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Ryszka F.,Medical Higher School in Sosnowiec | Ryszka F.,Pharmaceutical Research and Production Plant | Dolinska B.,Medical Higher School in Sosnowiec | Dolinska B.,Pharmaceutical Research and Production Plant | And 4 more authors.
Advances in Clinical and Experimental Medicine | Year: 2015

Background. It was previously found that synthetic, insect-derived octapeptide leucopyrokinin (LPK) applied directly into the lateral brain ventricle induced a significant antinociceptive effect in rats. Its synthetic truncated analog heptapeptide [2-8]-leucopyrokinin displayed a stronger antinociceptive effect in comparison to native LPK. Moreover it was previously found a high accumulation of these both 1251-labeled peptides in adrenals, as well as in hypothalamus and in hippocampus of rats brain. Objectives. The aim of the present study was to assess the distribution of 1251-labeled [2-8] -leucopyrokinin in rats' internal organs an in several parts of the brain after peripheral - intraperitoneal (i.p.) administration. Material and Methods. The study was performed on male Wistar rats. A synthetic [2-8]-leucopyrokinin ([2-8]- -LPK) was iodinated with Na125I. On the day of experiment a solution of 125I-[2-8]-LPK was i.p. injected and the next after 1 and 24 h animals were sacrificed by decapitation. Radioactivity levels in samples of parts of the brain and of internal organs were determined by counter Gamma Auto Count. Results. A uniform, low accumulation 1251-[2-8]-LPK was found in evaluated samples of the brain and in internal organs. Conclusions. The results of the present study indicate a weak penetration into the brain and internal organs of intraperitoneally applied 1251- [2-8] -LPK in rats and correspond with previously determined weak biological effects of i.p. injected LPK and [2-8]-LPK. © Copyright by Wroclaw Medical University.


PubMed | Pharmaceutical Research and Production Plant, Medical University of Silesia, Katowice and Wrocław University
Type: Journal Article | Journal: Advances in clinical and experimental medicine : official organ Wroclaw Medical University | Year: 2015

It was previously found that synthetic, insect-derived octapeptide leucopyrokinin (LPK) applied directly into the lateral brain ventricle induced asignificant antinociceptive effect in rats. Its synthetic truncated analog heptapeptide[2-8]-leucopyrokinin displayed astronger antinociceptive effect in comparison to native LPK. Moreover it was previously found ahigh accumulation of these both 125I-labeled peptides in adrenals, as well as in hypothalamus and in hippocampus of rats brain.The aim of the present study was to assess the distribution of 125I-labeled[2-8]-leucopyrokinin in rats internal organs an in several parts of the brain after peripheral -intraperitoneal (i.p.) administration.The study was performed on male Wistar rats. Asynthetic[2-8]-leucopyrokinin ([2-8]-LPK) was iodinated with Na125I. On the day of experiment asolution of 125I-[2-8]-LPK was i.p. injected and the next after 1 and 24 hanimals were sacrificed by decapitation. Radioactivity levels in samples of parts of the brain and of internal organs were determined by counter Gamma Auto Count.Auniform, low accumulation 125I-[2-8]-LPK was found in evaluated samples of the brain and in internal organs.The results of the present study indicate aweak penetration into the brain and internal organs of intraperitoneally applied 125I-[2-8]-LPK in rats and correspond with previously determined weak biological effects of i.p. injected LPK and[2-8]-LPK.


Dolinska B.,Pharmaceutical Research and Production Plant | Dolinska B.,University of Silesia | Mikulska A.,Pharmaceutical Research and Production Plant | Caban A.,Medical University of Silesia, Katowice | And 2 more authors.
Biological Trace Element Research | Year: 2011

