Klykov S.P.,PHARM REGION Ltd |
Kurakov V.V.,PHARM REGION Ltd |
Vilkov V.B.,PHARM REGION Ltd |
Demidyuk I.V.,Russian Academy of Sciences |
And 2 more authors.
Biofabrication | Year: 2011
In this paper we have proposed a new structured population growth model, further developing a model previously proposed by the authors. Based on this model, optimal growth characteristics of the recombinant strain Escherichia coli BL-21 (DE3) [pProPlnHis 6] were determined, which allowed us to increase the output of metalloproteinase by 300%. We have experimentally demonstrated the applicability of the new model to cell cultures with implanted plasmids and the potential practical use for an output increase of a wide variety of biosynthesis processes. © 2011 IOP Publishing Ltd.