Time filter

Source Type

Thị Trấn Ngọc Lặc, Vietnam

Toai T.C.,Pham Ngoc Thach Medical University | Thao H.D.,Pham Ngoc Thach Medical University | Thao N.P.,Pham Ngoc Thach Medical University | Gargiulo C.,Pham Ngoc Thach Medical University | And 3 more authors.
Cell and Tissue Banking | Year: 2010

It is well accepted that human umbilical cord blood (UCB) is a source of mesenchymal stem cells (MSCs) which are able to differentiate into different cell phenotypes such as osteoblasts, chondrocytes, adipocytes, myocytes, cardiomyocytes and neurons. The aim of this study was to isolate MSCs from human UCB to determine their osteogenic potential by using different kinds of osteogenic medium. Eventually, only those MSCs cultured in osteogenic media enriched with vitamin D2 and FGF9, were positive for osteocalcin by RT-PCR. All these cells were positive for alizarin red, alkaline phosphatase and Von Kossa. The results obtained from RT-PCR have confirmed that osteogenesis is complete by expression of the osteocalcin marker. In conclusion, vitamin D 2, at least in vitro, may replace vitamin D3 as an osteogenic stimulator factor for MSC differentiation. © Springer Science+Business Media B.V. 2009.

Toai T.C.,Pham Ngoc Thach Medical University | Thao H.D.,Pham Ngoc Thach Medical University | Gargiulo C.,University of Western Australia | Thao N.P.,Pham Ngoc Thach Medical University | And 5 more authors.
Cell and Tissue Banking | Year: 2011

There have been many attempts to acquire and culture human keratinocytes for clinical purposes including from keratotome slices in media with fetal calf serum (FCS) or pituitary extract (PE), from skin specimens in media with feeder layers, from suction blister epidermal roofs' in serum-free culture and from human umbilical cord blood (hUCB) mesenchymal stem cells (MSCs) in media with skin feeder layers. Conversely this study was designed to investigate whether keratinocytes could be obtained directly from hUCB MSCs in vitro. It is widely established that mesenchymal stem cells from human umbilical cord blood have multipotent capacity and the ability to differentiate into disparate cell lineages hUCB MSCs were directly induced to differentiate into keratinocytes by using a specific medium composed of primary culture medium (PCM) and serum free medium (SFM) in a ratio 1:9 for a period of 7 days and tested by immunostain p63 and K1-K10. Cells thus cultured were positive in both tests, confirming the possibility to directly obtain keratinocytes from MSCs hUCB in vitro. © 2010 Springer Science+Business Media B.V.

Gargiulo C.,Pham Ngoc Thach Medical University | Thao H.D.,Pham Ngoc Thach Medical University | Tuan H.M.,Pham Ngoc Thach Medical University | Thuy T.T.T.,Pham Ngoc Thach Medical University | And 3 more authors.
IFMBE Proceedings | Year: 2010

Biomaterial such as corals is an excellent osteoconductive material to be used to bone human derived stem cells for clinical regenerative intent. Bone regeneration is often needed for multiple clinical purposes for instance in aesthetic reconstruction and regenerative procedures. In this paper we describe an approach that aim to provide fundamental information allowing a scientific biomechanical basis for use of natural coral scaffold to initiate mesenchymal stem cells into osteogenic differentiation process for transplant purposes. Isolated MSCs from human bone marrow were induced into osteoblasts by using an osteogenic medium enriched with two specific growth factors FGF9 and vitamin D2. Eventually, part of cultured MSCs were directly transferred and seeded onto coral scaffolds and induced to differentiate into osteoblasts and part were cultured in apposite flask for mesenchymal stem cell medium. Finally, our data indicated that hBM is a very reliable source of MSCs and that these cells may be easily differentiated into osteoblast and seeded into coral as optimal device for clinical application. © Springer-Verlag 2010.

Nguyen P.V.N.,Pham Ngoc Thach Medical University | Hong T.K.,Pham Ngoc Thach Medical University | Hoang T.,Pham Ngoc Thach Medical University | Nguyen D.T.,Pham Ngoc Thach Medical University | And 2 more authors.
BMC Public Health | Year: 2013

Background: Two previous surveys conducted in Ho Chi Minh City revealed an increasing prevalence of overweight and obese adolescents, from 5.9% in 2002 to 11.7% in 2004. From 2004 to 2010, the government set up and implemented health promotion programs to promote physical activity and good nutritional habits in order to prevent overweight and obesity in children and adolescents. Our study aimed to estimate the prevalence of overweight and obesity among adolescents in urban areas of Ho Chi Minh City in 2010. Methods. A representative sample of 1,989 students aged 11-14 years was selected using a multistage cluster sampling method. 23 schools were randomly selected from the full list of all public junior high schools. In each selected school, 2 classes were chosen at random and all students from the class were examined. Age- and sex-adjusted overweight and obesity were defined using International Obesity Taskforce cut-offs. Results: The prevalences of overweight and obesity were 17.8% and 3.2%, respectively. Prevalences of overweight and obesity were significantly higher in boys (22%, 5.4%) than in girls (13.3%, 1.3%, p<0.001) and higher in children from districts with a high economic level (20.5%, 3.8%) than in those from districts with a low economic level (12.1%, 3.8%, p<0.001). Additionally, children living in wealthier families were more overweight and obese than those living in less wealthy families. When using WHO cutoffs, the overall prevalences of overweight and obesity reached 19.6% and 7.9%, respectively. Conclusion: Our study's findings suggest that the prevalence of overweight and obesity among secondary school students remains high, especially among boys living in wealthier families. Public health programs should therefore be developed or improved in order to promote good eating habits and physical activity among youth in HCMC. © 2013 Ngoc Nguyen et al; licensee BioMed Central Ltd.

Hoa N.T.,University of Oxford | Chieu T.T.B.,University of Oxford | Nghia H.D.T.,Pham Ngoc Thach Medical University | Mai N.T.H.,Hospital for Tropical Diseases | And 8 more authors.
BMC Infectious Diseases | Year: 2011

Background: Streptococcus suis is an emerging zoonotic pathogen and is the leading cause of bacterial meningitis in adults in Vietnam. Systematic data on the antimicrobial susceptibility profiles of S. suis strains isolated from human cases are lacking. We studied antimicrobial resistance and associated resistance determinants in S. suis isolated from patients with meningitis in southern Vietnam.Methods: S. suis strains isolated between 1997 and 2008 were investigated for their susceptibility to six antimicrobial agents. Strains were screened for the presence and expression of tetracycline and erythromycin resistance determinants and the association of tet(M) genes with Tn916- like transposons. The localization of tetracycline resistance gene tet(L) was determined by pulse field gel electrophoresis and Southern blotting.Results: We observed a significant increase in resistance to tetracycline and chloramphenicol, which was concurrent with an increase in multi-drug resistance. In tetracycline resistance strains, we identified tet(M), tet(O), tet(W) and tet(L) and confirmed their expression. All tet(M) genes were associated with a Tn916-like transposon. The co-expression of tet(L) and other tetracycline resistance gene(s) encoding for ribosomal protection protein(s) was only detected in strains with a minimum inhibitory concentration (MIC) of tetracycline of ≥ 64 mg/L. Conclusions: We demonstrated that multi-drug resistance in S. suis causing disease in humans in southern Vietnam has increased over the 11-year period studied. We report the presence and expression of tet(L) in S. suis strains and our data suggest that co-expression of multiple genes encoding distinct mechanism is required for an MIC ≥ 64 mg/L to tetracycline. © 2011 Hoa et al; licensee BioMed Central Ltd.

Discover hidden collaborations