An in vitro model was used to simulate the intestinal permeation of calcium ions depending on the type of salt (carbonate, fumarate, citrate, or gluconate), its concentration (1.0, 2.5, 5.0, or 10 mM/l), and pH (1.3, 4.2, 6.2, or 7.5). To simulate the conditions for calcium permeation in a patient in a fasting state, the solutions were placed in contact with segments of small intestine of pig: stomach, duodenum, jejunum, and ileum. The percent permeation, its rate, and half-time were measured in each case. In all cases, the maximum permeation was seen at 1 mM concentration, depending on pH: 100% for carbonate at pH 1.3; 82% for fumarate, pH 6.2; 79.5% for citrate at pH 4.2, and 81% for gluconate at pH 7.4. The maximum rate of permeation (% h -1) was also observed at 1 mM: 2.16 for carbonate at pH 1.3, 0.29 for fumarate at pH 6.2, 0.26 for citrate at pH 4.2, and 0.28 for gluconate at pH 7.4. The shortest half-time permeation (t 1/2, h) for 1 mM solutions depended also on pH (in parentheses): carbonate 0.3 (1.3), fumarate 2.4 (6.2), citrate 2.6 (4.2), and gluconate 2.5 (7.4). The results suggest that calcium carbonate and citrate can be recommended to patients with normal gastric acidity and hyperacidity while fumarate and gluconate to patients with hypoacidity. © The Author(s) 2010.


Cierpka L.,Medical University of Silesia, Katowice | Ryszka F.,Pharmaceutical Research and Production Plant | Dolinska B.,Pharmaceutical Research and Production Plant | Dolinska B.,Medical University of Silesia, Katowice | And 7 more authors.
Transplantation Proceedings | Year: 2014

Biolasol solution (Pharmaceutical Research and Production Plant "Biochefa," Sosnowiec, Poland) is a novel extracellular perfusion and ex vivo hypothermic kidney preservation solution. It ensures maintenance of homeostasis, reduces tissue edema, has low viscosity, and allows the graft to preserve structural and functional integrity. It minimizes ischemia-reperfusion damage.Methods. Perfundates from control and transplanted kidneys flushed with Biolasol or ViaSpan solutions (Arkas, Warszawa, Poland) were analyzed. Parameters of serum and urine collected from 12 pigs after auto-transplantation were also analyzed. Renal medulla was investigated for structural alterations by analyzing hematoxylin and eosin-stained slides. The mean survival time of pigs after the auto-transplantation procedure was the measure for the novel Biolasol solution effectiveness.Results. We observed a statistically significant decrease in marker enzyme levels alanine transaminase, aspartate transaminase, lactic dehydrogenase, and ions (Na and K) in pigs with grafts flushed with Biolasol. Histopathologic examination revealed that the renal cortex structure was not damaged after the use of Biolasol solution.Conclusions. Biolasol solution protects kidneys against ischemia damage and does not differ significantly from the "golden standard" ViaSpan solution. © 2014 Elsevier Inc. All rights reserved.


PubMed | Pharmaceutical Research and Production Plant
Type: Journal Article | Journal: Biological trace element research | Year: 2011

An in vitro model was used to simulate the intestinal permeation of calcium ions depending on the type of salt (carbonate, fumarate, citrate, or gluconate), its concentration (1.0, 2.5, 5.0, or 10mM/l), and pH (1.3, 4.2, 6.2, or 7.5). To simulate the conditions for calcium permeation in a patient in a fasting state, the solutions were placed in contact with segments of small intestine of pig: stomach, duodenum, jejunum, and ileum. The percent permeation, its rate, and half-time were measured in each case. In all cases, the maximum permeation was seen at 1mM concentration, depending on pH: 100% for carbonate at pH1.3; 82% for fumarate, pH6.2; 79.5% for citrate at pH4.2, and 81% for gluconate at pH7.4. The maximum rate of permeation (% h(-1)) was also observed at 1mM: 2.16 for carbonate at pH1.3, 0.29 for fumarate at pH6.2, 0.26 for citrate at pH4.2, and 0.28 for gluconate at pH7.4. The shortest half-time permeation (t (1/2), h) for 1mM solutions depended also on pH (in parentheses): carbonate 0.3 (1.3), fumarate 2.4 (6.2), citrate 2.6 (4.2), and gluconate 2.5 (7.4). The results suggest that calcium carbonate and citrate can be recommended to patients with normal gastric acidity and hyperacidity while fumarate and gluconate to patients with hypoacidity.

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