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Du G.,University of Macau | Xiao Y.,University of Sichuan | Yang H.-R.,Pharmaceutical Factory of Chengdu Huasun Group Inc. | Wang L.,Pharmaceutical Factory of Chengdu Huasun Group Inc. | And 2 more authors.
Journal of Separation Science | Year: 2012

A selective pressurized liquid extraction and gas chromatography coupled with triple quadrupole mass spectrometer method was developed for simultaneous determination of 52 pesticide residues in medicine and food dual-purpose herbs. The developed extraction method integrated extraction and cleanup processes for sample preparation. The sorbents, 5 g Florisil and 100 mg graphitized carbon black, were placed inside the extraction cell to remove matrix interferences. Optimized conditions of selective pressurized liquid extraction were ethyl acetate as extraction solvent, 120°C of extraction temperature, 6 min of static extraction time, 50% of flush volume extracted for two cycles. An ultra inert capillary GC-MS HP-5 UI column (20 m × 0.18 mm id, 0.18 μm) and column backflush system were used for the analysis. Multiple-reaction monitoring was employed for the quantitative analysis with electron ionization mode. All calibration curves showed good linearity (r2 > 0.995) within the test ranges. The average recoveries of most pesticides were from 81 to 118%. The validated method was successfully applied for the determination of pesticide residues in four herbs. The results indicate that selective pressurized liquid extraction and GC-MS/MS is a sensitive and reliable analytical method for the simultaneous determination of multiple pesticide residues in herbs. © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Wang L.,Pharmaceutical Factory of Chengdu Huasun Group Inc. | Yang H.R.,Pharmaceutical Factory of Chengdu Huasun Group Inc. | Xue J.T.,University of Sichuan | Ye L.M.,University of Sichuan | And 2 more authors.
Asian Journal of Chemistry | Year: 2015

The object of this research was to develop a method for rapid and simultaneous determination of the ginsenosides (Rg1), ginsenosides (Re), notoginsenosides (R1) and panaxtrol saponin of Panax notoginseng by near infrared spectroscopy. Calibration models are generated by performing partial least-squares regression and optimized individually by considering spectral range, spectral pretreatment methods and number of model factors. Calibration models were developed for Rg1, Re, R1 and panaxtrol saponin with root mean square errors of cross-validation (RMSECV) of 0.157, 0.022, 0.0546 and 0.213, and the correlation coefficient (R2) of 89.33, 91.10, 96.12 and 91.64 %, respectively. The established model was validated and showed it was fast, non-destructive and accurate. This method provides a new efficient approach for determining the active components in the complex system of Panax notoginseng. © 2015, Chemical Publishing Co. All rights reserved.

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