Manuhara Y.S.W.,Airlangga University |
Kristanti A.N.,Airlangga University |
Utami E.S.W.,Airlangga University |
Yachya A.,PGRI University of Adi Buana
Asian Pacific Journal of Tropical Biomedicine | Year: 2015
Objective: To increase biomass and saponin production in hairy root culture of Talinum paniculatum Gaertn. (T. paniculatum) in balloon-type bubble bioreactor (BTBB). Methods: Hairy roots which were collected from leaf explants of T. paniculatum were infected by Agrobacterium rhizogenes strain LB510. The hairy roots were cultivated at 400 mL Murashige and Skoog liquid medium without growth regulator (MS0) in 1. 000 mL BTBB. Each BTBB had 2 g hairy roots as initial inoculum and these cultures were treated with various concentrations of sucrose (3%, 4%, 5%, 6% w/v) and potassium nitrate (0.5, 1.0, 1.5 and 2.0 strength of MS medium). Cultures were maintained for 14 days. Fresh and dry weights of hairy roots at the end of culture were investigated. Results: Various concentrations of sucrose influenced the biomass accumulation of hairy roots. Maximum biomass was reached by MS medium supplemented with 6% sucrose and it was approximately threefold higher than control. Culture supplemented with potassium nitrate at 2.0 strength of MS0 could increase biomass accumulation of hairy roots until 0.14 g dry weight and it was almost threefold higher than control. However, the maximum saponin content was obtained by MS medium supplemented with 5% sucrose and 2.0 strength potassium nitrate of MS. Conclusions: Based on this research, those conditions can be used to produce biomass and saponin of hairy root of T. paniculatum in the large scale. © 2015 Hainan Medical University.
Ratnawati R.,Sepuluh Nopember Institute of Technology |
Ratnawati R.,PGRI University of Adi Buana |
Trihadiningrum Y.,Sepuluh Nopember Institute of Technology |
Juliastuti S.R.,Sepuluh Nopember Institute of Technology
Journal of Solid Waste Technology and Management | Year: 2016
Rumen content waste is generally dried and dumped into municipal landfill by the slaughterhouse (SH) in Indonesia. A composting process using anaerobic-anoxic-oxic (A2 /O) method was selected for treating the SH solid waste for its advantage in nitrogen removal. The objectives of this study were to investigate optimum each composting duration, and effect of dolomite addition for alkalinity control. Six standing reactors of 60 L capacity were used in this experinment. Fifty kg of rumen content was placed in each reactor. The A2 /O configuration periods of 25-10-15, 15-10-25, and 30-10-10 days were applied in this research. The composting process was conducted for 50 days. Aerobic condition was maintained using aeration rate of 0.5 L (kg-1 dry weight solid waste) min-1 . Anoxic condition was established by 60 minute aeration rate of 0.28 L (kg-1 dry weight solid waste) min-1 . The composting process was conducted for 50 days. This research showed that the optimum anaerobic-anoxic-oxic time period for composting the rumen content waste was 25-10-15 days. In this reactor, the moisture content was decreased from 83% to 72%. Temperature range was 29-34° C, pH values slighty fluctuated from 7.57 to 8.70. Inorganic nitrogen concentration decreased from 0.29 to 0.07%, and organic nitrogen concentration decreased from 2.04 to 1.19%. Dolomite addition did not give (P value>α, α = 0.05). This research proved that A A2O method is appropriate for treating solid waste with high nutrient contents.
Sopandi T.,PGRI University of Adi Buana |
Wardah A.,University of Surabaya |
Surtiningsih T.,Airlangga University |
Suwandi A.,Airlangga University |
Smith J.J.,Queensland University of Technology
Journal of Applied Microbiology | Year: 2013
Aims: This research sought to determine optimal corn waste stream-based fermentation medium C and N sources and incubation time to maximize pigment production by an indigenous Indonesian Penicillium spp., as well as to assess pigment pH stability. Methods and Results: A Penicillium spp. was isolated from Indonesian soil, identified as Penicillium resticulosum, and used to test the effects of carbon and nitrogen type and concentrations, medium pH, incubation period and furfural on biomass and pigment yield (PY) in a waste corncob hydrolysate basal medium. Maximum red PY (497·03±55·13mgl-1) was obtained with a 21:1 C:N ratio, pH 5·5-6·0; yeast extract-, NH4NO3-, NaNO3-, MgSO4·7H2O-, xylose- or carboxymethylcellulose (CMC)-supplemented medium and 12days (25°C, 60-70% relative humidity, dark) incubation. C source, C, N and furfural concentration, medium pH and incubation period all influenced biomass and PY. Pigment was pH 2-9stable. Conclusions: Penicillium resticulosum demonstrated microbial pH-stable-pigment production potential using a xylose or CMC and N source, supplemented waste stream cellulose culture medium. Significance and Impact of the Study: Corn derived, waste stream cellulose can be used as a culture medium for fungal pigment production. Such application provides a process for agricultural waste stream resource reuse for production of compounds in increasing demand. © 2012 The Society for Applied Microbiology.
Arrofiqi F.,Sepuluh Nopember Institute of Technology |
Arifin A.,Sepuluh Nopember Institute of Technology |
Indrajaya B.,PGRI University of Adi Buana
2015 International Seminar on Intelligent Technology and Its Applications, ISITIA 2015 - Proceeding | Year: 2015
This paper describes design and test of a wearable FES system for the purpose of improving the performance of gait in patients with post-stroke. The prototype system that was developed includes electrical stimulator and sensor systems. Electrical stimulator was designed to generate pulse train that was realized using non-isolated boost converter. Sensor system was designed to measure gait phases that was realized using FSR sensors and to measure lower limb joint angles that was realized using a fusion of gyroscope and accelerometer-based tilt angle sensor. In order to remove measurement error due to bias error of the gyroscope and fluctuation of tilt sensor, Kalman filter was used to estimate true lower limb joint angles. Each system was tested separately. Testing was done by measuring the stimulator's output on the tibialis anterior muscle stimulation in normal subjects. The characteristics of pulse train in accordance with the desired specifications and capable of producing contractions in the tibialis anterior muscle. Sensor system was tested to measure gait parameters in subjects who walk normally. Comparison of the measured data with existing research data, showed the same pattern of the signal, the magnitude value is still in the standard deviation value of comparative data. © 2015 IEEE.
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Unipa Siapkan Lahan 4.2 Hektar Untuk Pengembangan Mahasiswa 23/Mei/2016 SURABAYA- Dalam perayaan diesnatalis ke 45 tahun Universitas PGRI Adi Buana (Unipa) Surabaya melakukan gebrakan baru untuk pengembangan kampus ...