a detection limit of 8.0×10-7 M. The response of the sensor was found to be stable in the pH of 4.0-8.0 and the nano-composite based Nd(III) sensors displayed good selectivity with respect to a number of lanthanide and transition metal ions as well as a rather long lifetime. © 2011 by ESG.


Faridbod F.,Tehran University of Medical Sciences | Ganjali M.R.,Tehran University of Medical Sciences | Ganjali M.R.,University of Tehran | Pirali-Hamedani M.,Tehran University of Medical Sciences | And 3 more authors.
International Journal of Electrochemical Science | Year: 2010

Fluorescence studies in acetonitrile solution proved a strong interaction between N'-(1-oxoacenaphthylen-2(1H)-ylidene) furan-2-carbohydrazide (L) with Yb3+ ions in comparison with other lanthanide ions. Thus, L can be used as a suitable ionophore in construction of the Yb3+ potentiometric sensor. To have an electrode with enhanced mechanical resistant in real samples, L was used as a sensing material in new composite carbon paste electrode. The carbon paste were made based on a new nano-composite including multi-wall carbon nanotube (MWCNT), and room temperature ionic liquid, 1-n-butyl-3-methylimidazolium tetrafluoroborate [bmim]BF4. The nano-composite electrode showed better sensitivity, selectivity, response time, response stability and lifetime in comparison with typical carbon paste electrodes. The best performance for nano-composite sensor was obtained with electrode composition of 25% L, 25% [bmim]BF4, 45% graphite powder, and 5% MWCNT. The new electrode exhibited a Nernstian response (19.6±0.3 mV per decade) toward Yb3+ ions in the range of 1.0×10-8-1.0×10-2 mol L-1. © 2010 by ESG.


Norouzi P.,University of Tehran | Norouzi P.,Tehran University of Medical Sciences | Pirali-Hamedani M.,Tehran University of Medical Sciences | Pirali-Hamedani M.,Pharmaceutical science Research Center | And 3 more authors.
International Journal of Electrochemical Science | Year: 2010

In this paper, a new flow injection enzymatic acetylcholinesterase (AChE) biosensor was designed for selective determination of monocrotophos (an organophosphate pesticide). The biosensor was constructed by modifying glassy carbon electrode surface with gold nanoparticles (AuNPs) and multiwall carbon nanotubes (MWCNTs) while chitosan microspheres used to immobilize AChE. The measurement method was based on fast Fourier transform continuous cyclic voltammetry (FFTCCV) in which the charge under the peak calculated in a specific potential range. The characterization of the biosensor was studied by Scanning Electron Microscopy (SEM) and electrochemical impedance spectroscopy methods (ESI). The combination of MWCNTs and AuNPs promotes the electron transfer and catalyzes the electro-oxidation of thiocholine, and causes the amplifying the detection sensitivity. The inhibition of the enzyme activity by monocrotophos was proportional to monocrotophos concentration in the range of 0.1 to 10 μM, with a detection limit of 10 nM. Experimental parameters of measurement which affect the sensitivity of the biosensors, including potential scan rate, solution flow rate and buffer pH were optimized. The proposed biosensor shows good reproducibility, long-term storage stability and accuracy in analysis. © 2010 by ESG.


Ahmadkhaniha R.,Pharmaceutical science Research Center | Shafiee A.,Pharmaceutical science Research Center | Rastkari N.,Tehran University of Medical Sciences | Khoshayand M.R.,Tehran University of Medical Sciences | Kobarfard F.,Tehran University of Medical Sciences
Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences | Year: 2010

Providing "real blank sample" is a problem in determination of endogenous steroids in complex matrices. A new quantification strategy is proposed in the present study, which is based on using isotope-labeled steroids instead of natural steroids for constructing calibration line. This approach is called surrogate analyte and it is shown that its accuracy is better than some of the previously described methods at low concentrations and comparable to standard addition method at medium and high concentration levels. The method was fully validated to satisfy the ICH criteria and it was applied for determination of endogenous steroids in several urine samples. © 2010 Elsevier B.V. All rights reserved.


Rastkari N.,Tehran University of Medical Sciences | Ahmadkhaniha R.,Pharmaceutical science Research Center | Samadi N.,Biotechnology Research Center | Shafiee A.,Pharmaceutical science Research Center | Yunesian M.,Tehran University of Medical Sciences
Analytica Chimica Acta | Year: 2010

Carbon nanotubes are a kind of new carbon-based nanomaterials, which have drawn great attention in many application fields. The potential of single-walled carbon nanotubes (SWCNTs) as solid-phase microextraction (SPME) adsorbent for the preconcentration of environmental pollutants has been investigated in recent years. In the present study, the feasibility of SWCNTs as SPME adsorbent for the determination of monobutyltin, dibutyltin and tributyltin in seawater samples was studied. To achieve this aim, the potential factors affecting the SPME efficiency, including extraction time, extraction temperature, desorption time, desorption temperature, and salinity were optimized. The developed method showed good performance according to the ICH (International Conference on Harmonization of Technical Requirements for Analytical Methods) criteria. The acquired calibration curves were linear (r ≥ 0.992) over the concentration range from ≤12 to 2000 ng L-1. For all of the analytes, the limit of detection at signal-to-noise ratio of 3 was below 5 ng L-1. Furthermore, in comparison with the commercial carboxen/polydimethylsiloxane fiber, the developed SWCNT fiber showed better thermal stability (over 350 °C) and longer life span (over 150 times). The application of the proposed method in environmental analyses was shown by analyzing seawater samples from the harbors on the Persian Gulf for butyltin residues. Some of the butyltins were detected in the analyzed samples. Results of the present study demonstrate the feasibility of the SWCNTs as SPME adsorbent for the determination of butyltins in seawater samples. © 2009 Elsevier B.V. All rights reserved.

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