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Rahimi R.,Tehran University of Medical Sciences | Nikfar S.,TUMS and Iranian Food and Drug Organisation | Abdollahi M.,Pharmaceutical science Research Center
International Journal of Pharmacology | Year: 2013

The use of herbal medicine for the management of Inflammatory Bowel Disease (IBD) is increasing. The aim of the present study is to compare the efficacy and tolerability of herbal medicines with 5-aminosalicylates (5-ASAs) in IBD by conducting a meta-analysis. For this purpose, electronic databases were searched for studies comparing efficacy and/or tolerability of herbal medicines with 5-ASAs in different types of IBD. The search terms were: "herb" or "plant" or "herbal" and "inflammatory bowel disease". Data were collected from 1966-2013 (up to Feb). The "clinical response", "clinical remission", "endoscopic response", "endoscopic remission", "histological response", "histological remission", "relapse", "any adverse events" and "serious adverse events" were the key outcomes of interest. Eight placebo controlled clinical trials met criteria and were included. Comparison of herbal medicine with 5-ASAs yielded the following results: a significant Relative Risk (RR) of 1.28 (95% Confidence Interval (CI): 1.07-1.54, p = 0.008) for clinical remission; a significant RR of 1.19 (95% Cl = 1.01-1.39, p = 0.04) for clinical response; a non-significant RR of 0.85 (95% Cl: 0.34-2.12, p = 0.73) for endoscopic remission; a non-significant RR of 1.14 (95% Cl: 0.99-1.3, p = 0.07) for endoscopic response; a non-significant RR of 0.8 (95% Cl: 0.05-13.72) for histological remission; a non-significant RR of 1.32 (95% Cl: 0.64-2.9) for histological response; a non-significant RR of 1.05 (95% Cl: 0.6-1.83, p = 0.87) for relapse; a non-significant RR of 1.31 (95% Cl: 0.8-2.14, p = 0.28) for any adverse events; and a non-significant RR of 1.8 (95% Cl: 0.13-24.5, p = 0.66) for serious adverse events. Overall, the efficacy and tolerability of herbal medicines in IBD is comparable to 5-ASAs, but the evidence is too limited to make any confident conclusion. Further high quality, large controlled trials are still needed.


Sadraeian M.,Pharmaceutical science Research Center | Mansoorkhani M.J.K.,Shiraz University of Medical Sciences | Mohkam M.,Pharmaceutical science Research Center | Ghasemi Y.,Shiraz University of Medical Sciences
International Journal of Gynecological Cancer | Year: 2013

Objective: In immunotherapy of HPV-16Yassociated cervical cancers, the E7 protein is considered as a prime candidate. However, it is a poor inducer of a cytotoxic T-cell response when used as a singular antigen in protein vaccination. Therefore, to design effective cancer vaccines, the best tumor antigens should be combined with the most effective immunogens or drug delivery tools to achieve positive clinical results. In this study, we fused HPV-16 E7 with the lectin subunit of ricin toxin (RTB) from castor plant as a vaccine adjuvant/carrier. Materials and Methods: After reaching the soluble form of the recombinant protein, we designed 2 preventive and inhibition tumor models for investigation of the prevention and rejection of TC-1 cell growth in female C57BL/6 mice, respectively. In each model, mice were immunized with the recombinant protein of E7-RTB or E7 without any adjuvant. Results: We demonstrated that prophylactic immunization of E7-RTB protected mice against challenge from TC-1 cells. Also in the therapeutic model, E7-RTB could inhibit TC-1 tumor growth in the lung. The results were significant compared with the immunization of E7 singularly. Conclusions: We concluded that immunization with E7-RTB protein without any adjuvant could generate antitumor effects in mice challenged with TC-1 cells. This research verifies the clinical applications and the future prospects for development of HPV-16 E7 therapeutic vaccines fused to immunoadjuvants. © 2013 by IGCS and ESGO.


