PHARM REGION Ltd

Moscow, Russia

PHARM REGION Ltd

Moscow, Russia
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Nikiforova O.A.,Rutgers University | Klykov S.,Pharm Region Ltd. | Volski A.,Rutgers University | Dicks L.M.T.,Stellenbosch University | And 3 more authors.
Annals of Microbiology | Year: 2016

Bacillus subtilis KATMIRA1933, cultured in modified MRS (de Man, Rogosa, and Sharpe) broth without peptone (animal-free [AF]-MRS), produced subtilosin A at levels similar to, or even higher, than when cultured in MRS broth with peptone. AF-MRS medium contained 2.5 % (w/v) Martone or 2.5 % (w/v) cottonseed hydrolysate instead of peptone and 0.25 % (w/v) bacteriological grade yeast extract instead of normal yeast extract. An increase in cell numbers, accompanied by an increase in subtilosin activity, was recorded when cells were grown in AF-MRS supplemented with 0.4 % (w/v) K2HPO4 and 0.02 % (w/v) MgSO4. Subtilosin production increased from 30 arbitrary units (AU) mL−1 in a static culture to 320 AU mL−1 when cells were agitated on an orbital shaker at 300 rpm. A further increase in subtilosin production, from 150 AU mL−1 to 240 AU mL−1, was recorded when cells were cultured in AF-MRS supplemented with 2.0 % (w/v) amylopectin or 2.0 % (w/v) maltodextrin. Slightly higher cell densities were recorded in the presence of maltodextrin. Two colony types, one with a flat (“typical”), sprawling morphology and the other elevated (“atypical”), were isolated from cells grown in AF-MRS broth. Higher subtilosin levels (213 AU mL−1) were recorded from cells grown in AF-MRS broth supplemented with MgSO4 as compared to cells grown in the absence of MgSO4 (150 AU mL−1). © 2015, Springer-Verlag Berlin Heidelberg and the University of Milan.


Klykov S.P.,PHARM REGION Ltd | Kurakov V.V.,PHARM REGION Ltd | Vilkov V.B.,PHARM REGION Ltd | Demidyuk I.V.,Russian Academy of Sciences | And 2 more authors.
Biofabrication | Year: 2011

In this paper we have proposed a new structured population growth model, further developing a model previously proposed by the authors. Based on this model, optimal growth characteristics of the recombinant strain Escherichia coli BL-21 (DE3) [pProPlnHis 6] were determined, which allowed us to increase the output of metalloproteinase by 300%. We have experimentally demonstrated the applicability of the new model to cell cultures with implanted plasmids and the potential practical use for an output increase of a wide variety of biosynthesis processes. © 2011 IOP Publishing Ltd.


In this paper we have proposed a new structured population growth model, further developing a model previously proposed by the authors. Based on this model, optimal growth characteristics of the recombinant strain Escherichia coli BL-21 (DE3) [pProPlnHis(6)] were determined, which allowed us to increase the output of metalloproteinase by 300%. We have experimentally demonstrated the applicability of the new model to cell cultures with implanted plasmids and the potential practical use for an output increase of a wide variety of biosynthesis processes.

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