Pharmaceutical College

Yantai, China

Pharmaceutical College

Yantai, China
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Cui P.-M.,Pharmaceutical College | Shu L.,Pharmaceutical College | Liu F.,Pharmaceutical College | Hu P.,Pharmaceutical College | And 3 more authors.
Chinese Pharmaceutical Journal | Year: 2014

OBJECTIVE: To investigate the anti-tumor activity of a novel cyclophosphamide derivate 4, 6-diphenyl cyclophosphamide (9b) on cultured SK-OV3 (human ovarian cancer cell line) and 143B (human osteosarcoma cell line) cells in vitro aid S180 (mouse sarcoma cell line) and B16 (murine melanoma cell line) solid tumors in vivo, and its possible anti-tumor mechanism. METHODS: The inhibitory effects of 9b on SK-OV3 and 143B cells proliferation were analyzed by MTT assay in vitro. The effect of 9b on cell cycle distribution and apoptosis were evaluated by flow cytometry. To evaluate the anti-tumor effect of 9b in vivo, mouse model bearing inoculated S180 and B16 tumor were established. RESULTS: MTT assay revealed that 9b obviously suppressed SK-OV3 and 143B cells growth and proliferation in a dose-dependent manner within the concentration ranging from 3.7 to 300 Limol·L-1 and in a time-dependent manner from 24 to 72 h. The IC50 value of 9b was (71.27±1.08) μmol·L-1 for SK-OV3 cells, and (98.50±0.82) μmol·L-1 for 143B cells respectively when incubation for 48 h. The results of flow cytometry indicated that after treated for 48 h with different concentration of 9b, the ratios of 143 B cells in the G0/G1 phase and SK-OV3 cells in the G0/G1 phase and G2/M phase were significantly increased compared with control group (P<0.05, P<0.01), the apoptosis rate had significantly increased (P<0.05, P<0.01). 9b (3, 10 and 30 mg·kg-1·d-1) could significantly reduce tumor weight in the S180 and B16 solid tumor mouse model in vivo. CONCLUSION: 9b showed significantly anti-tumor activity both in vivo and in vitro, of which the mechanism might be associated with the change of cell cycle distribution and induction of tumor cell apoptosis.


Objective: Tamarindus indica (Family- Fabaceae) show various folkloric uses in treatment of various ailments such as rheumatisum, dysentery, jaundice etc. Aim: The research was conducted to investigate its phytoconstituients and various activity such as antimicrobial, analgesic & anti-inflammatary of AETIRE. Method: The antimicrobial activity was performed on 4 bacterial stains containing (B.subtilis, S.aureus, P. aeruginosa & E.coli) on AETIRE using Disc diffusion method. The Analgesic activity was tested by thermal and chemical induced pain through Hot plate and AAIWT. And carrageenan induced rat paw oedema model is used to evaluate antiinflammatory activity. Result: Phytoconstituients such as tannins, alkaloids, saponins, flavonoids and carbohydrates present in both the extract. The maximum zone of inhibition of about 21mm & 22mm was shown on B.subtilis strain by both the extract when compared with standard drug (Tetracycline & Gentamycin). In AAIWT and hot plate test the AETIRE of concentration (100, 200 mg/kg) produce significant dose-dependent inhibition of pain response with maximum 54.33% protection against acetic acid induced pain and about 74.83% inhibition against thermally induced pain by the aqueous extract 200mg. & the anti- inflammatory activity shown by AETIRE (100 & 200mg/kg) caused significant dose dependent inhibition of oedema with maximum 45.94% inhibition in the Carrageenan induced rat paw oedema by the AE. Conclusion: Therefore the AE of Tamarindus indica root was more effective in showing analgesic and anti-inflammatory activity when compared to the standard drug in each model while ethanol extract show effective antimicrobial activity. © 2017 Phcog.Net.


Das C.,Pharmaceutical College | Dash S.,Institute of Pharmacy and Technology | Sahu A.K.,Pharmaceutical College | Hota R.,Pharmaceutical College | Rout D.,Pharmaceutical College
Journal of Chemical and Pharmaceutical Research | Year: 2011

The methanol extract of Capparis zeylanica Linn. root was evaluated for its wound healing potential in two different types of wound models viz., incision and excision. Two dose levels (100, 200 mg/kg b.w.) of methanol extract of Capparis zeylanica root were tested by incision wound model. The 100 mg/kg produced mild wound healing activity whereas 200 mg/kg produced pronounced healing properties in the tested parameters, tensile strength and DNA content. Two concentrations, 1% and 2% were tested in the excision wound model. There was better healing property observed in 2% concentration when compared to povidone iodine ointment. It was confirmed by finding the parameters; percentage wound contraction, epithelisation time, collegen content and DNA content.