Duty S.,King's College London | Jenner P.,Pharmaceutical science Research Center
British Journal of Pharmacology | Year: 2011

Animal models of Parkinson's disease (PD) have proved highly effective in the discovery of novel treatments for motor symptoms of PD and in the search for clues to the underlying cause of the illness. Models based on specific pathogenic mechanisms may subsequently lead to the development of neuroprotective agents for PD that stop or slow disease progression. The array of available rodent models is large and ranges from acute pharmacological models, such as the reserpine- or haloperidol-treated rats that display one or more parkinsonian signs, to models exhibiting destruction of the dopaminergic nigro-striatal pathway, such as the classical 6-hydroxydopamine (6-OHDA) rat and 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) mouse models. All of these have provided test beds in which new molecules for treating the motor symptoms of PD can be assessed. In addition, the emergence of abnormal involuntary movements (AIMs) with repeated treatment of 6-OHDA-lesioned rats with L-DOPA has allowed for examination of the mechanisms responsible for treatment-related dyskinesia in PD, and the detection of molecules able to prevent or reverse their appearance. Other toxin-based models of nigro-striatal tract degeneration include the systemic administration of the pesticides rotenone and paraquat, but whilst providing clues to disease pathogenesis, these are not so commonly used for drug development. The MPTP-treated primate model of PD, which closely mimics the clinical features of PD and in which all currently used anti-parkinsonian medications have been shown to be effective, is undoubtedly the most clinically-relevant of all available models. The MPTP-treated primate develops clear dyskinesia when repeatedly exposed to L-DOPA, and these parkinsonian animals have shown responses to novel dopaminergic agents that are highly predictive of their effect in man. Whether non-dopaminergic drugs show the same degree of predictability of response is a matter of debate. As our understanding of the pathogenesis of PD has improved, so new rodent models produced by agents mimicking these mechanisms, including proteasome inhibitors such as PSI, lactacystin and epoximycin or inflammogens like lipopolysaccharide (LPS) have been developed. A further generation of models aimed at mimicking the genetic causes of PD has also sprung up. Whilst these newer models have provided further clues to the disease pathology, they have so far been less commonly used for drug development. There is little doubt that the availability of experimental animal models of PD has dramatically altered dopaminergic drug treatment of the illness and the prevention and reversal of drug-related side effects that emerge with disease progression and chronic medication. However, so far, we have made little progress in moving into other pharmacological areas for the treatment of PD, and we have not developed models that reflect the progressive nature of the illness and its complexity in terms of the extent of pathology and biochemical change. Only when this occurs are we likely to make progress in developing agents to stop or slow the disease progression. The overarching question that draws all of these models together in the quest for better drug treatments for PD is how well do they recapitulate the human condition and how predictive are they of successful translation of drugs into the clinic? This article aims to clarify the current position and highlight the strengths and weaknesses of available models. © 2011 The British Pharmacological Society.


Almasirad A.,Islamic Azad University at Tehran | Samiee-Sadr S.,Islamic Azad University at Tehran | Shafiee A.,Pharmaceutical science Research Center
Iranian Journal of Pharmaceutical Research | Year: 2011

A series of new 2-(phenylthio) benzoylaryl hydrazones were synthesized by acid-catalyzed condensation of hydrazide 3 with corresponding aldehydes. The chemical structures of the compounds were elucidated by FT-IR, 1H-NMR and Mass spectra. All newly synthesized compounds were evaluated for their antimycobacterial activities against Mycobacterium tuberculosis H37Rv using the microplate alamar blue assay (MABA). Compounds 4f (5-Nitro-2-furyl analogue) and 4g (5-Nitro-2-thienyl analogue) showed antimycobacterial activity with IC 90, 7.57 and 2.96 μg/mL, respectively. © 2011 by School of Pharmacy.


