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Choudhury S.,National Center for Sensor Research | Connolly D.,Pharmaceutical and Molecular Biotechnology Research Center | White B.,National Center for Sensor Research
Journal of Applied Polymer Science | Year: 2016

The application of polymeric high-internal-phase emulsion (polyHIPE) capillary coatings for open-tubular analytical separation columns was demonstrated in this study for the first time. Multiple polystyrene-co-divinylbenzene polyHIPE layers with an average total depth of 1.73 μm were coated onto internal capillary surfaces to create open-tubular columns (20 cm coating and 32.5 cm effective length). With these columns for open-tubular capillary electrochromatography, ethylbenzene and pentylbenzene were separated. Although the overall separation capacity of the produced columns was low, the polyHIPE coatings improved the analyte peak shape, decreased the total run time, and improved the peak symmetries relative to comparable unmodified open-tubular columns. In addition, the use of these novel polyHIPE columns led to the use of 30% less organic modifier. These columns have the potential to improve the shelf life of open-tubular columns typically used in capillary electrochromatography. © 2016 Wiley Periodicals, Inc.

Behl G.,Pharmaceutical and Molecular Biotechnology Research Center | Iqbal J.,Pharmaceutical and Molecular Biotechnology Research Center | O'Reilly N.J.,Pharmaceutical and Molecular Biotechnology Research Center | McLoughlin P.,Pharmaceutical and Molecular Biotechnology Research Center | Fitzhenry L.,Pharmaceutical and Molecular Biotechnology Research Center
Pharmaceutical Research | Year: 2016

Purpose: Dexamethasone sodium phosphate (DXP) is an anti-inflammatory drug commonly used to treat acute and chronic ocular diseases. It is routinely delivered using eye-drops, where typically only 5% of the drug penetrates the corneal epithelium. The bioavailability of such ophthalmic drugs can be enhanced significantly using contact lenses incorporating drug-loaded nanoparticles (NPs). Methods: The mechanism of release from chitosan NPs (CS-NPs), synthesized by ionic gelation, was studied in vitro. The DXP loaded CS-NPs were subsequently entrapped in contact lenses and the optical and drug-release properties were assessed. Results: DXP release from CS-NPs followed diffusion and swelling controlled mechanisms, with an additional proposed impact from the electrostatic interaction between the drug and the CS-NPs. The release rate was found to increase with an increase in drug loading from 20 to 50 wt%. However, an inverse effect was observed when initial loading increased to 100 wt%. NP-laden lenses were optically clear (95–98% transmittance relative to the neat contact lens) and demonstrated sustained DXP release, with approximately 55.73% released in 22 days. Conclusions: The release profile indicated that drug levels were within the therapeutic requirement for anti-inflammatory use. These results suggest that these materials might be a promising candidate for the delivery of DXP and other important ophthalmic therapeutics. © 2016 Springer Science+Business Media New York

Coady T.M.,Pharmaceutical and Molecular Biotechnology Research Center | Coffey L.V.,Pharmaceutical and Molecular Biotechnology Research Center | O'Reilly C.,Pharmaceutical and Molecular Biotechnology Research Center | Owens E.B.,Pharmaceutical and Molecular Biotechnology Research Center | Lennon C.M.,Pharmaceutical and Molecular Biotechnology Research Center
Journal of Molecular Catalysis B: Enzymatic | Year: 2013

Nitrile hydrolysing enzymes have found wide use in the pharmaceutical industry for the production of fine chemicals. This work presents a strategy that facilitates the rapid identification of bacterial isolates demonstrating nitrile hydrolysing activity. The strategy incorporates toxicity, starvation and induction studies along with subsequent colorimetric screening for activity, further focusing the assessment towards the substrates of interest. This high-throughput strategy uses a 96 well plate system, and has enabled the rapid biocatalytic screening of 256 novel bacterial isolates towards β-hydroxynitriles. Results demonstrate the strategy's potential to rapidly assess a variety of β-hydroxynitriles including aliphatic, aromatic and dinitriles. A whole cell catalyst Rhodococcus erythropolis SET1 was identified and found to catalyse the hydrolysis of 3-hydroxybutyronitrile with remarkably high enantioselectivity under mild conditions, to afford (S)-3-hydroxybutyric acid in 42% yield and >99.9% ee. The biocatalytic capability of this strain including the variation of parameters such as temperature and time were further investigated and all results indicate the presence of a highly enantioselective if not enantiospecific nitrilase enzyme within the microbial whole cell. © 2013 Elsevier B.V. All rights reserved.

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