Ma X.-H.,Chengdu University of Traditional Chinese Medicine | Ma X.-H.,Chinese Academy of Sciences | Ma Y.,Chinese Academy of Sciences | Ma Y.,Beijing Normal University | And 11 more authors.
Molecules | Year: 2015

Secondary metabolites from plants play key roles in human medicine and chemical industries. Due to limited accumulation of secondary metabolites in plants and their important roles, characterization of key enzymes involved in biosynthetic pathway will enable metabolic engineering or synthetic biology to improve or produce the compounds in plants or microorganisms, which provides an alternative for production of these valuable compounds. Salvia miltiorrhiza, containing tanshinones and phenolic acids as its active compounds, has been widely used for the treatment of cardiovascular and cerebrovascular diseases. The biosynthetic analysis of secondary metabolites in S. miltiorrhiza has made great progress due to the successful genetic transformation system, simplified hairy roots system, and high-throughput sequencing. The cloned genes in S. miltiorrhiza had provided references for functional characterization of the post-modification steps involved in biosynthesis of tanshinones and phenolic acids, and further utilization of these steps in metabolic engineering. The strategies used in these studies could provide solid foundation for elucidation of biosynthetic pathways of diterpenoids and phenolic acids in other species. The present review systematically summarizes recent advances in biosynthetic pathway analysis of tanshinones and phenolic acids as well as synthetic biology and metabolic engineering applications of the rate-limiting genes involved in the secondary metabolism in S. miltiorrhiza. © 2015 by the authors; licensee MDPI, Basel, Switzerland.


Yan M.-M.,Pharmaceutical College | Wei G.-C.,Pharmaceutical College | Zhang L.-X.,Pharmaceutical College | Ma P.,Pharmaceutical College | And 2 more authors.
Chinese Pharmacological Bulletin | Year: 2012

Aim: To explore the effect and mechanism of trichomerol on rat hepatoma cell CBRH7919. Methods: The effects of rat clediseffect of hepatoma cell CBRH7919 on cell viability and cell growth were deceted by SRB method. The changes of cell cycle, cell apotosis and mitochondrial membrane potenetial were determined by flow cytometry. The morphological changes were observed under Laser scanning confocal microscope. Results: The effect of rat clediseffect of hepatoma cell CBRH7919 on cell viability and cell growth was time and concentration-dependent. After treated with trichomerol, the rat clediseffect of hepatoma cell CBRH7919 in S stage was increased and in G stage was reduced. Apoptotic rate was concentration-dependent. The rat clediseffect of hepatoma cells CBRH7919 showed marked apoptosis by Acridine orange. Conclusion: Trichomerol can inhibit the growth of rat hepatoma cell CBRH7919 and induce the apoptosis of cells in vitro.


Liu X.-Y.,Fujian Medical University | Zhang L.-J.,Pharmaceutical college | Chen Z.,Fujian Medical University | Liu L.-B.,Fujian Medical University
Neurological Research | Year: 2017

Objectives: The aim of this study was to elucidate the mechanism underlying the neuroprotective effects of the phosphatase and tensin homolog (PTEN) inhibitor, bisperoxovanadium-pic [bpV(pic)]. Methods: We determined the effects of bpV(pic) on amyloid-β-peptide-(25-35)-induced neurotoxicity, particularly intracellular reactive oxygen species (ROS) production and mitochondria-mediated apoptotic signaling, in a human neuroblastoma (SH-SY5Y) cell model. Results: We found that exposure of SH-SY5Y cells to amyloid β peptides (Aβ25-35) resulted in a significant reduction in cell viability accompanied by increased lactate dehydrogenase (LDH) release, elevated levels of intracellular ROS, and decreased superoxide dismutase (SOD) activities, all of which were reversed by co-treatment with bpV(pic). Moreover, bpV(pic) induced significant protection against Aβ25-35-induced apoptosis, and effectively suppressed mitochondria-dependent apoptotic signaling triggered by Aβ25-35. Discussion: Aβ peptides are thought to cause neurodegeneration in Alzheimer’s disease (AD), via the induction of free radical oxidative stress. Our results indicate that bpV(pic) provides protection against Aβ25-35-induced oxidative stress and neurotoxicity, suggesting that bpV(pic) could be a potential therapeutic candidate in the treatment of neurodegenerative diseases such as AD. © 2017 Informa UK Limited, trading as Taylor & Francis Group.