Norouzi P.,University of Tehran | Norouzi P.,Tehran University of Medical Sciences | Gupta V.K.,Indian Institute of Technology Roorkee | Gupta V.K.,King Fahd University of Petroleum and Minerals | And 6 more authors.
Analytical Chemistry | Year: 2011

In this work, a highly sensitive carcinoembryonic antigen fast Fourier transform admittance biosensor is introduced. The proposed biosensor is based on bilayer films of ZnO/Au nanoparticles as an immobilization matrix. These layers are prepared by self-assembly and deposition method on a gold electrode surface, respectively. Carcinoembryonic antibody (anti-CEA) was immobilized on gold nanoparticles and positively charged horseradish peroxidase (HRP) was used to block sites against nonspecific binding. The admittance biosensor was developed based on fast Fourier transform continuous square wave voltammetry, which produces a sensitive, fast (less than 20 s) and reliable response for determination of carcinoembryonic antigen. The technique was applied as a detector in a flow injection system. The admittances reduction current of the biosensor decreases linearly in two concentrations ranges of CEA from 0.1 to 70 ng/mL and from 70 to 200 ng/mL with a detection limit of 0.01 ng/mL in presence of 0.5 mM H2O2 as an eluent solution. © 2011 American Chemical Society.


Norouzi P.,University of Tehran | Pirali-Hamedani M.,Tehran University of Medical Sciences | Pirali-Hamedani M.,Pharmaceutical science Research Center | Ranaei-Siadat S.O.,Tehran University of Medical Sciences | Ganjali M.R.,University of Tehran
International Journal of Electrochemical Science | Year: 2011

It was found that N-[(Z)-1-(2-thienyl)methylidene]-N-[4-(4-{[(Z)-1-(2-thienyl)methylidene] amino}benzyl)phenyl] amine (TBPA) can form a selective complex with lutetium ions respect to other lanthanide ions based on our previous study. To have a long-term stable electrode for potentiometric uses, TBPA was applied as an ionophore in preparation of a nanocomposite carbon paste electrode. The pastes were prepared using functionalized multi-walled carbon nanotube (MWCNT-NH2), nanosilica (NS), graphite, and room temperature ionic liquid (RTIL). The carbon paste electrode composed of 5% MWCNT-NH2, 1%NS, 25% TBPA, 20% RTIL, and 49% graphite powder showed the best response. Nernstian response of (19.8±0.2 mV decade-1) in the concentration range of 1.0× 10-6-1.0×10-2 M with a detection limit of 9.5×10-7 M was achieved. The response of the electrode was pH independent in the range of 3.5-9.0. The proposed Lu(III) nano-composite carbon paste sensor displayed good selectivity, fast response time and long lifetime. © 2011 by ESG.


Ganjali M.R.,University of Tehran | Hosseini M.,University of Tehran | Pirali-Hamedani M.,Tehran University of Medical Sciences | Pirali-Hamedani M.,Pharmaceutical science Research Center | Zamani H.A.,Islamic Azad University at Qūchān
International Journal of Electrochemical Science | Year: 2011

Given the results of our previous studies indicating a relatively strong interaction between 2-{[(6-aminopyridin-2-yl) imino] methyl}-phenol (APIMP) and Nd(III) ions, the ligand was incorporated, as the sensing material, into a nano-composite carbon paste electrodes which were prepared using functionalized multi-walled carbon nanotube (MWCNT-NH2), nanosilica (NS), graphite, and an room temperature ionic liquid namely 1-n-butyl-3-methylimidazolium tetrafluoroborate [bmim]BF4 (RTIL). The electrodes composed of 2%NS, 5% MWCNT-NH2, 20% APIMP, 20% RTIL, and 53% graphite powder were found to show the optimum behavior. The sensor shows a Nernstian response of (19.8±0.12 mV decade-1) in the concentration window of 1.0×10-6-1.0×10-2 M with a detection limit of 8.0×10-7 M. The response of the sensor was found to be stable in the pH of 4.0-8.0 and the nano-composite based Nd(III) sensors displayed good selectivity with respect to a number of lanthanide and transition metal ions as well as a rather long lifetime. © 2011 by ESG.


