Ma X.-H.,Chengdu University of Traditional Chinese Medicine |
Ma X.-H.,Chinese Academy of Sciences |
Ma Y.,Chinese Academy of Sciences |
Ma Y.,Beijing Normal University |
And 11 more authors.
Molecules | Year: 2015
Secondary metabolites from plants play key roles in human medicine and chemical industries. Due to limited accumulation of secondary metabolites in plants and their important roles, characterization of key enzymes involved in biosynthetic pathway will enable metabolic engineering or synthetic biology to improve or produce the compounds in plants or microorganisms, which provides an alternative for production of these valuable compounds. Salvia miltiorrhiza, containing tanshinones and phenolic acids as its active compounds, has been widely used for the treatment of cardiovascular and cerebrovascular diseases. The biosynthetic analysis of secondary metabolites in S. miltiorrhiza has made great progress due to the successful genetic transformation system, simplified hairy roots system, and high-throughput sequencing. The cloned genes in S. miltiorrhiza had provided references for functional characterization of the post-modification steps involved in biosynthesis of tanshinones and phenolic acids, and further utilization of these steps in metabolic engineering. The strategies used in these studies could provide solid foundation for elucidation of biosynthetic pathways of diterpenoids and phenolic acids in other species. The present review systematically summarizes recent advances in biosynthetic pathway analysis of tanshinones and phenolic acids as well as synthetic biology and metabolic engineering applications of the rate-limiting genes involved in the secondary metabolism in S. miltiorrhiza. © 2015 by the authors; licensee MDPI, Basel, Switzerland.
Sun J.,Pharmaceutical College |
Dai X.,Pharmaceutical College |
Li K.,Pharmaceutical College |
Li H.,Pharmaceutical College
Speciality Petrochemicals | Year: 2010
Starting from N-methyl-4-piperidone and formaldehyde derivatives, the targeted compounds were synthesized with 10% alcoholic NaOH or dry hydrogen chloride as catalyst via aldol condensation reaction at room temperature for 7-8 h. The reaction was monitored by TLC. Three 3, 5-bis(aryli-dene)-4-piperidones were successfully synthesized with the yields above 49%. The structures were characterized by 1H NMR and melting points measurement. This was a convenient and efficient method with high yields. The compounds synthesized are used for antitumour evaluation.
Cui X.,Pharmaceutical College
Asian Journal of Chemistry | Year: 2014
An efficient surfactant assisted ultrasonic extraction technology was employed for extracting shikonin from Arnebia euchroma and the extraction process was optimized. Based on single-factor experiments, a four-factor-three-levels experimental design has been developed. The optimum conditions were carried out at 35 °C for 35 min at a solid-to-liquid ratio of 1:12 upon the addition of 0.0004 g L-1 surfactant. Shikonin contents could be determined readily by high-performance liquid chromatography within 20 min. The mean yield of shikonin was 30.64 mg L-1. Moreover, the contact angle of the powder was 30.501° in methanol. The proposed method was reliable by repeatability, stability and recovery tests. The results indicated that addition of surfactant could significantly increase the extraction yield of shikonin.
Panda S.K.,Pharmaceutical College |
Das D.,Siksha ‘O’ Anusandhan University
Journal of Chemical and Pharmaceutical Sciences | Year: 2010
The present communication deals with the preliminary phytochemical studies on root tubers extract of Chlorophytum borivillianum Santapau & Fernandes known in commerce as 'Safed musli'. This plant of high economic importance due to mainly aphrodisiac & rejurnetive properties. No study report are available on the phytochemical studies of the roots. Hence, the present attempt has been undertaken to investigate the preliminary phytochemical properties. The study revealed the presence of more phytoconstituents as alkaloids, glycosides, steroids, saponins & flavonoides etc. in methanolic extract.
Zhang J.-W.,Pharmaceutical College |
Yu W.-J.,Pharmaceutical College |
Sheng X.-M.,Pharmaceutical College |
Chang F.-H.,Pharmaceutical College |
And 4 more authors.
Asian Pacific Journal of Cancer Prevention | Year: 2014
Purpose: To explore associations of CYP2E1 and NAT2 polymorphisms with lung cancer susceptibility among Mongolian and Han populations in the Inner Mongolian region. Materials and Methods: CYP2E1 and NAT2 polymorphisms were detected by PCR-RFLP in 930 lung cancer patients and 1000 controls. Results: (1) Disequilibrium of the distribution of NAT2 polymorphism was found in lung cancer patients among Han and Mongolian populations (p=0.031). (2) Lung cancer risk was higher in individuals with c1, D allele of CYP2E1 RsaI/PstI, DraI polymorphisms and slow acetylation of NAT2 (c1 compared with c2, OR=1.382, 95%CI: 1.178- 1.587, p=0.003; D compared with C, OR=1.241, 95%CI: 1.053-1.419, P < 0.001; slow acetylation compared with rapid acetylation, OR=1.359, 95%CI:1.042-1.768, p=0.056) (3) Compared with c2/c2 and rapid acetylation, c1/c1 together with slow acetylation synergetically increased risk of lung cancer 2.83 fold. (4) Smokers with CYP2E1 c1/c1, DD, and NAT2 slow acetylation have 2.365, 1.916, 1.841 fold lung cancer risk than others with c2/c2, CC and NAT2 rapid acetylation, respectively. (5) Han smokers with NAT2 slow acetylation have 1.974 fold lung cancer risk than others with rapid acetylation. Conclusions: Disequilibrium distribution of NAT2 polymorphism was found in lung cancer patients among Han and Mongolian populations. Besides, Han smokers with NAT2 slow acetylation may have higher lung cancer risk compared with rapid acetylation couterparts. CYP2E1 c1/ c1, DD and NAT2 slow acetylation, especially combined with smoking, contributes to the development of lung cancer. CYP2E1 c1/c1 or DD genotype and NAT2 slow acetylation have strong synergistic action in increasing lung cancer risk.