Sun J.,Pharmaceutical College | Dai X.,Pharmaceutical College | Li K.,Pharmaceutical College | Li H.,Pharmaceutical College
Speciality Petrochemicals | Year: 2010

Starting from N-methyl-4-piperidone and formaldehyde derivatives, the targeted compounds were synthesized with 10% alcoholic NaOH or dry hydrogen chloride as catalyst via aldol condensation reaction at room temperature for 7-8 h. The reaction was monitored by TLC. Three 3, 5-bis(aryli-dene)-4-piperidones were successfully synthesized with the yields above 49%. The structures were characterized by 1H NMR and melting points measurement. This was a convenient and efficient method with high yields. The compounds synthesized are used for antitumour evaluation.


Cui X.,Pharmaceutical College
Asian Journal of Chemistry | Year: 2014

An efficient surfactant assisted ultrasonic extraction technology was employed for extracting shikonin from Arnebia euchroma and the extraction process was optimized. Based on single-factor experiments, a four-factor-three-levels experimental design has been developed. The optimum conditions were carried out at 35 °C for 35 min at a solid-to-liquid ratio of 1:12 upon the addition of 0.0004 g L-1 surfactant. Shikonin contents could be determined readily by high-performance liquid chromatography within 20 min. The mean yield of shikonin was 30.64 mg L-1. Moreover, the contact angle of the powder was 30.501° in methanol. The proposed method was reliable by repeatability, stability and recovery tests. The results indicated that addition of surfactant could significantly increase the extraction yield of shikonin.


Panda S.K.,Pharmaceutical College | Das D.,Siksha ‘O’ Anusandhan University
Journal of Chemical and Pharmaceutical Sciences | Year: 2010

The present communication deals with the preliminary phytochemical studies on root tubers extract of Chlorophytum borivillianum Santapau & Fernandes known in commerce as 'Safed musli'. This plant of high economic importance due to mainly aphrodisiac & rejurnetive properties. No study report are available on the phytochemical studies of the roots. Hence, the present attempt has been undertaken to investigate the preliminary phytochemical properties. The study revealed the presence of more phytoconstituents as alkaloids, glycosides, steroids, saponins & flavonoides etc. in methanolic extract.


Zhang J.-W.,Pharmaceutical College | Yu W.-J.,Pharmaceutical College | Sheng X.-M.,Pharmaceutical College | Chang F.-H.,Pharmaceutical College | And 4 more authors.
Asian Pacific Journal of Cancer Prevention | Year: 2014

Purpose: To explore associations of CYP2E1 and NAT2 polymorphisms with lung cancer susceptibility among Mongolian and Han populations in the Inner Mongolian region. Materials and Methods: CYP2E1 and NAT2 polymorphisms were detected by PCR-RFLP in 930 lung cancer patients and 1000 controls. Results: (1) Disequilibrium of the distribution of NAT2 polymorphism was found in lung cancer patients among Han and Mongolian populations (p=0.031). (2) Lung cancer risk was higher in individuals with c1, D allele of CYP2E1 RsaI/PstI, DraI polymorphisms and slow acetylation of NAT2 (c1 compared with c2, OR=1.382, 95%CI: 1.178- 1.587, p=0.003; D compared with C, OR=1.241, 95%CI: 1.053-1.419, P < 0.001; slow acetylation compared with rapid acetylation, OR=1.359, 95%CI:1.042-1.768, p=0.056) (3) Compared with c2/c2 and rapid acetylation, c1/c1 together with slow acetylation synergetically increased risk of lung cancer 2.83 fold. (4) Smokers with CYP2E1 c1/c1, DD, and NAT2 slow acetylation have 2.365, 1.916, 1.841 fold lung cancer risk than others with c2/c2, CC and NAT2 rapid acetylation, respectively. (5) Han smokers with NAT2 slow acetylation have 1.974 fold lung cancer risk than others with rapid acetylation. Conclusions: Disequilibrium distribution of NAT2 polymorphism was found in lung cancer patients among Han and Mongolian populations. Besides, Han smokers with NAT2 slow acetylation may have higher lung cancer risk compared with rapid acetylation couterparts. CYP2E1 c1/ c1, DD and NAT2 slow acetylation, especially combined with smoking, contributes to the development of lung cancer. CYP2E1 c1/c1 or DD genotype and NAT2 slow acetylation have strong synergistic action in increasing lung cancer risk.

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