Yamamoto E.,Pharmaceutical Science and Technology Eisai Co. |
Hyodo K.,Pharmaceutical Science and Technology Eisai Co. |
Ohnishi N.,Pharmaceutical Science and Technology Eisai Co. |
Suzuki T.,Pharmaceutical Science and Technology Eisai Co. |
And 3 more authors.
Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences | Year: 2011
A method for the simultaneous measurement of liposome-encapsulated and released drugs in mouse plasma by on-line solid phase extraction (SPE)-SPE-HPLC with direct plasma injection was developed using a doxorubicin (DXR)-containing liposome formulation as the model drug. During SPE, the released DXR was extracted on the 1st restricted-access media (RAM) SPE column, whereas the liposomes were eluted. The eluted liposomes were collapsed on-line, and the released DXR was delivered to the 2nd RAM SPE column for extraction. The retained DXR on the SPE columns was analyzed via HPLC-fluorescent detector by switching the valves. The method was validated and applied to the pharmacokinetic study of DXR in mice after intravenous injection of DXR-containing liposomes. © 2011 Elsevier B.V.