Faridbod F.,Tehran University of Medical Sciences | Ganjali M.R.,Tehran University of Medical Sciences | Ganjali M.R.,University of Tehran | Pirali-Hamedani M.,Tehran University of Medical Sciences | And 3 more authors.
International Journal of Electrochemical Science | Year: 2010

Fluorescence studies in acetonitrile solution proved a strong interaction between N'-(1-oxoacenaphthylen-2(1H)-ylidene) furan-2-carbohydrazide (L) with Yb3+ ions in comparison with other lanthanide ions. Thus, L can be used as a suitable ionophore in construction of the Yb3+ potentiometric sensor. To have an electrode with enhanced mechanical resistant in real samples, L was used as a sensing material in new composite carbon paste electrode. The carbon paste were made based on a new nano-composite including multi-wall carbon nanotube (MWCNT), and room temperature ionic liquid, 1-n-butyl-3-methylimidazolium tetrafluoroborate [bmim]BF4. The nano-composite electrode showed better sensitivity, selectivity, response time, response stability and lifetime in comparison with typical carbon paste electrodes. The best performance for nano-composite sensor was obtained with electrode composition of 25% L, 25% [bmim]BF4, 45% graphite powder, and 5% MWCNT. The new electrode exhibited a Nernstian response (19.6±0.3 mV per decade) toward Yb3+ ions in the range of 1.0×10-8-1.0×10-2 mol L-1. © 2010 by ESG.


Ahmadkhaniha R.,Pharmaceutical science Research Center | Shafiee A.,Pharmaceutical science Research Center | Rastkari N.,Tehran University of Medical Sciences | Khoshayand M.R.,Tehran University of Medical Sciences | Kobarfard F.,Tehran University of Medical Sciences
Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences | Year: 2010

Providing "real blank sample" is a problem in determination of endogenous steroids in complex matrices. A new quantification strategy is proposed in the present study, which is based on using isotope-labeled steroids instead of natural steroids for constructing calibration line. This approach is called surrogate analyte and it is shown that its accuracy is better than some of the previously described methods at low concentrations and comparable to standard addition method at medium and high concentration levels. The method was fully validated to satisfy the ICH criteria and it was applied for determination of endogenous steroids in several urine samples. © 2010 Elsevier B.V. All rights reserved.


Rastkari N.,Tehran University of Medical Sciences | Ahmadkhaniha R.,Pharmaceutical science Research Center | Samadi N.,Biotechnology Research Center | Shafiee A.,Pharmaceutical science Research Center | Yunesian M.,Tehran University of Medical Sciences
Analytica Chimica Acta | Year: 2010

Carbon nanotubes are a kind of new carbon-based nanomaterials, which have drawn great attention in many application fields. The potential of single-walled carbon nanotubes (SWCNTs) as solid-phase microextraction (SPME) adsorbent for the preconcentration of environmental pollutants has been investigated in recent years. In the present study, the feasibility of SWCNTs as SPME adsorbent for the determination of monobutyltin, dibutyltin and tributyltin in seawater samples was studied. To achieve this aim, the potential factors affecting the SPME efficiency, including extraction time, extraction temperature, desorption time, desorption temperature, and salinity were optimized. The developed method showed good performance according to the ICH (International Conference on Harmonization of Technical Requirements for Analytical Methods) criteria. The acquired calibration curves were linear (r ≥ 0.992) over the concentration range from ≤12 to 2000 ng L-1. For all of the analytes, the limit of detection at signal-to-noise ratio of 3 was below 5 ng L-1. Furthermore, in comparison with the commercial carboxen/polydimethylsiloxane fiber, the developed SWCNT fiber showed better thermal stability (over 350 °C) and longer life span (over 150 times). The application of the proposed method in environmental analyses was shown by analyzing seawater samples from the harbors on the Persian Gulf for butyltin residues. Some of the butyltins were detected in the analyzed samples. Results of the present study demonstrate the feasibility of the SWCNTs as SPME adsorbent for the determination of butyltins in seawater samples. © 2009 Elsevier B.V. All rights reserved.

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